Figures
The novel norovirus virulence factor VF1 localizes to mitochondria.
Murine norovirus expresses a novel protein from an alternative reading frame on the viral subgenomic RNA, which plays a role in the regulation of the innate immune response and apoptosis. VF1 is important for efficient infection of its natural host as viruses lacking VF1 are attenuated in mice (see McFadden et al., doi:10.1371/journal.ppat.1002413). The localization of VF1 to the mitochondria is shown by confocal microscopy. The expression of VF1 N-terminally fused with GFP (green) in transfected Cos7 cells co-localizes with the mitochondrial Mitotracker stain (red), while the nucleus is shown in blue using DAPI staining.
Image Credit: Guia Carrara and Ian Goodfellow, Imperial College London
Citation: (2011) PLoS Pathogens Issue Image | Vol. 7(12) December 2011. PLoS Pathog 7(12): ev07.i12. https://doi.org/10.1371/image.ppat.v07.i12
Published: December 29, 2011
Copyright: © 2011 Carrara, Goodfellow. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Murine norovirus expresses a novel protein from an alternative reading frame on the viral subgenomic RNA, which plays a role in the regulation of the innate immune response and apoptosis. VF1 is important for efficient infection of its natural host as viruses lacking VF1 are attenuated in mice (see McFadden et al., doi:10.1371/journal.ppat.1002413). The localization of VF1 to the mitochondria is shown by confocal microscopy. The expression of VF1 N-terminally fused with GFP (green) in transfected Cos7 cells co-localizes with the mitochondrial Mitotracker stain (red), while the nucleus is shown in blue using DAPI staining.
Image Credit: Guia Carrara and Ian Goodfellow, Imperial College London