PTEN Hopping on the Cell Membrane Is Regulated via a Positively-Charged C2 Domain
Figure 4
The analysis method for hopping molecules proposed in this study.
(A) Two coordinate systems are introduced. In the global coordinate system, O(X,Y,T), the time origin is the initial frame and the spatial origin is the lower-left corner of the image. In the coordinate system about the j-th molecule, oj(xj,yj,tj), the time origin is the vanished time and the spatial origin is the vanished position of the molecule. (B) Membrane-associating molecules are classified into two types: those rebinding to the membrane after hopping (hopping molecule) and those recruited from the cytoplasm (recruited molecule). (C) The spatial distribution of wild-type PTEN trajectories (blue) observed in a single cell. Scale bar, 5 µm. (D) The number of molecules appeared in a single cell after excitation of the fluorophore. Time interval, 1.666 sec. (E) Schematics of the simulation of hopping molecules. (F) Results of the rebinding probability estimated from simulated molecules. Data are mean +/− SD.