20 Jun 2014:
The PLOS Pathogens Staff
Analysis of the transcriptome of Borrelia burgdorferi, the causative agent of Lyme disease, during infection has proven difficult due to the low spirochete loads in the mammalian tissues. To overcome this challenge, we have developed an In Vivo Expression Technology (IVET) system for identification of B. burgdorferi genes expressed during an active murine infection. Spirochetes lacking linear plasmid (lp) 25 are non-infectious yet highly transformable. Mouse infection can be restored to these spirochetes by expression of the essential lp25-encoded pncA gene alone. Therefore, this IVET-based approach selects for in vivo-expressed promoters that drive expression of pncA resulting in the recovery of infectious spirochetes lacking lp25 following a three week infection in mice. Screening of approximately 15,000 clones in mice identified 289 unique in vivo-expressed DNA fragments from across all 22 replicons of the B. burgdorferi B31 genome. The in vivo-expressed candidate genes putatively encode proteins in various functional categories including antigenicity, metabolism, motility, nutrient transport and unknown functions. Candidate gene bbk46 on essential virulence plasmid lp36 was found to be highly induced in vivo and to be RpoS-independent. Immunocompetent mice inoculated with spirochetes lacking bbk46 seroconverted but no spirochetes were recovered from mouse tissues three weeks post inoculation. However, the bbk46 gene was not required for B. burgdorferi infection of immunodeficient mice. Therefore, through an initial IVET screen in B. burgdorferi we have identified a novel in vivo-induced virulence factor critical for the ability of the spirochete to evade the humoral immune response and persistently infect mice.
Lyme disease is caused by tick-bite transmission of the pathogenic spirochete Borrelia burgdorferi. An increased understanding of how B. burgdorferi survives throughout its infectious cycle is critical for the development of innovative diagnostic and therapeutic protocols to reduce the incidence of Lyme disease. One of the major difficulties blocking this effort has been genome-wide identification of the B. burgdorferi genes that are expressed in the mammalian host environment. Using in vivo expression technology (IVET) in B. burgdorferi for the first time, we have identified B. burgdorferi genes that are expressed during an active murine infection. We demonstrate that candidate gene bbk46, encoded on essential linear plasmid 36, is highly expressed in vivo and, unlike some other known B. burgdorferi in vivo-induced genes, is not RpoS regulated. Spirochetes lacking bbk46 establish an infection in mice and elicit an antibody response but are undetectable in mouse tissues three weeks post inoculation. The bbk46 is not required for spirochete infection of mice lacking a functional immune system. In sum, development of an IVET-based approach in B. burgdorferi has identified a novel virulence gene critical for the spirochete's ability to evade the mammalian adaptive immune response.
Citation: Ellis TC, Jain S, Linowski AK, Rike K, Bestor A, Rosa PA, et al. (2013) In Vivo Expression Technology Identifies a Novel Virulence Factor Critical for Borrelia burgdorferi Persistence in Mice. PLoS Pathog 9(8): e1003567. doi:10.1371/journal.ppat.1003567
Editor: Jenifer Coburn, Medical College of Wisconsin, United States of America
Received: February 27, 2013; Accepted: July 1, 2013; Published: August 29, 2013
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Funding: Research reported in this publication was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award numbers K22AI081730 (MWJ) and R01AI099094 (MWJ), the Intramural Research Program, NIAID/NIH (PAR), the National Research Fund for Tick-Borne Diseases 2012–2013 pilot grant (MWJ) and UCF startup funds (MWJ). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Lyme disease is a multi-stage inflammatory disease caused by the pathogenic spirochete Borrelia burgdorferi, which is transmitted by the bite of an infected tick . B. burgdorferi has an enzootic life cycle that requires persistence in two disparate environments, the arthropod vector and the mammalian host. B. burgdorferi is well adapted to modulate its expression profile in response to the different conditions encountered throughout its infectious cycle . Although the specific environmental signals that induce changes in spirochete gene expression are not fully defined, it has been reported that changes in temperature, pH, the presence or absence of mammalian blood, as well as changes in bacterial growth rate, can affect patterns of gene expression –. DNA microarray analysis and proteomics have been used to examine changes in the global expression profile of B. burgdorferi grown under in vitro conditions that partially mimic the tick and mouse environments –. A rat dialysis membrane chamber (DMC) implant model, together with microarray technology, has been used to help identify B. burgdorferi genes expressed in response to mammalian host-specific signals –. Although the data reported in these studies provide insight into the molecular mechanisms of gene regulation, they may not fully reflect the patterns of B. burgdorferi gene expression during an active mammalian infection. Furthermore, transcriptome analysis of B. burgdorferi during murine infection has proven difficult given that spirochete loads in the blood and tissues are too low to recover sufficient spirochete RNA for direct microarray analysis .
In vivo expression technology (IVET) is a gene discovery method used to identify transcriptionally active portions of a microbial genome during interaction of the microorganism with a particular environment or host organism , . In this system, the environment itself directly selects for upregulated bacterial loci . The IVET selection system functions on the premise that deletion of a biosynthetic gene can lead to attenuation of growth and persistence of a pathogen in the host environment. This attenuation can be complemented by expression of the biosynthetic gene driven by promoters that are transcriptionally active in vivo. Thus, in the environment of interest, in vivo transcriptionally active promoters can be selected from a genomic library of DNA fragments cloned upstream of the essential biosynthetic gene –. IVET is a sensitive and versatile method for identification of in vivo-expressed genes that has been used with pathogenic bacteria and fungi in a wide variety of host environments and has identified a number of previously uncharacterized virulence genes –.
Using IVET we have developed and applied a genome-wide genetic screening approach to identify B. burgdorferi genes that are expressed during an active murine infection. This is the first time that an IVET strategy has been applied to B. burgdorferi. Moreover, we have identified a novel gene on virulence plasmid lp36 that is strongly induced in vivo and required for B. burgdorferi persistent infection in the mouse.
Mammalian host-adapted spirochetes demonstrate a 100-fold decrease in ID50 relative to in vitro grown spirochetes
It is clear that B. burgdorferi modulates its gene expression profile at different stages of the infectious cycle . The infectious dose of wild-type B. burgdorferi varies depending on the environment from which the spirochetes are derived. For example, the 50% infectious dose (ID50) of spirochetes derived from partially fed ticks has been found to be two orders of magnitude lower than that of log phase in vitro grown B. burgdorferi . In order to quantitatively assess the impact that adaptation to the mammalian environment has on B. burgdorferi infectivity the 50% infectious dose (ID50) of spirochetes derived directly from the mammalian host was determined and compared to that of log phase in vitro grown spirochetes. B. burgdorferi are only transiently present in the blood of immunocompetent mice , whereas spirochetes persist longer in the blood of immunocompromised mice . Therefore, the blood of severe combined immunodeficiency (scid) mice infected with B. burgdorferi was used as a source of spirochetes adapted to the mammalian environment. Strikingly, an inoculum containing approximately eight in vivo-derived spirochetes was able to infect five out of six mice, whereas, 5,000 in vitro grown spirochetes were required to obtain this level of infectivity (Table 1). The ID50 for in vivo-derived spirochetes was found to be less than eight organisms. In contrast, the ID50 for in vitro grown spirochetes was calculated to be 660 organisms. These data indicate that mammalian host-adapted spirochetes are 100-fold more infectious than in vitro grown spirochetes, likely due to appropriate coordinate expression of in vivo-expressed genes important for murine infectivity.
The B. burgdorferi IVET system is a robust method for selection of B. burgdorferi sequences that are expressed during murine infection
We have developed a genome-wide genetic screening method to identify B. burgdorferi genes that are expressed during mouse infection using an in vivo expression technology (IVET) approach , . The in vivo expression technology vector, pBbIVET, carries the B. burgdorferi bmpB Rho-independent transcription terminator sequence  repeated in triplicate (3XTT), to prevent any read-through promoter activity from the pBSV2* Borrelia shuttle vector backbone, followed by the promoter-less in vivo-essential pncA gene (Figure 1) , . Spirochetes lacking linear plasmid (lp) 25 are non-infectious in mice and severely compromised in the tick vector –. The pncA gene, located on lp25, encodes a nicotinamidase that is sufficient to restore murine infectivity to B. burgdorferi lacking the entire lp25 plasmid . Genetic transformation of low-passage, infectious B. burgdorferi occurs at low frequency and efficiency hampering introduction of a complex DNA library into an infectious background , . Because B. burgdorferi clones lacking lp25 and lp56 demonstrate increased transformability , we isolated a clonal derivative of the low-passage infectious clone A3 that lacks both lp25 and lp56. This clone was designated A3 68-1 . Clones A3 and A3 68-1 were transformed by electroporation with 20 µg of a Borrelia shuttle vector. The transformation frequency and efficiency of A3 68-1 was determined relative to that of the A3 parent. As expected, genetic transformation of A3 68-1 occurred at a high frequency and efficiency. We recovered approximately 2,000 transformants/ml in clone A3 68-1, whereas no transformants were recovered with a parallel transformation of clone A3 (data not shown). In order to test the function of the B. burgdorferi IVET system, the promoter for the in vivo essential ospC gene , was cloned in front of the promoter-less pncA gene in pBbIVET (Figure 1), creating plasmid pBbIVET-ospCp. This plasmid, along with pBbIVET alone, was transformed into the non-infectious, low-passage, highly transformable B. burgdorferi clone A3 68-1. All clones were tested for their abilities to infect groups of 6 C3H/HeN mice at an infectious dose 100 (ID100) of 1×104 spirochetes , indicating the presence or absence of an active promoter sufficient to drive expression of pncA thereby restoring infectivity. Spirochetes were reisolated from the ear, bladder and joint tissues of 5/6 mice infected with B. burgdorferi harboring pBbIVET-ospCp. No spirochetes were reisolated from mice (0/6) infected with B. burgdorferi carrying the promoter-less pBbIVET alone. Together these data demonstrated that our promoter trap system functioned with a known in vivo active promoter.
Features of this vector include: 3XTT, the transcriptional terminator sequence for bmpB  repeated in triplicate; pncA, promoterless pncA gene; flgBpkan, kanamycin resistance cassette; zeo, zeocin resistance marker; ColE1, E. coli origin of replication; ORFs 1, 2, 3, B. burgdorferi cp9 replication machinery. The EcoRI restriction site was used to clone the B. burgdorferi control in vivo-expressed promoter, ospCp, as well as the B. burgdorferi (Bb) gDNA library, in front of the promoterless pncA gene. The pBbIVET vector was derived from the B. burgdorferi shuttle vector pBSV2* .
Screening for B. burgdorferi genes expressed during murine infection
A B. burgdorferi genomic DNA library using an average DNA fragment size of approximately 200 bps was constructed upstream of the promoter-less pncA gene (Figure 1) in the pBbIVET vector in E. coli, yielding approximately 30,000 independent clones. A small subset of individuals from the 30,000 clone library in E. coli was analyzed by PCR and DNA sequencing and determined to carry non-identical B. burgdorferi DNA fragments. The strategy used to construct the pBbIVET library allowed the DNA fragments to be cloned in either the forward or reverse orientation relative to the pncA gene. Therefore, a library of 30,000 clones each harboring a unique 200 bp DNA fragment represented approximately 2× coverage of the 1.5 Mb genome of B. burgdorferi. Although the initial analysis of the transformation efficiency of B. burgdorferi clone A3 68-1 demonstrated that each transformation of 20 µg of a single purified plasmid into this genetic background yielded approximately 10,000 transformants, this transformation efficiency was not achieved when 20 µg of complex library plasmid DNA was transformed into A3 68-1. Forty four transformations of the library plasmid DNA resulted in recovery of approximately 15,000 individual clones in B. burgdorferi A3 68-1, representing an IVET library in B. burgdorferi with approximately 1× coverage of the spirochete genome. As described for the pBbIVET library in E. coli, a subset of individuals from 15,000 clone library in B. burgdorferi were analyzed and found to carry non-identical B. burgdorferi DNA fragments.
Like the BbIVET system described here, many IVET strategies are based upon complementation of auxotrophy. For microorganisms other than B. burgdorferi these strategies have allowed negative selection against “promoter-less” clones in minimal medium in which the auxotroph mutants are unable to grow . B. burgdorferi lacking pncA are not attenuated for growth in the complex, undefined B. burgdorferi medium, BSKII. Moreover, there is currently no minimal medium available that supports the growth of wild-type B. burgdorferi. Therefore, the BbIVET system did not include negative selection against “promoter-less” clones in vitro. 179 mice were infected with pools from the B. burgdorferi IVET library of approximately 100 clones each, with each clone at a dose of 1×104 spirochetes resulting in a total dose of 1×106 spirochetes per mouse. Three weeks post inoculation, mice were sacrificed and ear, heart, bladder and joint tissues were harvested for reisolation of infectious spirochetes. 175 out of 179 mice became infected with B. burgdorferi as determined by reisolation of spirochetes from at least two or more of the tissue sites analyzed (Table 2). However, due to the potentially stochastic nature of the kinetics of infection  and/or tissue-specific promoter activity of distinct B. burgdorferi genomic fragments not all four tissue sites from all 175 infected mice were found to be positive for spirochete reisolation. Nonetheless, the recovery of live spirochetes from infected mouse tissues suggested that these spirochetes harbored in vivo active promoter(s) in the pBbIVET plasmid sufficient to drive expression of the in vivo-essential pncA gene to restore spirochete mouse infectivity. Total genomic DNA was isolated from each pool of reisolated spirochetes from each of the four mouse tissues and the pBbIVET plasmid DNA rescued in E. coli. Colony PCR using primers targeting the genomic DNA insert region of the pBbIVET vector was performed on 24 of the resulting E. coli colonies from each plasmid rescue transformation. No reisolated spirochetes were found to harbor a pBbIVET plasmid lacking a genomic DNA fragment insert. The amplified inserts were analyzed by restriction digest using a cocktail of the A/T-rich restriction enzymes to identify those DNA fragments with distinct restriction patterns, suggesting that these fragments represent different in vivo active promoters. Up to eleven non-identical restriction digest patterns were detected for every subset of 24 E. coli transformants carrying pBbIVET DNA that were analyzed (Figure S1). The DNA fragments corresponding to each distinct restriction digest pattern were further analyzed by DNA sequencing and the identities of the sequences determined by microbial genome BLAST analysis. Screening of approximately 15,000 BbIVET clones through mice resulted in the identification of 289 non-identical B. burgdorferi in vivo-expressed (Bbive) DNA fragments from across the chromosome and all 21 plasmid replicons of the B. burgdorferi B31 segmented genome (Table 2). Although the 1∶1 molar ratio of insert to vector used to generate the pBbIVET library did not preclude insertion of more than one fragment into each clone, only 20 out of the 289 clones were found to harbor two distinct DNA fragments. Of these clones the 3′ DNA fragment, proximal to the pncA ORF, was assumed to be the active promoter and was included in the subsequent analyses.
B. burgdorferi in vivo expressed promoters map to distinct classes of putative regulatory sequences across the genome
Genomic mapping of the 289 unique Bbive promoters identified in this genetic screen demonstrated that 67% of the sequences mapped to sense DNA in the same direction as annotated open reading frames, 27% mapped to antisense DNA in the opposite direction to annotated open reading frames and 6% mapped to intergenic regions lacking annotated open reading frames. Of the large percentage of sense sequences, 41%, which represented 28% of the total Bbive sequences, mapped to regions just upstream of and in the same orientation to annotated open reading frames, suggesting that these sequences are promoters for the associated open reading frames and that these open reading frames are candidate in vivo-expressed genes. The remaining 59% of the sense sequences, which represented 39% of the total Bbive sequences, mapped within annotated open reading frames, suggesting the possibility for promoter elements within B. burgdorferi genes. Similar findings of putative transcriptional start sites within genes and operons have been reported for other bacterial pathogens , . The sequences that mapped to putative promoter locations in the genome and the genes associated with these promoters were prioritized for further analysis. Among the list of 80 sequences, 9 promoter regions were represented by two overlapping genomic DNA fragments. Five of these overlapping sequence pairs shared the same 3′ end, suggesting that the sequences belonging to each pair contained the same promoter. Whereas, the other four overlapping sequence pairs harbored distinct 3′ ends, suggesting that each sequence contained a unique promoter. The 71 in vivo-expressed candidate genes have been annotated to encode proteins in various functional categories including: cell division, cell envelope, replication, metabolism, motility, protein synthesis, transport and unknown functions (Table 3).
IVET identified candidate gene bbk46 on virulence plasmid lp36
Linear plasmid 36 is required for B. burgdorferi mouse infection; however, the genetic elements on lp36 that contribute to this phenotype have not been fully defined . IVET identified a candidate in vivo-expressed promoter sequence, Bbive162, which mapped to lp36. This sequence was found to be 60 bp long, with 48 bp immediately upstream of and in the same direction as the BBK46 open reading frame (Figure 2), suggesting that the bbk46 gene may be expressed during mammalian infection and may contribute to the essential role of lp36 in B. burgdorferi infectivity. Therefore, the bbk46 gene was selected for further analysis.
The reverse complement of nucleotides 28391 to 29474 on lp36, encompassing bbk46 (Genbank GeneID: 1194234) and its putative promoter sequence. The nucleotide sequence of Bbive162 is underlined. The putative ribosome binding site is shown in bold italics. The putative BBK46 amino acid sequence is shown in bold. The stop codons at nucleotides 625 and 820 are highlighted in gray. The position of the inserted FLAG-epitope tag sequence is indicted with a star (*). The position of the inserted cMyc-epitope tag sequence is indicated with a number sign (#).
Expression of the bbk46 gene is induced during murine infection
Our BbIVET screen identified gene bbk46 as a putative in vivo-expressed gene. The BbIVET screen was designed to identify B. burgdorferi DNA fragments that are expressed in vivo and did not discriminate between those promoters that are specifically induced in vivo and those promoters that are expressed both in vitro and in vivo. Therefore, quantitative reverse transcription PCR (qRT-PCR) was used to validate the expression of bbk46 in vivo and to determine whether bbk46 expression was upregulated in vivo compared to in vitro. Total RNA was isolated from bladder tissue collected from mice infected with 1×105 wild-type B. burgdorferi three weeks post-inoculation as well as log phase in vitro grown spirochetes. RNA was converted to cDNA using random hexamer primers and the mRNA level of each target gene was measured relative to the constitutive recA gene using quantitative PCR. The gene expression levels of flaB and ospC were also measured as control constitutively-expressed and in vivo-induced genes, respectively. These data demonstrated that although bbk46 was expressed during in vitro growth, expression of this gene was increased more than 100-fold during mammalian infection (Figure 3A). Consistent with their known patterns of gene regulation, flaB expression was relatively unchanged in vivo compared to in vitro; whereas, ospC demonstrated a nearly 1000-fold increase in expression in vivo compared to in vitro (Figure 3A). Moreover, the relative amount of bbk46 expression during in vitro growth was found to be approximately 10-fold more than that of ospC. Whereas, the in vivo expression levels of genes bbk46, ospC and flaB were similar.
Total RNA was isolated from bladder tissue collected from (A) mice infected with 1×105 wild-type B. burgdorferi three weeks post-inoculation (in vivo, gray bars) and from log phase in vitro grown spirochetes (in vitro, white bars) or (B) stationary phase temperature-shifted stationary phase in vitro grown wild-type (white bars) or ΔrpoS (gray bars) B. burgdorferi. RNA was reverse transcribed to cDNA using random hexamer primers. The expression of bbk46, flaB and ospC were quantified using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and a standard curve analysis method. The mRNA levels of the bbk46, flaB and ospC gene transcripts were normalized to that of the constitutive recA gene. The data are expressed as the gene transcript/recA transcript. The data represent the average of triplicate qRT-PCR analyses of 3 biological replicates. Error bars represent the standard deviation from the mean.
RpoS is a global regulator that controls expression of genes expressed during mammalian infection, including ospC . Because bbk46 expression was induced in vivo in a manner similar to that of ospC, we sought to determine whether, like ospC, bbk46 is an RpoS-regulated gene. RNA was isolated from stationary phase temperature-shifted wild-type and ΔrpoS mutant spirochetes, a growth condition previously shown to induce expression of rpoS and rpoS-regulated genes . Quantitative RT-PCR was then performed for genes bbk46, flaB, ospC and recA, as described above. As expected, ospC expression was increased approximately 20 times in the presence compared to the absence of rpoS (Figure 3B). In contrast, bbk46 expression was RpoS-independent under these growth conditions (Figure 3B). Likewise, no RpoS-dependent change in gene expression was detected for flaB. Interestingly, the amount of flaB expression detected in the stationary phase temperature-shifted spirochetes (Figure 3B) was dramatically decreased compared to the amount of flaB expression detected in log phase and in vivo grown spirochetes (Figure 3A), suggesting that flaB is not expressed at the same level under all growth conditions. Together these data demonstrated that bbk46 was highly induced during murine infection and bbk46 expression was not controlled by RpoS during in vitro growth.
The bbk46 open reading frame fails to produce detectable amounts of protein during in vitro growth
The bbk46 gene is a member of paralogous gene family 75, which also includes lp36-encoded genes bbk45, bbk48 and bbk50, all of which are annotated to encode putative P37 immunogenic lipoproteins – (Figure 4). These genes are located on the right arm of lp36 in B. burgdorferi clone B31, which is a highly variable region among distinct B. burgdorferi isolates . The members of paralogous gene family 75 are conserved within B. burgdorferi isolates but are not present in the relapsing fever spirochetes. The B. burgdorferi clone B31 BBK45, BBK48 and BBK50 proteins are predicted to be 301, 288 and 332 amino acids, respectively. However, B. burgdorferi clone B31 bbk46 is annotated as a pseudogene as a result of an authentic frame shift resulting in a TAA stop codon at nucleotide 625 –, thereby producing a putative 209 amino acid protein. In contrast, the BBK46 homolog in clone N40, BD04, harbors a CAA codon at nucleotide 625, resulting in a glutamic acid residue at amino acid 209 and producing a putative 273 amino acid protein . Sequence analysis of the cloned bbk46 open reading frame confirmed the presence of the TAA stop codon at nucleotide 625 (Figure 2). To experimentally determine the size of the BBK46 protein produced in B. burgdorferi B31 the bbk46 ORF along with a FLAG epitope tag sequence prior to the stop codon at nucleotide 625 and a cMyc epitope tag sequence prior to the stop codon at nucleotide 820 (Figure 2) was cloned into the B. burgdorferi shuttle vector pBSV2G under the control of either the constitutive flaB promoter or the putative endogenous bbk46 promoter. A mutant clone lacking the entire BBK46 open reading frame was constructed by allelic exchange and verified by PCR analysis (Figure 5A and 5B). The bbk46 mutant clone was transformed with the shuttle vectors carrying the epitope tagged bbk46 constructs. All transformants were verified to contain the plasmid content of the parent clone. BBK46 protein production was assessed in both E. coli and B. burgdorferi. Immunoblot analyses using αFLAG and αcMyc monoclonal antibodies resulted in detection of a FLAG-epitope tagged protein of an approximate molecular mass of 23 kDa, which is the predicted size of the 209 amino acid BBK46 protein, in the E. coli clones carrying both the flaBp-driven and the bbk46p-driven constructs (Figure 6). Surprisingly, no FLAG epitope tagged protein was detected in either B. burgdorferi clone (Figure 6), although bbk46 gene expression was observed in these clones (data not shown), indicating that the lack of detectable BBK46 protein was not likely the result of a transcription defect. Furthermore, no cMyc epitope tagged protein was detected in either E. coli or B. burgdorferi. Together these data suggested that although the bbk46 ORF is competent to produce a 23 kDa protein in E. coli and the transcript is expressed in B. burgdorferi during in vitro growth, the protein is either not produced or is rapidly turned over in log phase in vitro grown B. burgdorferi.
Shown is an amino acid alignment of the P37 protein family members BBK45 (GenBank accession no. NP_045617.2), BBK46 (translated bbk46, Genbank GeneID: 1194234), BBK48 (GenBank accession no. NP_045619.1) and BBK50 (GenBank accession no. NP_045621.1). Amino acids identical to the consensus sequence are shaded. The predicted SpLip lipobox sequence  is indicted with five stars. Dashes represent spaces introduced for optimal sequence alignment. The positions of the two stop codons in the bbk46 translation are indicated with arrows. Amino acid sequences were aligned using the CLUSTAL W algorithm in the MEGALIGN program from the DNASTAR Lasergene suite.
(A) Schematic representation of the wild-type (WT) and Δbbk46 loci on lp36. The sequence of the entire bbk46 open reading frame was replaced with a flaBp-aadA antibiotic resistance cassette , . Locations of primers for analysis of the mutant clones are indicated with small arrows and labels P7–P12, P19 and P20. Primer sequences are listed in Table 5. (B) PCR analysis of the Δbbk46 mutant clone. Genomic DNA isolated from WT and Δbbk46/vector spirochetes served as the template DNA for PCR analyses. DNA templates are indicated across the bottom of the gel image. The primer pairs used to amplify specific DNA sequences are indicated at the top of the gel image and correspond to target sequences as shown in A. Migration of the DNA ladder in base pairs is shown to the left of each image. (C) In vitro growth analysis of mutant clones. A3-68ΔBBE02 (WT), bbk46::flaBp-aadA/pBSV2G (Δbbk46/vector) and bbk46::flaBp-aadA/pBSV2G-bbk46 (Δbbk46/bbk46+) spirochetes were inoculated in triplicate at a density of 1×105 spirochetes/ml in 5 ml of BSKII medium. Spirochete densities were determined every 24 hours under dark field microscopy using a Petroff-Hausser chamber over the course of 96 hours. The data are represented as the number of spirochetes per ml over time (hours) and is expressed as the average of 3 biological replicates. Error bars indicate the standard deviation from the mean.
Immunoblot analysis of total protein lysate prepared from 1.5×108 B. burgdorferi Δbbk46 (Bb) or E. coli harboring either pBSV2G flaBp-bbk46-FLAG-cMyc (flaBp) or pBSV2G bbk46p-bbk46-FLAG-cMyc (bbk46p). Protein lysates were separated by SDS-PAGE and immunoblots performed using anti-FLAG monoclonal antibodies (α FLAG) and anti-cMyc monoclonal antibodies (α cMyc). 300 ng of purified PncA-FLAG  and GST-BmpA-cMyc  proteins served as positive controls (+) for each antibody. The positions of markers to the left of the panel depict protein standard molecular masses in kilodaltons.
As a putative member of the P37 immunogenic lipoprotein family, BBK46 is predicted to localize to the spirochete outer surface and to be immunogenic during mammalian infection. Therefore, recombinant BBK46, lacking the first 32 amino acids that are predicted to comprise the signal sequence for the lipoprotein, was produced in E. coli as an N-terminal fusion to glutathione S-transferase (GST). To assess the immunogenicity of the BBK46 protein, immunoblot analysis was performed using purified rGST-BBK46 probed with mouse immune serum collected 21 days post inoculation with 1×104 wild-type B. burgdorferi. The rGST-BBK46 protein was found to be non-immunoreactive with mouse immune serum, in contrast to the control antigen BmpA (Figure S2). These data suggest that, if produced in B. burgdorferi, BBK46 is not an immunoreactive antigen. However, these data do not rule out the possibility that the immunogenic epitope is not present or available in the recombinant protein produced in E. coli.
The bbk46 gene is required for B. burgdorferi persistence in immunocompetent mice
In vitro growth analysis demonstrated that the bbk46 mutant and complemented clones had no detectable in vitro phenotypes (Figure 5C). Therefore, to examine the role of bbk46 in mouse infectivity, groups of five C3H/HeN female mice were needle inoculated intradermally under the skin of the upper back with 1×104 wild-type, Δbbk46/vector or Δbbk46/bbk46+ spirochetes. Three weeks post inoculation, mice were assessed for B. burgdorferi infection by serology and reisolation of spirochetes from the inoculation site, ear, bladder and joint tissues. All five mice from each infection group were seropositive for anti-B. burgdorferi antibodies (Figure 7, Table 4). Surprisingly however, no spirochetes were reisolated from all tissues examined from the five mice inoculated with the Δbbk46/vector clone (Table 4), whereas, all five mice inoculated with the wild-type or the Δbbk46/bbk46+ clone resulted in reisolation of spirochetes from all tissues analyzed (Table 4). Together these data demonstrated that spirochetes lacking the bbk46 gene transiently infected and elicited a humoral response in mice, but were unable to maintain a persistent infection in mouse tissues. To further define the contribution of the host immune response to the inability of spirochetes lacking the bbk46 genes to cause a persistent infection, groups of five severe combined immunodeficiency (scid) mice were inoculated with 1×104 wild-type, Δbbk46/vector or Δbbk46/bbk46+ spirochetes. Three weeks post inoculation the animals were assessed for infection by reisolation of spirochetes from the ear, bladder and joint tissues. Consistent with the hypothesized role of bbk46 in immune evasion and persistence, four out of five immunodeficient mice inoculated with the Δbbk46 mutant were positive for spirochete reisolation from all tissues examined (Table 4). These data demonstrated that a functional host immune response is required for the clearance of spirochetes lacking bbk46 from mouse tissues 3 weeks post infection, indicating that bbk46 is essential for the ability of B. burgdorferi to avoid killing by the host immune system in order to establish a persistent infection.
Immunoblot analysis of sera collected three weeks post inoculation from groups of five C3H/HeN mice inoculated with clone A3-68ΔBBE02 (WT), bbk46::flaBp-aadA/pBSV2G (Δbbk46/vector) and bbk46::flaBp-aadA/pBSV2G-bbk46 (Δbbk46/bbk46+) at a dose of 1×104 spirochetes per mouse. (A) Total protein lysate from B. burgdorferi clone B31 A3 was probed with the serum from each individual mouse (1–5). (B) Purified recombinant GST-OspC protein was probed with pooled sera from the five mice in each infection group or αOspC polyclonal antibodies. The positions of markers to the left of the panel depict protein standard molecular masses in kilodaltons.
In this study we have successfully adapted and applied for the first time an IVET-based genetic screen for use in B. burgdorferi for the purpose of identifying spirochete genes that are expressed during mammalian infection. Historically, genetic manipulation of low passage, infectious B. burgdorferi has been challenged by the low transformation frequencies of these spirochetes, preventing application of classic in vivo genetic screening techniques such as in vivo expression technology (IVET) and signature-tagged mutagenesis (STM)  to identify B. burgdorferi genetic elements important for pathogenicity. However, advances in the understanding of the B. burgdorferi restriction modification systems that inhibit transformation , – have recently allowed construction and characterization of a comprehensive STM mutant library in infectious B. burgdorferi . The foundation for our strategy for development of IVET in B. burgdorferi was based upon the spirochete's requirement of lp25 for both restriction modification and virulence functions. Spirochetes lacking lp25 are highly transformable but non-infectious in mice –, . Restoration of the lp25-encoded pncA gene to lp25− spirochetes restores wild-type infectivity  but maintains high transformation frequency. At the time of the development of the pBbIVET system the true start codon of the pncA gene was not defined; therefore, the promoter-less pncA gene construct in the pBbIVET plasmid used an engineered AUG start codon and was missing the first 24 nucleotides of the now defined pncA ORF . Furthermore, this construct was purposefully designed without a ribosome binding site (RBS) and was dependent upon the cloned B. burgdorferi DNA fragments to contain both a promoter and a functional RBS. Although we acknowledge that this requirement may have limited the number of clones identified in our screen, during development of the BbIVET system we found that inclusion of an RBS sequence in the promoterless pncA construct resulted in vector-driven PncA production in the absence of a promoter. Thus, in order to reduce the possibility of recovering false positive clones, the pBbIVET system was designed without an RBS. The enzyme Tsp509I was selected to generate the DNA fragments for the pBbIVET library because the AATT restriction site of this enzyme is present approximately every 58 bp in the B. burgdorferi B31 genome. However, it is possible that DNA fragments generated with this enzyme will not result in sequences that contain a 3′ RBS appropriately distanced from the start codon of the pncA ORF, thereby limiting the number of clones identified in the screen.
Screening of a 15,000 clone B. burgdorferi genomic library in mice identified 289 DNA sequences from across all 22 B. burgdorferi replicons capable of promoting pncA expression resulting in an infectious phenotype. It is likely that the BbIVET screen did not achieve saturation because the number of clones analyzed was only estimated to cover the B. burgdorferi genome one time, under the assumption that each cloned DNA fragment in the library was unique. Analysis of the pBbIVET library in B. burgdorferi suggested that the library was composed of 15,000 unique clones. However, because only a small fraction of the library was examined for the sequences of the DNA fragment inserts, our findings do not rule out the potential that the library was composed of less than 15,000 non-identical clones and therefore, may represent less than 1× coverage of the genome. Of the 175 mice infected with the pBbIVET library, 10% resulted in reisolation of a single clone, 62% resulted in reisolation of two to five unique clones, and 28% resulted in reisolation of six to eleven unique clones. Furthermore, 57% of the 289 Bbive sequences were only recovered once; whereas, 39% of the sequences were recovered two to five times and 4% of the sequences were recovered six to twelve times. These data are indicative of the amount of redundancy in the screen and suggest that although the screen may not have been representative of the entire B. burgdorferi genome, a large percentage of mice became infected with multiple clones and many of the Bbive sequences were recovered more than once.
We found that 71 of the Bbive sequences mapped to canonical promoter positions upstream of annotated open reading frames in the B. burgdorferi genome. Unexpectedly, the well characterized in vivo-expressed ospC promoter was not among these sequences. However, the ospCp was successfully recovered in our functional validation of the BbIVET system, suggesting that the BbIVET screen had not reached complete saturation of the genome and with further screening of the BbIVET library the ospCp sequence may be recovered. Alternatively, given that ospC expression is known to be down-regulated after the initial stages of infection , – it is possible that in the context of a mixed infection individual pBbIVET clones carrying the ospCp lack a fitness advantage due to decreased expression three weeks post inoculation and may not be recovered in our screen. This explanation may appear to conflict with the findings reported herein that ospC expression is high at three weeks post inoculation and the ospCp served as a robust positive control promoter for the BbIVET system. However, down-regulation of ospC expression at this time point in infection is a stochastic process that occurs at the level of the individual spirochete and does not occur simultaneously across the entire population . Although at the population level the ospCp is expressed at this time point in our studies, in the context of the BbIVET screen individual clones carrying the ospCp may express reduced amount of pncA and may be out competed by other BbIVET clones carrying stronger promoters.
A subset of the genes identified in the BbIVET screen included known in vivo-expressed genes, which provided validation that our genetic system was working as expected and was sufficiently powerful. The screen recovered the promoter for genes bba36 (Bbive175), bba57 (Bbive271), bbb27 (Bbive240), bbj34 (Bbive267), bbj36 (Bbive171), bbj51 (Bbive269), bb0213 (Bbive15) and bb0760 (Bbive94), all of which have been shown previously to be expressed during mammalian infection . Furthermore, bba57 was recently reported to be up-regulated in vivo and to contribute to pathogenesis in the mouse . The bptA gene encodes a function that has been shown to be required for B. burgdorferi survival in the tick and to contribute to mouse infectivity , . In addition, Bbive14, 58, 232, 84, 269, 295 and 77 are associated with genes that have been shown to be up-regulated in in vivo-like conditions and/or gene products that are immunogenic in humans and mice , , , . Notably, few in vivo-expressed candidate genes identified using BbIVET were previously observed to be up-regulated in mammalian host-adapted spirochetes derived from growth within rat dialysis membrane chambers (DMCs). Genes identified in our analyses that have also been detected by microarray analysis of DMC grown spirochetes include bba36 , , bbj51 , , bb0551, bbm28 , bb0495, and bb0660 . The results of the DMC microarray studies are reported as genes that are significantly up-regulated in DMC-derived spirochetes relative to spirochetes grown in vitro; whereas, the BbIVET screen does not distinguish between genes that are specifically induced in vivo and genes that are expressed both in vitro and in vivo. Furthermore, the environmental cues within the DMCs may not fully reflect those experienced by B. burgdorferi during an active infection. Finally, the BbIVET system specifically selects for promoters that are capable of driving expression of pncA allowing the spirochetes to survive throughout a three week mouse infection. Together, these technical and biological differences between the DMC microarray and BbIVET screen likely contributed to the distinct results obtained from the two methods of gene expression analysis. In addition, few genes that have been previously established to be RpoS-regulated in vitro and/or within DMCs ,  were identified by the BbIVET screen. RpoS-regulated genes bba36, bba57, bb0265 and bbh01 ,  were among the in vivo-expressed Bbive candidate genes. Similarly, only one putative BosR-regulated gene, bb0592 , was identified in the BbIVET screen. Although it is unclear why only a small number of know RpoS-regulated promoters were recovered, the recently identified AT-rich BosR binding site  contains the restriction site for the Tsp509I restriction enzyme used to generate the BbIVET library. Therefore, it is possible that the BosR binding sites were subject to cleavage by Tsp509I, perhaps resulting in a limited number of DNA fragments that contained BosR-dependent promoters.
The BbIVET screen was carried out in such a way that both DNA fragments that are expressed in vitro and in vivo, as well as those fragments that are specifically induced in vivo, could be recovered. Therefore, it was not surprising that genes encoding cell division, DNA replication, energy metabolism, protein synthesis and transcription functions were identified, all of which are likely functions essential for spirochete growth under all condition. These findings were consistent with those categories of genes not recovered by genome-wide transposon mutagenesis, suggesting that these genes encode essential functions . The BbIVET screen identified genes that encode proteins in functional categories that may contribute to B. burgdorferi infectivity and pathogenesis including, putative lipoproteins, motility and chemotaxis proteins, transport proteins and proteins of unknown function. Similarly, transposon mutagenesis analysis indicated that motility and chemotaxis genes as well as transport genes are important for B. burgdorferi survival in the mouse .
Linear plasmid 36 is known to be critical for B. burgdorferi survival in the mouse; however, the genes on lp36 that contribute to this requirement have not been fully characterized . The recently published comprehensive STM study suggests that many of the genes encoded on lp36 participate in B. burgdorferi infectivity , . BbIVET identified gene bbk46 on lp36. We found that bbk46 was expressed both in vitro and in vivo. However, bbk46 expression was dramatically induced in spirochetes isolated from infected mouse tissues as compared to spirochetes grown in vitro, suggesting a possible role for this gene in B. burgdorferi infectivity. Moreover, consistent with lack of identification of bbk46 as an RpoS-regulated genes in previous studies of the RpoS regulon , ,control of bbk46 expression was found to be RpoS-independent under in vitro growth conditions that typically induce expression of rpoS regulated genes , , , . These findings highlight the power and uniqueness of the IVET-based approach for identification of B. burgdorferi in vivo-expressed genes, which might not be discovered using other genome-wide gene expression methods. Surprisingly, BBK46 protein was not detected in spirochetes expressing FLAG epitope tagged bbk46 under the control of the putative native promoter or the constitutive flaB promoter. Moreover, sera from B. burgdorferi infected mice were non-immunoreactive against recombinant BBK46 protein. In support of these data, no peptide corresponding to BBK46 has been detected in genome-wide proteome analysis of B. burgdorferi under different environmental conditions . Our findings suggest that despite high gene expression, the encoded BBK46 protein is produced at low levels in the spirochete and/or BBK46 is rapidly turned over in the cell. Alternatively, bbk46 may function as an RNA. The molecular nature of the functional product of bbk46 is currently under investigation.
Deletion of bbk46 from low-passage, infectious B. burgdorferi resulted in no observable in vitro growth defect. Immunocompetent mice needle inoculated with spirochetes lacking bbk46 were found to be seropositive for B. burgdorferi antibodies three weeks post-infection, although the serological responses appeared to be slightly diminished relative to those of mice infected with the wild-type and complemented clones. Surprisingly, however, no live spirochetes were reisolated from all tissues examined from the mutant infected mice at this same time point. Conversely, all mice infected with the wild-type or complemented clone were both seropositive and reisolation positive. Furthermore, bbk46 was not required for spirochete survival in immunocompromised mice. These data indicate that bbk46 is dispensable for the initial stages of B. burgdorferi murine infection but this gene is essential for B. burgdorferi persistence in mouse tissues and may contribute to a mechanism of spirochete evasion of host-acquired immune defenses.
B. burgdorferi survival in the mammalian host requires diverse mechanisms that allow the spirochete to resist and evade the host's immune responses. However, the genetic components of these important properties of the pathogen have yet to be well defined. Here we demonstrate that spirochetes lacking bbk46 establish an initial infection and are seroreactive but are unable to persist in murine tissues following host antibody production. To our knowledge a similar phenotype has been documented for only two other B. burgdorferi genes, the lp28-1 encoded vls antigenic variation locus , , – and the chromosomally encoded lmp-1(bb0210) gene . Moreover, analogous to the bbk46 mutant, the phenotypes of spirochetes lacking a functional vls locus as well as spirochetes lacking lmp-1 have been shown to be dependent on the host immune response as these mutants demonstrate wild-type survival under immune privileged growth conditions and in immunocompromised mice , , –. Although it is clear that the antigenic switching mechanism conferred by the vls locus is essential for B. burgdorferi persistence in the host , , the precise mechanism of vls-dependent immune evasion remains unknown. Similarly the mechanism of lmp-1-dependent protection of B. burgdorferi against the host's humoral immune response is unknown . VlsE and Lmp-1 are highly antigenic proteins present on the outer surface of the spirochete –. The BBK46 open reading frame appears to encode a lipoprotein with a predicted signal sequence for outer surface localization; however, recombinant BBK46 protein produced in E. coli was not found to be seroreactive when analyzed by immunoblot using immune sera collected from mice infected with wild-type B. burgdorferi. Future studies are focused on elucidation of the role of bbk46 in the pathogenesis of B. burgdorferi.
In conclusion, we have developed and applied the IVET technology to B. burgdorferi to identify spirochete genes expressed during mammalian infection. This represents the first use of this system in B. burgdorferi. The power of this system was validated by identification of a subset of genes that have been demonstrated previously to be upregulated in vivo. Furthermore, IVET identified bbk46, a novel, uncharacterized gene located on essential virulence plasmid lp36. We have presented evidence that bbk46 is highly upregulated during B. burgdorferi murine infection and is critical for the spirochete's ability to persistently infect immunocompetent mouse tissues. Further analysis of the molecular mechanism of bbk46-promoted survival, as well as identification and characterization of other putative virulence factors identified by BbIVET, will contribute to advancing understanding of in vivo persistence and pathogenicity of B. burgdorferi.
Materials and Methods
The University of Central Florida is accredited by the International Association for Assessment and Accreditation of Laboratory Animal Care. Protocols for all animal experiments were prepared according to the guidelines of the National Institutes of Health and were reviewed and approved by the University of Central Florida Institutional Animal Care and Use Committee (Protocol numbers 09-38 and 12-42).
Bacteria clones and growth conditions
All B. burgdorferi clones used were derived from clone B31 A3. Clone A3 68-1, which lacks lp25 and lp56  was used for the pBbIVET library. The B31 A3 wild-type and rpoS::kan B. burgdorferi clones  were used for gene expression experiments. All low-passage B. burgdorferi mutant and complemented clones generated herein were derived from infectious clone A3-68ΔBBE02, which lacks cp9, lp56 and gene bbe02 on lp25 . B. burgdorferi was grown in liquid Barbour-Stoenner-Kelly (BSK) II medium supplemented with gelatin and 6% rabbit serum  and plated in solid BSK medium as previously described , . All spirochete cultures were grown at 35°C supplemented with 2.5% CO2. Kanamycin was used at 200 µg/ml, streptomycin was used at 50 µg/ml and gentamicin was used at 40 µg/ml, when appropriate. All cloning steps were carried out using DH5α E. coli, which were grown in LB broth or on LB agar plates containing 50 µg/ml kanamycin, 300 µg/ml spectinomycin or 10 µg/ml gentamicin.
Generation of the pBbIVET plasmid
The promoterless pncA gene was amplified from B. burgdorferi B31 genomic DNA using primers 1 and 2 (Table 5) and Taq DNA polymerase (New England Biolabs). The EcoRI/XbaI-digested pncA fragment was cloned into EcoRI/XbaI-linearized plasmid pBSV2*TT , creating plasmid pBbIVET. The in vivo-expressed ospC promoter with EcoRI ends was amplified from B. burgdorferi B31 genomic DNA using primers 3 and 4 (Table 5) and cloned into the EcoRI-cut, Antarctic phosphatase-treated (New England Biolabs) pBbIVET plasmid in front of the promoterless pncA gene, resulting in plasmid pBbIVET ospCp. All plasmids were analyzed and verified by restriction digest and sequence analysis. The pBbIVET and pBbIVET ospCp plasmids were each transformed by electroporation into A3 68-1  as described  and transformants selected in solid BSK medium containing kanamycin and confirmed by PCR using primers 1 and 2 (Table 5). Total genomic DNA was prepared from PCR-positive clones and screened for the presence of the B. burgdorferi plasmid content . The clones that retained the plasmid content of the parent clone were used in further experiments.
Generation of the BbIVET library
Total genomic DNA was isolated from a 250 ml culture of B. burgdorferi B31 clone A3 grown to a density 1×108 spirochetes/ml using the Qiagen genomic DNA buffer set and Genomic-tip 500/G, according to the manufacturer's protocol (Qiagen). A3 genomic DNA was partially digested with Tsp509I (New England Biolabs). The partial digests were electrophoretically separated on a 0.8% agarose gel and the 300 to 500 bp range of DNA fragments extracted and ligated in a 1∶1 molar ratio with EcoRI-digested and Antarctic phosphatase-treated pBbIVET. Library ligations were electroporated into E. coli Top10 cells (Life Technologies) and transformants selected on LB agar containing 50 µg/ml kanamycin, resulting in approximately 30,000 independent clones. Plasmid DNA was isolated from these cells and 20 µg aliquots of the plasmid library were transformed by electroporation into B. burgdorferi A3 68-1, as previously described . One fifth of each transformation was plated on solid BSK medium containing kanamycin. B. burgdorferi pBbIVET colonies were verified to contain B. burgdorferi DNA fragments by PCR using primers 5 and 6 (Table 5) and the number of transformants recovered quantitated. The approximately 15,000 B. burgdorferi clones recovered over 40 transformations were stored in aliquots of pools of approximately 100 BbIVET clones each in 25% glycerol at −80°C.
Selection of B. burgdorferi clones having in vivo-expressed DNA fragments
Each BbIVET pool (∼100 clones) was grown in 10 ml of fresh BSKII medium to a density of 1×108 spirochetes/ml. In groups of approximately 20 animals, 144 6–8 week old C3H/HeN female mice were each inoculated (80% intraperitoneal and 20% subcutaneous) with a dose 1×106 spirochetes of a unique pool of ∼100 BbIVET clones, under the assumption that each clone was present at dose 1×104 spirochetes. A fraction of each inoculum was plated on solid BSK medium and colonies screened for the presence of virulence plasmid lp28-1. Three weeks post inoculation, spirochetes were reisolated from ear, heart, bladder and joint tissues in 10 ml BSKII medium containing 20 µg/ml phosphomycin (Sigma), 50 µg/ml rifampicin (Sigma) and 2.5 mg/ml amphotericin B (Sigma) in 0.2% dimethyl sulfoxide (Sigma). Total genomic DNA was isolated from each spirochete cultures using the Wizard genomic DNA purification kit (Promega) and transformed into chemically competent E. coli DH5α cells and colonies selected on LB agar containing kanamycin to recover the pBbIVET plasmids. Twenty four transformants were chosen at random from each plasmid rescue and colony PCR performed using primers 5 and 6 (Table 5) to amplify the in vivo-expressed DNA fragment. PCR products were subsequently digested with a cocktail of restriction enzymes (DraI, SspI and AseI) and visualized on a 1% agarose gel. Approximately 14,000 E. coli clones were analyzed in this manner. All unique BbIVET fragments, as determined by the restriction digest pattern (Figure S1), were analyzed by direct sequencing of the PCR product using primer 5. Each individual sequence was identified by blastn analysis and mapped to its location in the B. burgdorferi B31 genome.
Deletion of bbk46
We used a PCR-based overlap extension strategy to delete the bbk46 gene. A spectinomycin/streptomycin resistance cassette, flaBp-aadA  with blunt ends, was amplified from genomic DNA isolated from clone ΔguaAB  using Phusion High-fidelity DNA polymerase (Thermo Scientific) and primers 11 and 12 (Table 5). The 500 bp flanking region upstream of the bbk46 ORF was amplified from the B. burgdorferi B31 clone A3 genomic DNA using the Phusion High-fidelity DNA polymerase and primers 7 and 8 (Table 5). This introduced a 25 bp sequence at the 3′ end of this fragment that was complementary to the 5′ end of the flaBp-aadA cassette. Similarly, the 500 bp flanking region downstream of the bbk46 ORF was amplified using the primers 9 and 10 (Table 5), which introduced a 5′ sequence of 30 bp that was complementary to the 3′ end of the resistance cassette. The PCR products from the above 3 reactions were mixed in equal volumes and used as a template for a fourth amplification reaction using Phusion High-fidelity DNA polymerase and primers 7 and 10 (Table 5) in order to generate a product containing the resistance cassette flanked by the 500 bp sequences upstream and downstream of the bbk46 ORF. This product was ligated with linear pCR-Blunt using a Zero Blunt PCR cloning Kit (Life technologies), yielding the allelic exchange plasmid pCR-Blunt-Δbbk46-flaBp-aadA. B. burgdorferi A3-68ΔBBE02 was transformed with 20 µg of pCR-Blunt-Δbbk46-flaBp-aadA purified from E. coli as previously described . Streptomycin-resistant colonies were confirmed to be true transformants by PCR using primer pairs 7 and 10 and 11 and 12 (Table 5). Positive Δbbk46-flaBp-aadA clones were screened with a panel of primers  for the presence of all of the B. burgdorferi plasmids of the parent A3-68ΔBBE02 clone , and a single clone was selected for further experiments.
Complementation of the Δbbk46 mutant
A PCR-based overlap extension strategy was used to create a DNA fragment encompassing the bbk46 gene and putative upstream promoter sequence with the introduction of a FLAG epitope tag immediately upstream of the putative premature stop codon and a cMyc epitope tag immediately upstream of the downstream stop codon. This was done by using Phusion High-fidelity DNA polymerase (New England Biolabs) and the primers pairs 13 and 14, 15 and 16, and 17 and 18 (Table 5). A KpnI restriction site was introduced at the 5′ end of this fragment and a SalI site at the 3′ end. The KpnI+SalI-digested PCR product was ligated into KpnI+ SalI-digested B. burgdorferi shuttle vector pBSV2G  and cloned in E. coli. The pBSV2G bbk46p-bbk46-FLAG-cMyc plasmid structure and sequence were confirmed by restriction digest and DNA sequence analysis. In addition, a 400 bp DNA fragment encompassing the flaB promoter with KpnI and BamHI ends was amplified from B31 A3 genomic DNA using primers 27 and 28 (Table 5). The KpnI+BamHI-digested PCR product was ligated into KpnI+ BamHI-digested B. burgdorferi shuttle vector pBSV2G . The bbk46-FLAG-cMyc gene without the putative bk46 promoter sequence and with BamHI and SalI ends was amplified from pBSV2G bbk46p-bbk46-FLAG-cMyc plasmid DNA using Phusion High-fidelity DNA polymerase (New England Biolabs) and primers 29 and 18 (Table 5). The BamHI+SalI-digested PCR product was ligated into BamHI+SalI-digested pBSV2GflaBp and cloned in E. coli. The pBSV2G flaBp-bbk46-FLAG-cMyc plasmid structure and sequence were confirmed by restriction digest and DNA sequence analysis. The Δbbk46 mutant was transformed with 20 µg of pBSV2G bbk46p-bbk46-FLAG-cMyc, pBSV2G flaBp-bbk46-FLAG-cMyc or pBSV2G alone isolated from E. coli and positive transformants selected as previously described , . The clones that retained the B. burgdorferi plasmid content of the parent clone were selected for use in further experiments.
Immunoblot analysis of BBK46-FLAG-cMyc
Production of the BBK46-FLAG-cMyc protein was examined in both E. coli and B. burgdorferi carrying pBSV2G bbk46p-bbk46-FLAG-cMyc or pBSV2G flaBp-bbk46-FLAG-cMyc. Total E. coli protein lysates were prepared from 2×109 cells harvested following overnight growth in LB medium at 37°C with aeration. E. coli cells were resuspended and lysed in 200 µl B-PER protein extraction reagent (Pierce), followed by the addition of 200 µl 2× Laemmli sample buffer plus 2-mercaptoethanol (Bio-rad). Total B. burgdorferi protein lysates were prepared from 2×109 spirochetes harvested at mid-log phase. The spirochetes were washed twice in 1 ml cold HN buffer (50 mM Hepes, 50 mM NaCl, pH 7.4) and lysed in 200 µl B-PER protein extraction reagent (Thermo Scientific), followed by the addition of 200 µl 2× Laemmli sample buffer plus 2-mercaptoethanol (Bio-rad). 30 ml of each protein lysate (∼1.5×108 cells) were separated by SDS-PAGE and transferred to a nitrocellulose membrane. 300 ng of PncA-FLAG  and GST-BmpA-cMyc  proteins served as positive controls. Immunoblot analysis was performed using anti-FLAG monoclonal primary antibody (Genscript) diluted 1∶500 in Tris-buffered saline, pH 7.4 and 0.5% Tween20 (TBST) and goat anti-mouse IgG+IgM-HRP secondary antibody (EMD Millipore) diluted 1∶10,000 in TBST and the signal detected using SuperSignal West Pico chemluminescent substrate kit (Thermo Scientific). The membrane was then stripped using 0.2 M NaOH, reblocked using 5% skim milk in TBST and probed with anti-cMyc primary antibody (Genscript) diluted 1∶500 in TBST and goat anti-mouse IgG+IgM-HRP (EMD Millipore) and visualized as described above.
Cloning, purification and seroreactivity analysis of rGST-BBK46
An in-frame glutathinone S-transferase (GST)-BBK46 fusion protein lacking the putative BBK46 signal sequence was generated using primers 30 and 31 (Table 5) and purified, as previously described . Approximately 1 µg of GST-BBK46 was separated by SDS-PAGE, transferred to a nitrocellulose membrane and analyzed by immunoblot for seroreactivity using immune serum collected 3 weeks post inoculation from mice infected with wild-type B. burgdorferi as previously described . Controls included 1 µg of GST alone and total protein lysates generated from BL21 E. coli, B. burgdorferi B31 A3 and E. coli expressing B. burgdorferi bmpA  prepared as described above. The membrane was stripped as described above and reprobed with anti-GST primary monoclonal antibody (EMD Millipore) diluted 1∶1000 in TBST and goat anti-mouse IgG+IgM-HRP (EMD Millipore) and visualized as described above.
In vitro growth analysis
Wild-type (A3-68ΔBBE02), Δbbk46/vector and Δbbk46/bbk46+ spirochetes were inoculated in triplicate at a density of 1×105 spirochetes/ml in 5 ml of BSK II medium. Spirochete densities were determined every 24 hours under dark field microscopy using a Petroff-Hausser chamber over the course of 96 hours.
RNA isolation from in vitro grown spirochetes
To obtain in vitro grown log phase spirochetes, wild-type (B31 A3) spirochetes were grown in triplicate in 5 ml of BSKII medium pH 7.5 at 35°C to a density of 3×107 spirochetes/ml. To obtain stationary phase, temperature-shifted spirochetes, wild-type (B31 A3) spirochetes were grown in triplicate in 5 ml of BSKII medium pH 7.5 at 35°C to a density of 3×107 spirochetes/ml, transferred to 25°C for 48 hours and then returned to 35°C for an additional 24–36 hours to a density of 2×108 spirochetes/ml. A total of 1×107 spirochetes were harvested from each culture and total RNA was isolated using TRIzol reagent (Life Technologies) according to the manufacturer's instructions. RNA was resuspended in 100 µl DEPC-treated dH2O. RNA was treated with TURBO DNA-free (Life Technologies) to remove any contaminating genomic DNA. 1 µl of Riboguard (40 U/µl) RNAse inhibitor (Epicentre) was added to all samples and RNA stored at −80°C.
RNA isolation from infected mouse tissue
B. burgdorferi-infected mouse bladders (see mouse infection experiments below) were manually macerated on ice using sterile scalpels and transferred to a 2 ml sterile tube containing lysing Matrix D (MP Biomedicals). 1 ml of RNA pro solution (FastRNA Pro Green kit, MP Biomedicals) was added to each sample on ice. Tissues were homogenized using a PowerGen High-Throughput Homogenizer (Fisher Scientific) following six cycles of beating for 45 sec and 2 minute incubations on ice. Samples were centrifuged at 13,000 rpm for 5 minutes at 4°C. The upper aqueous phase was transferred to new tubes and incubated for 5 minutes at room temperature. 500 µl of 1-bromo-3-chloropropane (Sigma Aldrich) and 45 µl of 5 M sodium acetate were added to each sample and samples were incubated for an additional 5 minutes at room temperature. Samples were centrifuged at 13,000 rpm for 5 minutes at 4°C. The upper aqueous phase was transferred to new tubes and RNA precipitated with the addition of 500 µl of absolute ethanol and 1 µl GlycoBlue (Life technologies). RNA was pelleted by centrifugation at 13,000 rpm for 10 minutes at 4°C. RNA was washed with 70% ethanol in DEPC-treated dH20 and resuspended in 100 µl DEPC-treated dH20. RNA was treated with TURBO DNA-free (Life Technologies) to remove any contaminating genomic DNA. 1 µl Riboguard (40 U/µl) RNAse inhibitor (Epicentre) was added to all samples and RNA stored at −80°C.
Gene expression analysis
cDNA was synthesized from 1.0 µg of each RNA sample using the iScript cDNA synthesis kit (Bio-Rad) with random primers according to the manufacturer's instructions. Parallel cDNA reactions were carried out in the absence of reverse transcriptase. Real-time quantitative PCR (qPCR) reactions were prepared using 1 µg of each cDNA and iQ SYBR Green Supermix (Bio-Rad). Using an Applied Biosystems 7500 instrument, samples were assayed for the flaB, recA, ospC and bbk46 transcripts using primers pairs 21 and 22, 23 and 24, 25 and 26, and 19 and 20, respectively (Table 5). Standard curves were generated for each gene target using 100 ng, 10 ng, 1.0 ng, 0.1 ng, and 0.01 ng of B31 A3 B. burgdorferi genomic DNA and the amount of each gene transcript calculated. The recA transcript was used as the endogenous reference to which the transcripts of the other genes were normalized. The bbk46 primers were confirmed to be specific for their gene target. Three biological replicate samples were analyzed in triplicate and normalized to recA mRNA. The data were reported as the average gene transcript/recA transcript for each sample. The amplification of samples lacking reverse transcriptase was similar to that of the no-template control.
Mouse infection experiments
Unless otherwise noted, groups of 6–8 week old C3H/HeN female mice (Harlan) were used for all experiments.
ID50 analysis of mammalian-adapted spirochetes.
A single C3H/HeN SCID mouse (Harlan) was inoculated with 2×106 B. burgdorferi B31 A3. Two weeks post infection the infected blood was harvested and used to inoculate groups of six wild-type C3H/HeN (Harlan) mice with 100 µl of undiluted infected blood or 100 µl of infected blood diluted 1∶10 or 1∶100 in BSK-H medium. The number of live spirochetes in the infected blood and therefore the actual spirochete dose in the inoculum was determined by plating the blood in solid BSK medium and quantitating the number of colony forming units (Table 1). In addition, groups of six wild-type C3H/HeN mice (Harlan) were inoculated with 5×104, 5×103, 5×102, 5×101 or 5×100, in vitro grown spirochetes at mid-log phase. The in vitro grown spirochetes were confirmed to harbor all plasmids required for infectivity .
Functional validation of the BbIVET system.
Groups of 6 mice were needle inoculated as described  with 1×104 spirochetes of clone A3 68-1 carrying pBbIVET or pBbIVET-ospCp. Mouse infection was assessed 3 weeks post inoculation by reisolation of spirochetes from ear, bladder and joint tissues as previously described , .
Gene expression studies.
Three mice were needle-inoculated intradermally under the skin of the upper back with B. burgdorferi clone B31 A3 at a dose of 1×105 spirochetes. Three weeks post inoculation mouse infection was determined by serology ,  and bladders harvested for RNA isolation.
bbk46 mutant infectivity studies.
Groups of five mice were needle-inoculated, intradermally under the skin of the upper back, with B. burgdorferi clones wild-type (A3-68ΔBBE02 ), Δbbk46/vector or Δbbk46/bbk46+ at a dose of 1×104 spirochetes. The number of spirochetes inoculated into mice was determined using a Petroff-Hausser counting chamber and verified by colony-forming unit (cfu) counts in solid BSK medium. Twelve colonies per inoculum were verified by PCR for the presence of the virulence plasmids lp25, lp28-1 and lp36 in at least 90% of the individuals in the population. Further, total plasmid content of each inoculum was confirmed to be as expected , , . Mouse infection was assessed three weeks post inoculation by serology using total B. burgdorferi lysate, as previously described  and 300 ng recombinant GST-OspC , as previously described . Spirochetes were reisolated from the inoculation site, ear, bladder and joint tissues, as previously described .
Groups of five immunodeficient C3SnSmn.CB17-Prkdc<scid>/J (Jackson labs stock 001131) were needle-inoculated with B. burgdorferi clones wild-type (A368ΔBBE02 ), Δbbk46/vector or Δbbk46/bbk46+ at a dose of 1×104 spirochetes. 80% of the inoculum was delivered intraperitoneal and 20% of the inoculum was delivered subcutaneous. The inoculum cultures were analyzed as described above. Mouse infection was assessed three weeks post inoculation by reisolation of spirochetes from ear, bladder and joint tissues .
Representative restriction digest analysis of individual pBbIVET plasmids rescued in E. coli. Colony PCR to amplify the in vivo-expressed DNA fragment was performed on a random subset of twenty four E. coli transformants carrying the rescued pBbIVET plasmids from infected mouse tissues. The PCR products were digested with a cocktail of the restriction enzymes DraI, SspI and AseI and separated on a 1% agarose gel. Numbers across the top of each image identify each non-identical restriction digestion pattern detected for the amplified pBbIVET DNA fragments. Representative data from two mouse tissues (A) and (B) are shown. Migration of the DNA ladders is shown in base pairs on both sides of each image. NTC, PCR no template control.
The BBK46 protein is non-immunogenic in mice. Recombinant GST-BBK46 and GST alone produced in and purified from E. coli, along with total protein lysate from E. coli and B. burgdorferi (Bb lysate) and E. coli producing the B. burgdorferi antigen BmpA (+) were separated by SDS-PAGE and transferred to a nitrocellulose membrane. Immunoblot analysis was performed using immune serum collected from mice infected with wild-type B. burgdorferi and anti-GST monoclonal antibodies (α GST). The positions of markers to the left of the panel depict protein standard molecular masses in kilodaltons.
Many thanks to Dr. Andrew Camilli for sharing his expertise and to members of the P. Rosa lab for their helpful suggestions during the initial stages of developing IVET for B. burgdorferi. Thank you to Dr. Daniel Dulebohn for sharing his in vitro temperature-shift induction method. Thank you to Selina Sutchu for technical assistance. Thank you to Dr. Travis Jewett for insightful comments and continued support. Thank you to the UCF NAF animal care staff.
Conceived and designed the experiments: TCE MWJ. Performed the experiments: TCE SJ AKL KR AB MH SK MWJ. Analyzed the data: TCE SJ AKL MWJ. Contributed reagents/materials/analysis tools: TCE SJ AKL KR PAR MH SK MWJ. Wrote the paper: TCE MWJ.
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