From cells to tissue: How cell scale heterogeneity impacts glioblastoma growth and treatment response
Fig 8
Proliferation is reduced in recurrent tumors.
Upper: diagnosis and recurrent tumor specimens from 9 GBM patients stained with Ki-67 antibody indicating proliferating cells. Lower: pre-treatment and post-treatment proliferation index for the virtual cohort fit to size dynamics. Left: Representative pre and post Tx samples. For the patient samples, the labeling index is defined as the % of DAB-stained area out of the total nuclear area for each patient in the region of highest staining density. For the model, we assume that Ki67 is positive only in the last 20 hours of the cell cycle, which is counted as a % in the area of highest activity. Right: Ki67 index is shown with pre and post treatment variation and compared using a Wilcoxon matched-pairs signed rank test. Red line shows the identity line on plot correlating pre and post Tx samples.