PINK1 Protects against Oxidative Stress by Phosphorylating Mitochondrial Chaperone TRAP1
Figure 2
PINK1 and TRAP1 Colocalize in the Mitochondrial Inner Membrane and the Intermembrane Space
(A) Post-nuclear supernatants of PC12 cells were fractionated on a 5%–15% linear Optiprep gradient, and the fractions were analyzed by immunoblotting for PINK1, TRAP1, TIMM23, and CANX.
(B) Mitochondria (Mito) isolated from PC12 cells were fractionated into matrix, inner mitochondrial membrane (IM), intermembrane space (IMS), and outer mitochondrial membrane (OM) fractions, and analyzed by immunoblotting for PINK1, TRAP1, and markers of mitochondrial subcompartments: HSPD1 (matrix), TIMM23 (IM), cytochrome c (IMS), and VDAC (OM).