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Recentrifuge: Robust comparative analysis and contamination removal for metagenomics

Fig 2

Robust contamination removal.

This is a hypothetic example with 5 samples and 6 dominant taxa to illustrate how the algorithm works. The top and bottom part of the figure shows the absolute frequency of reads assigned to the taxa before and after the contamination removal, respectively. There are two control samples, not modified throughout the process. In the rest of specimens, the general contaminants (those taxa present in the controls and other samples, like ContaminantA and ContaminantB) are removed, except in case of crossover contamination: those taxa are kept in the source sample or samples (Sample1 here) and removed from other real samples (Sample2 and Sample3 in this example). The algorithm parameter ΞΎ is set to 2.

Fig 2

doi: https://doi.org/10.1371/journal.pcbi.1006967.g002