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Cell-level metadata are indispensable for documenting single-cell sequencing datasets

Fig 1

Processed data files necessary for replicating single-cell studies.

(A) Example of a gene-by-cell count matrix containing single-cell measurements and a cell-level metadata table containing annotations inferred from the analysis of the single-cell dataset. (B) Workflow of analysis steps for regenerating cell type or gene-expression signatures from public datasets for comparative analysis of single-cell datasets. * indicates a step requiring an analyst to make subjective decisions; ** indicates a step that often includes a nondeterministic algorithm.

Fig 1