Probabilistic fluorescence-based synapse detection
Fig 8
Presynaptic and postsynaptic puncta adjacency.
The first row contains a cutout showing a PSD-95 punctum with a pixel highlighted in the center of the image. The second row contains synapsin cutouts with the search grid overlaid. For this example, K = 3, so shown is a 3 × 3 grid spanning 3 slices is shown. The brightest box is highlighted in green. Thus, the output value of the synaptic probability map at the pixel specific in the PSD-95 image is the average pixel value of the green box multiplied by the intensity value of the PSD-95 pixel.