Structural and molecular insight into the pH-induced low-permeability of the voltage-gated potassium channel Kv1.2 through dewetting of the water cavity
Fig 2
pKa estimates of H418 and acidic residues and two key residues in the Kv1.2 channel.
(A) The pKa estimates of H418 and the acidic residues in the pore domain of the closed Kv1.2 channels. (B) Multiple sequence alignment for the pore domains of the rat Kv1 subfamily. The cylinders above the sequences denote the secondary structural information of the four helical segments. The two key conserved residues E327 and H418 are highlighted in yellow and bold font. (C) Bottom and side views of the close pore domain of the Kv1.2 channel. The four subunits are represented by different colors in the ribbon diagram. The magnified view shows the key residues E327 and H418 and their neighbors in the inner helical bundle, which are represented by the licorice and Cα balls.