Slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor
Fig 5
Sequence and phylogenetic analysis of the domains suggests a common evolutionary origin.
Panel A shows clustering of domain profiles. Each family shown in a different color, connections indicate similarity found with e-value below 1e−5. Two domains of the slipknotted protein are shown as orange stars. All families from CL0062 are shown as circles colored according to the family. The family IDs with known unknotted topology are highlighted in blue font. The one-domain proteins (PF03788 and PF04172) are shown as triangles and family ID are colored in magenta font. Two domains of PF05145 (CL0142) are shown as red squares. Panel B shows the phylogenetic tree of the domains. Color-coding of tree branches is the same as for domains clustering on the panel A. Additionally, colored background areas serve to group the tree branches according to some properties: 1) families with known unknotted topology are highlighted with blue; 2) the slipknotted family is highlighted in orange; 3) the families with unknown topology are highlighted in light gray; 4) the families of one-domain proteins are highlighted with magenta.