About the Authors

John G Albeck

Contributed equally to this work with: John G Albeck, John M Burke

Affiliation Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America

John M Burke

Contributed equally to this work with: John G Albeck, John M Burke

Affiliations Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America , Biological Engineering Department, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Sabrina L Spencer

Affiliations Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America , Computational and Systems Biology Initiative, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Douglas A Lauffenburger

Affiliations Biological Engineering Department, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America , Computational and Systems Biology Initiative, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Peter K Sorger

To whom correspondence should be addressed. E-mail: peter_sorger@hms.harvard.edu

Affiliations Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America , Biological Engineering Department, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Competing Interests

PKS is a member of the board of directors of Applied Precision Inc., manufacturer of the microscopes used in this study.

Author Contributions

JGA, JMB, SLS, DAL, and PKS worked closely together to design, perform and interpret the experiments; JGA and PKS wrote the paper.