Linking Splicing to Pol II Transcription Stabilizes Pre-mRNAs and Influences Splicing Patterns
(A) Diagrams of the two templates used in the transcription/splicing assay, both contain the first exons of the β-globin gene. The template on the left is headed by the AdML Pol II promoter while the right template is headed by the T7 promoter. (B) Representative autoradiograms of the in vitro transcription/splicing assay appear below the diagrams. Pre-mRNA transcripts were either synthesized by Pol II (left) or T7 polymerase (right). Pre-mRNA transcript, spliced product, and intermediate lariat bands are indicated. (C) Quantitation of the data in (B). Molar concentration of RNA was determined by normalizing counts per min to a known concentration of radiolabeled RNA.