Citation: Wang Y (2013) Fungal Adenylyl Cyclase Acts As a Signal Sensor and Integrator and Plays a Central Role in Interaction with Bacteria. PLoS Pathog 9(10): e1003612. doi:10.1371/journal.ppat.1003612
Editor: William E. Goldman, The University of North Carolina at Chapel Hill, United States of America
Published: October 10, 2013
Copyright: © 2013 Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: Research by the author is funded by block funding to the Institute of Molecular and Cell Biology from the Agency for Science, Research and Technology, Singapore. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The author has declared that no competing interests exist.
Soon after birth, the human body establishes an intimate association with trillions of microbial cells belonging to numerous bacterial and fungal species . These organisms coinhabit diverse microbial communities on cutaneous and mucosal surfaces such as the skin, the gastrointestinal tract, and the vaginal cavity . Fungal and bacterial cells can interact in many ways, such as direct physical contact, secretion of toxins and signalling molecules, sharing or competing for metabolites and nutrients, and alteration of the environment . These interactions can be antagonistic as well as mutually beneficial. Both bacteria and fungi have evolved sophisticated mechanisms to sense and respond to the presence and activity of other species nearby. In hospitals, fungi and bacteria are frequently isolated from the same site of infection. This raises the important question of whether fungi and bacteria interact in the process of infection, and whether the interaction dictates disease development and outcome, and if so, how they do so. This review summarizes recent discoveries in the study of signal sensing in the fungal pathogen Candida albicans. New findings support a model that adenylyl cyclases act as a hub of signal sensing and integration and may play a central role in bacterial sensing during fungal infection.
cAMP Signalling Plays a Major Role in Regulating Cellular Responses to Environmental Signals and in Virulence in C. albicans
C. albicans is frequently found as a benign member of the normal microflora of humans. However, when conditions are favourable, it can cause a range of localized superficial infections such as rash and thrush in otherwise healthy people. But in immunocompromised patients, C. albicans can initiate life-threatening invasive infections with mortality rates as high as 75% . Several traits of this fungus determine its virulence, including its ability to switch growth forms between yeast, pseudohyphae, and hyphae, expression of surface adhesion proteins, and secretion of proteolytic enzymes. Importantly, these traits are coregulated primarily by the cAMP signalling pathway . A central component of this pathway is the cell's sole adenylyl cyclase Cyr1 that catalyses the synthesis of the second messenger 3′-5′-cyclic adenosine monophosphate (cAMP). In response to inducing signals, Cyr1 increases cAMP synthesis that in turn activates protein kinase A (PKA), leading to the expression of virulence genes. cyr1Δ/Δ mutants cannot undergo the yeast-to-hyphae transition and are avirulent . Many external signals of distinct nature such as peptidoglycan (PGN), CO2, pH, and temperature are known to stimulate Cyr1 activity. Then, how does Cyr1 distinguish different stimuli or sense and integrate multiple ones to initiate a proper physiological response?
Fungal Adenylyl Cyclases Are Large Proteins Containing Various Functional Domains Providing Multiple Points for Signal Sensing
Fungal Cyr1s contain several highly conserved domains (Figure 1), from the N- to C-terminus, including a Gα domain, a Ras-association (RA) domain, a leucine-rich repeat (LRR) domain, a protein phosphatase 2C (PP2C) domain, a cyclase catalytic (CYCc) domain, and a Cap1 (cyclase-associated protein 1) binding domain (CBD). In C. albicans, the small GTPase Ras1, when in the GTP-bound form, activates Cyr1 by binding to the RA domain . ras1Δ/Δ mutants are severely compromised in virulence and hyphal growth . Yeast-2-hybrid experiments demonstrated direct association of Ras1 with the RA domain, and mutating conserved residues in RA was shown to block Ras1-Cyr1 interaction and prevent adenylyl cyclase activation . Ras1 is thought to be activated by the guanine nucleotide exchange factor Cdc25 and inactivated by the GTPase-activating protein Ira2 . Currently, it remains unclear as to what regulates the Ras regulatory module. The Gα domain of Cyr1 is thought to be the binding site for a G-protein α subunit Gpa2 that is activated by the G-protein-coupled receptor Gpr1 in response to amino acids and glucose , . Deleting either GPR1 or GPA2 caused defects in hyphal formation on solid media in a cAMP-dependent manner. Although Gpa2 has been shown to bind to the Gα domain in fission yeast , such interaction has not been demonstrated in C. albicans. Cap1 is a well-known Cyr1-associated and G-actin-binding protein and is required for the activation of fungal adenylyl cyclase. C. albicans cap1Δ/Δ mutants are unable to increase cAMP synthesis upon hyphal induction, fail to undergo the yeast-to-hyphae transition, and are avirulent . Recently, Zou et al.  isolated a tripartite protein complex containing Cap1, Cyr1, and G-actin, in which Cap1 serves as a bridge by binding to Cyr1 and G-actin through its N- and C-terminus, respectively. This complex can enhance cAMP synthesis in response to hyphal-induction signals in vitro, and in a manner dependent on Cap1 interaction with G-actin. This study suggests that Cyr1 may be able to sense the status of the actin cytoskeleton, a central player in polarized growth, and influence the cyclase activity. The Cap1 binding site has been mapped to the C-terminal tail of Cyr1 . Raising temperature to above 37°C is normally required for the yeast-to-hyphae transition. The mechanism of temperature sensing was found to involve the heat shock protein complex Hsp90/Sgt1 through physical association with Cyr1 , . In S. cerevisiae, Sgt1 was shown to influence cAMP signalling via direct interaction with the LRR domain of Cyr1 . High CO2 concentration, found in many host niches, is another promoter of hyphal growth. Klengel et al.  discovered that CO2/bicarbonate activates Cyr1 by targeting the catalytic domain. In summary, four distinct domains of Cyr1 serve as sensor for signals as diverse as sugar, gas, temperature, and actin.
Evidence from many studies of the past decade or so supports a model in which fungal adenylyl cyclases function as a hub of signal sensing and integration. This figure illustrates all the conserved domains in fungal adenylyl cyclases and the external and internal signals each of the domains senses in C. albicans adenylyl cyclase Cyr1. For abbreviations and protein names, please refer to the text.
C. albicans Cyr1 Directly Senses Bacterial PGN
Serum at 37°C is probably the strongest and physiologically relevant inducer of hyphal growth in C. albicans. Although the serum activity was first reported in 1956, the identity of the active molecule(s) remained as a mystery for decades. Recently, Xu et al.  discovered PGN fragments in serum fractions with high hyphal-inducing activity, and later confirmed that several muramyl dipeptides (MDPs), subunits of peptidoglycan, were indeed potent hyphal inducers. Mass spectrometry analysis detected ∼0.1 to 0.5 µM MDP in the blood of all healthy volunteers. As PGN is uniquely present in bacterial cell wall, the human microbiota is most likely the provider of PGN in the blood. Many bacteria are known to release a large amount of bioactive PGN subunits into the environment during cell wall remodelling . Xu et al.  also demonstrated that MDP activates Cyr1 by binding to the LRR domain. Various mutations in the LRR domain completely abolished the hyphal growth induced by serum and MDP. This discovery has significant implications for the possible role of bacteria in C. albicans infection. As a commensal, C. albicans is constantly exposed to PGN fragments continuously released by trillions of bacterial cells. Although its effect on adenylyl cyclase activation may be balanced by other antagonistic factors, certain conditions may tip the balance in favour of C. albicans infection. For example, the use of broad-spectrum antibiotics, most of which inhibit PGN synthesis, may cause a massive release of PGN fragments. Together with antibiotic-associated colitis that damages the intestinal epithelium, PGN may enter the blood stream in large quantities, creating a window of opportunity for C. albicans to initiate systemic infection. This could be an important yet unappreciated factor underlying the high risk of candidemia in patients receiving high doses of broad-spectrum antibiotics.
Farnesol and Bacterial Quorum-Sensing Molecules Inhibit C. albicans Hyphal Growth by Targeting the Catalytic Domain of Adenylyl Cyclase
Farnesol is a quorum-sensing molecule (QSM) produced by C. albicans that inhibits hyphal development and biofilm formation . Early studies provided evidence suggesting that farnesol exerts its effect by interfering with the Ras/cAMP/PKA pathway , . Hall et al.  later discovered that farnesol directly inhibits the cyclase activity of a truncated version of Cyr1 embracing the catalytic domain alone. Interestingly, the bacterial QSM 3-oxo-C12-homoserine lactone (HSL) secreted by Paseudomonas aeruginosa also inhibits C. albicans hyphal growth by a similar mechanism . This mode of intertaxon chemical communication has important implications in the cause of microbial infections and ways to treat them. In the human microbiota, bacteria account for >99% of all microbial cells, which effectively checks fungal growth through secreting QSMs among other antagonistic mechanisms. However, disturbance of a microbial community by an antibacterial therapy may release the “brake” and create opportunities for commensal fungi such as C. albicans to initiate infection.
Currently, the evidence is strong for fungal adenylyl cyclases as a coincidence detector . To understand how their activity is kept low in the absence of stimuli and is turned on by different ligands either individually or in combination, structural elucidation of fungal adenylyl cyclases is urgently needed particularly in complex with interacting proteins and ligands. Also, the role of the LRR domain in signal sensing deserves more attention. A long LRR domain is present in most pattern recognition receptors of the innate immune system in animals and plants that recognizes a wide range of microbe-associated molecular patterns to elicit immune response . So far, little can be found in the literature on the role of the LRR domain in fungal adenylyl cyclases. In C. albicans Cyr1, the LRR domain senses PGN. It is important to know whether the LRR domain in other fungal adenylyl cylases also plays a role in bacterial sensing. As members of the class III adenylyl cyclases, dimerization is required for catalysis . However, it remains entirely unknown whether there is a dynamic and regulated monomer-dimer interconversion in fungal adenylyl cyclases. Equally elusive is their cellular localization. Answers to these questions may unveil additional dimensions for regulation.
- 1. Human Microbiome Project Consortium (2012) Structure, function and diversity of the healthy human microbiome. Nature 486: 207–214.
- 2. Findley K, Oh J, Yang J, Conlan S, Deming C, et al. (2013) Topographic diversity of fungal and bacterial communities in human skin. Nature 498: 367–370. doi: 10.1038/nature12171
- 3. Peleg AY, Hogan DA, Mylonakis E (2010) Medically important bacterial-fungal interactions. Nat Rev Microbiol 8: 340–349. doi: 10.1038/nrmicro2313
- 4. Brown GD, Denning DW, Gow NAR, Levitz SM, Netea MG, et al. (2012) Hidden killers; human fungal infections. Sci Transl Med 4: 165rv13. doi: 10.1126/scitranslmed.3004404
- 5. Kumamoto CA, Vinces MD (2005) Contributions of hyphae and hypha-co-regulated genes to Candida albicans virulence. Cell Microbiol 7: 1546–1554. doi: 10.1111/j.1462-5822.2005.00616.x
- 6. Rocha CR, Schröppel K, Harcus D, Marcil A, Dignard D, et al. (2001) Signaling through adenylyl cyclase is essential for hyphal growth and virulence in the pathogenic fungus Candida albicans. Mol Biol Cell 12: 3631–3643. doi: 10.1091/mbc.12.11.3631
- 7. Fang HM, Wang Y (2006) RA domain-mediated interaction of Cdc35 with Ras1 is essential for increasing cellular cAMP level for Candida albicans hyphal development. Mol Microbiol 61: 484–496. doi: 10.1111/j.1365-2958.2006.05248.x
- 8. Feng Q, Summers E, Guo B, Fink G (1999) Ras signaling is required for serum-induced hyphal differentiation in Candida albicans. J Bacteriol 181: 6339–6346.
- 9. Hogan DA, Sundstrom P (2009) The Ras/cAMP/PKA signaling pathway and virulence in Candida albicans. Future Microbiol 4: 1263–1270. doi: 10.2217/fmb.09.106
- 10. Maidan MM, Thevelein JM, Van Dijck P (2005) Carbon source induced yeast-to-hypha transition in Candida albicans is dependent on the presence of amino acids and on the G-protein-coupled receptor Gpr1. Biochem Soc Trans 33: 291–293. doi: 10.1042/bst0330291
- 11. Maidan MM, De Rop L, Serneels J, Exler S, Rupp S, et al. (2005) The G protein-coupled receptor Gpr1 and the Galpha protein Gpa2 act through the cAMP-protein kinase A pathway to induce morphogenesis in Candida albicans. Mol Biol Cell 16: 1971–1986. doi: 10.1091/mbc.e04-09-0780
- 12. Ivey FD, Hoffman CS (2005) Direct activation of fission yeast adenylate cyclase by the Gpa2 Galpha of the glucose signaling pathway. Proc Natl Acad Sci U S A 102: 6108–6113. doi: 10.1073/pnas.0502270102
- 13. Bahn YS, Sundstrom P (2001) CAP1, an adenylate cyclase-associated protein gene, regulates bud-hypha transitions, filamentous growth, and cyclic AMP levels and is required for virulence of Candida albicans. J Bacteriol 183: 3211–3223. doi: 10.1128/jb.183.10.3211-3223.2001
- 14. Zou H, Fang HM, Zhu Y, Wang Y (2009) A sensor/effector apparatus for activating cAMP synthesis in Candida albicans hyphal growth. Mol Microbiol 75: 579–591. doi: 10.1111/j.1365-2958.2009.06980.x
- 15. Bai C, Xu XL, Wang HS, Wang YM, Chan FY, et al. (2011) Characterization of a hyperactive Cyr1 mutant reveals new regulatory mechanisms for cellular cAMP levels in Candida albicans. Mol Microbiol 82: 879–893. doi: 10.1111/j.1365-2958.2011.07859.x
- 16. Shapiro RS, Uppuluri P, Zaas AK, Collins C, Senn H, et al. (2009) Hsp90 orchestrates temperature-dependent Candida albicans morphogenesis via Ras1-PKA signalling. Curr Biol 19: 621–629. doi: 10.1016/j.cub.2009.03.017
- 17. Shapiro RS, Zaas AK, Betancourt-Quiroz M, Perfect JR, Cowen LE (2012) The Hsp90 co-chaperone Sgt1 governs Candida albicans morphogenesis and drug resistance. PLoS ONE 7: e44734 doi:10.1371/journal.pone.0044734.
- 18. Dubacq C, Guerois R, Courbeyrette R, Kitagawa K, Mann C (2002) Sgt1p contributes to cyclic AMP pathway activity and physically interacts with the adenylyl cyclase Cyr1p/Cdc35p in budding yeast. Eukaryot Cell 1: 568–582. doi: 10.1128/ec.1.4.568-582.2002
- 19. Klengel T, Liang WJ, Chaloupka J, Ruoff C, Schröppel K, et al. (2005) Fungal adenylyl cyclase integrates CO2 sensing with cAMP signaling and virulence. Curr Biol 15: 2021–2026. doi: 10.1016/j.cub.2005.10.040
- 20. Xu XL, Lee RTH, Fang HM, Wang YM, Li R, et al. (2008) Bacterial peptidoglycan triggers Candida albicans hyphal growth by directly activating the adenylyl cyclase Cyr1p. Cell Host Microbe 4: 28–39. doi: 10.1016/j.chom.2008.05.014
- 21. Cloud-Hansen KA, Peterson SB, Stabb EV, Goldman WE, McFall-Ngai , et al. (2006) Breaching the great wall: peptidoglycan and microbial interactions. Nat Rev Microbiol 4: 710–716. doi: 10.1038/nrmicro1486
- 22. Langford ML, Atkin AL, Nickerson KW (2009) Cellular interactions of farnesol, a quorum-sensing molecule produced by Candida albicans. Future Microbiol 4: 1353–1362. doi: 10.2217/fmb.09.98
- 23. Davis-Hanna A, Piispanen AE, Stateva LI, Hogan DA (2008) Farnesol and dodecanol effects on the Candida albicans Ras1-cAMP signalling pathway and the regulation of morphogenesis. Mol Microbiol 67: 47–62. doi: 10.1111/j.1365-2958.2007.06013.x
- 24. Lindsay AK, Deveau A, Piispanen AE, Hogan DA (2012) Farnesol and cyclic AMP signaling effects on the hypha-to-yeast transition in Candida albicans. Eukaryot Cell 11: 1219–1225. doi: 10.1128/ec.00144-12
- 25. Hall RA, Turner KJ, Chaloupka J, Cottier F, De Sordi L, et al. (2011) The quorum-sensing molecules farnesol/homoserine lactone and dodecanol operate via distinct modes of action in Candida albicans. Eukaryot Cell 10: 1034–1042. doi: 10.1128/ec.05060-11
- 26. Hogan DA, Muhlschlegel FA (2011) Candida albicans developmental regulation: adenylyl cyclase as a coincidence detector of parallel signals. Curr Opin Microbiol 14: 682–686. doi: 10.1016/j.mib.2011.09.014
- 27. Soanes DM, Talbot NJ (2010) Comparative genome analysis reveals an absence of leucine-rich repeat pattern recognition receptor proteins in the kingdom fungi. PLoS ONE 5: e12725 doi:10.1371/journal.pone.0012725.
- 28. Linder JU (2006) Class III adenylyl cyclases: molecular mechanisms of catalysis and regulation. Cell Mol Life Sci 63: 1736–1751. doi: 10.1007/s00018-006-6072-0