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Facilitation of AMPA Receptor Synaptic Delivery as a Molecular Mechanism for Cognitive Enhancement

Figure 7

FGL-triggered persistent activation of signaling pathways.

(A) Left: Western blot of hippocampal extracts treated with TPA (12-O-tetradecanoylphorbol-13-acetate; PKC activator that served as a positive control), untreated (“0”), and treated with FGL at different time-points after FGL application. The primary antibody detects phosphorylation of endogenous proteins at PKC substrate motifs (phospho-(Ser) PKC substrate). Right: Quantification of Western blots similar to the one shown on the left, by calculating the combined intensity from all bands in each lane. N, number of independent experiments. The p values were determined with the Mann-Whitney test. (B, C) Left: Western blot of hippocampal extracts treated with FGL at different time-points after FGL application and untreated (“0”). The primary antibodies detected phosphorylated CaMKII at Thr286 (p-CaMKII) and total levels of CaMKII (T-CaMKII) (B), or phospho-GluA1 (P-S831) and total GluA1 (C). Tubulin was used as a loading control. Right: Quantification of Western blots similar to the ones shown on the left. N, number of independent experiments. The p values were determined using the Mann-Whitney test.

Figure 7

doi: https://doi.org/10.1371/journal.pbio.1001262.g007