A 3D-printed hand-powered centrifuge for molecular biology
Fig 3
Analysis of chromoprotein expression in high-school.
(A) Location of high school on map of the United States. (B) Workflow schematic for color analysis, beginning with transformation of the plasmid and plating, growth of liquid cultures, isolation of small portion of culture, centrifugation with 3D-Fuge, and sample illumination chamber and color picker for measurements. (C) Images of 3D-Fuges utilized in experiments. (D) Image of sample illumination chamber and phone used for capturing the image (Left). Image of view of chromoprotein-expressing bacterial pellets in sample illumination chamber (Right). This image does not represent the pellets utilized for measurements indicated in Fig 3G. (E) Diagram for regulation of chromoprotein expression in plasmid construct. (F) Color wheel utilized for hue value measurements. As darker shades occur counterclockwise around the color wheel, darker colors correlate with a smaller hue value. (G) Hue value measurements for Scrooge Orange chromoprotein at varying IPTG concentrations. As expected, increasing IPTG concentrations results in smaller hue values, indicating successful analysis of color expression using the 3D-Fuge and sample illumination chamber. SD, N = 3 technical replicates. Data for the graph can be found on GitHub (https://github.com/bhamla-lab/3D-fuge-PlOS-Biology-2019) in the file named Data File for 3D-Fuge Figures (2) under the sheet titled “Fig 3G.” IPTG, Isopropyl β-D-1-thiogalactopyranoside.