Field validation and application of the luminex triplex HIV assay to estimate HIV prevalence and HIV-1 incidence in Nigeria

Ernest L. Yufenyuy; Olusola A. Akanbi; Vedapuri Shanmugam; Kelsie Decker-Pulice; Jeni Vuong; Mervi Detorio; Amy Zheng; Orji Bassey; Ado G. Abubakar; Oluwaseun Akinmulero; Mudiaga Esiekpe; Andrew Thomas; Iliyasu Abubakar Bichi; Israel Tamunonengiyeofori; Chinwe Ugwu; Evbuomwan Erasogie; William Nwachukwu; Nwando Mba; Ndidi Agala; Megan Bronson; Hetal K. Patel; Nnaemeka C. Iriemenem; Stacie Greby; McPaul I. Okoye; Mahesh Swaminathan; Bharat S. Parekh; Chikwe Ihekweazu

doi:10.1371/journal.pgph.0003455

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Open Access

Peer-reviewed

Research Article

Field validation and application of the luminex triplex HIV assay to estimate HIV prevalence and HIV-1 incidence in Nigeria

Ernest L. Yufenyuy ,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: bsp1@cdc.gov (BSP); yod0@cdc.gov (ELY)

Affiliation Division of Global HIV & TB, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America

https://orcid.org/0000-0002-0613-1373

⨯
Olusola A. Akanbi,

Roles Resources, Supervision, Writing – review & editing

Affiliation Public Health Institute/Centers for Disease Control, Global Health Fellowship Program, Atlanta, Georgia, United States of America
⨯
Vedapuri Shanmugam,

Roles Data curation, Writing – review & editing

Affiliation Division of Global HIV & TB, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America

https://orcid.org/0000-0003-3612-1085

⨯
Kelsie Decker-Pulice,

Roles Data curation, Writing – review & editing

Affiliation Division of Global HIV & TB, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America
⨯
Jeni Vuong,

Roles Data curation, Writing – review & editing

Affiliation Division of Global HIV & TB, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America

https://orcid.org/0000-0002-2038-6356

⨯
Mervi Detorio,

Roles Data curation, Writing – review & editing

Affiliation Division of Global HIV & TB, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America

https://orcid.org/0009-0000-8114-2112

⨯
Amy Zheng,

Roles Formal analysis

Affiliations Division of Global HIV & TB, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America, Public Health Institute/Centers for Disease Control, Global Health Fellowship Program, Atlanta, Georgia, United States of America
⨯
Orji Bassey,

Roles Data curation, Writing – review & editing

Affiliation Division of Global HIV & TB, United States Centers for Disease Control and Prevention, Abuja, Nigeria
⨯
Ado G. Abubakar,

Roles Resources, Supervision, Writing – review & editing

Affiliation Laboratory Quality Improvement Unit, Institute of Human Virology, Abuja, Nigeria
⨯
Oluwaseun Akinmulero,

Roles Data curation, Writing – review & editing

Affiliation Laboratory Quality Improvement Unit, Institute of Human Virology, Abuja, Nigeria
⨯
Mudiaga Esiekpe,

Roles Data curation, Writing – review & editing

Affiliation Laboratory Quality Improvement Unit, Institute of Human Virology, Abuja, Nigeria
⨯
Andrew Thomas,

Roles Data curation, Writing – review & editing

Affiliation Laboratory Quality Improvement Unit, Institute of Human Virology, Abuja, Nigeria
⨯
Iliyasu Abubakar Bichi,

Roles Data curation, Writing – review & editing

Affiliation Laboratory Quality Improvement Unit, Institute of Human Virology, Abuja, Nigeria
⨯
Israel Tamunonengiyeofori,

Roles Data curation, Writing – review & editing

Affiliation Laboratory Quality Improvement Unit, Institute of Human Virology, Abuja, Nigeria
⨯
Chinwe Ugwu,

Roles Data curation, Writing – review & editing

Affiliation Laboratory Quality Improvement Unit, Institute of Human Virology, Abuja, Nigeria
⨯
Evbuomwan Erasogie,

Roles Data curation, Writing – review & editing

Affiliation Nigeria Centre for Disease Control and Prevention, Abuja, Nigeria
⨯
William Nwachukwu,

Roles Resources, Supervision, Writing – review & editing

Affiliation Nigeria Centre for Disease Control and Prevention, Abuja, Nigeria

https://orcid.org/0000-0001-7111-1320

⨯
Nwando Mba,

Roles Resources, Supervision, Writing – review & editing

Affiliation Nigeria Centre for Disease Control and Prevention, Abuja, Nigeria
⨯
Ndidi Agala,

Roles Resources, Supervision, Writing – review & editing

Affiliation Laboratory Quality Improvement Unit, Institute of Human Virology, Abuja, Nigeria
⨯
Megan Bronson,

Roles Resources, Writing – review & editing

Affiliation Division of Global HIV & TB, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America
⨯
Hetal K. Patel,

Roles Resources, Writing – review & editing

Affiliation Division of Global HIV & TB, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America

https://orcid.org/0000-0002-5468-2591

⨯
Nnaemeka C. Iriemenem,

Roles Resources, Supervision, Writing – review & editing

Affiliation Division of Global HIV & TB, United States Centers for Disease Control and Prevention, Abuja, Nigeria

https://orcid.org/0000-0003-2187-8894

⨯
Stacie Greby,

Roles Resources, Supervision, Writing – review & editing

Affiliation Division of Global HIV & TB, United States Centers for Disease Control and Prevention, Abuja, Nigeria
⨯
McPaul I. Okoye,

Roles Resources, Supervision, Writing – review & editing

Affiliation Division of Global HIV & TB, United States Centers for Disease Control and Prevention, Abuja, Nigeria
⨯
Mahesh Swaminathan,

Roles Resources, Supervision, Writing – review & editing

Affiliation Division of Global HIV & TB, United States Centers for Disease Control and Prevention, Abuja, Nigeria

https://orcid.org/0000-0001-5827-3245

⨯
Bharat S. Parekh ,

Roles Conceptualization, Formal analysis, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: bsp1@cdc.gov (BSP); yod0@cdc.gov (ELY)

Affiliation Division of Global HIV & TB, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America

https://orcid.org/0000-0002-1088-4263

⨯
[ ... ],
Chikwe Ihekweazu

Roles Resources, Supervision, Writing – review & editing

Affiliation Nigeria Centre for Disease Control and Prevention, Abuja, Nigeria
⨯
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Field validation and application of the luminex triplex HIV assay to estimate HIV prevalence and HIV-1 incidence in Nigeria

Ernest L. Yufenyuy,
Olusola A. Akanbi,
Vedapuri Shanmugam,
Kelsie Decker-Pulice,
Jeni Vuong,
Mervi Detorio,
Amy Zheng,
Orji Bassey,
Ado G. Abubakar,
Oluwaseun Akinmulero

Published: April 9, 2025
https://doi.org/10.1371/journal.pgph.0003455

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