The respiratory syncytial virus (RSV) RNA-dependent RNA polymerase machinery is localized in cytoplasmic inclusion bodies (IBs), which are regarded as sites of viral RNA synthesis. The RSV transcription factor M2-1 is targeted to these IBs by interacting with the phosphoprotein, the main RNA polymerase cofactor. This interaction is mediated by the “core” domain of M2-1 (See Blondot et al., doi:10.1371/journal.ppat.1002734). The structure of this domain was solved by NMR, and residues involved in the P-M2-1 interaction were revealed. Mutagenesis of these residues on M2-1 (labeled in green) excluded it from IBs where P is still localized (labeled in red).
Image Credit: Marie-Lise Blondot and Jean-François Éléouët, INRA
Opinion
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PLOS Pathogens: published May 3, 2012 | https://doi.org/10.1371/journal.ppat.1002656
Pearls
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