A novel stochastic simulation approach enables exploration of mechanisms for regulating polarity site movement
Fig 7
Association reactions at the membrane stabilize clusters by trapping polarity factors at the patch.
Patch centroid diffusivity (Dpatch) as a function of the total number of GEF molecules and k2a (A), k3 (B), k4a (C). The size of the dots reflects the magnitude of Dpatch as indicated in the legend at the right. For each case, the value of the other two rate constants is shown above the panel. (D-F) The amounts of Cdc42 and GEF at the patch are quantified as the total Cdc42T (black) and Cdc42T-GEF (blue) respectively for the corresponding points enclosed by dashed boxes in (A-C). (G-I) Dwell times at the patch of Cdc42T (black) and GEF (blue) for the corresponding points enclosed by dashed boxes in (A-C) (see Methods for details). Error bars are the standard deviation from estimations in 10 independent simulations.