Dally Proteoglycan Mediates the Autonomous and Nonautonomous Effects on Tissue Growth Caused by Activation of the PI3K and TOR Pathways
(A–D) Cuticle preparations (A, C) and histograms plotting tissue size normalized as a percent of the control wings (B, D) of nub>Rheb (A, B) or nub>Dp110 (C, D) adult wings coexpressing either GFP or dallyRNAi. Two independent RNA interference (RNAi) lines were used in B. Error bars show the standard deviation. Number of wings analyzed per genotype ≥ 10. ***p < 0.001. (E) Representative wing discs of the indicated genotypes labeled to visualize dally-lacZ (antibody to β-Gal, green or white) and myrT expression (in red) to mark the transgene-expressing domain. Higher magnification pictures of the squared regions are shown on the right side. Note the reduced expression of dally-lacZ upon depletion of the TSC/TOR or PTEN/PI3K pathways. (F) Representative wing discs of the indicated genotypes and labeled to visualize Ci (red) and DAPI (in blue). Ci labels the anterior compartment. A, anterior compartment; P, posterior compartment. (G) Histogram plotting the P/A size ratio of wing discs of the indicated genotypes. Error bars show the standard deviation. Number of wing discs analyzed per genotype ≥ 10. ***p < 0.001. P/A ratios: hh>2XGFP = 0.55 ± 0.02; hh>GFP, Dp110-RNAi = 0.29 ± 0.06; hh>dally, Dp110-RNAi = 0.42 ± 0.07. (H) Histogram plotting absolute size in a.u. of adult wings of the indicated genotypes. Quantification was made in well-fed (100 g/L yeast food) and starved (20 g/L yeast food) animals. Error bars show the standard deviation. Number of wings analyzed per genotype ≥ 10. ***p < 0.001. ns, not significant.