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How Does the Dinoflagellate Parasite Hematodinium Outsmart the Immune System of Its Crustacean Hosts?

How Does the Dinoflagellate Parasite Hematodinium Outsmart the Immune System of Its Crustacean Hosts?

  • Andrew F. Rowley, 
  • Amanda L. Smith, 
  • Charlotte E. Davies
PLOS
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Hematodinium Infections: A Problem for the Sustainability of Crustacean Fisheries and Aquaculture Worldwide

Dinoflagellates of the genus Hematodinium infect over 40 species of marine crustaceans. Since the initial description of this parasite in France in the 1930s [1], it appears to have spread globally, causing economic loss in some species, including Tanner crabs (Chionoecetes bairdi) in Alaska [2], blue crabs (Callinectes sapidus) in the United States [3], edible crabs (Cancer pagurus) and Norway lobsters (Nephrops norvegicus) in Europe [47], sand crabs (Portunus armatus) in Australia [8], and Chinese swimming crabs (Portunus trituberculatus) in northern China [9]. Because this disease is thought to be fatal, it may be of great significance to the sustainability of both captive shellfish fisheries and aquaculture [10,11]. For instance, the decline in blue crabs from the Atlantic to Gulf Coasts in the US appears to be linked to epizootic outbreaks of disease caused by Hematodinium [12]. Similarly, the reduction in velvet swimmer crab (Necora puber) numbers in Brittany in the 1980s has been attributed to the high prevalence of such infections [13]. Finally, the recent reports of Hematodinium infections in crabs and shrimp raised under aquaculture conditions in China [9,14,15] highlight the danger to additional, cultured crustacean populations.

The number and host range of species of Hematodinium is unclear. Chatton and Poisson [1] identified one species of this parasite that they termed H. perezi. Notably, this species of parasite was reported in two different species of crabs from several locations around the French coast. A second species, H. australis, was first described in sand crabs from Moreton Bay in Australia [8]. Recent studies making use of the variability of the ITS1 rDNA region of H. perezi suggest that there are three clades (genotypes), I–III, of this species (see [11] for further details). Pagenkopp Lohan et al. [16] concluded that blue crab populations collected from the Atlantic to the Gulf coasts of the US were all affected by one genotype, H. perezi (III), which implies a large geographic range. Importantly, other species of crabs collected from the same region were also infected by the same genotype of H. perezi [16]. In Europe, there may be two clades of Hematodinium infecting a range of crabs. H. perezi genotype I has been reported in Liocarcinus depurator [17] and Carcinus maenas. The second species of Hematodinium, currently unnamed (Hematodinium sp.) infects a wider range of crustaceans, including edible crabs (C. pagurus). Importantly, these observations imply that this parasite is a host generalist and may be able to “jump” from host species to species, thereby making it a significant threat to a wide variety of commercially important decapods.

What Is the Interaction between the Host and Hematodinium?

Reports on the pathology of Hematodinium spp. infections in a variety of crustacean hosts are all similar. While the mode of transmission of the parasite to its hosts is unclear, it is thought that susceptible animals may become infected after moulting [18,19], suggesting an integumentary route of invasion when the cuticle is soft and vulnerable. The parasites gain entry to the underlying tissues, including the main body space, termed the haemocoel, and multiply in tissues and organ systems including the heart, hepatopancreas, connective tissue, and the haemolymph (“blood”) [10]. The timescale of infection is protracted in some host species of crustacean, such as those living in colder waters, where the period from infection to death may be several months [2], whereas in others, it can be much more rapid [20]. Early phases of infection (see “Early Phase” in Fig 1) are characterised by limited changes in the number of circulating haemocytes and only modest elevation in parasite abundance in this tissue. One of the hallmarks of advanced Hematodinium infections (“Late Phase” in Fig 1) is the marked multiplication of the parasites in all tissues and a simultaneous reduction in the number of circulating haemocytes, leaving the affected animals unable to mount effective cellular defence and haemostatic responses [20]. Accompanying the increase in the number of parasites in advanced infections comes muscle necrosis [21] and “metabolic exhaustion” [22], characterised by significant reduction in plasma proteins, including haemocyanin that serves as both a respiratory pigment and as an immunologically active molecule [23]. Interestingly, these pathological changes may be reflected in alteration in the general behaviour of infected animals [24,25], leaving them more susceptible to predation and loss from the fishery.

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Fig 1. Interaction of Hematodinium with its crustacean host.

At an early phase of infection there is a small number of parasites in the haemolymph (unlabelled arrows) and a large number of haemocytes in circulation, as found in uninfected individuals (spread cells in micrograph). In late-phase infections, there are often large masses of Hematodinium in the haemolymph and very few circulating haemocytes. This reduction in haemocytes results in an increased likelihood of secondary infections and prolonged bleeding times following wounding. Death probably ensues as a result of parasite development and utilisation of host’s metabolic products, as well as a heightened chance of secondary infections.

https://doi.org/10.1371/journal.ppat.1004724.g001

Is There Evidence of Immune Reactivity to the Presence of Hematodinium in Crustacean Hosts?

The immune system of invertebrates has been extensively studied, especially in insects and crustaceans. However, our understanding of the interaction between pathogens and parasites and their hosts is less well understood, with the notable exception of those agents that infect humans while in their invertebrate vectors. For a brief description of the main features of the crustacean immune system, the reader is directed to Box 1 in this article and recent reviews [26,27].

Box 1. The Invertebrate Immune System: A Brief Primer

The invertebrate immune system consists of cellular and humoral defences that are principally mediated by blood cells, which in arthropods are termed haemocytes. Non-self is recognised by a variety of immune factors, including lectins and products of the prophenoloxidase cascade [26,27]. The main cellular defence reactions are phagocytosis and a process in which pathogens and parasites are walled off, called nodule formation or encapsulation. The blood (haemolymph) contains a wide variety of antimicrobial factors including hydrolytic enzymes (e.g., lysozyme) and antimicrobial peptides (e.g., defensins). Although the defence reactions of invertebrates are considered to be non-specific, recent studies have questioned whether a unique form of immune memory is present at least in some invertebrates [27].

A significant number of studies have examined the pathology of Hematodinium infections in a wide range of crustacean hosts (see [10] for review). Although in some cases there has been evidence provided of a cellular host response to this parasite, such as the presence of haemocytic nodules [6], close examination reveals there is little or no evidence for the presence of the various stages of this parasite within the nodules, implying that their generation is not a direct response to the parasite. A potential explanation for the presence of nodules is as a result of other co-infections—which are common in some crustaceans [2831]—or tissue damage caused by Hematodinium in late-phase infections (Fig 1) [31]. One report has produced convincing evidence for the uptake of Hematodinium by the fixed phagocytes of N. norvegicus [32], but to our knowledge no other studies have found such an association.

To further investigate this apparent lack of host response to the presence of Hematodinium, we injected juvenile edible crabs (C. pagurus) with Hematodinium and monitored changes in the haemogramme. These experiments found no evidence for reduction in the number of circulating haemocytes that might have resulted from localised nodule formation, and histological examination of various tissues, including the gills, failed to show the presence of nodules indicative of such a host response. Furthermore, seven days post-challenge, 100% of experimental animals had stages of the Hematodinium parasite free in the haemolymph (Fig 2A) implying that they had not been cleared from circulation by either fixed or free phagocytes. These studies support the concept of a failure of the immune defences to recognise and clear these parasites from the haemocoel. Hence, it can be concluded that Hematodinium spp., regardless of the host species studied, are not effectively recognised as foreign by the immune system.

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Fig 2. Experimental exploration of the interaction between Hematodinium and its host.

(A). Histological appearance of gill lamellae of a crab seven days post-injection of Hematodinium. Note these parasites in the gill space (unlabelled arrow). Scale bar = 10 μm. (B). Clearance of the gram-positive bacterium, Bacillus subtilis from Hematodinium-infected (early-stage infection) and uninfected juvenile edible crabs (C. pagurus). Crabs were injected intrahaemocoelically with 105 live bacteria and the numbers of remaining viable bacteria (cfu) in the circulation determined by standard plate counting on tryptone soya agar + 2% NaCl plates. Values shown are means of cfu with standard error of the mean (SEM), n = 5. The values are not statistically different between animals of differing disease status.

https://doi.org/10.1371/journal.ppat.1004724.g002

What Are the Potential Mechanisms Employed by Hematodinium to Avoid Immune Recognition and Elimination?

There are several recognised strategies employed by parasites and pathogens to successfully colonise their hosts without their elimination by the cellular and humoral components of the immune system (see Box 1). Table 1 summarises and speculates on some of the more likely mechanisms that Hematodinium could use, and some of these are now explored.

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Table 1. An exploration of potential mechanisms employed by Hematodinium to avoid, circumvent, or suppress the crustacean immune system.

https://doi.org/10.1371/journal.ppat.1004724.t001

As haemocytes are the cells at the centre of the invertebrate immune system and are also responsible for blood coagulation, any reduction in their numbers has a profound effect on the survival of the infected host. One of the hallmarks of late-phase Hematodinium infections is the reduction in the number of circulating haemocytes that leaves the host unable to mount an effective cellular defence and haemostatic response [20]. The paucity of haemocytes in circulation could reflect their loss due to extensive infiltration of infected tissues but there is no evidence for this event from histological studies (e.g. [7]) and it is more likely that it results from suppression of haemopoietic activity. As we have shown that haemocytopaenia is a relatively late response to advanced infections, at least in the edible crab (Fig 1), this implies that the principal mechanism of pathogenicity does not involve suppression of haemopoiesis. Furthermore, we would argue that this loss of haemocytes is probably linked to the state of metabolic exhaustion [20,22] caused by the rapidly multiplying parasites “stealing” host resources, rather than any parasite-driven direct inhibition of haemopoiesis.

Some crustaceans with Hematodinium have been found to be susceptible to secondary infections caused by other parasites and pathogens [10,28,29]. One example of this interaction comes from studies with naturally Hematodinium-infected crabs that were found to have a secondary fungal infection [28]. These authors concluded that the presence of Hematodinium caused the host to be susceptible to these infections because they only found the fungus in Hematodinium-affected crabs. They also discussed the possibility that host immunosuppression caused by the presence of Hematodinium left these animals susceptible to this and other secondary infections. To investigate this putative interaction further, Smith et al. [33] challenged Hematodinium-infected and uninfected crabs with the fungus (now identified as a member of the Ophiocordyceps clade). Unexpectedly, the presence of Hematodinium apparently caused a reduction in the multiplication of the fungus in the haemocoel in comparison to Hematodinium-free crabs. However, all crabs, regardless of their infection status, died as a result of the fungal infection. Furthermore, the fungal infection was found in small numbers of edible crabs in the wild that were uninfected by Hematodinium, reflecting that the association between the two pathogens was not obligate [33]. These co-infections also reveal that crustaceans infected by Hematodinium are able to mount an efficient immune response to other pathogens that is not apparently different to that seen in uninfected hosts. Hence, these observations advocate that Hematodinium does not elicit general immune suppression. However, a recent study in which Chinese swimming crabs (Portunus trituberculatus) were challenged with Hematodinium, has provided evidence of an apparent immunosuppressive action by these parasites [34]. The study revealed that artificially infected crabs showed temporal changes in prophenoloxidase gene expression post-challenge. The prophenoloxidase activating system is a key component of the crustacean immune response (Box 1) [26], and therefore, changes in this are likely to adversely affect host survival. Although the authors concluded that Hematodinium showed the ability to cause potential inhibition of the prophenoloxidase activating system, levels of enzyme (i.e., phenoloxidase) activity varied post-challenge from no significant changes through to both elevation and suppression at the different time points studied.

To further investigate the possibility that Hematodinium causes generalised immunosuppression, we challenged juvenile edible crabs, with or without low-severity (i.e., early-phase) Hematodinium infections, with the non-pathogenic bacterium Bacillus subtilis, and its clearance from circulation was monitored by standard plate counts. These experiments demonstrated that crabs with low-severity Hematodinium infections showed no changes in their ability to clear the bacteria from circulation in comparison to uninfected crabs (Fig 2B). This implies that crabs with early-phase Hematodinium infections are not immunocompromised towards other potential bacterial pathogens. Overall, it is our opinion that generalised immune suppression is unlikely to be the main strategy utilised by Hematodinium to overwhelm its host.

The apparent lack of any cell–mediated response directed to the presence of large numbers of Hematodinium in the haemolymph points to the possibility that this parasite utilises molecular mimicry or evasion to “hide” from the host’s immune system. While there may be other strategies employed, this would seem the most likely explanation (Table 1). Several host–parasite relationships in which molecular mimicry or specific evasion operates have been investigated using invertebrate models and these provide us with mechanistic explanations that may shed light on the current crustacean–Hematodinium interaction. Two good examples of such models include the human malaria parasite, Plasmodium, in its invertebrate vectors, the anopheline mosquitoes [35,36], and Schistosoma mansoni in the freshwater snail, Biomphalaria glabrata [37,38].

Lectins are key immune recognition molecules in a number of invertebrates, which operate by binding unusual carbohydrate residues associated with pathogens and parasites [39]. Larval schistosomes may share glycans with the host haemocytes, leaving these cells unable to recognise the presence of such parasites via lectin-based recognition systems [37,38]. Probing for the presence of shared surface carbohydrates between parasites and haemocytes using biotinylated lectins has proven to be a useful and rapid initial approach to look for their role in molecular mimicry [39]. Such approaches may be a useful first point of investigation in the crustacean–Hematodinium model.

Conclusion

In this Opinion article, we have reviewed and discussed evidence that gives new insight into the host relationship of this key dinoflagellate parasite. This assemblage, belonging to the genus Hematodinium, has the capacity to successfully invade and multiply in the tissues of many different crustacean hosts without eliciting any obvious cellular immune response such as parasite encapsulation. There are very few parasites or pathogens that have the ability to circumvent the cellular defence reactions of several different hosts, making this of wide interest to immunologists. Whatever the mechanism(s) employed, these parasites have emerged as a significant threat to the future sustainability of shellfish fisheries and aquaculture worldwide, and hence, they warrant research focus.

Acknowledgments

We are particularly grateful to Dr. Hamish J. Small (Virginia Institute of Marine Science) for his constructive criticism of a preliminary version of the manuscript and for sharing with us some of his unpublished observations.

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