Validation and tuning of in situ transcriptomics image processing workflows with crowdsourced annotations
Fig 4
The in situ transcriptomics annotation pipeline was tested using RCA (Rolling Circle Amplification) images from an in situ sequencing (ISS) experiment in the starfish database.
Worker consensus annotations for RCA test image ISS_rnd0_ch3_z0, which contains 416 spots, achieved 92% precision and 89% recall based on expert consensus annotations. The Jaccard similarity index (intersection over union) between the consensus annotations and the expert annotations was 0.85.