Citation: Bayry J, Aimanianda V, Guijarro JI, Sunde M, Latgé J-P (2012) Hydrophobins—Unique Fungal Proteins. PLoS Pathog8(5): e1002700. https://doi.org/10.1371/journal.ppat.1002700
Editor: Joseph Heitman, Duke University Medical Center, United States of America
Published: May 31, 2012
Copyright: © 2012 Bayry et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by ANR-10-Blanc SVSE 3-009 HYDROPHOBIN; European Community's Seventh Framework Programme [FP7/2007-2013] under Grant Agreement No: 260338 ALLFUN; and the Commonwealth of Australia under the International Science Linkages program (French-Australian Science & Technology Program 2011). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Microorganisms are often covered by a proteinaceous surface layer that serves as a sieve for external molecular influx, as a shield to protect microbes from external aggression, or as an aid to help microbial dispersion. In bacteria, the latter is called the S-layer, in Actinomycetes, the rod-like fibrillar layer, and in fungi, the rodlet layer . The self-assembly properties and remarkable structural and physicochemical characteristics of hydrophobin proteins underlie the multiple roles played by these unique proteins in fungal biology.
What Are Hydrophobins?
Hydrophobins, low molecular mass (≤20 kDa) secreted proteins of fungi, are characterized by moderate to high levels of hydrophobicity and the presence of eight conserved cysteine (Cys) residues. These proteins are able to assemble spontaneously into amphipathic monolayers at hydrophobic–hydrophilic interfaces. Although functional homologues are reported in Streptomyces (chaplins, SapB, and SapT for aerial morphogenesis; ), hydrophobins are unique to the fungal kingdom. Fungal genome analyses have indicated that hydrophobins generally exist as small gene families with two to ten members, although certain species contain more members (e.g., Coprinus cinereus displays 33 members; http://www.broadinstitute.org) , . Hydrophobins show very little sequence conservation in general, apart from the idiosyncratic pattern of eight Cys residues implicated in the formation of four disulfide bridges (Cys1–Cys6, Cys2–Cys5, Cys3–Cys4, Cys7–Cys8)  (Figure 1). Based on hydropathy plots, solubility and the type of layer they form, hydrophobins are divided into two classes [6, Reference S1 in Text S1], although recent bioinformatics studies suggest that intermediate/different forms can also exist and that many hydrophobins with distinct physicochemical characteristics may have been overlooked in the past , . In class I, considerable variation is seen in the inter-Cys-spacing; these hydrophobins assemble into highly insoluble polymeric monolayers composed of fibrillar structures known as rodlets. The rodlets are extremely stable, can only be solubilized with harsh acid treatments, and the soluble forms can polymerize back into rodlets under appropriate conditions. Despite the low sequence similarity, class I hydrophobins from different fungal species could partially complement a Magnaporthe grisea class I hydrophobin gene (MPG1) deletion mutant, suggesting that hydrophobins constitute a closely related group of morphogenetic proteins . The sequence and the inter-Cys spacing are more conserved in class II; the monolayers formed by class II hydrophobins lack the fibrillar rodlet morphology and can be solubilized with organic solvents and detergents.
Fungal hydrophobins are unique amphipathic proteins with multiple roles in the fungal life cycle and in mediating interactions between fungus and host. There is diversity in the primary sequences of hydrophobins but they share a similar core three-dimensional structure and a pattern of four disulfide bonds (shown in amber) that stabilize the structures. Increasingly, these proteins show potential for modification of hydrophobic nanomaterials and in solubilizing lipophilic drugs.
Hydrophobins at the Interface in the Fungal Life Cycle
Fungi are heterotrophic terrestrial eukaryotes, showing two types of growth morphologies: unicellular yeast and multicellular filamentous forms. Yeasts are hydrophilic and they lack hydrophobins. The vegetative hyphae of filamentous fungi growing on moist environments are also hydrophilic and do not show the presence of rodlets on their surface. In contrast, the aerial hyphae and the asexual spores (conidia) are hydrophobic, due to the presence of hydrophobins. The functions of hydrophobins are related to their high surfactant activity, which results from their self-assembly at hydrophilic–hydrophobic interfaces to form an amphipathic monolayer. The hydrophobin layer reduces the surface tension of the medium or the substratum in/on which fungi grow, allowing them to breach the air–water interface or preventing water-logging while maintaining permeability to gaseous exchange . Spores produced on the aerial structures of filamentous fungi are covered by a hydrophobin rodlet layer that renders the conidial surface hydrophobic and wet-resistant, thus facilitating spore-dispersal in the air. The rodlet-forming hydrophobins are essential for these fungi to complete their biological cycle. In many “wet” fungi (e.g., Conidiobolus obscurus), the rodlet-layer is covered by a mucilaginous extracellular matrix that helps the conidia to bind to the substrate, and once the spores are bound to the host, the rodlet-layer is unmasked for better resistance to the environment . In the basidiomycete Agaricus bisporus, the hydrophobin HypA, found in the peel tissue of the mushroom cap, is suggested to form a protective layer during fruiting body development . In Cryphonectria parasitica, the deletion of the gene coding the class II hydrophobin cryparin generated a mutant incapable of erupting through the bark of the tree . Hydrophobins are also reported to play a role in the surface interaction during infection-related development of M. grisea [13, Reference S2 in Text S1]. In the symbiotic phenotypes of lichen-forming ascomycetes Xanthoria spp., the continuous rodlet-layer seals the apoplast continuum .
Structure of Hydrophobins
Hydrophobins from both classes have been studied in vitro and have been shown to be highly surface active and to form amphipathic monolayers on hydrophobic/hydrophilic surfaces. The crystal structures of the class II hydrophobins HFBI and HFBII from Trichoderma reesei have been solved , . In addition, the structure of the class I EAS protein from Neurospora crassa has been determined by NMR . These studies indicate that all hydrophobins share a similar small β-structured core that is dictated by the presence of the four disulfide bonds and that the proteins have large exposed hydrophobic surface regions that give rise to their high surface activity. The structures of the class I hydrophobins DewA (Aspergillus nidulans) and Mpg1 (M. grisea) and the class II hydrophobin from N. crassa, as well as the secondary structure of the class I hydrophobins RodA and RodB from Aspergillus fumigatus obtained through the analysis of their backbone NMR chemical shifts, are consistent with this (J. I. Guijarro and M. Sunde, unpublished data). Monolayer formation by class II hydrophobins does not appear to be associated with major conformational changes. In contrast, biophysical analysis of SC3 from S. commune and EAS indicate that rodlet formation is associated with significant structural rearrangements, in some cases involving helical intermediates, but always to a final rodlet form with high β-sheet content and amyloid characteristics , [17, Reference S3 in Text S1]. Digestion and hydrogen-deuterium exchange experiments with SC3  indicated that the Cys3–Cys4 loop is important for adhesion to hydrophobic surfaces and may directly participate in the formation of rodlets. However, truncation  and systematic site-directed mutagenesis  experiments with EAS have shown that the Cys3–Cys4 loop is not involved in rodlet formation and that the Cys7–Cys8 loop region is crucial for auto-assembly, suggesting that the variability of the sequences of class I hydrophobins may translate into different mechanisms of rodlet formation . Nevertheless, the surface tension seems to be the driving force to recruit class I hydrophobins to the air–water interface where the structural changes from the soluble form to the rodlet conformation take place .
Hydrophobins and Fungus–Host Interactions
The surface rodlet-layer has a critical role in masking the immunogenicity of airborne fungal spores . By covering the spore surface, the rodlet-layer imparts immunological inertness to the spores and ensures that pathogen-associated molecular patterns (PAMPs) are not recognized by innate and adaptive immune cells, thus preventing the activation of host immune system, inflammation, and tissue damage , , , [25, Reference S4 in Text S1]. Several lines of evidence suggest that the rodlet-layer, which covers the spores of both pathogenic and non-pathogenic fungal species, prevents immune recognition , ,  (Figure 1). In opportunistic pathogen A. fumigatus, the rodlet-layer made up of RodA imparts resistance to NETosis (a process associated with disruption of neutrophil-membranes and release of a mixture of nuclear DNA with a granular content that acts as a neutrophil extracellular trap [NET]) and killing by alveolar macrophages , . However, removal of RODA and RODB did not affect pathogenicity of A. fumigatus [Reference S5 in Text S1].
In plant-/entomo-pathogenic fungi, hydrophobins are also described as pathogenicity factors, but their precise role in fungal virulence remains to be understood. In the rice blast fungus M. grisea, the hydrophobin Mpg1 is suggested to function as a developmental sensor for appresorium formation, since it is involved in the interaction with hydrophobic leaf surfaces necessary for establishing the pathogenicity . Deletion of the MPG1 gene resulted in a mutant of M. grisea with reduced virulence; the deletion of another hydrophobin gene in M. grisea, MHP1, led also to a loss of viability and a reduced capacity to infect and colonize a susceptible rice cultivar . In Beauveria bassiana, the non-specific hydrophobic interaction between the fungal spore coat hydrophobin and the insect epicuticle is involved in establishing the pathogenicity of the fungus .
Prospective Applications of Hydrophobins
The potential applications of hydrophobins rely on their ability to reverse the hydrophilic-hydrophobic character of a surface and/or their surfactant capacity. Several biotechnological applications of hydrophobins have been proposed [29, Reference S6–S12 in Text S1]. However, the large-scale applications of hydrophobins might be difficult to implement due to the production cost of recombinant proteins and/or the large-scale requirements of the proteins. In contrast, in the pharmaceutical or in the nanotechnology industry, where the returns of investment are high, it is possible to envisage a potential development for these proteins. For example, the foam and air-/oil-filled emulsion-forming capacity of hydrophobins has been exploited in protecting nanoparticles and drug formulations [30, Reference S13–S16 in Text S1] (Figure 1). From a therapeutic point of view, the degradation-resistance and immunologically inert properties of hydrophobins could be used to generate hydrophobin-based nanoparticles with embedded therapeutic proteins and molecules that have to be slowly released within the host or transported to a specific body location without being recognized by the host immune system.
Many questions, however, remain unsolved in the study of hydrophobins: for instance, how is the 3D rodlet-structure organized? How are hydrophobins transported to the cell surface? How is the rodlet-layer attached to the spore surface? What are the signals that trigger germination of the spores covered by a rodlet layer? Addressing these questions will reveal the mechanism by which hydrophobins accomplish their multiple roles in the fungal life cycle.
- 1. Wessels J, De Vries O, Asgeirsdottir SA, Schuren F (1991) Hydrophobin Genes Involved in Formation of Aerial Hyphae and Fruit Bodies in Schizophyllum. Plant Cell 3: 793–799.J. WesselsO. De VriesSA AsgeirsdottirF. Schuren1991Hydrophobin Genes Involved in Formation of Aerial Hyphae and Fruit Bodies in Schizophyllum.Plant Cell3793799
- 2. Kodani S, Lodato MA, Durrant MC, Picart F, Willey JM (2005) SapT, a lanthionine-containing peptide involved in aerial hyphae formation in the streptomycetes. Mol Microbiol 58: 1368–1380.S. KodaniMA LodatoMC DurrantF. PicartJM Willey2005SapT, a lanthionine-containing peptide involved in aerial hyphae formation in the streptomycetes.Mol Microbiol5813681380
- 3. Sunde M, Kwan AH, Templeton MD, Beever RE, Mackay JP (2008) Structural analysis of hydrophobins. Micron 39: 773–784.M. SundeAH KwanMD TempletonRE BeeverJP Mackay2008Structural analysis of hydrophobins.Micron39773784
- 4. Littlejohna KA, Hooleyb P, Cox PW (2012) Bioinformatics predicts diverse Aspergillus hydrophobins with novel properties. Food Hydrocolloids 27: 503–516.KA LittlejohnaP. HooleybPW Cox2012Bioinformatics predicts diverse Aspergillus hydrophobins with novel properties.Food Hydrocolloids27503516
- 5. Kwan AH, Winefield RD, Sunde M, Matthews JM, Haverkamp RG, et al. (2006) Structural basis for rodlet assembly in fungal hydrophobins. Proc Natl Acad Sci U S A 103: 3621–3626.AH KwanRD WinefieldM. SundeJM MatthewsRG Haverkamp2006Structural basis for rodlet assembly in fungal hydrophobins.Proc Natl Acad Sci U S A10336213626
- 6. Wessels JG (1997) Hydrophobins: proteins that change the nature of the fungal surface. Adv Microb Physiol 38: 1–45.JG Wessels1997Hydrophobins: proteins that change the nature of the fungal surface.Adv Microb Physiol38145
- 7. Jensen BG, Andersen MR, Pedersen MH, Frisvad JC, Sondergaard I (2010) Hydrophobins from Aspergillus species cannot be clearly divided into two classes. BMC Res Notes 3: 344.BG JensenMR AndersenMH PedersenJC FrisvadI. Sondergaard2010Hydrophobins from Aspergillus species cannot be clearly divided into two classes.BMC Res Notes3344
- 8. Kershaw MJ, Wakley G, Talbot NJ (1998) Complementation of the mpg1 mutant phenotype in Magnaporthe grisea reveals functional relationships between fungal hydrophobins. EMBO J 17: 3838–3849.MJ KershawG. WakleyNJ Talbot1998Complementation of the mpg1 mutant phenotype in Magnaporthe grisea reveals functional relationships between fungal hydrophobins.EMBO J1738383849
- 9. Wang X, Shi F, Wosten HA, Hektor H, Poolman B, et al. (2005) The SC3 hydrophobin self-assembles into a membrane with distinct mass transfer properties. Biophys J 88: 3434–3443.X. WangF. ShiHA WostenH. HektorB. Poolman2005The SC3 hydrophobin self-assembles into a membrane with distinct mass transfer properties.Biophys J8834343443
- 10. Latgé JP, Cole GT, Horis berger M, Prévost MC (1986) Ultrastructure and chemical composition of the ballistospore wall of Conidiobolus obscurus. Exp Mycol 10: 99–113.JP LatgéGT ColeM. Horis bergerMC Prévost1986Ultrastructure and chemical composition of the ballistospore wall of Conidiobolus obscurus.Exp Mycol1099113
- 11. De Groot PW, Schaap PJ, Sonnenberg AS, Visser J, Van Griensven LJ (1996) The Agaricus bisporus hypA gene encodes a hydrophobin and specifically accumulates in peel tissue of mushroom caps during fruit body development. J Mol Biol 257: 1008–1018.PW De GrootPJ SchaapAS SonnenbergJ. VisserLJ Van Griensven1996The Agaricus bisporus hypA gene encodes a hydrophobin and specifically accumulates in peel tissue of mushroom caps during fruit body development.J Mol Biol25710081018
- 12. Kazmierczak P, Kim DH, Turina M, Van Alfen NK (2005) A hydrophobin of the chestnut blight fungus, Cryphonectria parasitica, is required for stromal pustule eruption. Eukaryot Cell 4: 931–936.P. KazmierczakDH KimM. TurinaNK Van Alfen2005A hydrophobin of the chestnut blight fungus, Cryphonectria parasitica, is required for stromal pustule eruption.Eukaryot Cell4931936
- 13. Talbot NJ, Kershaw MJ, Wakley GE, De Vries O, Wessels J, et al. (1996) MPG1 Encodes a fungal hydrophobin involved in surface interactions during infection-related development of Magnaporthe grisea. Plant Cell 8: 985–999.NJ TalbotMJ KershawGE WakleyO. De VriesJ. Wessels1996MPG1 Encodes a fungal hydrophobin involved in surface interactions during infection-related development of Magnaporthe grisea.Plant Cell8985999
- 14. Scherrer S, De Vries OM, Dudler R, Wessels JG, Honegger R (2000) Interfacial self-assembly of fungal hydrophobins of the lichen-forming ascomycetes Xanthoria parietina and X. ectaneoides. Fungal Genet Biol 30: 81–93.S. ScherrerOM De VriesR. DudlerJG WesselsR. Honegger2000Interfacial self-assembly of fungal hydrophobins of the lichen-forming ascomycetes Xanthoria parietina and X. ectaneoides.Fungal Genet Biol308193
- 15. Hakanpaa J, Paananen A, Askolin S, Nakari-Setala T, Parkkinen T, et al. (2004) Atomic resolution structure of the HFBII hydrophobin, a self-assembling amphiphile. J Biol Chem 279: 534–539.J. HakanpaaA. PaananenS. AskolinT. Nakari-SetalaT. Parkkinen2004Atomic resolution structure of the HFBII hydrophobin, a self-assembling amphiphile.J Biol Chem279534539
- 16. Hakanpaa J, Szilvay GR, Kaljunen H, Maksimainen M, Linder M, et al. (2006) Two crystal structures of Trichoderma reesei hydrophobin HFBI–the structure of a protein amphiphile with and without detergent interaction. Protein Sci 15: 2129–2140.J. HakanpaaGR SzilvayH. KaljunenM. MaksimainenM. Linder2006Two crystal structures of Trichoderma reesei hydrophobin HFBI–the structure of a protein amphiphile with and without detergent interaction.Protein Sci1521292140
- 17. de Vocht ML, Reviakine I, Ulrich WP, Bergsma-Schutter W, Wosten HA, et al. (2002) Self-assembly of the hydrophobin SC3 proceeds via two structural intermediates. Protein Sci 11: 1199–1205.ML de VochtI. ReviakineWP UlrichW. Bergsma-SchutterHA Wosten2002Self-assembly of the hydrophobin SC3 proceeds via two structural intermediates.Protein Sci1111991205
- 18. Wang X, Permentier HP, Rink R, Kruijtzer JA, Liskamp RM, et al. (2004) Probing the self-assembly and the accompanying structural changes of hydrophobin SC3 on a hydrophobic surface by mass spectrometry. Biophys J 87: 1919–1928.X. WangHP PermentierR. RinkJA KruijtzerRM Liskamp2004Probing the self-assembly and the accompanying structural changes of hydrophobin SC3 on a hydrophobic surface by mass spectrometry.Biophys J8719191928
- 19. Kwan AH, Macindoe I, Vukasin PV, Morris VK, Kass I, et al. (2008) The Cys3–Cys4 loop of the hydrophobin EAS is not required for rodlet formation and surface activity. J Mol Biol 382: 708–720.AH KwanI. MacindoePV VukasinVK MorrisI. Kass2008The Cys3–Cys4 loop of the hydrophobin EAS is not required for rodlet formation and surface activity.J Mol Biol382708720
- 20. Macindoe I, Kwan AH, Ren Q, Morris VK, Yang W, et al. (2012) Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS. Proc Natl Acad Sci U S A 109: E804–E811.I. MacindoeAH KwanQ. RenVK MorrisW. Yang2012Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS.Proc Natl Acad Sci U S A109E804E811
- 21. Morris VK, Ren Q, Macindoe I, Kwan AH, Byrne N, et al. (2011) Recruitment of class I hydrophobins to the air:water interface initiates a multi-step process of functional amyloid formation. J Biol Chem 286: 15955–15963.VK MorrisQ. RenI. MacindoeAH KwanN. Byrne2011Recruitment of class I hydrophobins to the air:water interface initiates a multi-step process of functional amyloid formation.J Biol Chem2861595515963
- 22. Aimanianda V, Bayry J, Bozza S, Kniemeyer O, Perruccio K, et al. (2009) Surface hydrophobin prevents immune recognition of airborne fungal spores. Nature 460: 1117–1121.V. AimaniandaJ. BayryS. BozzaO. KniemeyerK. Perruccio2009Surface hydrophobin prevents immune recognition of airborne fungal spores.Nature46011171121
- 23. Bruns S, Kniemeyer O, Hasenberg M, Aimanianda V, Nietzsche S, et al. (2010) Production of extracellular traps against Aspergillus fumigatus in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA. PLoS Pathog 6: e1000873.S. BrunsO. KniemeyerM. HasenbergV. AimaniandaS. Nietzsche2010Production of extracellular traps against Aspergillus fumigatus in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA.PLoS Pathog6e1000873
- 24. Hohl TM, Van Epps HL, Rivera A, Morgan LA, Chen PL, et al. (2005) Aspergillus fumigatus triggers inflammatory responses by stage-specific beta-glucan display. PLoS Pathog 1: e30.TM HohlHL Van EppsA. RiveraLA MorganPL Chen2005Aspergillus fumigatus triggers inflammatory responses by stage-specific beta-glucan display.PLoS Pathog1e30
- 25. Dagenais TR, Giles SS, Aimanianda V, Latge JP, Hull CM, et al. (2010) Aspergillus fumigatus LaeA-mediated phagocytosis is associated with a decreased hydrophobin layer. Infect Immun 78: 823–829.TR DagenaisSS GilesV. AimaniandaJP LatgeCM Hull2010Aspergillus fumigatus LaeA-mediated phagocytosis is associated with a decreased hydrophobin layer.Infect Immun78823829
- 26. Paris S, Debeaupuis JP, Crameri R, Carey M, Charles F, et al. (2003) Conidial hydrophobins of Aspergillus fumigatus. Appl Environ Microbiol 69: 1581–1588.S. ParisJP DebeaupuisR. CrameriM. CareyF. Charles2003Conidial hydrophobins of Aspergillus fumigatus.Appl Environ Microbiol6915811588
- 27. Kim S, Ahn IP, Rho HS, Lee YH (2005) MHP1, a Magnaporthe grisea hydrophobin gene, is required for fungal development and plant colonization. Mol Microbiol 57: 1224–1237.S. KimIP AhnHS RhoYH Lee2005MHP1, a Magnaporthe grisea hydrophobin gene, is required for fungal development and plant colonization.Mol Microbiol5712241237
- 28. Zhang S, Xia YX, Kim B, Keyhani NO (2011) Two hydrophobins are involved in fungal spore coat rodlet layer assembly and each play distinct roles in surface interactions, development and pathogenesis in the entomopathogenic fungus, Beauveria bassiana. Mol Microbiol 80: 811–826.S. ZhangYX XiaB. KimNO Keyhani2011Two hydrophobins are involved in fungal spore coat rodlet layer assembly and each play distinct roles in surface interactions, development and pathogenesis in the entomopathogenic fungus, Beauveria bassiana.Mol Microbiol80811826
- 29. Linder MB, Szilvay GR, Nakari-Setala T, Penttila ME (2005) Hydrophobins: the protein-amphiphiles of filamentous fungi. FEMS Microbiol Rev 29: 877–896.MB LinderGR SzilvayT. Nakari-SetalaME Penttila2005Hydrophobins: the protein-amphiphiles of filamentous fungi.FEMS Microbiol Rev29877896
- 30. Valo HK, Laaksonen PH, Peltonen LJ, Linder MB, Hirvonen JT, et al. (2010) Multifunctional hydrophobin: toward functional coatings for drug nanoparticles. ACS Nano 4: 1750–1758.HK ValoPH LaaksonenLJ PeltonenMB LinderJT Hirvonen2010Multifunctional hydrophobin: toward functional coatings for drug nanoparticles.ACS Nano417501758