Benchmarking of tools for axon length measurement in individually-labeled projection neurons
Fig 7
Mean absolute error for axon length estimation by planes-based stereology.
A. The mean absolute error (in % of the real axon length indicated by colors–see color bar) is shown for different values of the distance between planes and the step between sampling boxes, and for axon classes 1 to 4 (columns). Note that the color bar is the same for classes 1, 2, and 3. B. Accuracy of the mean error estimation as maximum error estimation at 95% confidence (in % of the real axon length indicated by colors; same color bar for classes 1, 2, and 3). C. Mean effort, quantized by the mean number of intersections, required to estimate the axon length with different values of the distance between planes and the step for axon classes 1 to 4. Colors denote the number of intersections (in logarithmic scale; color bar is the same for classes 1, 2, and 3). Mean intersection values were obtained through linear interpolation from estimation for distance values in 3, 6, 9,…,30 μm and step values in 70, 80,…,150 μm.