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Hybridization in Parasites: Consequences for Adaptive Evolution, Pathogenesis, and Public Health in a Changing World

  • Kayla C. King ,

    Contributed equally to this work with: Kayla C. King, Rike B. Stelkens, Joanne P. Webster

    Affiliation Department of Zoology, University of Oxford, Oxford, United Kingdom

  • Rike B. Stelkens ,

    Contributed equally to this work with: Kayla C. King, Rike B. Stelkens, Joanne P. Webster

    Affiliation Max Planck Institute for Evolutionary Biology, Plön, Germany

  • Joanne P. Webster ,

    Contributed equally to this work with: Kayla C. King, Rike B. Stelkens, Joanne P. Webster

    Affiliation Department of Pathology and Pathogen Biology, Centre for Emerging, Endemic and Exotic Diseases (CEEED), Royal Veterinary College, University of London, London, United Kingdom

  • Deborah F. Smith,

    Affiliation Department of Biology, University of York, York, United Kingdom

  • Michael A. Brockhurst

    michael.brockhurst@york.ac.uk

    Affiliation Department of Biology, University of York, York, United Kingdom

Hybridization in Parasites: Consequences for Adaptive Evolution, Pathogenesis, and Public Health in a Changing World

  • Kayla C. King, 
  • Rike B. Stelkens, 
  • Joanne P. Webster, 
  • Deborah F. Smith, 
  • Michael A. Brockhurst
PLOS
x

Hybridization of parasites is an emerging public health concern at the interface of infectious disease biology and evolution. Increasing economic development, human migration, global trade, and climate change are all shifting the geographic distribution of existing human, livestock, companion animal, and wildlife parasites [19]. As a result, human populations encounter new infections more frequently, and coinfection by multiple parasites from different lineages or species within individual hosts occurs. Coinfection may have a large impact on the hosts and parasites involved, often as a result of synergistic or antagonistic interactions between parasites [10]. Indeed, mixed-species coinfections have been found to influence parasite establishment, growth, maturation, reproductive success, and/or drug efficacy [1113]. However, coinfections can allow for heterospecific (between-species or between-lineage) mate pairings, resulting in parthenogenesis (asexual reproduction in which eggs occur without fertilization), introgression (the introduction of single genes or chromosomal regions from one species into that of another through repeated backcrossing), and whole-genome admixture through hybridization [14].

Recent molecular developments have revealed instances of fertile hybridization and introgression in plants [15], animals [16,17], and humans [18]. However, modern genetics and genomics have also uncovered the first confirmed cases of introgression within eukaryotic parasites [14,19]. Examples for such successful reticulate evolution in parasites include, but are not exclusive to, causative agents of important diseases initiated by fungi (Cryptococcus [20]), helminthic worms (Schistosoma, Fasciola, Ascaris, and Trichinella [21,2224]), and protozoa (Plasmodium, Leishmania, Toxoplasma, and Trypanosoma [25,2631]), as well as their vectors. These cases involve introgression between members of the neglected tropical diseases (NTDs) and/or neglected zoonotic diseases (NZDs)—highly debilitating diseases infecting more than a sixth of the world’s human population (and their livestock), with devastating consequences for individuals and communities. Such diseases are predominantly diseases of the world’s poorest communities, afflicting those people who are most at risk of contracting mixed parasitic infections (and hence also at risk for potential novel introgressed parasite infections) and at the same time least likely to get proper medical care once infected [32].

Evidence gathered to date, mainly from studies on nonparasitic animals and plants, suggests that hybridization can have a major evolutionary impact [33]. While hybridization can result in lower average fitness due to F1 sterility and inviability, caused by negative epistatic interactions [3436] and the disruption of beneficial gene complexes [37,38], hybridization is at the same time a rich source of new genetic variation [39]. This can provide the raw material for natural selection to shape the evolution of ecologically relevant traits [33,40,41]. Thus, while a hybrid offspring, under certain conditions, may be less fit than its purebred offspring counterparts from either parental line, hybridization broadens the “working surface” for selection by producing a whole range of potentially adaptive phenotypes, ranging from one parent to the other and beyond. In nonparasitic taxa, hybridization has already been shown to promote geographic range expansions of populations (for example, in spiders [42]) as well as adaptation to new environments (Darwin finches [43]). There is also increasing evidence that hybridization can lead to the functional diversification of a group, as shown in Helianthus sunflowers [44], and even to speciation (reviewed in [40,45,46]). One key example of this is the case of “transgressive hybrids,” i.e., hybrids expressing extreme phenotypes that do not reside in either of the parental populations. These are “hopeful monsters” with respect to their evolutionary potential because they can diverge from their parents ecologically and may quickly become reproductively isolated from them [4749]. In addition to broadening the selection surface, hybridization can also speed up the process of adaptation. After hybridization events, new alleles or allelic combinations that happen to be beneficial in the new environment are available immediately. This is in contrast to nonhybrid populations in which adaptation relies on alleles brought in via immigration or de novo mutation, both processes that require relatively longer periods of time [50,51]. As a consequence, populations with a hybrid origin may survive rapid environmental change better than their nonhybrid parents, as recently shown in yeast [52].

We predict that parasites are one of the major groups of organisms in which hybridization can have major impacts on the evolution and diversification of a group [14,19] and can lead to speciation [53], affecting key pathogenic traits and transmission. Hybridization in parasites may thus pose a serious challenge for the prevention, effective control, and therapy of disease [19]. While it has been suggested that hybridization and introgression between parasites can potentially drive the emergence and rapid evolution of novel zoonotic diseases [14], most studies to date are descriptive and do not consider the evolutionary consequences. Here, we review some of the most recent advances in the detection of hybridization in eukaryotic parasites (and their vectors) and discuss the significance of parasite hybridization for adaptive evolution and public health supported by relevant case studies in Schistosoma spp. (Box 1), Leishmania and Trypanosoma (Box 2), and the malaria vector Anopheles (Box 3). Given the role of infectious agents in our changing world, particularly in terms of emerging parasitic disease in response to anthropogenic change [32], it is time for a new and integrative perspective. Here, we argue for the integration of parasitology, disease biology, and evolutionary biology to understand the consequences of parasite hybridization to aid in the management and prevention of disease.

Box 1. Case Example—Introgression in Helminths: Schistosomiasis

Schistosoma spp. are the causative agents of schistosomiasis, a prevalent, chronic, and debilitating helminthic disease of humans and animals that occurs across much of the developing world. As early as 1948, there have been phenotypic reports of eggs indicative of potential Schistosoma haematobium–S. mattheei hybrids in Rhodesia/Zimbabwe [75], S. bovis–S. curassoni hybrids in West African ruminants, S. haematobium–S. mattheei hybrids in Southern African ruminants, and S. haematobium–S. bovis hybrids in humans from West Africa [7678]. More recently, ITS1+2 and cox1 barcoding studies of viable schistosome miracidial larvae hatched from the stool and urine of Senegalese school children confirmed bidirectional hybridization between human S. haematocium and livestock S. bovis [67], as well as for S. haematobium and S. curassoni [79]. Studies from infected snails in Kenya have observed hybrid cercariae between S. mansoni from humans and its sister species, S. rodhaini, from rodents [80]. These authors, using microsatellite markers (ribosomal DNA [rDNA] and mitochondrial DNA [mtDNA]), demonstrated that these hybrids produce viable offspring through first or successive generation backcrosses with S. mansoni. Unlike the S. haematobium and S. bovis or S. currassoni hybrids described above, the direction of introgression appeared highly asymmetric, causing unidirectional gene flow from the rodent S. rodhaini to the human S. mansoni [80]. Recent evidence from infected humans in Senegal has also revealed the potential for introgressions between the more phylogenetically distant pairings of the two major human schistosome species in Africa, S. haematobium and S. mansoni, a pairing previously thought to result in unviable eggs exclusively through parthogenesis [81]. These studies combined provide convincing evidence that schistosome species readily hybridize in nature, which may have major implications in light of the current global push for human disease control programmes to shift from controlling morbidity to halting transmission [32]. How such introgression may alter host range is perhaps the most pressing area for future research. Many schistosome species infecting livestock have a broader geographical range beyond Asia and Africa, with compatible snail intermediate hosts present. Novel zoonotic hybrids may therefore have the potential to be a global disease, particularly in our current climate of global warming and increased human and animal movement and transportation. This may be highlighted most clearly where novel introgressed hybrids between human S. haematobium and livestock S. bovis have recently been identified, with substantial ongoing transmission amongst both local residents and tourists, within Europe [8284].

Box 2. Case Example—Introgression in Protozoans: Leishmaniosis and Trypanosomiasis

Introgressions can occur within the causative agents of protozoal diseases leishmaniasis and trypanosomiasis (Kinetoplastida: Trypanosomatidae) [8588]. Approximately 30,000 people in 36 countries of sub-Saharan Africa suffer from human African trypanosomiasis (HAT), and Chagas disease has been classed as the most important vector-borne infection in Latin America, affecting an estimated 7–8 million humans, with around 21,000 deaths per year [89]. Leishmania parasites are another of the most important vector-borne pathogens in the developing world. Both Leishmania and Trypanosoma are also parasites with major zoonotic reservoirs. Two of the major lineages of Trypanosoma cruzi (discrete typing units [DTUs] III and IV) are now thought to have arisen by intraspecific hybridisation [87], despite their predominant mode of asexual reproduction, and introgression between subspecies has been associated with virulence [88]. Similarly, whilst asexual reproduction through clonal propagation has been proposed to be the major reproductive mechanism across the genus Leishmania, a hybridizing sexual cycle has been detected within its sand fly vector from across a range of geographical locations [90,91]. Whole-genome sequencing of Leishmania parasites isolated from sand flies from a Turkish endemic area indicated that variation in these parasites arose following a single cross between two phylogenetically distinct strains. Furthermore, whilst it appears that these populations do reproduce primarily clonally following this original hybridization event, subsequent recombination between the progeny does also occur [92]. The potentially large epidemiological consequences of such recombination events may be demonstrated by the observation that Leishmania infantum/L. major hybrids possess an enhanced host range, as hybrid offspring, and, unlike their parental single species, they are able to infect another vector, Phlebotomus papatasi [93].

Box 3. Case Example—Introgression in the Parasite Vector Anopheles: Malaria

Anopheles has become a model organism at the interface of speciation genomics and epidemiology [94], showcasing the potential perils of hybridization for public health but also the possible benefits of using controlled and induced hybridization as a means for disease control. Anopheles mosquitoes are vectors for malaria, which is caused by parasitic protozoans belonging to the genus Plasmodium. Malaria affects 200 million people a year worldwide, with an estimated 0.5–1 million deaths per year, mostly among young children in sub-Saharan Africa, where 90% of the world’s malaria deaths occur [95].

There are approximately 60 different species of Anopheles found worldwide [96], and genomic sequences of 16 Anopheles species (including vector and nonvector species) have recently become available [94,97]. These sequences have revealed fast and flexible evolutionary rates with respect to traits affecting their transmission potential, shown extensive introgression between Anopheles species, and suggested that enhanced vectorial capacity and adaptation to humans as primary hosts can result from interspecific genetic exchange. A multilocus single nucleotide polymorphism (SNP) genotyping panel is also at hand to detect F1 hybrids and backcrosses between the main vectors of malaria, Anopheles gambiae sensu stricto (S form) and A. coluzzii (M form) [98].

Control of Anopheles through insecticides, such as dichlorodiphenyltrichloroethane (DDT) and pyrethroids, has contributed to the prevention of malaria, but resistance to insecticides has recently emerged in Anopheles populations [99,100]. Recent studies have demonstrated rapid adaptive introgression of the insecticide resistance mutation Vgsc-L1014F from A. gambiae to A. coluzzii, in response to strong anthropogenic selection from increased insecticide use [101,102]. Others have found gene flow occurring at rates “far from inconsequential” between other species of Anopheles (e.g., between A. gambiae and A. arabiensis in Uganda [99,103] and A. sinensis and A. kleini in Korea [104]). Experimental interpopulation crosses of A. gambiae, monitored for traits determining their malaria transmission potential, scored higher for fecundity, body size, adult longevity, and average blood meal size, compared to both parental strains [105]. It is thus conceivable that the fitness-enhancing potential of hybridization may also apply to closely related interspecific hybrid crosses.

Hybridization between Anopheles species may have beneficial effects for disease control. Asymmetric introgression has recently been shown to transfer adaptive immunity and increased refractoriness to the Plasmodium pathogen from A. coluzzii to A. gambiae in Guinea [106]. Another opportunity for disease control is to modify the host preference of A. gambiae from humans to cattle by hybridizing it with A. quadriannulatus, a more zoophilic nonvector species, as a strategy to decrease its competence as malaria vector [107].

Consequences of Hybridization in Parasites

Since multidrug resistance became a worldwide problem in pathogenic bacteria in the 1950s [54], we know that the exchange of genetic material via horizontal gene transfer among bacterial taxa has contributed to their evolution and pathogenesis [55,56]. Horizontal gene transfer can be advantageous and confer higher fitness, for instance, through the acquisition of antibiotic drug resistance [57] or through the spread of virulence factors; a well-studied example is the Shiga toxin genes exchanged between Escherichia coli and Shigella bacterial pathogens [58]. There are also several examples of recombinant human viruses that have exchanged genes with other strains with detrimental effects (Spanish flu, human rotavirus, and dengue fever; [59,60,61]). Conversely, despite their equally negative impact on host populations, not much is known about the hybridization of eukaryotic parasites, their frequency in the wild, or how hybridization may affect their spread and pathogenicity. Modern molecular techniques, however, can expose the signature of hybridization in the genome more rapidly and accurately, thereby increasing the number of recent reports of parasite hybridization.

We outline what we believe will be the most important and/or potentially dangerous effects of hybridization in eukaryotic parasites: (1) the generation of novel and extreme infection phenotypes, (2) an increase in host range, being just one component of (3) an increase in transmission potential, (4) an increase in parasite evolutionary potential with consequences for host–parasite coevolution, (5) the breakdown of host-specific adaptations, and also (6) an altered response to drug therapy (Fig 1).

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Fig 1. Schematic of the drivers and consequences of parasite hybridization.

http://dx.doi.org/10.1371/journal.ppat.1005098.g001

  1. As a result of new allelic combinations produced by hybridization, hybrid parasites may show enhanced phenotypic characteristics compared to the parents. Hybrids may be better at host exploitation, such that they may be more efficient at obtaining nutrition from the host, leading to higher fecundity or faster maturation time. Also, certain new hybrid parasite genotypes have been shown to be better at avoiding recognition and resistance from the host’s adaptive immune system, potentially leading to higher infectivity and unusual pathologies [29,62]. We thus predict that hybrid parasites are able to exploit novel resources and sites of infection within the host, which parental genotypes cannot utilize.
  2. Hybrid parasites may be able to infect a greater range of host species [14,19,63]. While parental parasites are often restricted to one host species, hybrid parasites may be able to exploit both. Interspecific Schistosoma spp. hybrids, for instance, are able to infect both parental intermediate snail hosts (Box 1) [21]. In addition, just as some hybrids of nonparasitic taxa adapt to new ecological conditions and colonize novel environments that neither of the parents can survive [44,49], we would predict that hybrid parasites may be able to infect entirely new host species. As an indication that this indeed occurs in the wild, two plant pathogenic fungi (Phytophthora spp. infecting alder trees [64] and Zymoseptoria pseudotritici infecting a range of grasses [53]) and one amphibian fungal pathogen (a new lineage of Batrachochytrium dendrobatidis causing dramatic outbreaks of chytridiomycosis [65]), which all emerged via hybridization, have been found to live on host species neither of their parents are found on.
  3. With increasing land use and current rates of anthropogenic change across both the environment and agricultural/livestock practices, we predict that recombinant parasite genotypes will be generated at an increasing rate. Encounters between previously isolated parasite species become more frequent when geographic and ecological barriers that previously existed between parasite species are lost. Taking together the potential impact of hybrid superiority and host range expansions (described in 2) and an increase in anthropogenically mediated parasite dispersal, the transmission of disease may become considerably facilitated [66,67]. This may lead to epidemics, threaten global food security, and endanger natural animal and plant populations. For instance, the increasing creation and alteration of water bodies for agriculture can lead to areas of overlap and co-occurrence of the different intermediate snail host species of Schistosoma parasites [66,68]. This generates ample opportunity for coinfections of the definitive host by different Schistosoma species, which may then hybridize. In addition, humans and livestock are drawn to the same water resources, where they can both release parasites and become infected, thus creating a “hotbed” for disease transmission between human and livestock.
  4. Evolutionary theory predicts that elevated virulence and genetic diversity, both potential outcomes of parasite hybridization, can increase the evolutionary potential of parasites and alter the host–parasite coevolutionary process [69]. For instance, for Phytophthora, hybridization has led to a range of new species with particularly aggressive pathogenicity [64], and hybrid lineages of the amphibian zoosporic fungus Batrachochytrium, which cause dramatic outbreaks of chytridiomycosis, have been found to be hypervirulent to many hosts [65]. In the context of coevolutionary races, higher virulence of hybrid parasites may impose stronger selection for host resistance.
  5. Hybridization in parasites could, however, also be maladaptive for the parasites because of fitness-reducing genetic incompatibilities (negative epistasis) and the breakdown of host-specific adaptations, potentially leading to reduced infectivity/virulence. While such outbreeding depression is well known from free-living organisms, studies on the fitness of hybrid parasites are scarce. Outbreeding depression has been reported in a snail–trematode host–parasite system (Potamopyrgus antipodarum–Microphallus sp.), in which hybrid parasites suffer from reduced infectivity in both parental host populations [70,71]. In such cases, we predict that hybrid parasites may be less likely to evade host immunity or they may fail to effectively colonize the correct organ or body site. Through recombination and segregation, hybrids may also lose beneficial alleles that the parents had acquired previously, such as those conferring drug resistance (see below). Hybrid parasite inferiority can also lead to unusual pathogensis, which may hamper diagnosis, particularly when two very different disease phenotype parasites are introgressed (Box 1). As such, hybridization between divergent parasite populations may both promote and alter pathogenesis, which has important implications on disease prevalence, pathology, and treatment.
  6. Hybridization may have a wide range of effects on drug efficacy in parasites. On the one hand, zoonotic hybrids may exhibit enhanced susceptibility to drugs if resistance alleles circulating in the human host parasite population are swamped by introgression of drug-susceptible alleles from the animal host parasite population. Furthermore, if hybridization between human and animal parasites increases the host range from human host specificity to that of a large animal host range reservoir, this could act against the establishment and spread of drug resistance [32]. Alternatively, hybridization could potentiate adaptive evolution within certain parasites in response to drug treatment (Box 1). One example for this is Cryptococcus serotype hybrids that are resistant to an antifungal drug usually used to treat infections with the individual parental serotypes [20]. More evidence for the adaptive introgression of drug resistance genes from nonsusceptible strains or species comes from Haemonchus nematodes that gained resistance against the broad-spectrum drug ivermectin through hybridization [72,73] and the introgression of anticoagulant rodent poison resistance by hybridization between Old World mice [74].

Conclusions

Understanding and monitoring hybridization in animal and human parasites will be essential for optimizing and evaluating control strategies across potential hybrid zones. Relatively simple diagnostic procedures currently exist for detecting hybrids in some parasite groups (e.g., using a multilocus approach with mitochondrial cytochrome c oxidase subunit 1 (COX-1)/internal transcribed spacer (ITS) barcoding; [67]). However, the advancement of state-of-the-art genomic technologies will be helpful for more fine-scale detection of hybridization in parasites, to determine their evolutionary rates, and to identify patterns of cross transmission between host species (i.e., sources of transmission of zoonotic parasites to humans). Since hybrid parasites appear to be a particular problem in NTDs, there is a need to develop cheap, robust diagnostics appropriate to use in the field.

The circumstances producing increased opportunity for hybridization are the same ones that cause increased rates for disease distribution and transmission. Thus, the likelihood of parasite hybridization is increasing with the intensification of world trade of plants and animals, human migration, land use, and drug administration (Fig 1). Interspecific hybridization and introgression appear to be viable strategies for many parasites to maintain transmission, with potentially major impacts on the evolution of virulence, infection persistence, drug resistance, and host range, as well as on the clinical outcomes of disease. The combined effects of anthropogenic distribution and increased hybridization opportunities could have hazardous and catalytic effects on epidemiology, imposing further challenges and constraints for their effective control. More empirical work on the differential transmission, infectivity, drug efficacy, pathogenesis, and evolution of hybrid parasite populations is therefore urgently required to guide policies on their monitoring and management.

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