Single-cell network biology characterizes cell type gene regulation for drug repurposing and phenotype prediction in Alzheimer’s disease
Fig 2
Centrality analysis reveal hub gene changes of cell-type gene regulatory networks in AD.
(A) An upset plot showing overlaps between the top 10% genes with largest out-degree and (B) betweenness centralities. The filled dots in the center matrix indicate the comparison between the respective sets (along the x-axis), and the bars on the top show size of the intersection. Blue and red rows indicate control and AD, respectively. (C) Scatter plots showing normalized gene centralities distribution and (D) the distribution of in-degrees in microglial AD and control networks. Genes with large changes in in-degree between AD and control are labelled. (E) Visualization of the subnetwork of 9 TFs with high betweenness centrality in microglia. Grey circles around the periphery of the network indicate target genes. Symbols of the nine central TFs are shown and the rest hidden for clarity. Blue and red edges indicate interaction in the control and AD networks, respectively. (F) A dot plot showing enrichment of gene ontology biological processes (y-axis) among genes with the most extensive changes in the in-degree centrality across all cell types (x-axis). The dot size is set according to the FDR-corrected p values, as shown in the key.