Site-Specific Phosphorylation of VEGFR2 Is Mediated by Receptor Trafficking: Insights from a Computational Model
Fig 8
Relative activation pY1175 and pY1214 varies as a function of VEGF immobilization and concentration.
A-C. Total VEGFR2 phosphorylated on at least one of Y951, Y1175, and Y1214 (pR2, A) and site-specific phosphorylation (pY1175, B and pY1214, C) at 5 min with varying koff,V·M. Note quantities of VEGFR2 phosphorylated on any residues in Rab11 endosomes are negligible. D. Ratio of pY1214 to pY1175 at 5 minutes with varying koff,V·M. E. Percent of surface V·R2 complexes that are bound to the matrix at t = 5 minutes. F-H. The ratio of pY1214-VEGFR2 to pY1175-VEGFR2 for Vs (F) and Vb (G). The ratio of this quantity for bound VEGF and soluble VEGF (Vb/Vs) is shown in (H). Solid line, [V] = 2 ng/mL; dashed line, [V] = 20 ng/mL; dotted line, [V] = 200 ng/mL. Soluble VEGF (Vs), blue lines; bound VEGF (Vb), green lines. For all lines, HUVEC receptor numbers were used.