Rigid-body fitting to atomic force microscopy images for inferring probe shape and biomolecular structure
Fig 1
A pseudo-AFM image generation scheme and the search algorithm.
(A) The AFM probe tip (grey) is modeled as a hemisphere connected to a circular frustum of a cone. The shape of tip is determined by the tip radius (r) and a half-apex angle of cone (θ). The pseudo-AFM image represents the height of the tip when the tip collides with any atom (red circle) of the target molecule. (B) The exhaustive search finds the best match in the discrete space made of the orientation and the translation of the target molecule and the radius and the half-apex angle of the probe tip.