A 3D-printed hand-powered centrifuge for molecular biology
Fig 2
Overview of steps involved with sample collection, use of the 3D-Fuge for nucleotide extractions, and DNA sequencing in the Amazon rainforest.
(A, B) Field site for the portable lab study located in Tamboapta, Peru. (C–F) Examples of specimens collected for in situ DNA sequencing, including (C) a butterfly, (D) a bloodfed mosquito, (E) a plant leaf, and (F) unknown insect eggs. (G, H) Tissue from specimens were homogenized and incubated in proteinase K and (I) the hand-powered 3D-Fuge was then used to perform DNA extraction and purification. (J) Purified DNA was subsequently used for downstream molecular steps including long-range PCR and real-time DNA sequencing on the MinION connected to a laptop. PCR, polymerase chain reaction.