The infectivity of rotavirus, the main causative agent of childhood diarrhea, is dependent on activation of the extracellular viral particles by trypsin-like proteases in the host intestinal lumen. This step entails proteolytic cleavage of the VP4 spike protein into its mature products, VP8* and VP5*. Previous cryo-electron microscopy (cryo-EM) analysis of trypsin-activated particles showed well-resolved spikes, although no density was identified for the spikes in uncleaved particles; these data suggested that trypsin activation triggers important conformational changes that give rise to the rigid, entry-competent spike. The nature of these structural changes is not well understood, due to lack of data relative to the uncleaved spike structure. Here we used cryo-EM and cryo-electron tomography (cryo-ET) to characterize the structure of the uncleaved virion in two model rotavirus strains. Cryo-EM three-dimensional reconstruction of uncleaved virions showed spikes with a structure compatible with the atomic model of the cleaved spike, and indistinguishable from that of digested particles. Cryo-ET and subvolume average, combined with classification methods, resolved the presence of non-icosahedral structures, providing a model for the complete structure of the uncleaved spike. Despite the similar rigid structure observed for uncleaved and cleaved particles, trypsin activation is necessary for successful infection. These observations suggest that the spike precursor protein must be proteolytically processed, not to achieve a rigid conformation, but to allow the conformational changes that drive virus entry.
Rotavirus is responsible for more than 400,000 annual infant deaths worldwide. Its viral particle bears 60 protuberant spikes that constitute the machinery responsible for virus binding to and entry into the host cell. For efficient infection, the protein molecules that build the spike must be cleaved. Despite the importance of this activation step, the nature of the changes induced in the spike structure is unknown. According to the current hypothesis, the uncleaved spike is very flexible, and activation stabilizes the spike in an entry-competent conformation. Here we used distinct electron microscopy techniques to determine the structure of the uncleaved particle in two model rotavirus strains. Our results provide a complete structure of the uncleaved spike and demonstrate that cleaved and uncleaved spikes have similar conformations, indicating that proteolytic processing is not involved in stabilization of the spike. We suggest that spike processing is important for infection since it is necessary to allow the spike domain movements involved in rotavirus entry.
Citation: Rodríguez JM, Chichón FJ, Martín-Forero E, González-Camacho F, Carrascosa JL, Castón JR, et al. (2014) New Insights into Rotavirus Entry Machinery: Stabilization of Rotavirus Spike Conformation Is Independent of Trypsin Cleavage. PLoS Pathog 10(5): e1004157. https://doi.org/10.1371/journal.ppat.1004157
Editor: Félix A. Rey, Institut Pasteur, France
Received: February 15, 2014; Accepted: April 16, 2014; Published: May 29, 2014
Copyright: © 2014 Rodríguez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants from the Funds for Health Research (FIS) from the Spanish Ministry of Economy and Competitivity (AES/FIS, PI08/0688 to JMR and AES/FIS, PI10/00895 to DL), and BFU2011-29038 (to JLC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
To initiate infection, viruses must overcome the complex membranous system that surrounds and resides within the cell. The ability of the virus to penetrate this barrier is one of the elements that define virulence and host range. Entry into the host cell is thus a key factor in viral infectivity, and a natural target for the design of efficient strategies against virus infections .
Rotaviruses are non-enveloped, double-stranded (ds)RNA viruses of the Reoviridae family; they infect only vertebrates, via the oral-fecal route. Their replication is generally limited to terminally differentiated enterocytes of the intestinal tract, with severe gastroenteritis restricted in the great majority of cases to the young . In humans, rotavirus infection is the leading cause of medical gastroenteritis in children under five years of age , .
The rotavirus mature virion is a complex triple-layered particle (TLP) built around its inner capsid, a T = 1 icosahedral shell made of 60 asymmetric dimers of the VP2 protein , . Inside this core, each of the eleven dsRNA segments of the viral genome is associated, below the five-fold symmetry axes, with one copy of the RNA-dependent RNA polymerase VP1, and the RNA capping enzyme VP3 , . The inner core is surrounded by a thick shell formed by 260 trimers of the VP6 protein ordered in an icosahedral T = 13 symmetry , , . This double-layered particle (DLP) constitutes the rotavirus transcriptional machinery and, characteristically of Reoviridae family members, it does not disassemble during viral infection. Rotavirus infection is effectively initiated when the DLP is released into the cytoplasm and begins synthesis of the viral transcripts.
The DLP are not infective, however, as they lack the ability to identify, bind and penetrate target cells; those functions reside in the external layer of the mature TLP , . This external shell is formed by 260 trimers of the VP7 glycoprotein, ordered in a T = 13 icosahedral lattice. Each VP7 trimer rests on one of the VP6 trimers of the underlying DLP, anchored to small protrusions of the VP6 layer by its flexible N-terminal arm , . Sixty spikes, formed by trimers of the VP4 protein, project from the VP7 shell. They are anchored in depressions in the VP6 layer that surround the five-fold axes, clamped by the VP7 shell that partially covers their base .
To become fully infectious, cell-released TLP must be digested by trypsin-like proteases from the intestinal lumen , . This activation step cleaves the VP4 protein after three defined sites (Arg231, Arg241 and Arg247) to produce two main fragments, the N-terminal VP8* and the larger C-terminal VP5*. Proteolytic processing of the spikes is thought to occur through an ordered cleavage cascade that culminates in scission at Arg247. Cleavage after this residue is essential for membrane interactions and infectivity , .
Analysis of the near-atomic structure of the cleaved TLP shows that the three VP4 molecules that form the spikes, termed VP4A-C , are organized into a complex structure that is held in place by non-covalent interactions among its components (Figure 1). Structural and biochemical data have allowed formulation of a model for rotavirus entry in which VP4 plays a role similar to that of fusion proteins during enveloped virus entry , –. Receptor binding and attachment take place through the distal lectin domains of the two VP8* molecules of chains A and B. Probably triggered by this binding, the spike components are reorganized into an extended intermediate in which hydrophobic loops of the three VP5* β-barrels, previously covered by the lectin domains (chains A and B) or by the spike body (chain C), are inserted into the target cell membrane. An additional unknown triggering event would provoke the transition from this extended intermediate to a folded-back structure, in which the hydrophobic loops now point toward the virus particle. The remarkable similarity of the initial form, the extended intermediate, and the folded-back conformation to analogous structures involved in membrane fusion of enveloped viruses suggests that the energy released by these vast conformational changes is used by rotaviruses to disrupt the cell membrane.
Ribbon representation (top) of the atomic structure of the VP4 spike (PDB entry 3IYU) color-coded to represent the spike foot (green), stalk (red), body (red) and head (purple). As indicated (VP4-A, -B and -C), each of the subunits is highlighted in color, while the remaining molecules are shown in grey. The amino- and carboxy-terminal regions of the three molecules, located contacting one another beneath the VP7 layer , contribute equally to the trimeric foot of the structure. The asymmetric central stalk is formed mainly by the VP5* β-barrel domain of VP4-C, which lies almost parallel to the particle surface. The distal part of the spike, constructed by equal contributions of the A and B molecules, contains the body (built by the VP5* β-barrel domains) and the globular heads (formed by VP8* lectin domains). The unusual structure of VP4-C appears to arise from asymmetries on the VP7 trimers that surround the base of the spike, which allows for distinct interactions with the base of the VP5*-C β-barrel. The globular VP8* lectin domain of the VP4-C molecule is not accounted for in the resolved structure, and is presumed to be released during proteolysis. Diagram of the organization of the VP4 chain (bottom), color-coded as in top. The VP4 proteolytic products (VP5* and VP8*) and domains are labeled. Residues delimiting domains and trypsin cleavage sites are indicated.
In this model, as with the fusion proteins of some enveloped viruses, proteolytic cleavage of the rotavirus spike protein VP4 into VP8* and VP5* primes rotavirus TLP for efficient infectivity , . Despite the availability of the atomic structure of the trypsinized rotavirus TLP, understanding of the structural changes involved in this process has been hampered by the lack of information regarding the structure of the undigested spike. Available single particle cryo-electron microscopy (cryo-EM) reconstructions of undigested TLP show no density for VP4 projecting from the VP7 shell . This suggests that proteolytic processing of VP4 triggers undetermined structural changes in the spike that result in a more stable, rigid spike structure, as described by the atomic model, that mediates rotavirus entry.
Here we used cryo-EM and cryo-ET to study the structure of the undigested rotavirus spike. Cryo-EM results showed that the structure of the uncleaved TLP of rotavirus strains SA11 and OSU is indistinguishable from that of the trypsin-digested particle, and concurs with the previously resolved near-atomic structure of the mature TLP. Cryo-ET analyses provided new insight into the organization of the uncleaved spike, and a model for its complete structure.
Purification and characterization of TLP
Rotavirus TLP were purified from cells infected with rotavirus strain SA11, in the presence of trypsin (TR-TLP), or in its absence in medium supplemented with the protease inhibitor leupeptin (NTR-TLP). Purified particles were characterized by Coomassie blue staining of SDS-PAGE gels and by western blot analysis using an antibody specific for protein VP4/VP5*. In NTR-TLP samples, the VP4 spike protein was detected mainly as the 98 kDa unprocessed precursor form (Figure 2A, S1), whereas in TR-TLP, the trypsin proteolytic products VP8* (28 kDa) and VP5* (55 kDa) account for most of the VP4 protein mass (Figure 2B, S1). During virus purification, TLP spike stability depends both on viral strain and the proteolytic state of its VP4 components . To estimate the amount of spike protein preserved in our preparations, we quantified VP4 (in NTR-TLP) or its product VP5* (in TR-TLP) relative to protein VP6. Densitometric analysis of the Coomassie-stained gels produced values of 70±3% and 85±6% of the stoichiometric amount for NTR- and TR-TLP, respectively, which indicates that most spike protein was maintained to a similar extent in both samples.
(A, B) Coomassie blue-stained SDS-PAGE gels of purified SA11 TLP grown in the absence (A) or presence (B) of trypsin. Positions of rotavirus structural proteins (VP) are indicated. Unprocessed spike protein VP4 and its proteolytic products VP8* and VP5* are highlighted in grey. (C, D) Cryo-electron micrographs of NTR (C) and TR (D) particles. Arrowheads indicate examples of clearly defined spikes projecting from the virion surface. The bar represents 50 nm.
Cryo-EM analysis of NTR- and TR-TLP (Figure 2C, D) showed a homogeneous population of well-preserved rotavirus particles; in some, VP4 (or VP8*/VP5*) spikes can be visualized projecting from the virion surface (Figure 2C, D, arrowheads).
Single particle analysis of NTR- and TR-TLP
Three-dimensional reconstructions (3DR) were calculated for NTR- and TR-TLP with a resolution of 12.8 Å and 11.9 Å, respectively (Figure 3), obtained at a Fourier shell correlation (FSC) threshold of 0.3 (Figure S2). At the resolution achieved, the 3DR for NTR-TLP (Figure 3A–C) and TR-TLP (Figure 3D–F) were virtually indistinguishable; a difference map calculated between them showed no significant differences. The molecular architecture of the spike in both particles (Figure 3C, F) is consistent with previous structural studies for TR-TLP , , in which the cleaved spike adopts a distinctive, rigid bilobulate shape divided into a head, body, stalk and foot domains  (Figure 1, S3). Comparison of the relative density of spikes in both density maps using VP2-VP6-VP7 shell density as a reference (Figure 3B, E; arrowheads) showed an equivalent occupancy level (58 and 50% for NTR- and TR-TLP, respectively).
(A, D) Surface-shaded representation of the outer surfaces of NTR (A) and TR (D) particles, viewed along an icosahedral 2-fold axis. The surfaces are radially color-coded to represent VP4 or VP8*/VP5* spikes (red), VP7 (yellow), and VP6 (blue). The density is contoured at 1σ above the mean. The bar represents 100 Å. (B, E) Transverse sections, 2.8 Å thick, taken from the maps of NTR- (B) and TR-TLP (E), parallel but displaced 34 Å from the central section, viewed along a 2-fold axis (darker, denser). Arrows indicate spikes in the surface-shaded representations in A and D and their corresponding densities in B and E. For the NTR map, the relative density of the spike contained ∼50% of the shell density; for the TR map, relative density was ∼55%. (C, F) Close up view of the NTR (C) and TR (F) spike represented as in A and D.
The almost identical structure observed for NTR and TR spikes (Figure 3C, F) contrasts with a previous report for the SA11-4F rotavirus strain, in which the spikes were resolved in the cryo-EM 3DR of the TR-TLP, but were undetectable for undigested TLP . This difference prompted us to verify whether the presence of the protease inhibitor leupeptin or the purification method affected our results. To exclude these possibilities, NTR-TLP were produced in the absence of leupeptin, following the purification method described previously . Analysis of these capsids produced a virion map equivalent to previous NTR- and TR-TLP (Figure S2 and S4). To ascertain whether these differences are strain-specific, NTR- and TR-TLP were produced from the porcine strain OSU  and analyzed (Figure S5, S6, S7). Cryo-EM 3DR of OSU NTR- and TR-TLP are essentially indistinguishable from each other. There were no significant differences between the OSU strain density maps or when they were compared with the 3DR of the SA11 particles.
These results show that spikes formed by trimers of undigested VP4 have a conformation similar to that of the trypsin-digested entry-primed rotavirus particles. For the simian and porcine strains analyzed in this study, ordering of the VP4 spike in a rigid bilobulate conformation is independent of trypsin digestion.
Infectivity assays of NTR- and TR-TLP
To study the effect of trypsin cleavage on infectivity, we determined the specific infectivity of both TLP types, before and after trypsin treatment (Figure 4). In vitro trypsin treatment of NTR-TLP resulted in disappearance of the VP4 band and appearance of proteolytic products VP8* and VP5*; there were no detectable changes in the protein profile following similar treatment of TR-TLP (Figure 4A, B, grey arrows). Due to the trypsin dependence for rotavirus plaque formation, the specific infectivity of mock-treated TLP was evaluated in a focus-forming assay in the absence of trypsin, in which expression of the viral protein NSP4 was used to detect infected cells (Figure 4C, D). The background infectivity in NTR-TLP preparations is attributed mainly to the activity of proteases released during cell lysis . Undigested NTR-TLP had ∼1.5 logarithmic units lower specific infectivity than undigested TR-TLP (1.89 for SA11, p<0.02; 1.56 for OSU, p<0.005). The specific infectivity of trypsin-treated TLP was determined in a plaque-forming assay that directly tests for infective particles able produce infective progeny. In this assay, trypsin-activated NTR- and TR-TLP showed specific infectivity levels with no significant differences (Figure 4E, F). The results demonstrate that trypsin treatment enhances NTR-TLP specific infectivity to a level similar to that of trypsin-activated TR-TLP, as shown for several rotavirus strains , . Although we detected no structural differences between NTR- and TR-TLP by single particle cryo-EM 3DR, proteolytic processing of VP4 is necessary for efficient viral infectivity.
(A, B) Coomassie blue-stained SDS-PAGE gels of purified SA11 (A) and OSU (B) TLP grown in the absence (NTR) or presence (TR) of trypsin. Samples were mock-incubated (-Trp) or incubated in vitro with 100 BAEE units/ml of trypsin (+Trp) (30 min, 37°C). The positions of the structural proteins (VP) are indicated. The unprocessed spike protein VP4 and its proteolytic products VP8* and VP5* are highlighted in grey. (C, D) Determination of specific infectivity of SA11 (C) and OSU (D) TLP by fluorescent focus assay in the absence of trypsin. (E, F) Determination of infectivity of SA11 (E) and OSU (F) TLP by plaque-forming assay in the presence of trypsin. Data are shown as mean ± SD. FFU, focus-forming units. PFU, plaque-forming units. * p<0.02, ** p<0.005.
Cryo-electron tomography of NTR- and TR-TLP
The densities for the spikes in single particle cryo-EM analysis of NTR- and TR-TLP can be assigned to most of the three VP4 polypeptide chains (A, B and C) that compose the spikes, with the exception of the lectin domain of the VP4-C molecule (Figure 1). It is suggested that this lectin domain is lost during TR-TLP preparation . In NTR spikes, it is covalently bound to the rest of the VP4 molecule, which indicates that the density for this domain is smeared due to the icosahedral averaging imposed during 3DR. We used cryo-ET to overcome this limitation and study the structure of SA11 strain NTR-TLP, a strategy used successfully to analyze surface proteins of enveloped viruses and non-symmetric elements in icosahedral viruses , . We collected several cryo-tomographic series of projection images of SA11 NTR- and TR-TLP covering the angular range from −66° to +66°. Tomograms from these tilt-series were reconstructed to obtain the averaged map of 607 and 242 single NTR and TR particles, respectively (Figure 5A–D).
(A, C) Slice through the xy plane of the reconstructed cryo-electron tomograms of NTR- (A) and TR-TLP (C). The bar represents 100 nm. White arrowheads indicate examples of electrodense structures inside particles. Black arrowheads indicate examples of spikes on the outer particle surface of the virions. (B, D) Gallery of central slices through extracted NTR- (B) and TR-TLP (D). (E, G) Surface-rendered model of the averaged NTR- (E) and TR-TLP (G) calculated from the extracted subtomograms and viewed along an icosahedral 2-fold axis. The bar represents 100 Å. (F, H) Close up view of the NTR (C) and TR (F) spike represented as in E and G.
Virtual sections from individual particles showed an electron-dense region near the geometric center of some particles (∼30% and ∼15% of NTR and TR, respectively, Figure 5A, C, white arrowheads), which can only be associated with the genomic dsRNA. This structure is reminiscent of the dsRNA condensation detected by cryo-EM analysis of TR-TLP in the presence of ammonium ions at high pH , although the biological importance of this feature is not clear. Projecting densities that correspond to the virion spikes were clearly visible on the outer particle surface of both samples (Figure 5A, C, black arrowheads).
Subtomograms of individual virions were extracted (Figure 5B, D), aligned and averaged, considering icosahedral symmetry to optimize determination of the origin and orientation of each virion subvolume. In accordance with our results in single particle cryo-EM 3DR (Figure 3), the spikes in the final averaged density maps of the NTR- and TR-TLP showed no significant differences (Figure 5).
This alignment process not only generated an average structure for the virions, but also allowed determination of the origin and orientation of each particle subvolume relative to the average density. We used this information, combined with knowledge of each spike position in the average structure and of their icosahedral symmetry relationships, to computationally extract and orient 36,420 and 14,580 spike subtomograms from the original non-symmetrized densities of the NTR- and TR-TLP, respectively. The extracted spike subtomograms were then reference-free classified using a maximum-likelihood algorithm that takes into account the missing wedge information . In this process, no spike density was detected projecting from the VP7 shell for 28% of the NTR and 40% of the TR spike subtomograms, which could correspond to positions where spikes have been lost and those in which they are flexible or disordered. For the remaining subtomograms, the classification process converged to two classes for the subtomograms of the NTR spikes, whereas there was only one class in the TR spike subtomograms (Figure 6A, B). Positions in which no density was detected and those assigned to a class were distributed randomly at the virus particle surface.
(A, B) Surface-rendered model of averaged tomograms, reference-free classified, for NTR (A) and TR (B) spikes. Top and middle rows show two side views of the averages related by a 90 degree rotation. The bottom row shows the top view of the averages. Arrowheads indicate an extra density at the base of the spike stalk in the class 2 NTR average, which is absent in NTR and TR class 1 averages. (C) Surface-rendered model of the class 1 TR spike fitted with the VP4 atomic model (PDB entry 3IYU). VP8* molecules A and B are in purple, VP5* molecules A and B are in red, and VP5* C is in orange. The last resolved residue of VP8* (Lys29, purple) and the first resolved residue of VP5* (Glu264, orange) are indicated by spheres for the VP4-C molecule. (D) Surface-rendered model of the class 2 NTR spike fitted with the VP4 atomic model and represented as in C. (E) Close up view of the NTR spike fitted with a single VP8* lectin domain superimposed on the extra density detected at the base of the stalk. First (Leu65, cyan) and last (Leu224, yellow) residues for the fitted domain are represented as spheres (arrowheads).
Class 1 contains the majority of the NTR and TR spike subtomograms (47% for NTR, 60% for TR) and yielded averages that greatly resemble the structure of the NTR- and TR-TLP spikes obtained when icosahedral symmetry was imposed on the 3DR from cryo-EM (Figure 3) or cryo-ET data (Figure 5). Fitting of the atomic coordinates of trypsinized VP4  into class 1 averages showed good agreement for the spike stalk, body and head (Figure 6C). The lectin domain of the VP4-C molecule is the only VP4 region not accounted for by either the atomic model or the density maps. Although for the TR spike it could be argued that this lectin domain is lost after proteolytic cleavage, the existence of this class for the NTR subtomograms, in which the lectin domain is covalently bound to the remainder of the VP4-C molecule, indicates that this domain is more flexible in this molecule than the equivalent domain of the other VP4 molecules. Class 2 was detected only for NTR spikes, and contained 25% of the subtomograms. The averaged spike density is similar to that of class 1, with an additional bulge at the base of the spike stalk (Figure 6A, arrowheads). When the atomic coordinates of the TR VP4 (Figure 6D) were fitted to this averaged volume, the unassigned bulge density was compatible in size and shape with a single lectin domain (Figure 6E). The terminal residues of this domain (Leu65 and Leu224, cyan and yellow spheres, respectively, in Figure 6E) were located just above the last defined residues in the atomic model for the VP4-C flanking regions (Lys29 and Glu264, purple and orange spheres, respectively, in Figure 6C–E) . The evidence suggests that this density corresponds to the lectin domain of the VP4-C molecule that contributes to the spike stalk, and that the average density for class 2 subtomograms reflects the complete structure of the undigested rotavirus spike.
Recent reports suggest that the conformational changes undergone by the rotavirus spike protein VP4 are the main driving force behind membrane disruption and virus entry into the host cell , , . For this process to be efficient, the rotavirus particle must be primed by proteolytic cleavage of VP4 into its mature products, VP8* and VP5*. Despite the availability of an atomic structure for the trypsinized rotavirus particle, our understanding of the molecular mechanisms that underlie the proteolytic enhancement of rotavirus infectivity has been hindered by the lack of a structure of the undigested spike. The aim of this study was to determine, using cryo-EM and cryo-ET, the structure of the uncleaved rotavirus to improve comprehension of the structural mechanisms underlying the proteolytic enhancement of rotavirus infectivity.
Single particle cryo-EM reconstructions of the NTR- and TR-TLP of the simian SA11 and porcine OSU rotavirus strains yielded structures that showed no differences among them and were consistent with the near-atomic structure of the rotavirus RRV strain TR-TLP . This indicates that the overall spike conformation achieved by the three complete undigested VP4 molecules is essentially maintained after trypsin activation. The interactions of the unprocessed VP4 molecules with the VP6 and VP7 trimers during assembly in the six-coordinated cavity are thus sufficient to configure these molecules in the A, B or C conformations, according to their relative position. This structure for the undigested spike is consistent with the available structural data for the TR-TLP particle, since the length of the trypsin-released segment of the loops is sufficient to bridge the observed C and N termini of VP8* and VP5*, respectively . This architecture would also explain the differential trypsin sensitivity of unassembled and spike-assembled VP4 , as most putative trypsin-sensitive sites are protected in the latter, with the exception of the known cleavage sites Arg230, Arg241, Arg247 and the proposed cleavage site at Lys29 in VP8* of VP4-C . The observation of an equivalent structure at the cell interaction domain of cleaved and uncleaved spikes is also consistent with the finding that both particle types bind similarly to cell membranes .
Although trypsin cleavage has only a limited effect on spike structure, infectivity experiments showed that protease cleavage of the spike protein is essential for high levels of specific viral infectivity. These data suggest that whereas the fold shared by NTR and TR particles is attachment-competent, the spike must be primed by cleavage for further conformational changes. In this dependence on proteolytic activation, as well as in the nature of the conformational changes during viral entry, VP4 has a striking similarity to some membrane fusion proteins of enveloped viruses , . The class 1 viral fusion glycoproteins are synthesized as precursors that require protease activation for virus infectivity , . This necessary activation step is associated with relatively little structural rearrangement of the fusion protein, similar to our observations for rotavirus. For example, the atomic structures of the uncleaved and cleaved forms of the influenza prefusion hemagglutinin protein are largely superimposable, except for the 19-residue segment that includes the protease cleavage site , . In the uncleaved form, these residues are in an exposed loop; after cleavage, the fusion peptide in the newly-formed N terminus is buried in a nearby hydrophobic cavity. In the paramyxovirus parainfluenza virus 5, structural movements between the cleaved and uncleaved forms of the fusion protein are also limited to the residues that compose and surround the protease recognition site; since the residues that compose the hydrophobic fusion peptide are already packed in the uncleaved structure, the conformational changes are even more subtle .
The cryo-EM-derived atomic structure of the rotavirus TR-TLP does not account for the lectin domain of VP4-C . Results were similar for the NTR- and TR-TLP 3DR of both strains used in this study. The absence of density for this domain, which in NTR-TLP is covalently bound to the rest of the VP4-C subunit, indicates greater flexibility of this part of the molecule, resulting in loss of the density due to the icosahedral averaging imposed during 3DR. The classification of cryo-ET subvolumes allowed us to overcome this limitation, and the averages for the distinct classes provides a deeper understanding of the spike structure.
The atomic model of the TR-TLP spike was fitted with good agreement to the averaged density of class 1, the most abundant class for NTR-TLP spikes and the only class detected in TR-TLP spikes. Neither the averaged maps for class 1 nor the atomic coordinates detect the VP4-C lectin domain. This domain is also absent in all 3DR derived from cryo-EM single particle analysis, as well as the in the icosahedrally averaged maps of virion volumes obtained by cryo-ET. In the case of NTR-TLP, this domain is covalently linked to the remainder of the VP4-C molecule; classes 1 and 2 thus contain three complete VP4 molecules in NTR samples. Nonetheless, we only identified a density compatible with this domain in the class 2 averaged maps, observed only in NTR-TLP subtomograms. This apparent contradiction could be a result of VP4-C lectin domain flexibility. As illustrated in Figure 7, spike subtomograms in which this domain is located near a central position (25%, white asterisk in Figure 7, top) generate the class 2 averaged density. Class 1 subvolumes (47%) are composed of densities in which the lectin domain swings away from this central position (black asterisks in Figure 7, top) and whose averaging results in disappearance of the density. In the case of the spike subtomograms from TR-TLP, the lack of a class equivalent to class 2 might indicate that trypsin digestion further increases lectin domain flexibility or, as suggested by analysis of the TR-TLP atomic structure, that a second cleavage in Lys29 releases the domain from the spike (Figure 7, bottom) .
NTR spikes have a flexible VP4-C lectin domain at the base of the stalk (top, left). NTR Class 1 represents the average of more distal positions (black asterisks) and class 2 is generated by the average of more central positions (white asterisk) of the flexible lectin domain. The proteolytic processing of spike components releases the VP4-C lectin domain (if an additional cleavage at Lys29 is produced) or increases its flexibility in TR class 1 spikes (bottom, left). The fraction of NTR (top, right) and TR (bottom, left) subvolumes in which no spike density is detected could correspond to a mixture of positions without spikes (or with damaged spikes) and with highly flexible spikes.
In both samples, a fraction of the subtomograms (28% of NTR and 40% of TR) rendered averaged maps that show no density in the regions in which the spikes are located (Figure 7, No Spike density). These subtomograms would include positions in which the spike is very flexible, has been lost, or its structure has been damaged due to the purification conditions (particularly the organic extraction procedure ). Biochemical analysis of the TLP preparations (Figure 2) shows that a percentage of the predicted VP4 molecules is not present in the purified NTR (30%) or TR (15%) particles. Although we cannot rule out the possibility that all volumes in these fractions correspond to positions at which the spike has been lost, the large percentage of subtomograms included in this group, especially in the case of TR-TLP positions, suggests that they also include positions in which the spike is present but is highly flexible and whose density is lost after averaging (Figure 7). It can also be argued that the greater percentage of tomograms in this fraction in TR-TLP reflects a greater degree of freedom induced by trypsin cleavage of VP4 into its products, VP8* and VP5*. The redundancy derived from the virion icosahedral symmetry could explain its tolerance for the presence of defective spikes in the particle. Indeed, the relatively low occupancy observed in in vitro recoated particles is sufficient for a very efficient infectivity .
Previous cryo-EM single particle analysis of the NTR-TLP structure did not detect a density corresponding to the spike structure . These different results could be attributed to the nature of the SA11-4F strain used in the previous 3DR, which is unique in its ability to produce small clear plaques in the absence of trypsin, and for its extreme sensitivity to protease digestion . These characteristics arose from the reassortment of a segment 4 that encodes a VP4 gene from a bovine rotavirus on a SA11 genetic background , . The molecular basis of the distinct behavior of SA11 and SA11-4F strains is thus probably due to changes in the sequence of the reassorted VP4 molecule (129 changes in a total of 776 residues). The large number of variations makes it impossible to determine the amino acids involved. Nonetheless, the VP4 region that interacts with VP6, which mediates spike foot recruitment and trimerization, is conserved. There are several changes in the VP7-contacting region of VP4, which dictates how the spike projects, as well as in the preceding linker, which is not resolved in the atomic structure . The SA11 and OSU strains used in this study show more standard behavior, with no evidence of interspecies reassortment. Based on our results, we hypothesize that whereas most NTR-TLP spikes in SA11 and OSU strains are stabilized, the majority of VP4 molecules in the SA11-4F strain are not stabilized, and the spike density is lost when it is averaged and icosahedral symmetry is imposed. We suggest that the structures we observed for SA11 and OSU uncleaved spikes represent a more general architecture, and that SA11-4F is an extreme example of uncleaved spike flexibility.
In summary, our cryo-EM studies of two unrelated model rotavirus strains show that in the absence of trypsin cleavage, most of the three VP4 molecules that compose the spike adopt a stable conformation, similar to that of the mature cleaved TLP. Cryo-ET results reinforce this conclusion and, additionally, evidence the great flexibility of the lectin domain of the VP4-C chain, providing us with a model for the complete structure of the uncleaved spike. Our findings indicate that cleavage of the spike proteins is important for infectivity because it influences later events, probably those conformational changes proposed to mediate membrane disruption.
Materials and Methods
Virus production, purification and titration
The monkey epithelial cell line MA104 (ECACC 85102918) was cultured in MEM with 10% fetal calf serum and used between passages 7 and 24. The simian agent 11 rotavirus strain (SA11, , ) was obtained from Dr. J. Buesa, (University of Valencia, Valencia, Spain), and the Ohio State University porcine strain (OSU; ) from Dr. O. Burrone (ICGEB, Trieste, Italy). Both strains were cloned by four successive plaque isolation steps in MA104 cells. The clones SA11-C4111 and OSU-C5111 were selected and amplified. cDNAs of the complete genome segments of the two viral clones were obtained following procedures described by Potgieter et al. , cloned in the plasmid pJET1.2 (Fermentas) and sequenced. Given the complex history of the SA11 family of viruses , , we analyzed the genome sequence of the SA11-C4111 strain. The results showed a SA11-like genome very similar to that of the N5 strain , with no evidence of genomic reassortment. The amplified viruses were used within three passages of the last plaque isolation step. For simplicity, here we refer to these clones as SA11 and OSU.
TLP were produced and purified by ultracentrifugation in CsCl gradients as described by Patton et al. , with minor modifications (see supporting Text S1). In parallel experiments, aliquots of the TLP samples were vitrified (below), treated with SDS-PAGE sample buffer for biochemical characterization or analyzed for infectivity. Viral infectivity was determined by plaque assays and fluorescent focus assays, essentially as described by Arnold et al.  with minor modifications (see supporting Text S1).
NTR and TR-TLP samples (5 µl) were applied to one side of acetone-washed Quantifoil R 2/2 holey grids, blotted, and plunged into liquid ethane using a Leica EM CPC cryo-fixation unit. Cryo-EM images were recorded in low-dose conditions (∼10 e−/Å2), in a Tecnai G2 electron microscope operating at 200 kV. For SA11 NTR and TR samples, micrographs were recorded at a nominal magnification of 50,000X. SA11 NTR-TLP produced in the absence of leupeptin and OSU samples were imaged on a FEI Eagle 4k CCD at a detector magnification of 67,873X (2.21 Å/pixel sampling rate).
General image processing operations were performed using Bsoft  and Xmipp  software packages. Graphic representations were produced by UCSF Chimera . Micrographs were digitized using a Nikon Super CoolScan 9000 ED at a 6.35 µm step size, or a Zeiss TD scanner at a 7 µm step size, to yield 1.27 Å or 1.4 Å pixel size in the specimen, respectively. X3d  was used to manually select 3,802, 2,980, 2,150, 4,100 and 2,100 individual images for SA11 NTR-, SA11 TR-, SA11 NTR - leupeptin, OSU NTR- and OSU TR-TLP, respectively. Defocus was double determined with bshow  and CTFfind , and the CTF were corrected in the images by flipping phases in the required lobes. The published structure of the rotavirus VP7-recoated particle , low-pass filtered to 30 Å, was used for the initial determination of the origin and orientation of each particle for all samples. As these recoated particles lack VP4, any model bias at the spike density is avoided. Xmipp iterative projection matching was carried out to determine and refine the origin and orientation of each particle. Reconstructions were computed using interpolation in Fourier space. After each iteration, resolution was assessed by FSC, applying a correlation limit of 0.5 between two independent reconstructions. The final reconstructions combined 3,421 images for SA11 NTR, 2,682 for SA11 TR, 1,935 for SA11 NTR - leupeptin, 3,690 for OSU NTR and 1,890 for OSU TR. Amplitude decay was corrected by adjusting the spatial frequency components of the cryo-EM maps to the decay profile of the atomic map of rotavirus TLP (PDBs 3N09 and 2GH8). This adjustment was applied in the frequency range from 340 Å to the maximum resolution achieved, and a soft low-pass filter was applied. Amplitude decay was also calculated and corrected with Embfactor , with similar results.
Samples were mixed with 10 nm gold particles and vitrified as described above. Tomographic tilt-series were recorded in a Tecnai G2 electron microscope operating at 200 kV on a FEI Eagle 4k CCD using the FEI Xplore3D software at a detector magnification of 32,609X (4.6 Å/pixel sampling rate) every 1.5 degrees. Images were acquired with a defocus ranging from 5 to 8 µm, and an accumulated total dose from 90 to 120 e/Å. Tilted series were processed using IMOD  and CTF corrected using TOMOCTF . A final number of 4 and 3 tomograms were reconstructed for SA11 NTR and TR samples, respectively, using unfiltered weighted back projection algorithms implemented in the TOMO3D package  to recover all the high frequencies for subvolume averaging process.
Subtomogram averaging and classification
IMOD software was used to manually select, and extract 607 and 243 virions from SA11 NTR and TR samples, respectively. Subvolumes were aligned and averaged using the MLTomo routine  from Xmipp, considering icosahedral symmetry to optimize the origin and orientation determination at this step. This process was performed using the previously obtained cryo-EM 3DR as initial template, as well as in a reference-free manner. Both approaches yielded equivalent results. The final averaged volumes were used to determine the 3D position of the spikes. This information, together with the origin and orientation assigned to each particle, were used to automatically extract 36,420 and 14,520 subvolumes for each spike in the original asymmetric tomograms of SA11 NTR and TR, respectively. The spike subtomograms were classified and averaged with MLTomo. Independently of the initial number of classes used, the classification of the NTR spikes converged to 3 groups containing 10,084 (28%, no spike density), 17,281 (47%, class 1) and 9,055 (25%, class 2) volumes, whereas classification of the SA11 TR spikes converged to two groups with 5,810 (40%, no spike density) and 8,710 (60%, class 1) volumes.
Western blot analysis of SA11 and OSU NTR- and TR-TLP. (A) Coomassie blue-stained SDS-PAGE gel of purified SA11 and OSU NTR- and TR-TLP. Positions of structural viral proteins (VP) are indicated. Position of unprocessed spike protein VP4, and its products VP5* and VP8* are highlighted (grey). (B) Western blot analysis. A gel similar to that in A was immunoblotted with an anti-VP4 antibody that recognizes the precursor VP4 and its product VP5*.
Assessment of the resolution cryo-EM 3DR of SA11 NTR and TR TLP. (A) FSC resolution curves were calculated for SA11 NTR-TLP (blue, continuous line), NTR-TLP grown in absence of leupeptin (purple, dashed line) and TR-TLP (blue, dashed line). For the 0.5 threshold the values for SA11 NTR-, TR- and NTR-TLP without leupeptin were 15.4, 14.5 and 15.9 Å, respectively; values for the 0.3 threshold were 12.8, 11.9 and 13.3 Å, respectively.
Fit of the atomic coordinates into the cryo-EM map. (A) Scheme of the NTR spike and its interaction with VP6 and VP7 shells. Proteins and color coding are indicated. (B) Superposition of the atomic coordinates of RRV TR-TLP (PDBs 3N09 and 2GH8)  and the SA11 NTR-TLP cryo-EM map. Coordinates and densities are color-coded as in A.
Biochemical and structural analysis of SA11 NTR-TLP grown in the absence of leupeptin. (A) Coomassie blue-stained SDS-PAGE gels of purified SA11 TLP grown in the absence of trypsin and leupeptin. Positions of structural viral proteins (VP) are indicated. Position of unprocessed spike protein VP4 is highlighted in grey. (B) Cryo-electron micrograph of vitrified particles. The bar represents 50 nm. (C) Surface-shaded representation of the outer surface of the 3DR, viewed along an icosahedral 2-fold axis. The surface is radially color-coded to represent VP4 (red), VP7 (yellow) and VP6 (blue). The density is contoured at 1 σ above the mean. (D) Transverse sections, 2.8 Å thick, taken from the maps parallel but displaced 34 Å from the central section, viewed along a 2-fold axis (darker, denser). Arrows indicate spikes in the surface-shaded representations in C and their corresponding densities in D. (E) Close-up view of the spike represented as in C.
Analysis of OSU NTR- and TR-TLP by SDS-PAGE and cryo-EM. (A, C) Coomassie blue-stained SDS-PAGE gels of purified OSU TLP grown in the absence (A) or presence (C) of trypsin. Positions of structural viral proteins (VP) are indicated. Unprocessed spike protein VP4 and its proteolytic products VP8* and VP5* are highlighted in grey. (B, D) Cryo-electron micrographs of NTR (B) and TR (D) particles. The bar represents 50 nm.
Assessment of the resolution cryo-EM 3DR of OSU NTR and TR TLP. FSC resolution curves were calculated for OSU NTR-TLP (red, continuous line) and TR-TLP (red, dashed line). For the 0.5 threshold, values for OSU NTR- and TR-TLP were 16.2 and 17.4 Å, respectively; values for the 0.3 threshold were 14.3 and 15.4 Å, respectively.
Single-particle three-dimensional structures of OSU NTR- and TR-TLP. (A, D) Surface-shaded representation of the outer surfaces of NTR (A) and TR (D) particles, viewed along an icosahedral 2-fold axis. The surfaces are radially color-coded to represent VP4 or VP8*/VP5* spikes (red), VP7 (yellow) and VP6 (blue). The density is contoured at 1σ above the mean. The bar represents 100 Å. (B, E) Transverse sections, 2.8 Å thick, taken from the maps of NTR (B) and TR (E) TLP, parallel but displaced 34 Å from the central section, viewed along a 2-fold axis (darker, denser). Arrows indicate spikes in the surface-shaded representations in C and D and their corresponding densities in B and E. (C, F) Close up view of the NTR (C) and TR (F) spike represented as in A and D.
GenBank accession numbers for SA-C4111 and OSU-C5111 genomic segments.
EMDB accession codes for density maps.
We thank J.A. Melero for critical reading of the manuscript, M.C. Terrón, R. Marabini and J.J. Fernández for technical assistance and C. Mark for editorial assistance.
Conceived and designed the experiments: JMR DL. Performed the experiments: JMR FJC EMF FGC DL. Analyzed the data: JMR DL. Contributed reagents/materials/analysis tools: JLC JRC. Wrote the paper: JMR DL.
- 1. Marsh M, Helenius A (2006) Virus entry: open sesame. Cell 124: 729–740.
- 2. Estes MK, Greenberg HB (2013) Rotaviruses. In: Knipe DM, Howley PM, Cohen JI, Griffin DE, Lamb RA et al.., editors. Fields Virology. 6th ed. Philadelphia: Lippincott Williams & Wilkins.
- 3. Kotloff KL, Nataro JP, Blackwelder WC, Nasrin D, Farag TH, et al. (2013) Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study. Lancet 382: 209–222.
- 4. Parashar UD, Gibson CJ, Bresse JS, Glass RI (2006) Rotavirus and severe childhood diarrhea. Emerg Infect Dis 12: 304–306.
- 5. McClain B, Settembre E, Temple BR, Bellamy AR, Harrison SC (2010) X-ray crystal structure of the rotavirus inner capsid particle at 3.8 A resolution. J Mol Biol 397: 587–599.
- 6. Settembre EC, Chen JZ, Dormitzer PR, Grigorieff N, Harrison SC (2011) Atomic model of an infectious rotavirus particle. Embo J 30: 408–416.
- 7. Estrozi LF, Settembre EC, Goret G, McClain B, Zhang X, et al. (2013) Location of the dsRNA-dependent polymerase, VP1, in rotavirus particles. J Mol Biol 425: 124–132.
- 8. Periz J, Celma C, Jing B, Pinkney JN, Roy P, et al. (2013) Rotavirus mRNAS are released by transcript-specific channels in the double-layered viral capsid. Proc Natl Acad Sci U S A 110: 12042–12047.
- 9. Mathieu M, Petitpas I, Navaza J, Lepault J, Kohli E, et al. (2001) Atomic structure of the major capsid protein of rotavirus: implications for the architecture of the virion. Embo J 20: 1485–1497.
- 10. Ludert JE, Feng N, Yu JH, Broome RL, Hoshino Y, et al. (1996) Genetic mapping indicates that VP4 is the rotavirus cell attachment protein in vitro and in vivo. J Virol 70: 487–493.
- 11. Trask SD, Kim IS, Harrison SC, Dormitzer PR (2010) A rotavirus spike protein conformational intermediate binds lipid bilayers. J Virol 84: 1764–1770.
- 12. Chen JZ, Settembre EC, Aoki ST, Zhang X, Bellamy AR, et al. (2009) Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM. Proc Natl Acad Sci U S A 106: 10644–10648.
- 13. Clark SM, Roth JR, Clark ML, Barnett BB, Spendlove RS (1981) Trypsin enhancement of rotavirus infectivity: mechanism of enhancement. J Virol 39: 816–822.
- 14. Estes MK, Graham DY, Mason BB (1981) Proteolytic enhancement of rotavirus infectivity: molecular mechanisms. J Virol 39: 879–888.
- 15. Arias CF, Romero P, Alvarez V, Lopez S (1996) Trypsin activation pathway of rotavirus infectivity. J Virol 70: 5832–5839.
- 16. Gilbert JM, Greenberg HB (1998) Cleavage of rhesus rotavirus VP4 after arginine 247 is essential for rotavirus-like particle-induced fusion from without. J Virol 72: 5323–5327.
- 17. Dormitzer PR, Nason EB, Prasad BV, Harrison SC (2004) Structural rearrangements in the membrane penetration protein of a non-enveloped virus. Nature 430: 1053–1058.
- 18. Kim IS, Trask SD, Babyonyshev M, Dormitzer PR, Harrison SC (2010) Effect of mutations in VP5 hydrophobic loops on rotavirus cell entry. J Virol 84: 6200–6207.
- 19. Wolf M, Vo PT, Greenberg HB (2011) Rhesus rotavirus entry into a polarized epithelium is endocytosis dependent and involves sequential VP4 conformational changes. J Virol 85: 2492–2503.
- 20. Yoder JD, Trask SD, Vo TP, Binka M, Feng N, et al. (2009) VP5* rearranges when rotavirus uncoats. J Virol 83: 11372–11377.
- 21. Crawford SE, Mukherjee SK, Estes MK, Lawton JA, Shaw AL, et al. (2001) Trypsin cleavage stabilizes the rotavirus VP4 spike. J Virol 75: 6052–6061.
- 22. Chen DY, Ramig RF (1992) Determinants of rotavirus stability and density during CsCl purification. Virology 186: 228–237.
- 23. Prasad BV, Wang GJ, Clerx JP, Chiu W (1988) Three-dimensional structure of rotavirus. J Mol Biol 199: 269–275.
- 24. Bohl EH, Theil KW, Saif LJ (1984) Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. J Clin Microbiol 19: 105–111.
- 25. Bartesaghi A, Subramaniam S (2009) Membrane protein structure determination using cryo-electron tomography and 3D image averaging. Curr Opin Struct Biol 19: 402–407.
- 26. Fu CY, Johnson JE (2011) Viral life cycles captured in three-dimensions with electron microscopy tomography. Curr Opin Virol 1: 125–133.
- 27. Pesavento JB, Lawton JA, Estes ME, Venkataram Prasad BV (2001) The reversible condensation and expansion of the rotavirus genome. Proc Natl Acad Sci U S A 98: 1381–1386.
- 28. Scheres SH, Melero R, Valle M, Carazo JM (2009) Averaging of electron subtomograms and random conical tilt reconstructions through likelihood optimization. Structure 17: 1563–1572.
- 29. Dormitzer PR, Greenberg HB, Harrison SC (2001) Proteolysis of monomeric recombinant rotavirus VP4 yields an oligomeric VP5* core. J Virol 75: 7339–7350.
- 30. Kaljot KT, Shaw RD, Rubin DH, Greenberg HB (1988) Infectious rotavirus enters cells by direct cell membrane penetration, not by endocytosis. J Virol 62: 1136–1144.
- 31. Harrison SC (2008) Viral membrane fusion. Nat Struct Mol Biol 15: 690–698.
- 32. Mas V, Melero JA (2013) Entry of enveloped viruses into host cells: membrane fusion. Subcell Biochem 68: 467–487.
- 33. Chen J, Lee KH, Steinhauer DA, Stevens DJ, Skehel JJ, et al. (1998) Structure of the hemagglutinin precursor cleavage site, a determinant of influenza pathogenicity and the origin of the labile conformation. Cell 95: 409–417.
- 34. Wilson IA, Skehel JJ, Wiley DC (1981) Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution. Nature 289: 366–373.
- 35. Welch BD, Liu Y, Kors CA, Leser GP, Jardetzky TS, et al. (2012) Structure of the cleavage-activated prefusion form of the parainfluenza virus 5 fusion protein. Proc Natl Acad Sci U S A 109: 16672–16677.
- 36. Trask SD, Dormitzer PR (2006) Assembly of highly infectious rotavirus particles recoated with recombinant outer capsid proteins. J Virol 80: 11293–11304.
- 37. Burns JW, Chen D, Estes MK, Ramig RF (1989) Biological and immunological characterization of a simian rotavirus SA11 variant with an altered genome segment 4. Virology 169: 427–435.
- 38. Chen D, Burns JW, Estes MK, Ramig RF (1989) Phenotypes of rotavirus reassortants depend upon the recipient genetic background. Proc Natl Acad Sci U S A 86: 3743–3747.
- 39. Small C, Barro M, Brown TL, Patton JT (2007) Genome heterogeneity of SA11 rotavirus due to reassortment with "O" agent. Virology 359: 415–424.
- 40. Malherbe HH, Strickland-Cholmley M (1967) Simian virus SA11 and the related O agent. Arch Gesamte Virusforsch 22: 235–245.
- 41. Potgieter AC, Page NA, Liebenberg J, Wright IM, Landt O, et al. (2009) Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes. J Gen Virol 90: 1423–1432.
- 42. Lopez S, Arias CF (1992) Simian rotavirus SA11 strains. J Virol 66: 1832.
- 43. Mlera L, O'Neill HG, Jere KC, van Dijk AA (2013) Whole-genome consensus sequence analysis of a South African rotavirus SA11 sample reveals a mixed infection with two close derivatives of the SA11-H96 strain. Arch Virol 158: 1021–1030.
- 44. Patton JT, Chizhikov V, Taraporewala Z, Chen D (2000) Virus replication. Methods Mol Med 34: 33–66.
- 45. Arnold M, Patton JT, McDonald SM (2009) Culturing, storage, and quantification of rotaviruses. Curr Protoc Microbiol Chapter 15: Unit 15C 13.
- 46. Heymann JB, Belnap DM (2007) Bsoft: image processing and molecular modeling for electron microscopy. J Struct Biol 157: 3–18.
- 47. Scheres SH, Nunez-Ramirez R, Sorzano CO, Carazo JM, Marabini R (2008) Image processing for electron microscopy single-particle analysis using XMIPP. Nat Protoc 3: 977–990.
- 48. Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, et al. (2004) UCSF Chimera–a visualization system for exploratory research and analysis. J Comput Chem 25: 1605–1612.
- 49. Conway JF, Trus BL, Booy FP, Newcomb WW, Brown JC, et al. (1993) The effects of radiation damage on the structure of frozen hydrated HSV-1 capsids. J Struct Biol 111: 222–233.
- 50. Mindell JA, Grigorieff N (2003) Accurate determination of local defocus and specimen tilt in electron microscopy. J Struct Biol 142: 334–347.
- 51. Fernandez JJ, Luque D, Caston JR, Carrascosa JL (2008) Sharpening high resolution information in single particle electron cryomicroscopy. J Struct Biol 164: 170–175.
- 52. Kremer JR, Mastronarde DN, McIntosh JR (1996) Computer visualization of three-dimensional image data using IMOD. J Struct Biol 116: 71–76.
- 53. Fernandez JJ, Li S, Crowther RA (2006) CTF determination and correction in electron cryotomography. Ultramicroscopy 106: 587–596.
- 54. Agulleiro JI, Fernandez JJ (2011) Fast tomographic reconstruction on multicore computers. Bioinformatics 27: 582–583.