Precision of Readout at the hunchback Gene: Analyzing Short Transcription Time Traces in Living Fly Embryos
Fig 5
Inference results for fly data.
(A) Inferred values of Pon for different nucleus positions (A-Anterior, B-Boundary) and cell cycles. (B) Example of the mean connected autocorrelation function of the traces in cell cycle 13 from the boundary region of embryo 1 (blue dots, with shaded error region) and of the fitted Poisson-like (red), two-state (green) and cycle (black) promoter models. The fitted curves generated from the two-state and three state cycle model are almost superimposed. See S3 Fig for fits of all autocorrelation functions in both cell cycles and regions. (C) Inferred values of kon + koff using the two-state model. In (A) and (C), the standard error bars are calculated by performing the inference on 20 random subsets that take 60% of the original data. (D) Inferred values of kon and koff in the Anterior (red) and Boundary (blue) for the two-state model, in cell cycle 12 (circle) and cell cycle 13 (square). For each condition, 4 inferred values for 4 movies are shown. The dotted black line depicts the limit to inference coming from the time of τblock ∼ 6s it takes the polymerase to leave the transcription initiation site (kon + koff = 1/(6s)). The shaded areas represent the standard deviational ellipse of kon, koff for each cycle and each embryo. The axes of the ellipses are the eigenvalues of the covariance matrix, represented in the directions of the eigenvectors. (E-F) Two simulated trajectories of the promoter state with the inferred parameters in the Anterior (red) and Boundary (blue).