Plasmodesmata play a key role in leaf vein patterning

Leaf veins provide a vital transport route in plants. The formation of leaf vein patterns has fascinated many scientists. In PLOS Biology, Linh and Scarpella reveal that transport through plasmodesmata plays a key role in vein patterning.

and temporal variations in plasmodesmal permeabilities during vein development. By inducing and observing fluorescent proteins, they revealed that plasmodesmal permeability is high throughout the leaf at early stages; however, as the veins form, plasmodesmal diffusion reduces between the veins and non-vasculature tissue. Once developed, the veins are symplastically isolated, so that small molecules can diffuse through plasmodesmata between adjacent vein cells but cannot diffuse into the adjacent non-vein cells (Fig 1). These spatial differences in plasmodesmal diffusion were found to be essential for correct vein patterning.
Linh and Scarpella then considered whether plasmodesmal diffusion contributed to vein induction after auxin application and how this process co-ordinates with the established roles of auxin transport and signalling (described in [2][3][4]). Using the callose synthesis mutants (in which spatial variations in plasmodesmal permeability did not occur), auxin application induced veins in significantly fewer cases. Furthermore, applying auxin to wild-type leaves delayed the reduction in plasmodesmal permeability between the veins and non-vasculature tissue, suggesting a coupling between auxin levels and plasmodesmal fluxes.
The coupling between auxin and plasmodesmata was further investigated using chemical treatments: inhibiting polar auxin transport (by applying NPA) delayed the reduction in plasmodesmal permeability between veins and non-vasculature tissue, whereas inhibiting auxinsignalling (by applying PBA), promoted the restriction in plasmodesmal permeability. Thus, the regulation of plasmodesmal permeability depends on both polar auxin transport and auxin signalling. Furthermore, in the callose synthesis mutants, the connected and continuous PIN1 domains characteristic of vein patterning become gradually disconnected (due to loss of PIN1 expression in segments of the initial PIN1 domains), suggesting feedbacks between plasmodesmal and PIN1-mediated auxin transport.
Having established a crucial role for plasmodesmal diffusion in vein patterning, Linh and Scarpella questioned whether this process is regulated by GNOM (GN), which is a regulator of membrane trafficking and was shown to regulate an undiscovered component of vein patterning in [4]. They observed that regulation of plasmodesmal permeability is defective in gn mutants, and that simultaneous inhibition of the regulation of plasmodesmal permeability, auxin transport, and auxin signalling phenocopies the strong gn mutant. They concluded that GNOM (GN) forms a signalling hub controlling the 3 components of vein formation: auxin signalling, polar transport, and diffusion of an auxin signal through plasmodesmata.
Precisely how the plasmodesmal permeability is regulated during vein formation remains an open question. While the observations using callose synthesis mutants suggest that callose reduces plasmodesmal permeability, the data presented does not ruled out the possibility that differences in plasmodesmal density or structure also contribute to the observed spatial variations in plasmodesmal diffusion. Furthermore, the molecular details as to how auxin is regulating plasmodesmal permeabilities remain uncovered. Key to previous studies on phototropism and lateral root emergence [6,8] was the observation that auxin increases callose levels to restrict its own movement. In contrast, a study of leaf hyponasty found that the effective plasmodesmal permeability was not affected by exogeneous auxin treatments [7]. However, this new study on vein patterning provides a further scenario, with plasmodesmal diffusion being high between 2 adjacent vein cells with high auxin, but low between a high-auxin vein cell and adjacent low-auxin non-vasculature cell. Precisely how auxin regulates these plasmodesmal permeabilities, how GNOM contributes to this regulation, and how the plasmodesmal regulation combines with auxin's regulation of PIN1 levels [9] remain intriguing avenues for future research.
In summary, Linh and Scarpella [5] elucidate a key mechanistic component of Sach's Canalisation hypothesis. Their elegant study demonstrates that regulation of plasmodesmata is essential for vein patterning. They conclude that vein patterning occurs through the co-ordinated action of auxin signalling, polar transport, and diffusion of an auxin signal through plasmodesmata, 3 processes that are all regulated by GNOM. How these findings translate to either other plant patterning processes or vein patterning in other species [10] form open and exciting questions for future research.  [5] show that in addition to polarised PIN1-mediated auxin transport within vein cell files, open plasmodesmata enable diffusion between adjacent vein cells. Their study reveals that diffusion of an auxin signal through plasmodesmata is essential to leaf vein patterning in Arabidopsis. https://doi.org/10.1371/journal.pbio.3001806.g001