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Microscopy Image Browser: A Platform for Segmentation and Analysis of Multidimensional Datasets

Fig 4

Quantification and visualization of results are the final steps of the imaging workflow.

Generated models may be quantified to extract different parameters of the segmented objects and visualized using a number of programs. As an example, the volumes (μm3) and numbers of segmented mitochondria were calculated (the plot in red). The quantifications results can either be plotted directly in MIB or exported to MATLAB or Microsoft Excel. The manual measurements of angles, distances, caliper, and radius complement automatic quantification. The visualization of the mitochondria model (in green) is demonstrated using six alternative programs (the lower row).

Fig 4