Highly accessible AU-rich regions in 3’ untranslated regions are hotspots for binding of regulatory factors
Fig 2
RBP enrichment on 3’UTRs is significantly correlated for most RBP pairs.
a) Schematic representation of the z-score calculation for each RBP pair (top). Pearson correlation coefficients of cluster enrichments are calculated for each pair of proteins (purple line) in a distance from -200 to 200 nt and compared against the positional correlations obtained from shuffled clusters in the same region (dashed green lines show the top and bottom 5% random distributions). The random clusters are then used to calculate the z-score of the Pearson correlation coefficient at each position. The colors in the main panel represent the z-scores of the correlations and range from blue (lower z-score) to red (higher z-score). All against all positional correlations calculated in this way are summarized in the heatmap (bottom). For each RBP in a column we show the significance of the positional binding of each of the RBPs in the rows around its binding sites. b) Zoom on the positional correlations calculated around AGO2 binding sites. The Pearson correlation coefficients of cluster enrichments (left) and their z-scores (right) are shown. The strongest positional correlations are observed around AGO2 binding sites. On the right panel, the z-scores of the correlations are shown, row-normalized.