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Correction: A Polymorphism in the Processing Body Component Ge-1 Controls Resistance to a Naturally Occurring Rhabdovirus in Drosophila

  • Chuan Cao,
  • Michael M. Magwire,
  • Florian Bayer,
  • Francis M. Jiggins

Correction: A Polymorphism in the Processing Body Component Ge-1 Controls Resistance to a Naturally Occurring Rhabdovirus in Drosophila

  • Chuan Cao, 
  • Michael M. Magwire, 
  • Florian Bayer, 
  • Francis M. Jiggins
PLOS
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The authors would like to correct four errors in the manuscript:

  1. In Fig 5C, the relative viral titre bars are at the wrong height. In the figure, the Ge-1(S) ctrl (grey bar) and Ge-1(H) ctrl (grey bar) have higher relative viral titre than Ge-1(S) DCP1 KD (red bar) and Ge-1(H) DCP1 KD (blue bar). However, the DCP1 KD should have higher viral titres than the controls in both S and H backgrounds. This mistake was made in R script when the figure was plotted. The text and legend are all correct and unaffected. Please see the corrected Fig 5 here.
  2. The X labels of S2 Fig overlap. Label "Edc3" overlaps with each Ctrl1. This mistake is due to formatting errors. Unlike the error in Fig 5C this is just a cosmetic change. Please see the corrected S2 Fig here.
  3. In the Results section, subsection "The resistant allele of Ge-1 contains a 26 amino acid deletion", the authors would like to change the last sentence of the first paragraph of this section (Line 170–171) to: “We found that there was no consistent difference in gene expression in the resistant and susceptible lines at six days and twelve days after they had been injected with the virus (Fig 2D; day6: F1,76 = 0.0189,172 p = 0.891; day12: F1,97 = 6.109, p = 0.015)." This sentence describes the result more accurately.
  4. There is an error in the Results section, subsection "Deleting the 26 amino acid region of Ge-1 in transgenic flies makes them resistant to DMelSV". The word "higher" should be replaced with "lower" in the last sentence of the first paragraph of this section (Line 217). The corrected sentence is: "Similarly, viral titres were 512-fold higher in the transgenic line with the deletion than the three lines without the deletion (Fig 4B; F1,56 = 156.9, P<<0.001)." The results and conclusions are unaffected.
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Fig 5. The effect of Ago2 and DCP1 on susceptibility to DMelSV.

(A) and (B) are the effect of Ge-1 on susceptibility in Ago251B mutant flies. Blue represents flies with the resistant Ge-1 allele and Ago2 mutant, black the resistant Ge-1 allele and Ago2 wild-type, red the Ge-1 susceptible allele and Ago2 mutant, and orange the susceptible Ge-1 allele and Ago2 wild-type. (A) CO2 sensitivity assay. (B) Relative viral titre estimated by quantitative PCR relative to RpL32 was used as reference gene. (C) The effect of knocking down DCP1 on viral titre in Ge-1 susceptible backgrounds (red bar) and Ge-1 heterozygous backgrounds (blue bar). Grey bars are controls for the RNAi. Error bars are standard errors.

https://doi.org/10.1371/journal.ppat.1005730.g001

Supporting Information

S2 Fig. RNAi knock-downs of P-body components.

Eight genes encoding P-body components were knocked down by RNAi: Edc3 (CG6311), DCP2 (CG6169), DCP1 (CG11183), GW182 (CG31992), pcm (CG3291), me31B (CG4916), Part-1 (CG5208) and stau (CG5753)). Left 10 boxes (red) are RNAi knock-downs in flies with Ge-1 susceptible background. Right 10 boxes (blue) are RNAi knock-downs of flies that were heterozygous for the resistant and susceptible Ge-1 alleles. Each dot represents one sample which is a pool of 10–15 flies. There were two different genetic backgrounds: round dots represent KK library RNAi lines and triangles GD library RNAi lines. The flies were reared at 18C where expression of the RNAi construct is inefficient and then transferred as adults to 25C. There was a significant heterogeneity among the KK library RNAi lines (F = 7.29, P = 3.6x10-6) but not among the GD lines.

https://doi.org/10.1371/journal.ppat.1005730.s001

(TIF)

Reference

  1. 1. Cao C, Magwire MM, Bayer F, Jiggins FM (2016) A Polymorphism in the Processing Body Component Ge-1 Controls Resistance to a Naturally Occurring Rhabdovirus in Drosophila. PLoS Pathog 12(1): e1005387. pmid:26799957