Benchmarking of tools for axon length measurement in individually-labeled projection neurons
Fig 4
Axon length correlations for projection-based stereology.
A. Correlation between 3D real length and 2D projection length, by axonal class. Red lines represent the mean estimated 3D real length (ordinary least squares), and light red shaded areas correspond to 95% confidence intervals (CI95%). Gray dots depict full samples comprising all planes measurements (XY, XZ and YZ, note the organization of the data in triplets). Global adjusted R-squared = 0.99. B. Estimated slopes (red dots) and CI95% (horizontal black bars) values for correlations in A. Slopes can be interpreted as coefficients (alpha) that multiply 2D projection values to obtain predictions of 3D real lengths. Note that all axon classes share a common set of potential alphas at a 95% confidence level (gray shaded area).