Advertisement
  • Loading metrics

Correction: Neuronal Hyperactivity Disturbs ATP Microgradients, Impairs Microglial Motility, and Reduces Phagocytic Receptor Expression Triggering Apoptosis/Microglial Phagocytosis Uncoupling

  • Oihane Abiega,
  • Sol Beccari,
  • Irune Diaz-Aparicio,
  • Agnes Nadjar,
  • Sophie Layé,
  • Quentin Leyrolle,
  • Diego Gómez-Nicola,
  • María Domercq,
  • Alberto Pérez-Samartín,
  • Víctor Sánchez-Zafra,
  • Iñaki Paris,
  • Jorge Valero,
  • Julie C. Savage,
  • Chin-Wai Hui,
  • Marie-Ève Tremblay,
  • Juan J. P. Deudero,
  • Amy L. Brewster,
  • Anne E. Anderson,
  • Laura Zaldumbide,
  • Lara Galbarriatu,
  • Ainhoa Marinas,
  • Maria dM. Vivanco,
  • Carlos Matute,
  • Mirjana Maletic-Savatic,
  • Juan M. Encinas,
  •  [ ... ],
  • Amanda Sierra
  • [ view all ]
  • [ view less ]
  • Article
  • Metrics
  • Comments
  • Media Coverage

The authors would like to report a typographical error that states the concentration of staurosporine used for the induction of apoptosis is 10 μM. The actual concentration used is 1 μM. The authors confirm that the changes do not affect the results of the experiments.

The typographical error can be found in the following two instances and are corrected here:

In the Figure 8 legend, Panel F (page 17 in the PDF):

“Primary cultures were pre-treated with KA (1 mM) for 2 h prior to adding apoptotic NE-4C cells (treated with 5 μM CM-DiI for 25 min and 1 μM staurosporine for 4 h).”

Under Materials and Methods, in the second paragraph under the ‘Primary Microglia Cultures’ heading (Page 32 in the PDF):

“NE-4C were previously labeled with the membrane marker CM-DiI (5 μM; 10 min at 37°C, 15 min at 4°C; Invitrogen) and treated with staurosporine (1 μM, 4h; Sigma) to induce apoptosis.”

Reference

  1. 1. Abiega O, Beccari S, Diaz-Aparicio I, Nadjar A, Layé S, Leyrolle Q, et al. (2016) Neuronal Hyperactivity Disturbs ATP Microgradients, Impairs Microglial Motility, and Reduces Phagocytic Receptor Expression Triggering Apoptosis/Microglial Phagocytosis Uncoupling. PLoS Biol 14(5): e1002466. pmid:27228556