Def1 Promotes the Degradation of Pol3 for Polymerase Exchange to Occur During DNA-Damage–Induced Mutagenesis in Saccharomyces cerevisiae

After DNA damage, Def1 triggers degradation of the catalytic subunit of the replicative DNA polymerase at stalled replication forks, allowing special polymerases to take over DNA synthesis.


Introduction
The stalling of the replication machinery that occurs as a consequence of encountering unrepaired DNA damages is a challenging problem for cells. Stalled replication forks can undergo DNA breakage and recombination that can lead to chromosomal rearrangements and cell death. To ensure survival, cells have evolved different mechanisms that can sustain DNA replication on damaged templates. These so-called DNA damage tolerance or DNA damage bypass processes allow replication to continue on damaged DNA without actually removing the damage. DNA damage tolerance is achieved through two main mechanisms: template switching and translesion synthesis (TLS). Template switching is inherently error-free, as replication continues by using the undamaged nascent sister chromatid as a template for the bypass of the lesion [1], whereas during TLS, specialized polymerases take over the nascent primer end from the replicative polymerase and carry out synthesis opposite the DNA lesion in an error-free or error-prone way [2].
Rad6 and Rad18 are key mediators of DNA damage tolerance in the yeast Saccharomyces cerevisiae [3,4]. They govern at least three different pathways for the replication of ultraviolet light (UV)damaged DNA: (1) Rad5-dependent error-free DNA damage bypass, (2) Rad30-dependent error-free TLS, and (3) Rev3-dependent error-prone TLS. Rad6 is an ubiquitin conjugase [5], and it forms a complex in the cell with Rad18, a RING finger ATP-ase with single-stranded DNA-binding activity [6]. Upon UV-treatment, the Rad6-Rad18 ubiquitin-conjugase-ligase complex monoubiquitylates proliferating cell nuclear antigen (PCNA), the essential processivity factor for replicative DNA polymerases, at its lysine-164 residue at the stalled replication fork [7]. Monoubiquitylated PCNA activates the Rev3, and the Rad30dependent subpathways involving TLS polymerases, whereas further polyubiquitylation of PCNA on the same residue through a lysine-63-linked chain by the Rad5-Mms2-Ubc13 ubiquitinconjugase-ligase complex activates the Rad5 subpathway [7,8]. Genetic experiments suggest that the Rad5 branch operates through template switching, where the newly synthesized strand of the undamaged sister duplex serves as a template to bypass the lesion [9]. Rad5, a SWI-SNF family member helicase, most probably directly promotes this process through its fork-reversal activity [10]. The RAD30-encoded DNA polymerase g (Polg) is unique in its ability to efficiently and accurately synthesize through UV-induced cyclobutane pyrimidine dimers [11]. In accordance with its role in the error-free bypass of UV lesions, a defect of Polg in yeast confers an increase in UV-induced mutations, and in humans it causes the cancer-prone syndrome, the variant form of xeroderma pigmentosum [12][13][14]. Besides UV-lesions, Polg can bypass several DNA distorting lesions with varying accuracy [2]. The mutagenic branch involves Rev1 and Rev7, besides Rev3, and the lack of either protein causes immutability [15]. The Rev1 protein is a DNA polymerase with limited ability to insert C residues [16]. Its catalytic activity is dispensable for most induced mutagenesis events, suggesting a mainly structural role for Rev1 [17]. Rev3 together with Rev7 forms DNA polymerase f (Polf) [18]. Rev7 is an accessory protein, whereas Rev3 is the catalytic subunit. Polf has the ability to efficiently extend from mispaired nucleotides and from nucleotides inserted opposite different DNA lesions [2].
TLS polymerases synthesize DNA with a high error rate and are responsible for introducing mutations into the genome during DNA damage bypass, so their replacement of the replicative polymerase must be tightly regulated. However, our understanding of the polymerase switch at DNA damage sites is elusive. Polg, Rev1, and Polf were shown to interact with PCNA, and it was suggested that through these interactions TLS polymerases could get access to the replication fork [19][20][21]. Also, the interaction with PCNA was shown to be essential for the in vivo function of all three polymerases. Though PCNA binding can give access to TLS polymerases to the replication fork, the mechanism that allows them to actually take over DNA synthesis from the replicative polymerase during DNA lesion bypass is still unknown.
In this study, we identify Def1 as an indispensable regulator of induced mutagenesis. We show that Def1 promotes the ubiquitylation and subsequent proteasomal degradation of the catalytic subunit of the replicative polymerase after DNA damage treatment. We demonstrate that the noncatalytic subunits of the replicative polymerase are not affected by UV-induced degradation and that they can form a complex with the TLS polymerase Rev1. Based on our results we propose a new model for polymerase exchange at stalled replication forks.

Rad5 Forms a Stable Complex with Def1 upon MMS Treatment
In searching for new factors affecting DNA damage tolerance, we aimed to identify new interacting partners of Rad5. Therefore, we performed tandem affinity purification (TAP) of Rad5 together with its complexes. For that purpose, we introduced a TAP tag, consisting of a calmoduline binding peptide and two IgG binding domains of protein A separated by a TEV protease cleavage site, at the C-terminus of Rad5 at the chromosomal locus. We purified Author Summary DNA damages can lead to the stalling of the cellular replication machinery if not repaired on time, inducing DNA strand breaks, recombination that can result in gross chromosomal rearrangements, even cell death. In order to guard against this outcome, cells have evolved several precautionary mechanisms. One of these involves the activity of special DNA polymerases-known as translesion synthesis (TLS) polymerases. In contrast to the replicative polymerases responsible for faithfully duplicating the genome, these can carry out DNA synthesis even on a damaged template. For that to occur, they have to take over synthesis from the replicative polymerase that is stalled at a DNA lesion. Although this mechanism allows DNA synthesis to proceed, TLS polymerases work with a high error rate even on undamaged DNA, leading to alterations of the original sequence that can result in cancer. Consequently, the exchange between replicative and special polymerases has to be highly regulated, and the details of this are largely unknown. Here we identified Def1-a protein involved in the degradation of RNA polymerase II-as a prerequisite for error-prone DNA synthesis in yeast. We showed that after treating the cells with a DNA damaging agent, Def1 promoted the degradation of the catalytic subunit of the replicative DNA polymerase d, without affecting the other two subunits of the polymerase. Our data suggest that the special polymerases can take over synthesis only after the catalytic subunit of the replicative polymerase is removed from the stalled fork in a regulated manner. We predict that the other two subunits remain at the fork and participate in TLS together with the special polymerases. Rad5 and its interacting partners through the two affinity tags under native conditions. To facilitate the formation of damage bypass complexes, we applied 0.02% methyl methanesulfonate (MMS) for 2 h before collecting the cells. Surprisingly, without treatment only one prominent, specific band was visible in the final, highly purified fraction on the Coomassie-stained gel (Figure 1), which was identified by mass spectrometry as the tagged Rad5 itself. However, after MMS treatment two prominent bands appeared on the gel; the lower mobility band was again Rad5, whereas the higher mobility band was identified as Def1. Repetition of the experiment yielded the same result that Def1 copurified with Rad5, but only after treating the cells with MMS. It suggested that either DNA-damage-induced posttranslational modification of Rad5 and/or Def1 or a third, damage-specific factor was necessary to promote the formation of the complex.

DEF1 Functions in the RAD6-Mediated DNA Damage Tolerance
Rad5 mediates an error-free DNA damage tolerance pathway under the control of Rad6-Rad18, whereas Def1 has a role in promoting the proteolytic degradation of stalled RNA polymerase II and in telomere maintenance [22,23]. To further establish a connection between Rad5 and Def1, we analyzed the genetic relations between DEF1 and RAD5, and members of all three branches of the RAD6-governed pathway upon DNA damage. After treating the cells with UV, the sensitivity of the def1 rad6 double deletion strain did not exceed that of the rad6 mutant, pointing to an epistatic relationship between DEF1 and RAD6 ( Figure 2A). The higher resistance seen with the double mutant might originate from other functions of these multitask proteins, as the def1 rad18 strain showed the same sensitivity as rad18 (unpublished data), fortifying the involvement of DEF1 in the RAD6-dependent DNA damage tolerance. Surprisingly, the def1 rad5 double deletion strain displayed much higher sensitivity than any of the corresponding single mutants, indicating that DEF1 acted outside of the RAD5-dependent subpathway (Figures 2B). This was verified by the hypersensitivity of the def1 mms2 strain over the single mutants ( Figure 2C). Also, the def1 rad30 double mutant was more sensitive to UV than either def1 or rad30, implying that DEF1 functioned independently of RAD30 ( Figure 2D). Nevertheless, the def1 rev3 strain exhibited the same sensitivity as the def1 mutant, which indicated an epistatic relationship between DEF1 and REV3 ( Figure 2E). We carried out similar experiments using MMS instead of UV as a DNA damage source (Figures 2F-J). Upon MMS treatment DEF1 showed epistasis with RAD6 and REV3, but its deletion further sensitised rad5 and mms2, proving again the involvement of DEF1 in the mutagenic branch of the RAD6-governed DNA damage tolerance. However, DEF1 also showed epistasis with RAD30, as the double mutant was as sensitive as the def1 single mutant. We note that this reflected a real epistatic relationship, as although rad30 itself was not sensitive to MMS, only at very high doses, it was hypersensitive with mms2, but also showed epistasis with REV3 ( Figure 2K,L). That means that in the bypass of MMS-induced DNA lesions, RAD30 works together with the members of the REV3 branch. In conclusion, our data strongly suggested that DEF1 participated in the REV3-dependent mutagenic branch of the RAD6-RAD18-regulated DNA damage tolerance.

DNA-Damage-Induced Mutagenesis Is Abolished in def1 Deletion Mutants
The TLS polymerases of the REV3 branch are responsible for virtually all damage-induced mutagenesis; consequently, inactivation of either one causes a strong antimutator effect [15]. To prove that DEF1 belonged to the REV3 branch, we measured the rate of UV-induced mutations in def1 strains. In keeping with the results of the epistasis analysis, induced mutagenesis was completely abolished in def1 (Figure 3). In fact, def1 was even more defective than the rev3 strain. Additional deletion of DEF1 in mms2 also eliminated induced mutagenesis, though mms2 by itself causes high mutagenesis, most probably because in the absence of the error-free branch, lesions are channelled to the REV3-dependent mutagenic pathway. Ectopic expression of Def1 in def1 cells restored close to wild-type-level mutagenesis, confirming that the immutability was in fact due to the absence of DEF1. We obtained the same results using MMS instead of UV (unpublished data). From these we concluded that DEF1 played an essential role in induced mutagenesis. For the REV3 branch to operate, the TLS polymerases of the branch have to take over synthesis from the replicative polymerase stalled at a DNA lesion site, a central but poorly understood step in DNA lesion bypass. Because Def1, unlike other members of the REV3 branch, is not a DNA polymerase, we surmised that it might facilitate the exchange between the TLS and the replicative polymerases. As Def1 played a role in the ubiquitylation of stalled RNA polymerase II [22], we considered the possibility that, similarly, it could mediate ubiquitylation of the stalled replicative DNA polymerase. Ubiquitylation then could lead to polymerase switch by either playing a regulatory role as in the case of DNAdamage-induced ubiquitylation of PCNA [7], or it could result in protein removal through degradation. To test these possibilities, we followed the fate of the replicative polymerase during DNA damage bypass by monitoring Pol3, the catalytic subunit of the replicative DNA polymerase d (Pold) during cell cycle in UVtreated, synchronized yeast cultures. In order to facilitate TLS, we first used an mms2 deletion strain. Importantly, we observed a transient decrease in the level of Pol3 upon UV irradiation as opposed to normal growth conditions, and the degree of degradation correlated with the applied UV doses (Figure 4). We could also detect degradation in wild-type cells in the S phase of the cell cycle, as indicated by the expression pattern of the G2/ M-specific cyclin Clb2 ( Figure 5A). However, in experiments using a def1 deletion strain, we could not detect any decrease in the level of Pol3 ( Figure 5B). To investigate whether the observed phenomenon was ultimately under the higher control of RAD6, we performed the same experiment in a rad6 strain and found that Pol3 diminution was also absent ( Figure 5C). On the other hand, reduction of Pol3 could be seen in mms2 ( Figure 5D) and in rad30 ( Figure 5E) backgrounds. These results, in conjunction with the above genetic results, strongly implied that Pol3 diminution was specifically dependent on DEF1, which exerted this function under the control of RAD6, in the mutagenic DNA damage bypass pathway.

Def1 Induces the Ubiquitylation and Proteasomal Degradation of Pol3
The most plausible explanation for the transient decrease of Pol3 would be that Pol3 underwent regulated protein degradation induced by UV. The majority of regulated proteolysis takes place in the proteasome in eukaryotic cells. To resolve whether the decrease in the Pol3 protein level was due to protein degradation mediated by the proteasome, we supplemented the growth media with the proteasome inhibitor MG132. Indeed, in the presence of MG132, the UV-induced degradation of Pol3 could not be observed ( Figure 5D). To add further evidence, we applied a temperature-sensitive rpn7 mutant displaying defects in proteasome function at high temperature (37uC) but behaving like wildtype at low temperature (25uC) [24]. Using this mutant we could not detect degradation at the restrictive high temperature contrary to the permissive low temperature ( Figure 6A), whereas in the RPN7 strain degradation occurred at both temperatures (unpublished data). These results demonstrated that the proteasome was responsible for the UV-induced degradation of Pol3.
Ubiquitylation is a major signal for proteasomal protein degradation. To show ubiquitylation of Pol3, N-terminally 7 histidine-tagged ubiquitin was expressed in yeast cells and ubiquitylated proteins from cell extracts prepared after irradiating cells with UV were enriched on nickel beads. Indeed, we could

Pol31 and Pol32 Are Not Subject to UV-Induced Degradation
Pold is a heterotrimer and consists of two noncatalytic subunits, Pol31 and Pol32, besides Pol3 [25]. Pol31, like Pol3, is essential for cell viability, but Pol32 is a nonessential subunit. Pol3 forms a stable complex with Pol31, and Pol32 is attached to this complex through its interaction with Pol31 [26]. We aimed to examine whether the whole Pold enzyme was subject to UV-induced proteolysis, or it affected only the catalytic subunit. We found that contrary to Pol3, Pol31 and Pol32 were not affected by UVinduced degradation ( Figure 5F and 5G).

Pol31 and Pol32 Can Form a Complex with Rev1
Taken together, these results suggested that during DNA damage bypass, Pol31 and Pol32 remained at the stalled fork. We postulated that a TLS polymerase could take the place of Pol3 and carry out lesion bypass in complex with Pol31 and Pol32. To test this idea, we examined whether Pol31 and Pol32 together could form a complex with Rev1 in in vitro assays using purified proteins. We chose Rev1, because it had been suggested to function as a scaffold in TLS, based on its interaction in yeast with Polg and Polf [27,28], and in mouse and human cells with Polg, Poli, and Polk [29,30]. Also, it has already been shown to interact with Pol32 [31]. In GST pull-down assays we added Pol31 and Rev1 to GST-Pol32 immobilised on glutathione-Sepharose affinity beads, and after incubation bound proteins were released from the beads by glutathione. As shown in Figure 7B lanes 1-4, both Pol31 and Rev1 eluted together with GST-Pol32, indicating that these proteins formed a complex together. In control experiments using GST instead of GST-Pol32, only GST was present in the elution fraction, confirming that the interaction between Pol31, Pol32, and Rev1 was specific ( Figure 7B, lanes 5-8). In conclusion, purified Pol31, Pol32, and Rev1 could interact directly and form a stable multisubunit protein complex.

Discussion
In this study we identified a DNA-damage-induced complex of Rad5 with Def1. Our genetic studies placed DEF1 in the RAD6-RAD18-dependent DNA damage tolerance pathway, where it played an indispensible role during induced mutagenesis. We established that Pol3, the catalytic subunit of the replicative DNA polymerase Pold, was degraded upon UV irradiation. We presented evidence that degradation of Pol3 was the result of polyubiqitylation-mediated proteasomal degradation, and it was dependent on DEF1 under the higher control of RAD6. Conversely, Pol31 and Pol32, the other two subunits of Pold, were not degraded. We also demonstrated that Pol31 and Pol32 together could form a stable complex with the TLS polymerase Rev1. Based on these results, we propose a new model for polymerase exchange at stalled replication forks (Figure 8). During replication, when Pold stalls at a DNA lesion, PCNA gets ubiquitylated by Rad6-Rad18. Monoubiquitylated PCNA acti- Figure 6. UV-induced degradation of Pol3 is mediated by the proteasome and is triggered by its Def1-dependent polyubiquitylation. (A) The rpn7-3 mutant is deficient in degrading Pol3. Experiments were done as described in Figure 5, except that for the inactivation of the proteasome, cells were shifted to 37uC 2 h before a factor treatment, and after 3 h of synchronization, irradiated with 200 J/m 2 . Anti-HA antibody was used to detect HA-tagged Pol3. Cell cycle progression was monitored by Clb2 cyclin levels, and PGK served as a loading control. The level of Pol3 relative to PGK is shown at the bottom of each panel. (B) Def1 assists Pol3 polyubiquitylation. Polyubiquitylated proteins from cell extracts prepared from 100 ml of cell culture before and 20 and 30 min after UV irradiation were bound to NiNTA agarose (Qiagen), and Pol3 was identified in the bound fraction with HA antibody (upper panels). The PGK and Pol3 levels in the extracts before adding to the beads are also shown, and the bound fractions were probed with anti-ubiquitin antibody (lower panels vates TLS, for which to occur Pol3 is ubiquitylated by a Def1dependent manner and removed from the stalled Pold complex through proteasomal degradation. We hypothesize that a TLS polymerase takes over the place of Pol3 and teams up with the remaining Pold subunits, Pol31 and Pol32, at the stalled fork to form a new complex capable of executing DNA lesion bypass, suggested by our results showing complex formation of Rev1-Pol31-Pol32, and also by recent studies detecting complex formation of yeast Pol31-Pol32-Rev3-Rev7 and also of their human counterparts [32][33][34]. We surmise that after lesion bypass and deubiquitylation of PCNA, the TLS polymerase is removed from the primer terminus, Pol3 restores Pold by regaining its place, and replication continues. Importantly, this model gives an explanation for previous genetic results showing that in pol32 cells induced mutagenesis is severely impaired, and that deletion of the N-terminal part of Pol32, responsible for binding Pol31, also abolishes induced mutagenesis [25,35,36].
Our data raise an important question: How can the RAD30encoded TLS polymerase, Polg, operate independently of Def1? Our results imply that Pol3 does not have to be removed from the stalled fork for Polg-dependent UV-lesion bypass to occur. Polg is mainly specialized for the error-free bypass of UV lesions, so it is reasonable to assume that Polg should have preference over the error-prone TLS polymerases in the bypass of UV-induced DNA damages. Polg differs from the other TLS polymerases, Rev1 and Polf, in its way of binding PCNA. Although Rev1 and Polf bind the intermolecular interface at the outer face of the PCNA ring [20,21], Polg, similarly to Pold, binds the interdomain connector loop of PCNA through its conserved PCNA-interacting peptide motif [19]. Given that PCNA is a homotrimer ring, Pold and Polg could bind the same PCNA ring simultaneously. We presume that transient conformational changes, probably induced by the stalling of the fork and ubiquitylation of PCNA, could allow Polg to take over synthesis from Pold, as also suggested by in vitro experiments [37], while both remain attached to PCNA. Because Polg synthesizes opposite pyrimidine dimers with the same kinetics as it does opposite undamaged DNA [38], rapid bypass can occur. Deubiquitylation of PCNA would restore the original conformation and Pold could continue synthesis. We note that this is in accord with the in vivo finding that Pol32 is not needed for TT dimer bypass carried out by Polg [39]. On the other hand, when the damage poses a kinetic barrier also to the TLS polymerase, for the slower kinetic damage bypass to occur, Pol3 has to be removed so that the TLS polymerases could form a stable complex with Pol31 and Pol32. This would also explain the epistasis of RAD30 with DEF1 in the bypass of MMS-induced DNA lesions, as the efficiency of incorporation by Polg is reduced ,20-fold opposite 6 O-methylguanine and ,1,000-fold opposite an abasic site [40,41].
We detected a very stable DNA-damage-induced complex formation of Rad5 with Def1. However, our genetic data placed the two genes into two alternative DNA damage tolerance pathways, both governed by Rad6. We hypothesize that the Def1-Rad5 complex might coordinate the activity of the two subpathways in response to DNA damage. A similar role of Def1 during transcription was suggested, where Def1 assisted in the degradation of the RNA polymerase stalled at DNA damage sites, and probably coordinated the repair mechanisms through its interaction with Rad26 [22].
The high conservation between elements of DNA lesion bypass from yeasts to humans, including the Rad6-Rad18 and Rad5-Mms2-Ubc13 complexes and their enzymatic activities, the TLS polymerases, and PCNA ubiquitylation [42], suggests that DNAdamage-induced selective degradation of the catalytic subunit of the replicative DNA polymerase drives polymerase exchange in higher eukaryotes as well. The role of TLS polymerases in mutagenesis and in cancer makes it highly important to uncover further details of polymerase exchange, to identify and investigate further factors that affect Pol3 degradation, and to check the existence of a similar mechanism in human cells.

Sensitivity Assays
For qualitative analysis of sensitivity to MMS, cells were serial diluted and spotted onto YPD plates containing defined amounts of MMS and grown at 30uC for 3-5 d. For quantification, cells were spread onto YPD plates at appropriate dilutions and irradiated with UV light (254 nm) for varying times to apply the specified dosage. Plates were incubated in the dark at 30uC, and colonies were counted after 3-5 d.

UV-Induced Mutation Rate
UV-induced forward mutation frequencies at the CAN1 locus were measured by comparing the numbers of can1 R colonies at given UV doses, selected on synthetic complete medium without arginine and containing canavanine, with the numbers of survivors on complete synthetic medium, exposed to the same UV doses.

Cell Synchronisation
Logarithmically growing cells in YPD at 30uC were arrested at A 600 :0.8 in G1 by 100 ng/ml a-factor (Sigma) for 2-4 h, washed, resuspended in phosphate buffered saline, and divided into Petri dishes for UV irradiation. Half of the cultures were irradiated with the given UV dose, and the other half served as untreated control. Cells were released back into growth medium containing 50 mg/ ml pronase (Calbiochem) to inactivate any residual a-factor. For experiments showing polyubiquitylation of Pol3, the growth media always contained 100 mM CuSO 4 to induce 7His-ubiquitin expression. Samples were taken at given time points after UV treatment for whole cell extract preparation. Experiments involving MG132 (Sigma) were done in pdr5 background. MG132 (50 mM) was added to the a-factor synchronized cultures 1 h before UV irradiation. The rpn7-3 mutant and its isogenic wild-type strain were grown at 25uC. To detect the mutant phenotype we followed the protocol described in [24]. Briefly, 50 ml culture of logarithmically growing cells (A 600 :0.5) were split. Half of the culture was kept at 25uC and the other half was shifted to 37uC. At A 600 :0.8 cultures were synchronised by a-factor for 3 h and processed as detailed above.

Protein Techniques and Antibodies
Whole cell extracts were prepared according to a trichloroacetic acid (TCA) protein precipitation method [43] except that after TCA precipitation, pellets were washed with ice-cold acetone, air-dried, and resuspended in 16 Laemmli sample buffer before loading to an 8% poly-acrylamide gel. Polyubiquitylated Pol3 was detected using denaturing NiNTA chromatography as described in [49]. Antibodies against HA (Gene Tex), Clb2 (Santa Cruz), PGK (Molecular Probes), and ubiquitin (Santa Cruz) were used. Pol31, Pol32, and Rev1 were overexpressed in Nterminal fusion with GST and purified on glutathione-Sepharose 4B beads following the protocol in [45], with the exception that in the case of Rev1, 0.1% Triton X-100 was added to the lysate after breaking the cells. In the case of Pol31 and Rev1, the GST tag was removed by PreScission protease cleavage in the elution step of purification. For complex formation, GST-Pol32 (3 mg) immobilized on glutathione-Sepharose beads was incubated with purified Pol31 (5 mg) and Rev1 (3 mg), overnight on ice in buffer containing 50 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 10% glycerol, 0.01% Nonidet P-40. Beads were washed five times with the same buffer, and bound proteins were eluted in the same buffer containing 20 mM reduced glutathione. Various fractions were analyzed by SDS/ PAGE.

TAP
Four liters of yeast culture were grown to logarithmic phase in synthetic complete medium, and at A 600 :0.8, half of the culture was treated with 0.02% MMS for 2 h before harvesting. TAP purification was carried out as described [44] with the following modifications: cells were broken in 16 YBB (50 mM Tris/HCl pH:7.5, 50 mM KCl, 100 mM NaCl, 10% sucrose) supplemented with protease inhibitors. After clarifying the lysate with ultracentrifugation for 1 h with 100,000 g, 2-mercaptoethanol was added to 8.5 mM, Nonidet P-40 to 0.01%, and NaCl to 500 mM final concentration, and the lysate was transferred into an IgG Sepharose bead (Amersham)-filled column. In later steps the protocol was followed. Briefly, bound fraction was eluted with TEV protease cleavage. The elution fraction was applied on calmodulin beads, and bound proteins were recovered by eluting in EGTA-containing buffer. Proteins were concentrated and analysed on a 6%-12% gradient sodium dodecyl sulphate polyacrylamide gel stained with Coomassie blue R-250. Excised protein bands were identified by MALDI-TOF mass spectrometry after trypsin digestion. Eleven peptides of the higher mobility band matched yeast Def1 (55% coverage) and they covered 18% of the DEF1 sequence.