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Luminal Progenitors Restrict Their Lineage Potential during Mammary Gland Development

Fig 1

Notch1 is expressed in multipotent embryonic stem cells.

(A) Schematic representation of the Notch1-CreERT2SAT knock-in mice (referred to as N1CreERT2) and Rosa26mTomato/mGFP reporter mice (called R26mTmG) used in this study. Pregnant females were induced with tamoxifen to label their embryos at embryonic day E15.5 and double transgenic N1CreERT2R26mTmG littermates were analyzed 24 h later (B–D) or 5 wk after birth (E–G). (B–D) Representative embryonic mammary bud sections show that Notch1pos cells (marked by GFP in green) express both myoepithelial (K5, in red in B) and luminal markers (K8, in red in C) and they are negative for ERα (in red in D), n = 2. (E–G) Representative pubertal mammary gland sections show that Notch1-derived clones (in green) contain myoepithelial (K5pos in red in E) and luminal cells (K8pos in red in F) as well as ERαpos and ERαneg cells (ERα in red in G), n = 3. 4',6-diamidino-2-phenylindole (DAPI) stains DNA in blue. Scale bars correspond to 20 µm in B–G, and 10 µm in the magnifications of the insets.

Fig 1