While innate behaviors are conserved throughout the animal kingdom, it is unknown whether common signaling pathways regulate the development of neuronal populations mediating these behaviors in diverse organisms. Here, we demonstrate that the Wnt/ß-catenin effector Lef1 is required for the differentiation of anxiolytic hypothalamic neurons in zebrafish and mice, although the identity of Lef1-dependent genes and neurons differ between these 2 species. We further show that zebrafish and Drosophila have common Lef1-dependent gene expression in their respective neuroendocrine organs, consistent with a conserved pathway that has diverged in the mouse. Finally, orthologs of Lef1-dependent genes from both zebrafish and mouse show highly correlated hypothalamic expression in marmosets and humans, suggesting co-regulation of 2 parallel anxiolytic pathways in primates. These findings demonstrate that during evolution, a transcription factor can act through multiple mechanisms to generate a common behavioral output, and that Lef1 regulates circuit development that is fundamentally important for mediating anxiety in a wide variety of animal species.
Humans, mice, fish, and even flies exhibit anxiety-like behavior despite the fact that their brain anatomy varies widely. This study reveals another common thread that runs through these diverse animals: the molecular origins of their shared behavior. Gene knockout experiments in mouse and zebrafish show that the molecular signal Wnt acts through the transcription factor Lef1 to inhibit anxiety in both species. The pathway is required for formation of anxiolytic neurons in a highly conserved brain region, the hypothalamus. From there, however, the process diverges. In the fish, the pathway triggers genes including corticotropin-releasing hormone binding protein (crhbp), but in mice the same pathway calls into action a different gene, Pro-melanin concentrating hormone (Pmch). By comparison, the fruit fly Drosophila activates crhbp, similar to zebrafish. Furthermore, CRHBP and PMCH show extraordinarily coordinated expression in the primate hypothalamus, indicating that they may act together downstream of Wnt and Lef1 to regulate human behavior. This work reveals the surprising finding that conserved signaling pathways can regulate common behavioral outputs through diverse brain circuits during evolution.
Citation: Xie Y, Kaufmann D, Moulton MJ, Panahi S, Gaynes JA, Watters HN, et al. (2017) Lef1-dependent hypothalamic neurogenesis inhibits anxiety. PLoS Biol 15(8): e2002257. https://doi.org/10.1371/journal.pbio.2002257
Academic Editor: Ronald Duman, Yale School of Medicine, United States of America
Received: February 16, 2017; Accepted: July 21, 2017; Published: August 24, 2017
Copyright: © 2017 Xie et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: RNA-seq raw data were deposited to the Gene Expression Omnibus with accession no. GSE98519. All other relevant data are within the paper and its Supporting Information files.
Funding: NIH (grant number R01 NS082645). Received by RID. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NIH (grant number R01 AI112579). Received by HHX. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NIH (grant number R01 NS085413). Received by KCB. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. DoD (grant number CDMRP PR130373). Received by KCB. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NIH (grant number R01 AI121080). Received by HHX. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: AdjP, adjusted P value; AGRP, agouti-related protein; Cartpt, CART prepropeptide; CRH, corticotropin-releasing hormone; dpf, days post-fertilization; CRHBP, corticotropin-releasing hormone binding protein; E, embryonic day; EPM, elevated plus maze; GTEx, Genotype-Tissue Expression; Hc, caudal hypothalamus; HPA, hypothalamic-pituitary-adrenal; IPA, Ingenuity Pathway Analysis; NPY, neuropeptide Y; OFT, open field test; P, postnatal day; pan, pangolin; PI, pars intercerebralis; PL, pars lateralis; PMCH, pro-melanin concentrating hormone; qPCR, quantitative real-time PCR; RNA-seq, RNA sequencing; RPKM, reads per kilobase of transcript per million mapped reads; Tacr3, tachykinin receptor 3; wt, wild-type
Recent work has demonstrated that innate behaviors can be highly conserved across diverse animal models . Individual neuronal populations that mediate these behaviors are specified during embryogenesis by transcription factors that can also be conserved across species . However, molecular signaling pathways that regulate the development of common behavioral circuits have not been identified. As brain anatomy and connectivity change through evolution, it is possible that a single pathway could act through diverse molecular and cellular targets to establish a single behavioral output, which is the ultimate constraint on gene function.
Wnt/ß-catenin signaling plays important evolutionarily conserved roles in brain development, and thus represents an ideal candidate pathway to link gene regulation with the evolution of behavioral circuits. The Wnt pathway acts through Tcf/Lef transcription factors , and both Wnt signaling and Lef1 are required for neurogenesis in the zebrafish hypothalamus , an evolutionarily ancient brain structure that regulates innate behaviors . However, the identity and behavioral function of Lef1-dependent hypothalamic neurons, and their degree of evolutionary conservation, are unknown. Here, we show that Lef1 is required for the differentiation of hypothalamic neurons that inhibit anxiety in both zebrafish and mice, but through divergent molecular and cellular mechanisms in the 2 species. Generation of neurons expressing corticotropin-releasing hormone binding protein (crhbp) requires Lef1 in zebrafish but not in mice, whereas neurons expressing Pro-melanin concentrating hormone (Pmch) are Lef1-dependent in mice but not in zebrafish. Furthermore, zebrafish and Drosophila have common Lef1-dependent crhbp expression in their respective neuroendocrine organs, consistent with an ancient conserved pathway that has diverged in mammals. Finally, the Genotype-Tissue Expression (GTEx) project  reveals a top-ranked positive correlation between CRHBP and PMCH in the human hypothalamus, suggesting co-expression and/or co-regulation. Both genes are also correlated with LEF1 expression in humans, and are expressed in the same region of the marmoset hypothalamus, consistent with a conserved regulatory pathway in primates. These findings suggest that the gene expression network regulated by a transcription factor can change during evolution while still generating a common behavioral output. Our data also suggest an anxiolytic role for Wnt signaling in the human hypothalamus, with potential implications for the etiology and treatment of anxiety disorders.
Lef1 is required for the differentiation of hypothalamic neurons in zebrafish
We sought to first characterize the earliest cellular defect in lef1 null zebrafish mutants , so that we could perform a transcriptome analysis at that stage to identify Lef1-dependent genes. Despite grossly normal morphology, mass, and brain size, lef1 mutants have a smaller caudal hypothalamus (Hc) at 15 days post-fertilization (dpf) , and we found that the size reduction occurred at as early as 3–4 dpf (Fig 1A and S1A and S1B Fig). At 3 dpf the tissue already contained fewer Wnt-responsive cells  (Fig 1B), as well as fewer serotonergic cells and ventricular GABAergic HuC/D+ neurons (Fig 1C and S1C Fig). However, th2:GFP+ dopaminergic neurons  were unaffected (S1D Fig), indicating that not all neuronal subtypes are Lef1-dependent. In addition, the number of BLBP+ cells was increased (S1E Fig), confirming an inhibitory role of Wnt signaling in the formation of hypothalamic radial glia [4,9].
(A) Estimation of Hc size in control and lef1 mutants. See S1B Fig for method. (B-F) Immunostaining and quantification in 3 days post-fertilization (dpf) Hc. Representative immunostaining images of Wnt-responsive Tg(top:GFP)+ (B), 5-HT+ and HuC/D+ (C), and mitotic phospho-histone H3-positive (pH3+) cells (E) in control and lef1 mutants are shown on the left and quantified on the right (B1, C1, C2 and E1). Quantification of apoptotic active Caspase3+ (Cas3+) cells on the p53 mutant background is shown in (D), and representative immunostaining images of HuC/D+ cells are shown in (F). (G) Transplantation (schematic on the left) followed by HuC/D immunostaining at 5 dpf. All yellow rectangles depict the region with ventricular HuC/D+ cells normally present in wild-type (wt) fish, and magnified images in (G). All images show ventral views of whole-mounted brain with anterior on top. Data are mean ± SEM, except mean ± SD in (A). ***P < 0.001, **P < 0.01, *P < 0.05, ns. P > 0.05 by unpaired Student t tests. Scale bars: 25 μm. See S1 Table for description of confocal imaging, quantification and experimental n. Raw data can be found in S1 Data.
To determine the cellular mechanism underlying the decreased populations in lef1 mutants, we measured apoptosis and proliferation. We observed an increase in p53-dependent apoptosis within the Hc at 3 dpf (Fig 1D), but no change in proliferation at 3 dpf and beyond (Fig 1E and S1F–S1H Fig). Rescue of apoptosis by loss of p53 (Fig 1D) did not restore HuC/D expression in lef1 mutants (Fig 1F), consistent with a primary defect in progenitor differentiation. To confirm a failure in neurogenesis, we performed BrdU pulse-chase experiments, and observed fewer newly born serotonergic and ventricular HuC/D+ cells in lef1 mutants (S1I Fig). To test whether Lef1 functions cell-autonomously, we transplanted cells from lef1+/- donors into the hypothalamic anlage of lef1 mutant hosts during gastrulation, and observed rescue of ventricular HuC/D expression only in donor cells (Fig 1G). Together these data suggest that Lef1 functions cell-autonomously to promote hypothalamic neurogenesis; in lef1 mutants, neural progenitors fail to differentiate and subsequently undergo cell death, leading to a smaller Hc. Our data also justified 3 dpf as the optimal time point to perform a transcriptome analysis.
Lef1-dependent genes in the zebrafish hypothalamus are associated with anxiety
To identify Lef1-dependent genes, we next performed RNA sequencing (RNA-seq) analysis of whole hypothalami dissected from 3 dpf control and lef1 mutant zebrafish embryos, and found 144 genes with an adjusted P value (AdjP) <0.1, among which 53 genes had a fold change >2 (Fig 2A, S2 Table). Most of these genes had reduced expression in lef1 mutants (Fig 2A), consistent with Lef1 functioning as a Wnt transcriptional activator . Surprisingly, Ingenuity Pathway Analysis (IPA) identified Lef1-dependent genes as being most highly associated with anxiety and depressive disorder (Fig 2B and S3 and S4 Tables). In contrast, genes associated with other hypothalamus-mediated behaviors, such as feeding (neuropeptide Y [npy], agouti-related protein [agrp], and proopiomelanocortin [pomc]) or sleep (hypocretin [hcrt]), were unaffected (S2 Table). We performed in situ hybridization on 3 dpf offspring of lef1+/- incrosses and confirmed that all Lef1-dependent genes with specific detectable hypothalamic expression showed predicted changes in approximately 25% of embryos, consistent with Mendelian segregation (Fig 2C and 2D and S2A–S2C Fig). These included several known Wnt targets such as sp5a and sp5l  (Fig 2C), and anxiety-related genes identified from IPA (Fig 2B and 2D). Expression of neuronal markers such as crhbp and 5-hydroxytryptamine receptor 1A b (htr1ab), was lost specifically in the Hc of lef1 mutants while remaining intact in the rostral hypothalamus (Fig 2D), resulting in their relatively small fold change in whole hypothalamus RNA-seq analysis (S2 Table). In contrast, expression of other genes, such as 2 phosphodiesterase 9a (pde9a) paralogs, was lost in the rostral hypothalamus and Hc of lef1 mutants (Fig 2D and S2A Fig), consistent with lef1 expression in both regions (Fig 2C). We also observed expression of Lef1-dependent genes in the Hc of wild-type (wt) adult zebrafish (S2D Fig), suggesting the presence of Wnt activity and Lef1-dependent neuronal populations throughout life. Together these results suggested that lef1 mutants might have an anxiety-related behavioral phenotype.
(A) Volcano plot of zebrafish RNA sequencing (RNA-seq) shows differentially expressed genes in the 3 days post-fertilization (dpf) hypothalamus of lef1 mutants compared to control. Only genes with adjusted P value (AdjP) <0.1 (green line) are shown. Genes with an absolute value of log2 ratio >1 (blue lines) are shown in red; others are shown in black. Node size represents the averaged fragments per kilobase of transcript per million mapped reads of a gene in the control. (B) Ingenuity Pathway Analysis (IPA) for zebrafish hypothalamic Lef1-dependent genes revealed 20 genes associated with anxiety and depressive disorder, listed in the table. (C and D) Representative images of whole mount in situ hybridization on 3 dpf control and lef1 mutant embryos for known Wnt targets (C) and genes associated with anxiety and depressive disorder (D). Red and yellow arrows indicate expression in caudal and rostral hypothalamus, respectively. Lateral (axin2, dkk1b, lef1, notum1a, crhbp, and grin2cb) or ventral (other genes) views were selected for optimal expression visualization. Scale bar: 100 μm.
Zebrafish lef1 mutants exhibit increased anxiety
lef1 mutants raised with siblings had decreased survival and size (S3A and S3C Fig). When separated at 15 dpf, mutants survived normally (S3B and S3C Fig), but were still smaller than control siblings at culture densities that maximized their growth (Fig 3A and S3D Fig), a phenotype potentially due to enhanced anxiety . We then performed a novel tank diving test to measure anxiety-related behavior . We found that lef1 mutant larvae had a longer latency to enter the upper half of a novel tank and spent less overall time in this zone during the initial exploration phase (Fig 3B and 3C and S1 Video), consistent with elevated anxiety. Notably, lef1 mutants travelled less distance during this phase, partially due to more frequent freezing behavior as indicated by increased time in immobility (Fig 3D and 3E and S1 Video), and again consistent with elevated anxiety. Importantly, lef1 mutants no longer displayed anxiety-related behavior after the exploration phase (Fig 3F). The body growth and anxiety phenotypes in lef1 mutants could be explained by reduced expression of multiple hypothalamic genes including crhbp (Fig 2D), which encodes a corticotropin-releasing hormone (CRH) inhibitor . However, pleiotropic phenotypes in zebrafish lef1 mutants [4,15] could also contribute to defects in growth or motor behavior. Therefore, we sought to create a tissue-specific mouse knockout model to examine the hypothalamic function of Lef1, and to determine whether it is evolutionarily conserved.
(A) Size of 30 days post-fertilization (dpf) fish when raised at 5 fish per tank separated by genotype. n = 25, 30 for control and mutant, respectively. (B-F) Novel tank diving test. Sixteen dpf larvae were analyzed between 1–3 minutes (C-E) or 4–6 minutes (F) after entering a novel tank. n = 9 for both controls and mutants. Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ns. P > 0.05 by unpaired Student t tests. Raw data can be found in S1 Data.
Hypothalamic Lef1 inhibits anxiety in mice
Lef1 is expressed in the mouse Hc from embryonic day (E) 10.5 to adulthood [16,17], and while previously characterized Lef1 null mutants exhibit postnatal lethality and a smaller body size, no hypothalamic phenotypes were reported [18,19]. We created a mouse hypothalamus knockout model using Nkx2-1Cre and Lef1flox alleles [20,21]. We also introduced the Cre reporter RosatdTomato  to create the conditional knockout allele Nkx2-1Cre/+;Lef1flox/flox;RosatdTomato/+ (herein referred to as Lef1CKO) and control littermates Nkx2-1Cre/+;Lef1flox/+;RosatdTomato/+ (herein referred to as Lef1CON), which were used for all experiments. We confirmed successful recombination by tdTomato expression (S5A Fig), and loss of hypothalamic Lef1 and Wnt reporter  expression in Lef1CKO mice (S5B and S5C Fig), which were viable, fertile, and morphologically indistinguishable from Lef1CON littermates. However, both male and female Lef1CKO mice gained weight more slowly after weaning (Fig 4A), similar to the phenotype we observed in zebrafish lef1 mutants (Fig 3A), and again consistent with elevated anxiety .
(A) Body weight of male Lef1CKO (CKO-M, n = 27) and female Lef1CKO (CKO-F, n = 26) compared to controls (CON-M, n = 27; CON-F, n = 26). (B) Elevated plus maze (EPM). (C) Open field test (OFT). In (B) and (C), n = 12, 9 for male CON, CKO. In (B), n = 11, 11 for female CON, CKO in estrus; n = 12, 11 for female CON, CKO in diestrus. In (C), n = 12, 6 for female CON, CKO in estrus; n = 11, 16 for female CON, CKO in diestrus. Data are mean ± 95% CI (A) or SEM (B and C). ***P < 0.001, **P < 0.01, *P < 0.05, ns. P > 0.05 by 2-way ANOVA with repeated measures (A, F(1, 26) = 22.2 for male and F(1, 25) = 8.842 for female) and unpaired Student t tests (B and C). Outliers depicted in black (C) were excluded using the Grubbs’ test (P < 0.05). Raw data can be found in S1 Data.
To directly measure anxiety-related behavior, we used an elevated plus maze (EPM) test and found that male Lef1CKO mice spent significantly less time in the open arms and more time in the closed arms (Fig 4B) despite normal mobility (S4A Fig). In an open field test (OFT), male Lef1CKO mice spent significantly less time in the center zone (Fig 4C) despite normal mobility (S4B Fig). These results are consistent with elevated anxiety in male Lef1CKO mice. We also observed enhanced anxiety specifically in OFT with estrous female Lef1CKO mice, but not with diestrous or all females, or with EPM testing of any females (Fig 4B and 4C and S4A and S4B Fig), likely due to reported variations in anxiety-related behavior between different sexes  and different behavioral assays . Together, these results suggest a conserved role of hypothalamic Lef1 in inhibiting anxiety.
Hypothalamic Lef1 is required for generation of Pmch+ neurons in mice
Consistent with the neurogenesis defect we observed in zebrafish, we found fewer HuC/D+ cells in the mouse hypothalamic ventricular zone in Lef1CKO embryos at E14.5 (Fig 5A). Importantly, this effect was restricted to coronal sections in which endogenous Lef1 is expressed (S5B Fig). To identify Lef1-dependent genes in the mouse hypothalamus, we performed RNA-seq analysis of hypothalami dissected from E14.5 Lef1CON and Lef1CKO embryos, and surprisingly identified only 1 protein-coding gene that mapped to a unique locus with an AdjP <0.1 and a fold change >2, Pmch (Fig 5B and S5 Table). Pmch expression normally overlaps with Lef1 in the premammillary hypothalamus, and extends into the lateral hypothalamus (Fig 5C) [17,26]. We confirmed loss of Pmch expression in E14.5 Lef1CKO embryos by quantitative real-time PCR (qPCR) and immunostaining (Fig 5D and S5D and S5E Fig). The only other significantly affected protein-coding gene identified by RNA-seq, Ribosomal Protein L34 (Rpl34) (Fig 5B, S5 and S6 Tables), is a repetitive processed pseudogene that could not be conclusively mapped to a single genomic locus, although one copy is located adjacent to Lef1.
(A) Immunostaining of HuC/D+ cells in the hypothalamic ventricular zone of E14.5 CON-M and CKO-M, with quantification shown on the right (n = 4). Images are z-projections of 16 μm confocal optical slices, shown with dorsal side on top, and higher magnification views of yellow squares in the insets. (B) Volcano plot of mouse RNA sequencing (RNA-seq) shows differentially expressed genes in the hypothalamus of CKO-M compared to CON-M at E14.5 (left) and P22 (right), using the same format as in Fig 2A. (C) E14.5 sagittal in situ hybridization images (www.genepaint.org) show expression of Lef1 (red arrows) and Pmch in the wild-type (wt) hypothalamus . (D) Quantitative real-time PCR (qPCR) analysis for male shows hypothalamic gene expression in E14.5 and P22 CKO-M relative to CON-M. (E) P22 coronal in situ hybridization images show expression of Pro-melanin concentrating hormone (Pmch), CART prepropeptide (Cartpt), and Tachykinin receptor 3 (Tacr3) in the lateral hypothalamus. 3V, third ventricle. Data are mean ± SEM. ***P < 0.001, ns. P > 0.05 by unpaired Student t tests. Scale bars: 400 μm in (C); 30 μm in (E). Raw data can be found in S1 Data.
Reduced Pmch expression in Lef1CKO embryos was unexpected because its orthologs were not significantly affected in RNA-seq analysis of zebrafish lef1 mutants (S2 Table). To determine if any Lef1-dependent genes were conserved with zebrafish later in development, we performed another RNA-seq analysis at postnatal day (P) 22, when Lef1CKO mice begin to exhibit a growth defect (Fig 4A). In this experiment, we identified only 2 affected protein-coding genes mapped to unique loci with an AdjP <0.1: Pmch and Tachykinin receptor 3 (Tacr3) (Fig 5B, S6 Table). Tacr3 is known to be co-expressed in Pmch+ neurons, along with CART prepropeptide (Cartpt) . We confirmed their reduced expression in the lateral hypothalamus of P22 Lef1CKO mice by qPCR and in situ hybridization (Fig 5D and 5E and S5E Fig), consistent with loss of Pmch+ neurons. Decreased body weight observed after ablating Pmch+ neurons [28,29] may therefore be related to an anxiolytic role for these cells , which is further supported by characterization of their inputs and activity .
Orthologs of multiple Lef1-dependent anxiety-related genes in zebrafish are expressed near Lef1 in the mouse hypothalamus, such as Pde9a and Nitric oxide synthase 1 (Nos1) at E14.5 , and Crhbp and Histidine decarboxylase (Hdc) in adults . However, RNA-seq analysis indicated that expression of these genes was Lef1-independent in mice (S5 and S6 Tables), and we confirmed this result for Crhbp by qPCR and in situ hybridization (Fig 5D and S5E and S5F Fig). In addition, we confirmed that expression of zebrafish pmch orthologs  does not depend on Lef1 at either 3 dpf or 15 dpf (S6A–S6C Fig). While we cannot rule out the possibility that our RNA-seq analysis of the mouse hypothalamus lacked the sensitivity to identify other conserved Lef1-dependent genes, it is clear that the identity of Lef1-dependent neurons relevant for anxiety differs between zebrafish and mice.
Lef1 dependence of crhbp expression is conserved between zebrafish and Drosophila
Interestingly, many Lef1-dependent genes in zebrafish encoding components of anxiety-mediating transmitter pathways, such as GABA, 5-HT, and CRH (Fig 2B), have a conserved function in Drosophila anxiety-like behavior . Therefore, we hypothesized that hypothalamic Lef1-dependent neurons in zebrafish may represent an evolutionarily ancient pathway. The Drosophila pars intercerebralis (PI) and pars lateralis (PL) represent neuroendocrine organs equivalent to the vertebrate rostral hypothalamus and Hc, respectively . In Drosophila, a single Lef/Tcf family member, pangolin (pan), functions as a Wnt activator [33,34]. Consistent with our hypothesis, we detected specific pan expression at stage 14 and the crhbp ortholog CG15537 expression at stage 16 in the Drosophila PL primordium  (Fig 6A–6C). Furthermore, we observed a loss of crhbp expression in the PL of pan mutants  at stage 16, despite intact expression in the PI and normal PL morphology (Fig 6C–6E). Drosophila crhbp in the PL may also be anxiolytic by inhibiting CRH/CRH-like diuretic hormone in the PI [1,32,35], thus these results support a relationship between neuroendocrine Lef1 function and the development of anxiolytic Crhbp+ neurons dating back to a common bilaterian ancestor. By contrast, Pmch is a vertebrate specific gene, and Lef1-dependent Pmch+ neuronal circuitry in mice may reflect a more recent mammalian divergence that co-evolved with new brain structures .
(A-E) Whole mount in situ hybridization for the lef1 ortholog pan (A) and crhbp (C and D), and immunostaining for the pars lateralis (PL) marker FasII  (B and E) were performed in Drosophila wild-type (wt) embryos (A-C) and offspring from a pan+/- incross (D and E). Percentage of embryos with representative phenotype is displayed in (D) (n = 142) and (E) (n = 25). Confocal z-projections are shown in (B) and (E). All are representative images for at least 3 embryos. Left images in (A) and (C) are lateral views with dorsal side on top, and the other images are dorsal views. All images have anterior side on the left. Red and yellow arrows indicate the PL and pars intercerebralis (PI), respectively. Scale bars: 150 μm.
Coordinated expression of PMCH and CRHBP in the human hypothalamus
Our animal models suggest that in humans Lef1 may also regulate the formation of Pmch+ and/or Crhbp+ hypothalamic neurons. To test this hypothesis, we compared the hypothalamic RNA-seq transcriptomes of 96 human individuals from the GTEx project  (S7 Table). Despite the fact that these data did not include prenatal samples, we found that expression of PMCH and CRHBP are both moderately correlated with LEF1, which is expressed at a relatively low level in the adult human hypothalamus (Fig 7A and 7B). Notably, PMCH and CRHBP were both within the top 100 LEF1-correlated genes, along with known Wnt targets such as Sal-like protein 4 (SALL4)  and SP5  (Fig 7C and S8 Table).
(A-G) Pearson correlations for hypothalamic gene expression among 96 postmortem human samples obtained from the Genotype-Tissue Expression (GTEx) project . All the Pearson’s r and P values were calculated between 2 genes, and displayed in the graphs or tables after sorting by r values. Correlation expression profiles are shown for gene pairs Pro-melanin concentrating hormone (PMCH) versus LEF1 (A), Corticotropin-releasing hormone binding protein (CRHBP) versus LEF1 (B), CRHBP versus PMCH (F), and Agouti-related protein (AGRP) versus Neuropeptide Y (NPY) (G), with reads per kilobase of transcript per million mapped reads (RPKM) at log10 scale used on both axes. Note that 1 data point (NPY: 0.1395; AGRP: 0) was not included in (G) due to the inability of plotting a 0 value on the logarithmic axis. Three tables of correlated genes for LEF1 (C), CRHBP (D), and PMCH (E) list the top 9 positively correlated genes plus selected genes, including those involved in canonical Wnt signaling labeled in red. See the full list in S8 Table.
In the course of this analysis, we noticed similar correlation profiles for CRHBP and PMCH (Fig 7A and 7B), suggesting a possible expression correlation between these 2 genes. Surprisingly, we found CRHBP and PMCH to be the most highly correlated genes with each other (Fig 7D–7F and S8 Table), a relationship that has never been reported previously. Among the top 200 PMCH- or CRHBP-correlated genes, we also found 2 Wnt ligands and 1 Wnt co-activator: R-Spondin 1 (RSPO1)  (Fig 7D and 7E). As a comparison, AGRP is the most highly correlated gene with Neuropeptide Y (NPY) (Fig 7G and S8 Table), consistent with their co-expression in the same hypothalamic neurons . Interestingly, while Pmch and Crhbp are expressed in different regions of the mouse hypothalamus , they are expressed in the same hypothalamic nuclei in another primate, the marmoset according to the Marmoset Gene Atlas (https://gene-atlas.bminds.brain.riken.jp). Importantly, the results of all our correlation analyses are recapitulated on GeneNetwork (www.genenetwork.org) , which imported an older version of GTEx’s datasets and calculated Pearson correlation across a population (See Materials and methods). Together these data suggest co-expression of PMCH and CRHBP in the primate hypothalamus and potential regulation by LEF1-mediated Wnt signaling in humans.
In this study, we demonstrate that Lef1-mediated hypothalamic Wnt signaling plays an evolutionarily conserved role in regulating the formation of anxiolytic neurons (See Fig 8 for summary). In zebrafish lef1 mutants, neural progenitors fail to differentiate and undergo apoptosis, resulting in a smaller Hc (alternatively named the hypothalamic posterior recess, the posterior part of the paraventricular organ, or the caudal zone of the periventricular hypothalamus [4,43,44]). Any or all of the 20 anxiety-related genes that are misregulated in the zebrafish mutant (Fig 2B) may contribute to the behavioral phenotypes that we observe. Likewise, our data do not conclusively prove that crhbp+ neurons, or indeed any individual Lef1-dependent neuronal populations, mediate the effect of Lef1 on anxiety. Such a conclusion would require either rescue of the lef1 mutant phenotype by restoration of missing neurons, or phenocopy by specific ablation of the cells. However, the specific loss of Pmch+ neurons in our mouse conditional knockout (Fig 5B), combined with the unexpected expression correlation between PMCH and CRHBP in the human hypothalamus (Fig 7F), is consistent with a common role for these 2 genes in behavior. While we also cannot rule out the possibility that Lef1 mutants may have other behavioral defects, genes that are known to regulate other hypothalamus-driven behaviors, such as Npy, Agrp, Pomc, and Hcrt, are unaffected in our mutants (S2, S5 and S6 Tables). In addition, pure assessment of other behaviors cannot distinguish a direct phenotype from an anxiety-related secondary phenotype.
Lef1-mediated Wnt signaling plays an evolutionarily conserved role in hypothalamic neurogenesis that inhibits anxiety. However, the underlying molecular and cellular mechanisms can vary between organisms.
While the major product of Pmch, melanin-concentrating hormone (MCH), is an anxiolytic factor in teleosts , studies in mammals have reported it to be either anxiolytic, anxiogenic or having no effect [46,47]. In addition, the Pmch propeptide makes at least 2 more neuropeptides, neuropeptide-glutamic acid-isoleucine (NEI), and neuropeptide-glycine-glutamic acid (NGE), which are also involved in stress response and anxiety . Germline Pmch mouse knockouts gain weight more slowly than controls, a phenotype originally attributed to decreased food intake . However, on a different background strain, the same group reported that the knockout mice were not hypophagic, while retaining a growth phenotype . Interestingly, all rodent models ablating Pmch [49–53] or Pmch+ neurons [28,29] exhibit a reduced growth rate. One possible underlying mechanism could be enhanced anxiety , which was not directly tested in any of these studies. Therefore, we hypothesize that in Lef1CKO mice, loss of hypothalamic Pmch+ neurons is responsible for elevated anxiety, leading to a secondary growth phenotype.
Our data suggest that the gene expression and neuronal subtypes dependent on Lef1 can change during evolution while maintaining a common behavioral output. While transcriptional networks can undergo rapid rewiring at the level of enhancer binding sites during yeast, insect and mammalian evolution [54,55], the direct transcriptional targets of Lef1 mediating hypothalamic neurogenesis are still unknown. We have identified Tcf/Lef consensus binding sites in zebrafish and mouse Crhbp and Pmch loci, but it remains important to determine whether these 2 genes are direct targets of Lef1, or are instead lost as a secondary result of neurogenesis defects in mutants. In either case, it will also be useful to understand the circuitry of Lef1-dependent neurons. While the targets of Crhbp+ neurons in Drosophila and zebrafish are unknown, the projections of Pmch+ neurons in the hypothalamus of mice and other mammals are well characterized, and the regulation of these circuits by Lef1 in these species may be linked to anatomical and functional expansion of target brain regions such as the cortex . Importantly, the coordinated expression of CRHBP and PMCH in the human hypothalamus suggests that they may be co-expressed in a single neuronal cell type.
Loss of other genes important for hypothalamic neurogenesis has been shown to affect behavior . Interestingly, mice lacking hypothalamic Dbx1 also exhibit a loss of Pmch+ neurons along with other populations . In that study, Lef1-expressing hypothalamic nuclei were hypothesized to regulate innate behaviors outside the hypothalamic-pituitary-adrenal (HPA) axis, partly due to the observation of expanded Wnt activity in Dbx1 knockout animals. However, because our work demonstrates that Lef1 is in fact required for the genesis of Pmch+ neurons and for HPA-related behaviors, an alternative explanation is that Dbx1 functions in a parallel pathway to Lef1.
Together these results identify Wnt signaling as a link between brain development and function that allows essential behaviors to be maintained even as anatomical structures change through evolution. In addition, given the function for hypothalamic Wnt signaling in regulating postembryonic zebrafish neurogenesis , and the continuous expression of Lef1 in the hypothalamus of fish (S2D Fig) and mammals  throughout life, it would be interesting to test a possible contribution to adult behavior using temporal conditional knockout models. While Wnt signaling in the mammalian hippocampus and nucleus accumbens has been associated previously with anxiety and depression [57,58], our data demonstrate a novel requirement for pathway activity in a brain region that is highly conserved throughout the vertebrate lineage, and may prove useful for the diagnosis and treatment of hypothalamus-related anxiety disorders.
Materials and methods
All experimental protocols were approved by the University of Utah Institutional Animal Care and Use Committee and were in accordance with the guidelines from the National Institutes of Health. Approval number: 16–09011. Zebrafish were euthanized by ice water immersion. Mice were euthanized by CO2 or ketamine/xylazine.
Zebrafish (Danio rerio) were bred and maintained in a 14:10 hour light/dark cycle as previously described . Zebrafish per tank were fed with similar amount of food and treated by the staff who were blinded to the experiments. Wt strains were *AB. The following mutant and transgenic strains were used: lef1zd11 , Tg(top:GFP)w25 , Tg(dlx6a-1.4dlx5a-dlx6a:GFP)ot1 , Tg(h2afv:GFP)kca6 , Tg(th2:GFP-Aequorin)zd201 , p53e7 . lef1-/- homozygous mutants were identified between 3 dpf and 10 dpf by DASPEI staining as described previously  and at or after 15 dpf by loss of caudal fin ; wt and heterozygous siblings were used as controls. All the zebrafish were from at least 1 single-pair breeding. Genotyping was done as described before for lef1zd11  and p53e7 , except primers used for lef1zd11 (forward primer: 5ʹ-CACTCTCTCCAGCCCAACATT-3ʹ, reverse primer: 5ʹ-TGTTACTGTTGGGACTGATTTCTG-3ʹ).
Male and female C57BL/6J mice (Mus musculus) were group-housed with 2–5 mice per cage in a reverse 12 hour light/dark cycle with ad libitum access to food and water. Mice were 19–20 and 15–20 weeks old at the time of behavioral tests for male and female animals, respectively. Ai9 reporter RosatdTomato (line 007905) , Nkx2-1Cre (line 008661) , and TCF/Lef:H2B-GFP mice (line 013752)  were purchased from Jackson Laboratories. Lef1flox/flox mice were provided by HHX . All strains were maintained on a C57BL/6J background except TCF/Lef:H2B-GFP mice, which were originally on a C57BL/6 × 129 background. Male Nkx2-1Cre/Cre;Lef1flox/+ and female Lef1flox/flox;RosatdTomato/tdTomato mice were used to generate conditional knockout (Lef1CKO: Nkx2-1Cre/+;Lef1flox/flox;RosatdTomato/+) and control (Lef1CON: Nkx2-1Cre/+;Lef1flox/+; RosatdTomato/+) offspring. Females breeders were maintained by inbreeding. Male breeders were maintained by interbreeding Nkx2-1Cre/Cre;Lef1+/+ and Nkx2-1Cre/Cre;Lef1flox/+ for no more than 5 generations to avoid potential artifacts caused by Cre homozygous inbreeding . In occasional litters, Ai9 reporter expression was observed throughout the body of approximately 10% of experimental animals, consistent with published literature ; such animals were not used for experiments. All the mice were from at least 3 litters unless otherwise noted. Sex at E14.5 was determined by genotyping by Jarid 1c . When generating experimental mice for body weight measurement and behavioral tests, each litter was culled to 8 pups at P0. Genotyping for RosatdTomato and TCF/Lef:H2B-GFP animals was done according to available Jackson Laboratory protocols for these strains. Genotyping for Nkx2-1Cre mice was done using primers for Cre recombinase detection (forward primer: 5ʹ-ATGCTTCTGTCCGTTTGCCG-3ʹ, reverse primer: 5ʹ-CCTGTTTTGCACGTTCACCG-3ʹ). Genotyping for Lef1flox mice was done using primers contributed by HHX (forward primer: 5ʹ-GCAGATATAGACACTAGCACC-3ʹ, reverse primer: 5ʹ-TCCACACAACTAACGGCTAC-3ʹ).
Canton-S wild-type and pan2 mutant (BL4759) Drosophila melanogaster strains were obtained from Bloomington Stock Center.
Zebrafish transplantation experiments
At the sphere stage, 10–50 blastula cells from donor embryos were transplanted using a glass micropipette into the dorsal side of shield stage host embryos, 20–40 degrees from the animal pole, representing the hypothalamus anlage . Embryos were then raised to 5 dpf for immunohistochemistry. Donor and host embryos were retained for genotyping to identify lef1 mutants.
Four dpf zebrafish embryos were incubated in E3 media containing 10 mM BrdU (Sigma-Aldrich, St. Louis, MO) at 28.5°C for indicated time before being washed in E3 media for at least 3 times.
Embryos and larvae were fixed in 4% paraformaldehyde (PFA) for 3 hours at room temperature (RT) or overnight (O/N) at 4°C followed by brain dissection. Brains were either dehydrated in methanol and stored at −20°C, or immediately processed for immunohistochemistry. For 3 dpf embryos, 5% sucrose was included in the fixative to ease dissection. Brains were treated with 0.5 U dispase (Gibco #17105–041) in 2% PBST (PBS/2% Triton X-100) for 60 minutes at RT. For BrdU, PCNA, pH3 or Caspase-3 staining, brains were washed in water for 5 minutes twice, followed by incubation in 2 N HCl for 60 minutes at RT, followed by 2 more water washes. Brains were then blocked in 5% to 10% goat serum in 0.5% PBST for 60 minutes at RT. Embryos were incubated in primary antibodies in block O/N at 4°C and secondary antibodies and Hoechst 33342 (Life Technologies, H3570) in block O/N at 4°C before mounting in Fluoromount-G (SouthernBiotech, Birmingham, AL) with the ventral hypothalamus facing the coverslip. Primary antibodies were all used at 1:500 dilution except as noted: chicken anti-GFP (Aves Labs, GFP-1020), rabbit anti-GFP (Molecular Probes, A11122), mouse anti-HuC/D (Molecular Probes, A21271), rabbit anti-5-HT (ImmunoStar, 541016), rabbit anti-pH3 (1:400, Cell Signaling, 9713), rabbit anti-active Caspase-3 (BD Pharmingen, 559565), rabbit anti-BLBP (Abcam, ab32432), mouse anti-PCNA (Sigma, P8825), and chicken anti-BrdU (ICL, CBDU-65A-Z). Secondary antibodies were all used at 1:500 dilution: goat anti-mouse Alexa Fluor 448 (Invitrogen, A11001), goat anti-rabbit Alexa Fluor 488 (Invitrogen, A11008), donkey anti-chicken Alexa Fluor 488 (Jackson ImmunoResearch, 703-545-155), goat anti-rabbit cy3 (Jackson ImmunoResearch, 111-165-003), goat anti-mouse cy3 (Jackson ImmunoResearch, 115-165-003), goat anti-mouse Alexa Fluor 647 (Invitrogen, A21235), goat anti-rabbit Alexa Fluor 647 (Invitrogen, A21244), and goat anti-chicken Alexa Fluor 647 (Invitrogen, A21449). Hoechst 33342 (1:10,000) was used to stain nuclei. All the primary antibodies were validated previously [4,67].
E14.5 embryo heads were dissected in PBS and fixed in 4% PFA at RT for 1.5 hours or O/N at 4°C. Brains were dissected and cryoprotected in 15% and then 30% sucrose, embedded in OCT, and stored at −80°C. Brains were cryosectioned at a thickness of 16 μm, air dried and stored at −80°C. Air-dried sections were then washed in PTW (PBS+0.1% Tween 20) 3 times, followed by permeabilization in 0.25% PBST for 5 minutes and blocking in 10% goat serum in PTW for 60 minutes. Sections were incubated in primary antibodies in blocking solution O/N at 4°C and secondary antibodies in blocking solution for 2 hours at RT, followed by Hoechst 33342 stain for 10 minutes at RT before mounting in Fluoromount-G. Antibodies used were as described above except rabbit anti-LEF1 (1:200, Cell Signaling, 2230), goat anti-PMCH (1:500, Santa Cruz, sc14509) and donkey anti-goat Alexa Fluor 647 (1:400, Invitrogen, A21447). All primary antibodies were validated by absence of staining in Lef1CKO animals. For HuC/D staining, incubation for 30 minutes in 0.5 U dispase was performed in 0.25% PBST.
Drosophila immunohistochemistry was performed as previously described  except that a fluorescent secondary antibody was used. Antibodies used were as described above except mouse anti-FasII (1:5, DSHB, 1D4), which was validated previously .
Probes for in situ hybridization
In situ hybridization probes were made by a clone-free method as described previously [69,70], with DNA templates purified using Zymo Research DNA Clean & Concentrator-5 kit. Primers were designed by Primer-BLAST  except for mouse genes with primer sequences available from the Allen Brain Atlas (ABA)  or GenePaint Atlas . A full list of primers used to make probes is in S9 Table. cDNA made from 3 dpf zebrafish embryos, P2, and P60 mouse hypothalamus, and adult Drosophila (gift from C. Thummel) was used as the initial template for PCR to generate T7 promoter-containing DNA. RNA probes for zebrafish lef1  and axin2  were previously described. The RNA probe for Drosophila pan was generated from the Drosophila Gene Collection T7 promoter-containing cDNA GM04312 .
Whole mount in situ hybridization: Zebrafish
Zebrafish whole mount in situ hybridization was performed as described previously  except that 15 dpf and adult zebrafish were fixed in 4% PFA O/N at 4°C followed by washing in PBS and brain dissection. All tissues were treated for 30 minutes with 10 μg/ml Proteinase K. Pigmented embryos were bleached in 1% H2O2/5% formamide/0.5× SSC O/N at RT after in situ hybridization. 3 dpf embryos and postembryonic brains were imaged in 100% glycerol and PBS, respectively. For automated whole mount in situ hybridization, all steps following probe hybridization and before color reaction were performed using a BioLane HTI (Intavis, Chicago, IL).
Section in situ hybridization: Mice
Twenty-five μm brain cryosections were collected and post-fixed as previously described  (http://developingmouse.brain-map.org/docs/Overview.pdf). In situ hybridization was then performed as described .
Whole mount in situ hybridization: Drosophila
Drosophila whole mount in situ hybridization was performed as described previously .
Body length: Zebrafish
Zebrafish from a single home tank were anesthetized using tricaine (Sigma-Aldrich, St. Louis, MO) in shallow water. Images were acquired of immobilized, non-overlapping fish with a ruler for scale. Body length was calculated by measuring the distance between the mouth and the anterior edge of the tail fin, using ImageJ.
Novel tank diving test
Five fish from lef1+/- incrosses were raised per tank starting at 5 dpf. lef1 mutants and controls were separated at 15 dpf. Novel tank diving tests  were performed on 16 dpf larvae during the early afternoon of the same days, before lef1 mutants start to display surfacing behavior at 20 dpf. Novel rectangular tanks (16.6 cm × 9.5 cm × 12.3 cm) were illuminated by a centered white light, and videos were acquired with a mounted Nokia Lumia 640 phone 1080p camera. For each experiment, single mutant and control larvae were netted and then removed simultaneously from their home cages and transferred to novel tanks with identical water volume. The order of netting mutant and control fish was rotated between trials. Videos were viewed in MPlayerX to manually analyze the latency of larvae to enter the upper half of the tank after initial sinking. Videos were then imported and analyzed using Ethovision XT version 11.5 (Noldus, Leesburg, VA) during the initial exploration phase, with a tracking period of 2 minutes beginning 1 minute after release into the novel tank to decrease water agitation resulting from netting. Videos were also analyzed after the initial exploration phase with a tracking period during the 4 to 6 minute interval. Tracks were analyzed for distance travelled, time in upper half of the tank and time of immobility.
Body weight: Mice
All pups were weaned at P21 immediately following the first weighing. Pups weighing less than 6.5 g were excluded from analysis. All mice were weighed during the morning of the same days of the following weeks.
Behavior tests: Mice
Group-housed mice were allowed to acclimate to the animal facility for behavioral tests 9 days after an on-campus transfer. Each mouse was handled daily for 2 minutes, during midmorning for 7 days before commencement of behavioral testing using the cupped hand method . To avoid behavioral variation caused by female estrous cycle , a vaginal lavage procedure was done after daily handling for estrous phase evaluation for 7 days, as previously reported . Female mice in their proestrus or estrus phases were collectively grouped as “Estrus” and females in their metestrus and diestrus phases were collectively grouped as “Diestrus.” All mice were acclimated to the behavior room for 1 hour under red light (69 lux) before commencement of tests. Open field and EPM behavioral tests were performed in order, once daily for 2 days, from 9 am to 5 pm. The experimenter was blinded to genotype.
Each mouse was placed in a circular plexiglass chamber (4.5” diameter × 3” height) located inside an illuminated (330 lux) circular open field arena (110 cm diameter) and allowed to acclimate for 1 minute to decrease movement bias resulting from experimenter handling. After 1 minute, the plexiglass chamber was removed from the arena, and the mouse was allowed to freely explore the arena for 10 minutes. Movement was video recorded and analyzed using Ethovision version 9 (Noldus, Leesburg, VA).
The EPM apparatus was elevated 60 cm from the floor, having 2 open arms (35 cm × 5 cm) and 2 closed arms (35 cm × 16 cm) connected by a central platform (5 cm × 5 cm). The EPM was illuminated by a white light (205 lux) at the center platform. Each mouse was placed in a rectangular opaque white plexiglass chamber (2” × 3” × 5”) located on the center platform, and allowed to acclimate for 1 minute before commencement of the test. The white chamber was removed and the mouse was allowed to freely explore the EPM for 5 minutes. Behavior was video recorded and analyzed using Ethovision version 9 (Noldus, Leesburg, VA).
Embryos were fixed for 1.5 hours in 4% PFA/5% sucrose in PBS at RT, followed by whole hypothalamus dissection with super-fine forceps (FST, 11252–00). For each biological replicate, 28 to 38 dissected hypothalami were pooled for lef1 mutant and control samples from at least 1 single-pair breeding. RNA was extracted using a RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Ambion, AM1975) according to the manufacturer’s instructions. Three biological replicates were obtained on different days from offspring of different breedings. A total of 300 ng RNA per sample was submitted to the High Throughput Genomic Core at the University of Utah for RNA quality control by High Sensitivity R6K ScreenTape, RNA concentration by vacuum drying, cDNA library prep by Illumina TruSeq Stranded RNA Kit with Ribo-Zero Gold and sequencing by HiSeq 50 Cycle Single-Read Sequencing version 3. RNA-seq was analyzed by the Bioinformatics Core at the University of Utah. A transcriptome reference was created by combining GRCz10 chromosome sequences with Ensembl build 84 splice junction sequences generated with USeq (version 8.8.8) MakeTranscriptome. RNA-seq reads were mapped to the GRCz10 zebrafish transcriptome reference using Novoalign (version 2.08.03). Splice junction alignments were converted back to genomic space using USeq SamTranscriptomeParser. USeq DefinedRegionDifferentialSeq was used to generate per gene read counts, which were used in DESeq2 to determine differential expression. RNA-seq graph in Fig 2A was made by IPython Notebook with package NetworkX.
E14.5 and P22 nonweaned male Lef1CON and Lef1CKO hypothalami were dissected using a fluorescent microscope in ice-cold PBS, while tail tissue was retained for genotyping. E14.5 tissues were immediately immersed in RNAlater (Thermo Fisher, Waltham, MA) and stored at 4°C for up to 7 days until RNA extraction. P22 tissues dissected from at least 2 litters were immediately homogenized in TRIzol (Thermo Fisher, Waltham, MA) and stored at −80°C. Three biological replicates were prepared from either 5 pooled hypothalami (E14.5) or a single hypothalamus (P22) from Lef1CON and Lef1CKO mice, and RNA was extracted on the same day using TRIzol followed by purification with an RNeasy Mini Kit (Qiagen, Hilden, Germany) and on-column DNase digestion (Sigma-Aldrich, St. Louis, MO). One μg of RNA per sample was submitted to the High Throughput Genomic Core at the University of Utah for RNA quality control with Agilent RNA ScreenTape, cDNA library prep with Illumina TruSeq Stranded RNA Kit with Ribo-Zero Gold, and sequencing using HiSeq 50 Cycle Single-Read Sequencing version 4. RNA-seq reads were mapped to GRCm38. Differential gene expression analysis and graph plotting were carried out using the same methods as for zebrafish RNA-seq.
Three biological replicates of RNA from male and female mice were prepared as described above for RNA-seq. Two and a half μg RNA was used for cDNA synthesis with a SuperScript III Reverse Transcriptase kit (Invitrogen, Carlsbad, CA). qPCR was performed in triplicate using Platinum SYBR Green master mix (Invitrogen, Carlsbad, CA) on 96-well CFX Connect (Bio-Rad, Hercules, CA) plates or 384-well QuantStudio 12K Flex (Life Technologies, Durham, NC) plates at the Genomics Core at the University of Utah, according to manufacturer’s instructions. Gapdh was used to normalize quantification, and reverse transcriptase was omitted for controls. qPCR analysis was performed with the ΔΔCt method to determine relative expression change . Dissociation curve analysis was performed to confirm the specificity of amplicons. qPCR primers were designed from PrimerBank  as follows (forward primer first, reverse primer second, in 5ʹ to 3ʹ orientation with PrimerBank ID in the parentheses), Pmch (12861395a1): GTCTGGCTGTAAAACCTTACCTC, CCTGAGCATGTCAAAATCTCTCC; Tacr3 (10946720a1): CTGGGCTTGCCAGTGACAT, CGCTTGTGGGCCAAGATGAT; Crhbp (162287189c2): CTTACCCTCGGACACTTGCAT, GGTCTGCTAAGGGCATCATCT.
Image analysis and cell counting
Fluorescent images of dissected zebrafish and mouse brains were obtained with an Olympus FV1000 confocal microscope at the Cell Imaging Core at the University of Utah. Z-stack images were all maximum intensity z-projections of 3 μm slices; single- or double-labeled cells were manually counted in FV1000 ASW 4.2 Viewer. All the zebrafish and mouse in situ hybridization images were obtained with an Olympus SZX16 dissecting microscope except those in Fig 5E, S2C Fig and S6B Fig, which were obtained with an Olympus BX51WI compound microscope. Two months post-fertilization (mpf) zebrafish images (S3A and S3B Fig) were acquired using a Leica MZ16 microscope. Drosophila in situ hybridization images were obtained with a Zeiss Axioskop.
IPA (QIAGEN, Redwood City, CA) was performed with 129 mouse orthologs of the 138 zebrafish protein-coding genes identified from RNA-seq with AdjP <0.1 (S4 Table). Analysis was performed by the Bioinformatics Core at the University of Utah according to QIAGEN's instructions and “diseases and functions” were extracted from the software (S3 Table).
Human correlation analysis
Publically available GTEx raw datasets were downloaded from www.gtexportal.org in April 2017 as a single file: GTEx_Analysis_v6p_RNA-seq_RNA-SeQCv1.1.8_gene_rpkm.gct.gz. Ninety-six hypothalamic samples were identified according to their specific strong PMCH expression, and extracted into S7 Table by IPython Notebook with packages gzip and xlwt. Pearson correlation was calculated by gene reads per kilobase of transcript per million mapped reads (RPKM) using IPython Notebook with function scipy.stats.stats.pearsonr, followed by result writing into S8 Table by IPython Notebook with package xlwt. The same Pearson correlation r values were confirmed using Excel’s CORREL function. A similar correlation result was obtained when searching for the top 200 correlated genes by Pearson on GeneNetwork (www.genenetwork.org) in April 2017. Several differences are noted between our analyses and GeneNetwork’s analyses. First, GeneNetwork imported an older version of GTEx’s datasets (GTEXv5 Human Brain Hypothalamus RefSeq [Sep15] RPKM log2). Second, GeneNetwork calculated Pearson correlation using RPKM log2 rather than RPKM in our case. Third, GeneNetwork calculated Pearson’s sample correlation across a population, with an adjustment across the genome, and also based on the number of the top correlated genes requested by the users; in our case, we calculated Pearson correlation between 2 genes, and simply ranked all the genes by their Pearson’s r values calculated for the gene of interest. Lastly, GeneNetwork’s imported older GTEx datasets had 102 hypothalamic samples, 6 among which were left out in current GTEx’s server. The complete overlapping of the 96 samples further confirmed our successful extraction of hypothalamic datasets from the GTEx project.
No statistical methods were used to predetermine sample size. For behavioral assays, sample size was determined based on accepted practice. The experiments were not randomized. Due to visible phenotypes, the investigators were not blinded to outcome assessment except for whole mount in situ hybridization of zebrafish lef1+/- incrosses, Drosophila pan+/- incrosses, and mouse body weight and behavioral assays. Two-tailed unpaired Student t tests were performed for all statistical analysis, except mouse body weight (2-way ANOVA with repeated measures), using GraphPad Prism software version 6. Outliers were identified by Grubbs’ test for behavioral assays with significance assigned at P < 0.05 (alpha = 0.01). All the criteria for excluding data points were established prior to data collection.
S1 Fig. Lef1 promotes neurogenesis in the zebrafish caudal hypothalamus (Hc).
(A and B) Hc size in control and lef1 mutants (A) estimated by the area of confocal ventricular slice (B). Hc was defined as an oval indicated by red outline in (B). The lengths of a1, a2, b1, b2 in the representative image (B) were measured by ImageJ, and the area of the oval was calculated by the following equations: Estimated area = π*a*b/4; a = a1+a2; b = (b1+b2)/2. (C) Co-immunostaining of HuC/D and GABAergic lineage marker dlx5/6:GFP  in the 3 dpf Hc. Three confocal channel-split magnified images of the region depicted by the yellow rectangle are shown on the right. A representative image is shown for at least 3 embryos tested. (D and E) Immunostaining of th2:GFP+ (D) and BLBP+ cells (E) in the Hc of 3 dpf control and lef1 mutant. Representative images are shown on the left, and quantifications are shown on the right. Higher magnification views of yellow rectangles in single channel are shown in the insets in (E). (F-H) Measurement of proliferation in the Hc of 5 dpf control and lef1 mutant as shown by pH3+ (F) and PCNA+ cells (G; representative image on the left and quantification on the right; cells adjacent to the horizontal ventricle were counted), and 1 day BrdU labeling (H; schematic on the left). (I), BrdU pulse-chase (schematic on the left) to measure birth of 5-HT+ and ventricular HuC/D+ cells after 4 dpf. Data are mean ± SEM, except mean ± SD in (A) and (I). ***P < 0.001, **P < 0.01, *P < 0.05, ns. P > 0.05 by unpaired Student t tests. All images are confocal ventricular slices. All scale bars are 25 μm except 12.5 μm in the magnified image in (C). See S1 Table for description of quantification and experimental n. Raw data can be found in S1 Data.
S2 Fig. Whole mount in situ hybridization for zebrafish Lef1-dependent genes identified from RNA-seq.
(A) Representative images of whole mount in situ hybridization on 3 dpf control and lef1 mutant embryos. Red and yellow arrows indicate gene expression in caudal and rostral hypothalamus, respectively. Lateral (adarb2, ccdc129, foxb2, klf17, mmp17b, and slc18a2) or ventral (other genes) views were selected for optimal expression visualization. (B) Quantification of expression following whole mount in situ hybridization on 3 dpf offspring from lef1+/- incrosses. Fifty to eighty-five embryos were analyzed per gene. (C) Images of 3 dpf control brains centered on Hc from ventral view. (D) Gene expression in the hypothalamus of 4 months post-fertilization (mpf) female wild-type zebrafish from ventral view. Representative images are shown in (C) and (D) for at least 2 samples tested. Images of ventral view have anterior on top; images of lateral view have dorsal on top and anterior on the left. Red dashed outlines in (C) and (D) depict the caudal hypothalamus. Scale bars: 0.1 mm in (A); 5 μm in (C); 0.2 mm in (D). Raw data can be found in S1 Data.
S3 Fig. Physiological and behavioral analysis of zebrafish lef1 mutants.
(A-C) Body size and survival rate of lef1 mutants under different culture conditions. Offspring of lef1+/- incrosses were either unsorted or sorted by genotype at 15 dpf, and raised at 25 fish per tank. Body length and number of surviving fish at 2 mpf are shown in (C) with representative pictures in (A) and (B) (lef1 mutants have no caudal fins ). (D) Body length of wild-type fish with different culture densities . Data are mean ± SEM. Raw data can be found in S1 Data.
S4 Fig. Mouse anxiety tests.
(A) Elevated plus maze. (B) Open field test. In (A) and (B), n = 12, 9 for male CON, CKO. In (A), n = 11, 11 for female CON, CKO in estrus; n = 12, 11 for female CON, CKO in diestrus. In (B), n = 12, 6 for female CON, CKO in estrus; n = 11, 16 for female CON, CKO in diestrus. Data are mean ± SEM. **P < 0.01, ns. P > 0.05 by unpaired Student t tests. Outliers depicted in black (B) were excluded from statistical analysis using the Grubbs’ test (P < 0.05). Raw data can be found in S1 Data.
S5 Fig. Cellular and molecular phenotypes in mouse Lef1CKO hypothalamus.
(A) P50 female Nkx2-1Cre/+;Lef1flox/+;RosatdTomato/+ (CON-F) expresses tdTomato in the hypothalamus. Bright field (left) and red fluorescence (right) ventral view images of the same brain with anterior on top are shown. Representative images are shown for at least 3 adult brains dissected. (B) Immunostaining for Lef1 in the hypothalamus of E14.5 Lef1CON (CON) and Lef1CKO (CKO). Coronal images are z-projections of 16 μm confocal optical sections, shown with dorsal side on top. Representative images are shown for at least 2 replicates tested. (C) Immunostaining for Wnt reporter TCF/Lef:H2B-GFP. Hypothalamic green fluorescence (below) views of yellow rectangles in bright field (above) view images of the same brain are shown, respectively. Images are whole mount ventral views with anterior side on top, acquired with the same setting for CON and CKO. Representative images are shown for at least 3 replicates tested. (D) Immunostaining for Pmch in the E14.5 hypothalamus. Higher magnification views of yellow rectangles are shown in the insets. Coronal images are z-projections of 16 μm confocal optical slices, shown with dorsal side on top. (E) qPCR analysis for female shows hypothalamic gene expression in E14.5 and P22 CKO-F relative to CON-F. Data are mean ± SEM. ***P < 0.001, ns. P > 0.05 by unpaired Student t tests. (F) Twenty-five μm coronal section in situ hybridization for Crhbp in the male P22 ventral premammillary and posterior hypothalamus, shown with dorsal side on top. Representative images are shown for at least 2 replicates tested. 3V: third ventricle. All scale bars are 100 μm except 500 μm in (F). Raw data can be found in S1 Data.
S6 Fig. Normal expression of pmch and pmchl in zebrafish lef1 mutants.
(A-C) Whole mount in situ hybridization images for pmch and pmchl (pmch, like) in the hypothalamus of 3 dpf (A and B) and 15 dpf (C) zebrafish control and lef1 mutant embryos. Images of dorsal views (anterior on top) and lateral views (dorsal on top and anterior on the left) of the same individual lef1+/- or lef1-/- fish were shown in (A). Representative ventral view images of 3 dpf lef1+/- (B), 15 dpf control and lef1 mutant (C) brains centered on the caudal hypothalamus (dashed red outlines) with anterior on top. Number of fish with representative gene expression among total number of fish is labeled on the right upper corner of each image in (C). Scale bar: 100 μm in (A and C); 5 μm in (B).
S1 Table. Details of confocal images, quantification and number of samples.
S3 Table. Ingenuity Pathway Analysis (IPA) for diseases & functions.
S4 Table. IPA input gene list.
Same zebrafish genes in Tab “AdjP<0.1” of S2 Table are listed with orthologous mouse gene information used for IPA.
S7 Table. Extracted GTEx RNA-seq data from 96 human hypothalamic samples.
S8 Table. Pearson correlation with hypothalamic GTEx RNA-seq data for PMCH, CRHRP, LEF1 and NPY.
Gene name followed with “_r” indicates Pearson’s r value and gene name followed with “_p” indicates P value.
S9 Table. Primer sequences for synthesizing in situ hybridization probes.
Reverse primers also included a T7 promoter-containing sequence “CCAAGCTTCTAATACGACTCACTATAGGGAGA” that was added 5' to the sequences listed in the table . All primers were designed by Primer-BLAST except mouse genes Cartpt (ABA experiment 72077479), Crhbp (ABA experiment 77455017), Pmch (GenePaint set MH227) and Tacr3 (ABA experiment 80342167).
S1 Data. Excel spreadsheet containing, in separate sheets, the underlying numerical data for all the figure panels.
We thank the University of Utah Centralized Zebrafish Animal Resource for zebrafish husbandry, and other University Core Facilities for assistance with DNA synthesis and sequencing, RNA-seq, bioinformatics analysis, qPCR, and genotyping. We thank J. Bonkowsky for providing zebrafish tracking software, R. Stewart and B. Link for providing fish lines, and E. Anne Martin for creating the cartoons in Fig 8. We thank J. Kiefer, A. Douglass, A. Johnson, M. Shapiro, F. Merkle, W. Huang, S. Blackshaw, and T. Shimogori for input on this manuscript. We thank A. Clark, EML, and the Cold Spring Harbor Laboratory Mouse Course for training YX in mouse experimental techniques, and S. Sakonju, H. Gordon, and Z. Yu for initial discussion of Drosophila experiments.
- 1. Mohammad F, Aryal S, Ho J, Stewart JC, Norman NA, Tan TL, et al. Ancient anxiety pathways influence Drosophila defense behaviors. Curr Biol. 2016;26(7):981–6. pmid:27020741
- 2. Xie Y, Dorsky RI. Development of the hypothalamus: conservation, modification and innovation. Development. 2017;144(9):1588–99. pmid:28465334
- 3. Nusse R, Clevers H. Wnt/β-catenin signaling, disease, and emerging therapeutic modalities. Cell. 2017 Jun 1;169(6):985–99. pmid:28575679
- 4. Wang X, Kopinke D, Lin J, McPherson AD, Duncan RN, Otsuna H, et al. Wnt signaling regulates postembryonic hypothalamic progenitor differentiation. Dev Cell. 2012 Sep 11;23(3):624–36. pmid:22975330
- 5. Löhr H, Hammerschmidt M. Zebrafish in endocrine systems: recent advances and implications for human disease. Annu Rev Physiol. 2011;73:183–211. pmid:21314433
- 6. Lonsdale J, Thomas J, Salvatore M, Phillips R, Lo E, Shad S, et al. The Genotype-Tissue Expression (GTEx) project. Nat Genet. 2013;45(6):580–5. pmid:23715323
- 7. Dorsky RI, Sheldahl LC, Moon RT. A transgenic Lef1/beta-catenin-dependent reporter is expressed in spatially restricted domains throughout zebrafish development. Dev Biol. 2002 Jan 15;241(2):229–37. pmid:11784107
- 8. McPherson AD, Barrios JP, Luks-Morgan SJ, Manfredi JP, Bonkowsky JL, Douglass AD, et al. Motor behavior mediated by continuously generated dopaminergic neurons in the zebrafish hypothalamus recovers after cell ablation. Curr Biol. 2015;26(2):263–9. pmid:26774784
- 9. Duncan RN, Xie Y, McPherson AD, Taibi A V, Bonkowsky JL, Douglass AD, et al. Hypothalamic radial glia function as self-renewing neural progenitors in the absence of Wnt/β-catenin signaling. Development. 2016 Jan 1;143(1):45–53. pmid:26603385
- 10. MacDonald BT, Tamai K, He X. Wnt/beta-catenin signaling: components, mechanisms, and diseases. Dev Cell. 2009;17(1):9–26. pmid:19619488
- 11. Weidinger G, Thorpe CJ, Wuennenberg-Stapleton K, Ngai J, Moon RT. The Sp1-related transcription factors sp5 and sp5-like act downstream of Wnt/beta-catenin signaling in mesoderm and neuroectoderm patterning. Curr Biol. 2005 Mar 29;15(6):489–500. pmid:15797017
- 12. Carr JA. Stress, neuropeptides, and feeding behavior: a comparative perspective. Integr Comp Biol. 2002;42(3):582–90. pmid:21708754
- 13. Cachat JM, Canavello PR, Elkhayat SI, Bartels BK, Hart PC, Elegante MF, et al. Video-aided analysis of zebrafish locomotion and anxiety-related behavioral responses. In: Zebrafish Neurobehavioral Protocols. 2011. p. 1–14.https://doi.org/10.1007/978-1-60761-953-6_1 pmid:27019459
- 14. Karolyi IJ, Burrows HL, Ramesh TM, Nakajima M, Lesh JS, Seong E, et al. Altered anxiety and weight gain in corticotropin-releasing hormone-binding protein-deficient mice. Proc Natl Acad Sci U S A. 1999;96(September):11595–600. pmid:10500222
- 15. Mcgraw HF, Drerup CM, Culbertson MD, Linbo T, Raible DW, Nechiporuk A V. Lef1 is required for progenitor cell identity in the zebrafish lateral line primordium. Development. 2011 Sep;138(18):3921–30. pmid:21862556
- 16. Ng L, Bernard A, Lau C, Overly CC, Dong H-W, Kuan C, et al. An anatomic gene expression atlas of the adult mouse brain. Nat Neurosci. 2009;12(3):356–62. pmid:19219037
- 17. Shimogori T, Lee DA, Miranda-Angulo A, Yang Y, Wang H, Jiang L, et al. A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010 Jul;13(6):767–75. pmid:20436479
- 18. van Genderen C, Okamura RM, Fariñas I, Quo RG, Parslow TG, Bruhn L, et al. Development of several organs that require inductive epithelial-mesenchymal interactions is impaired in LEF-1-deficient mice. Genes Dev. 1994 Nov 15;8(22):2691–703. pmid:7958926
- 19. Galceran J, Miyashita-Lin EM, Devaney E, Rubenstein JL, Grosschedl R. Hippocampus development and generation of dentate gyrus granule cells is regulated by LEF1. Development. 2000 Feb;127(3):469–82. pmid:10631168
- 20. Yu S, Zhou X, Steinke FC, Liu C, Chen S, Zagorodna O, et al. The TCF-1 and LEF-1 transcription factors have cooperative and opposing roles in T cell development and malignancy. Immunity. 2012 Nov 16;37(5):1–26. pmid:23103132
- 21. Xu Q, Tam M, Anderson SA. Fate mapping Nkx2.1-lineage cells in the mouse telencephalon. J Comp Neurol. 2008;29(September 2007):16–29. pmid:17990269
- 22. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, et al. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133–40. pmid:20023653
- 23. Ferrer-Vaquer A, Piliszek A, Tian G, Aho RJ, Dufort D, Hadjantonakis A-K. A sensitive and bright single-cell resolution live imaging reporter of Wnt/ß-catenin signaling in the mouse. BMC Dev Biol. 2010;10:121. pmid:21176145
- 24. Bale TL, Epperson CN. Sex differences and stress across the lifespan. Nat Neurosci. 2015;18(10):35–42. pmid:26404716
- 25. Clément Y, Calatayud F, Belzung C. Genetic basis of anxiety-like behaviour: A critical review. Brain Res Bull. 2002;57(1):57–71. pmid:11827738
- 26. Visel A, Thaller C, Eichele G. GenePaint.org: an atlas of gene expression patterns in the mouse embryo. Nucleic Acids Res. 2004;32(Database issue):D552–6. pmid:14681479
- 27. Brischoux F, Fellmann D, Risold PY. Ontogenetic development of the diencephalic MCH neurons: A hypothalamic “MCH area” hypothesis. Eur J Neurosci. 2001;13(9):1733–44. pmid:11359525
- 28. Alon T, Friedman JM. Late-onset leanness in mice with targeted ablation of melanin concentrating hormone neuron. J Neurosci. 2006;26(2):389–97. pmid:16407534
- 29. Whiddon BB, Palmiter RD. Ablation of neurons expressing melanin-concentrating hormone (MCH) in adult mice improves glucose tolerance independent of MCH signaling. J Neurosci. 2013;33(5):2009–16. pmid:23365238
- 30. González JA, Iordanidou P, Strom M, Adamantidis A, Burdakov D. Awake dynamics and brain-wide direct inputs of hypothalamic MCH and orexin networks. Nat Commun. 2016;7(c):11395. pmid:27102565
- 31. Berman JR, Skariah G, Maro GS, Mignot E, Mourrain P. Characterization of two melanin-concentrating hormone genes in zebrafish reveals evolutionary and physiological links with the mammalian MCH system. J Comp Neurol. 2009;517(5):695–710. pmid:19827161
- 32. Hartenstein V. The neuroendocrine system of invertebrates: a developmental and evolutionary perspective. J Endocrinol. 2006;190(3):555–70. pmid:17003257
- 33. Brunner E, Peter O, Schweizer L, Basler K. pangolin encodes a Lef-1 homologue that acts downstream of Armadillo to transduce the Wingless signal in Drosophila. Vol. 385, Nature. 1997. p. 829–33. pmid:9039917
- 34. Van de Wetering M, Cavallo R, Dooijes D, Van Beest M, Van Es J, Loureiro J, et al. Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF. Cell. 1997;88(6):789–99. pmid:9118222
- 35. Huising MO, Flik G. The remarkable conservation of corticotropin-releasing hormone (CRH)-binding protein in the honeybee (Apis mellifera) dates the CRH system to a common ancestor of insects and vertebrates. Endocrinology. 2005;146(5):2165–70. pmid:15718273
- 36. Croizier S, Cardot J, Brischoux F, Fellmann D, Griffond B, Risold PY. The vertebrate diencephalic MCH system: A versatile neuronal population in an evolving brain. Front Neuroendocrinol. 2013;34(2):65–87. pmid:23088995
- 37. Mele M, Ferreira PG, Reverter F, DeLuca DS, Monlong J, Sammeth M, et al. The human transcriptome across tissues and individuals. Science (80-). 2015 May 7;348(6235):660–5. pmid:25954002
- 38. Böhm J, Sustmann C, Wilhelm C, Kohlhase J, Bo J, Sustmann C, et al. SALL4 is directly activated by TCF/LEF in the canonical Wnt signaling pathway. Biochem Biophys Res Commun. 2006 Sep 29;348(3):898–907. pmid:16899215
- 39. Ardlie KG, Deluca DS, Segre A V., Sullivan TJ, Young TR, Gelfand ET, et al. The Genotype-Tissue Expression (GTEx) pilot analysis: Multitissue gene regulation in humans. Science (80-). 2015 May 7;348(6235):648–60. pmid:25954001
- 40. Jin Y-R, Yoon JK. The R-spondin family of proteins: emerging regulators of WNT signaling. Int J Biochem Cell Biol. 2012 Dec;44(12):2278–87. pmid:22982762
- 41. Schwartz MW, Hahn TM, Breininger JF, Baskin DG. Coexpression of Agrp and NPY in fasting-activated hypothalamic neurons. Nat Neurosci. 1998 Aug 1;1(4):271–2. pmid:10195157
- 42. Mulligan MK, Mozhui K, Prins P, Williams RW. GeneNetwork: A Toolbox for Systems Genetics. In: Methods Mol Biol. 2017. p. 75–120. pmid:27933521
- 43. Kaslin J, Panula P. Comparative anatomy of the histaminergic and other aminergic systems in zebrafish (Danio rerio). J Comp Neurol. 2001;440(4):342–77. pmid:11745628
- 44. Wullimann MF, Rupp B, Reichert H. Neuroanatomy of the zebrafish brain: A topological atlas. Birkhäuser Verlag. Basel: Birkhäuser Basel; 1996. 144 p.
- 45. Baker BI. Melanin-concentrating hormone: a general vertebrate neuropeptide. Int Rev Cytol. 1991;126:1–47. pmid:2050497
- 46. Griffond B, Baker BI. Cell and molecular cell biology of melanin-concentrating hormone. Vol. 213, Int. Rev. Cytol. Elsevier Masson SAS; 2002. 233–277 p. pmid:11837894
- 47. Chung S, Parks GS, Lee C, Civelli O. Recent updates on the melanin-concentrating hormone (MCH) and its receptor system: Lessons from MCH1R antagonists. J Mol Neurosci. 2011;43(1):115–21. pmid:20582487
- 48. Bittencourt J, Celis ME. Anatomy, function and regulation of neuropeptide EI (NEI). Peptides. 2008;29(8):1441–50. pmid:18456371
- 49. Shimada M, Tritos NA, Lowell BB, Flier JS, Maratos-Flier E. Mice lacking melanin-concentrating hormone are hypophagic and lean. Nature. 1998;396(December):670–4. pmid:9872314
- 50. Kokkotou E, Jeon JY, Wang X, Marino FE, Carlson M, Trombly DJ, et al. Mice with MCH ablation resist diet-induced obesity through strain-specific mechanisms. Am J Physiol Regul Integr Comp Physiol. 2005;289:R117–24. pmid:15731402
- 51. Zhou D, Shen Z, Strack AM, Marsh DJ, Shearman LP. Enhanced running wheel activity of both Mch1r- and Pmch-deficient mice. Regul Pept. 2005;124(1–3):53–63. pmid:15544841
- 52. Willie JT, Sinton CM, Maratos-Flier E, Yanagisawa M. Abnormal response of melanin-concentrating hormone deficient mice to fasting: Hyperactivity and rapid eye movement sleep suppression. Neuroscience. 2008;156(4):819–29. pmid:18809470
- 53. Mul JD, Yi C-X, van den Berg SAA, Ruiter M, Toonen PW, van der Elst MCJ, et al. Pmch expression during early development is critical for normal energy homeostasis. Am J Physiol Endocrinol Metab. 2010;298(3):E477–88. pmid:19934402
- 54. Nocedal I, Johnson AD. How transcription networks evolve and produce biological novelty. Cold Spring Harb Symp Quant Biol. 2015;LXXX. pmid:26657905
- 55. Villar D, Flicek P, Odom DT. Evolution of transcription factor binding in metazoans—mechanisms and functional implications. Nat Rev Genet. 2014 Apr;15(4):221–33. pmid:24590227
- 56. Sokolowski K, Esumi S, Hirata T, Kamal Y, Tran T, Lam A, et al. Specification of select hypothalamic circuits and innate behaviors by the embryonic patterning gene Dbx1. Neuron. 2015;86(2):1–14. pmid:25864637
- 57. Duman RS, Voleti B. Signaling pathways underlying the pathophysiology and treatment of depression: Novel mechanisms for rapid-acting agents. Trends Neurosci. 2012;35(1):47–56. pmid:22217452
- 58. Dias C, Feng J, Sun H, Shao NY, Mazei-Robison MS, Damez-Werno D, et al. β-catenin mediates stress resilience through Dicer1/microRNA regulation. Nature. 2014 Nov 12;516(7529):51–5. pmid:25383518
- 59. Lee JE, Wu S, Goering LM, Dorsky RI. Canonical Wnt signaling through Lef1 is required for hypothalamic neurogenesis. Development. 2006 Nov;133(22):4451–61. pmid:17050627
- 60. Ghanem N, Jarinova O, Amores A, Long Q, Hatch G, Park BK, et al. Regulatory roles of conserved intergenic domains in vertebrate Dlx bigene clusters. Genome Res. 2003 Apr;13(4):533–43. pmid:12670995
- 61. Pauls S, Geldmacher-Voss B, Campos-Ortega JA. A zebrafish histone variant H2A.F/Z and a transgenic H2A.F/Z:GFP fusion protein for in vivo studies of embryonic development. Dev Genes Evol. 2001 Dec;211(12):603–10. pmid:11819118
- 62. Berghmans S, Murphey RD, Wienholds E, Neuberg D, Kutok JL, Fletcher CDM, et al. tp53 mutant zebrafish develop malignant peripheral nerve sheath tumors. Proc Natl Acad Sci U S A. 2005 Jan 11;102(2):407–12. pmid:15630097
- 63. Johnson CW, Hernandez-Lagunas L, Feng W, Melvin VS, Williams T, Artinger KB. Vgll2a is required for neural crest cell survival during zebrafish craniofacial development. Dev Biol. 2011 Sep;357(1):269–81. pmid:21741961
- 64. Gil-Sanz C, Espinosa A, Fregoso SP, Bluske KK, Cunningham CL, Martinez-Garay I, et al. Lineage tracing using Cux2-Cre and Cux2-CreERT2 mice. Neuron. 2015;86(4):1091–9. pmid:25996136
- 65. Clapcote SJ, Roder JC. Simplex PCR assay for sex determination in mice. Biotechniques. 2005;38(5):702–6. pmid:15945368
- 66. Woo K, Fraser SE. Order and coherence in the fate map of the zebrafish nervous system. Development. 1995 Aug;121(8):2595–609. pmid:7671822
- 67. Eisenhoffer GT, Loftus PD, Yoshigi M, Otsuna H, Chien C-B, Morcos PA, et al. Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia. Nature. 2012 Apr 15;484(7395):546–9. pmid:22504183
- 68. Byars CL, Bates KL, Letsou A. The dorsal-open group gene raw is required for restricted DJNK signaling during closure. Development. 1999;126:4913–23. pmid:10518507
- 69. Thisse C, Thisse B. High-resolution in situ hybridization to whole-mount zebrafish embryos. Nat Protoc. 2008 Jan;3(1):59–69. pmid:18193022
- 70. Logel J, Dill D, Leonard S. Synthesis of cRNA probes from PCR-generated DNA. Biotechniques. 1992;13(4):604–606+608. pmid:1476730
- 71. Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL. Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics. 2012 Jan;13:134. pmid:22708584
- 72. Dorsky RI, Snyder A, Cretekos CJ, Grunwald DJ, Geisler R, Haffter P, et al. Maternal and embryonic expression of zebrafish lef1. Mech Dev. 1999;86(1–2):147–50. pmid:10446273
- 73. Wang X, Lee JE, Dorsky RI. Identification of Wnt-responsive cells in the zebrafish hypothalamus. Zebrafish. 2009;6(1):49–58. pmid:19374548
- 74. Stapleton M, Carlson J, Brokstein P, Yu C, Champe M, George R, et al. A Drosophila full-length cDNA resource. Genome Biol. 2002;3(12):RESEARCH0080. pmid:12537569
- 75. Oxtoby E, Jowett T. Cloning of the zebrafish krox-20 gene (krx-20) and its expression during hindbrain development. Nucleic Acids Res. 1993;21(5):1087–95. pmid:8464695
- 76. Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, et al. A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain. Neuron. 2014;83(2):309–23. pmid:24952961
- 77. Schaeren-Wiemers N, Gerfin-Moser A. A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes. Histochemistry. 1993 Dec;100(6):431–40. pmid:7512949
- 78. Hurst JL, West RS. Taming anxiety in laboratory mice. Nat Methods. 2010;7(10):825–6. pmid:20835246
- 79. Hånell A, Marklund N. Structured evaluation of rodent behavioral tests used in drug discovery research. Front Behav Neurosci. 2014;8(July):252. pmid:25100962
- 80. McLean AC, Valenzuela N, Fai S, Bennett SAL. Performing vaginal lavage, crystal violet staining, and vaginal cytological evaluation for mouse estrous cycle staging identification. J Vis Exp. 2012;(67):e4389. pmid:23007862
- 81. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protoc. 2008 Jun;3(6):1101–8. pmid:18546601
- 82. Spandidos A, Wang X, Wang H, Seed B. PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification. Nucleic Acids Res. 2010 Jan;38(Database issue):D792–9. pmid:19906719
- 83. Yu M, Xi Y, Pollack J, Debiais-Thibaud M, MacDonald RB, Ekker M. Activity of dlx5a/dlx6a regulatory elements during zebrafish GABAergic neuron development. Int J Dev Neurosci. 2011;29(7):681–91. pmid:21723936
- 84. Reed B, Jennings M. Guidance on the housing and care of Zebrafish Danio rerio. Res Anim Dep Sci Group, RSPCA, West Sussex, United Kingdom. 2010.