Like many organisms the fungal pathogen Candida albicans senses changes in the environmental CO2 concentration. This response involves two major proteins: adenylyl cyclase and carbonic anhydrase (CA). Here, we demonstrate that CA expression is tightly controlled by the availability of CO2 and identify the bZIP transcription factor Rca1p as the first CO2 regulator of CA expression in yeast. We show that Rca1p upregulates CA expression during contact with mammalian phagocytes and demonstrate that serine 124 is critical for Rca1p signaling, which occurs independently of adenylyl cyclase. ChIP-chip analysis and the identification of Rca1p orthologs in the model yeast Saccharomyces cerevisiae (Cst6p) point to the broad significance of this novel pathway in fungi. By using advanced microscopy we visualize for the first time the impact of CO2 build-up on gene expression in entire fungal populations with an exceptional level of detail. Our results present the bZIP protein Rca1p as the first fungal regulator of carbonic anhydrase, and reveal the existence of an adenylyl cyclase independent CO2 sensing pathway in yeast. Rca1p appears to regulate cellular metabolism in response to CO2 availability in environments as diverse as the phagosome, yeast communities or liquid culture.
Skin infection, oral and vaginal thrush, or bloodstream candidiasis are some of the diseases caused by the human pathogen Candida albicans. The high versatility of infection niches reflects the capacity of this yeast to respond to strong variations in its environment such as CO2 concentration. This molecule initiates the regulation of an essential protein: carbonic anhydrase, not through the known adenylyl cyclase CO2 sensor but as we discovered via a novel fungal CO2 sensing pathway involving the transcriptional regulator Rca1p. This protein is additionally implicated in growth, yeast-to-hyphae morphological switch and cell wall stability of C. albicans. The ortholog of Rca1p in Saccharomyces cerevisiae demonstrated a conserved function in the induction of the carbonic anhydrase in low CO2 concentration atmospheres pointing to the broad significance of Rca1p in fungal CO2 sensing.
Citation: Cottier F, Raymond M, Kurzai O, Bolstad M, Leewattanapasuk W, Jiménez-López C, et al. (2012) The bZIP Transcription Factor Rca1p Is a Central Regulator of a Novel CO2 Sensing Pathway in Yeast. PLoS Pathog8(1): e1002485. https://doi.org/10.1371/journal.ppat.1002485
Editor: Leah E. Cowen, University of Toronto, Canada
Received: July 11, 2011; Accepted: November 29, 2011; Published: January 12, 2012
Copyright: © 2012 Cottier et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was funded by the BBSRC, and the MRC (all FAM), WL was the recipient of a postgraduate training grant from the Royal Thai Fellowship Program, by the Ministry of Education of the Czech Republic LC531, MSM0021620858 and AV0Z50200510 and Howard Hughes Medical Institute International Research Award to ZP. MR was funded by a grant from the Canadian Institutes of Health Research (MOP-84341), and MCL was supported by NIH award R21AI071134. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Atmospheric carbon dioxide (CO2) with a concentration of 0.039% is not only central to the Earth's biogeochemical carbon cycle but is also sensed as a signal by many organisms. The nematode and parasite of insects Neoaplectana carpocapsae localizes its prey via a CO2 gradient , while avoidance behaviour in another nematode, Caenorhabditis elegans , or the model organism Drosophila melanogaster is provoked by elevated CO2 . C. elegans detects CO2 via a cGMP-gated ion channel  whereas in D. melanogaster CO2 is sensed by a pair of 7 transmembrane domains chemoreceptors localized on specialized sensory neurons .
In the fungal kingdom CO2, under its hydrated form bicarbonate (HCO3−), is critical for cellular metabolism. Although hydration of CO2 to HCO3− and a proton occurs spontaneously, this reaction is greatly enhanced by the metalloenzyme Carbonic Anhydrase (CA), which operates at a rate of up to 106 reactions per second . Fungal CAs fix the membrane permeable gas CO2 as HCO3− inside the cell, which is subsequently used as substrate for fundamental carboxylation reactions including the conversion of acetyl-CoA to malonyl-CoA (EC 18.104.22.168), or pyruvate to oxaloacetate (EC 22.214.171.124). The direct relevance of HCO3− synthesis for fungal survival is reflected by the fact that the CA deletion mutants of Candida albicans, Cryptococcus neoformans, Saccharomyces cerevisiae, Sordaria macrospora, Aspergillus fumigatus or Aspergillus nidulans fail to grow in ambient air , , , , . However, when cultured in a CO2−enriched atmosphere, where sufficient HCO3− is spontaneously formed to meet the metabolic requirements, CAs are optional.
In fungi CO2 is also sensed as a signal to regulate the expression of virulence factors. In the pathogenic yeast C. albicans, high level of CO2 triggers filamentous growth and the white-opaque switch , . Recently we have shown that in C. albicans CO2/HCO3− is detected by the enzyme adenylyl cyclase Cyr1p which regulates most processes considered essential in C. albicans virulence , . Here, Cyr1p senses CO2/HCO3− by a lysine residue (position 1373) of the C-terminal catalytic-site  potentially linking HCO3−, generated by CA, and cAMP signaling. In humans CAs are involved in medically relevant processes including bone calcification, or renal clear-cell-carcinoma progression; consequently, understanding their regulation and use of inhibitors has attracted considerable interest . This led to the identification of the first regulator of CA, the bHLH transcription factor HIF-1α, which controls the expression of major hypoxia-induced genes including CA IX . Another recently identified CA regulator is AphB from Vibrio cholera . This LysR-type transcription factor also activates the ToxR virulence cascade via the tcpPH operon which ultimately induces the production of cholera toxin.
Notably the CAs of S. cerevisiae , , S. macrospora , A. fumigatus and A. nidulans  are expressed in response to the availability of environmental CO2. However, fungal genomes do not posses orthologs of either HIF-1α or AphB-type CA regulators. This suggests the existence of an, as yet, undiscovered CO2 signaling mechanism controlling fungal CA expression.
In this report we investigate the existence of such a pathway in fungi by using, as a model, the well characterized CO2 sensing system of the pathogenic yeast C. albicans. We have shown that C. albicans posses a single β-CA, required for growth under CO2 limiting atmosphere . We now demonstrate that the expression of both transcript and protein of this CA is controlled by the level of environmental CO2 and that CA is further induced in an ex vivo model of phagocytosis by mammalian phagocytes, suggesting that CO2 might be limiting even in the relatively high CO2 conditions in the host. We find that such regulation in C. albicans is independent from the already known sensor adenylyl cyclase, described above, suggesting the existence of a cAMP-independent CO2 signaling pathway in fungi. By implementing a systematic functional screen we identify the bZIP transcription factor Rca1p as the C. albicans regulator of CA expression in response to CO2 availability. Furthermore, by using Chromatin Immuno Precipitation (ChIP) on chip and ChIP-qPCR experiments we confirm that Rca1p binds to the CA promoter, and to 84 additional genes. The broad significance of our findings is further underlined by our data revealing the existence of a conserved CO2 sensing pathway controlled by an Rca1p ortholog in the model organism S. cerevisiae. Finally, using advanced microscopy, we contribute to the understanding of CO2 flux and metabolic adaptation inside yeast populations on a hitherto unprecedented level of resolution.
Yeast carbonic anhydrases are expressed according to CO2 availability
The CA, Nce103p, from C. albicans and S. cerevisiae are known to be required for growth in ambient air (,  and Figure 1A, B). However, to allow an in-depth analysis of yeast CA expression we developed an antibody directed against C. albicans Nce103p, and additionally constructed a strain expressing a functional tagged CA in S. cerevisiae: Scnce103Δ+ScNCE103-GFP (Figure 1B).
Inactivation of the β-carbonic anhydrase encoded by NCE103 in (A) C. albicans, (B) S. cerevisiae inhibits growth in ambient air (right set of pictures) but not in air enriched with 5.5% CO2 (left set of pictures). All strains were incubated on YPD medium for 24 hours. C) Western blots from C. albicans (left) and S. cerevisiae (right). Yeast carbonic anhydase is present in higher quantity in air than air enriched with 5.5% CO2 samples. D) qRT-PCR using NCE103 specific primers and RNA extracted from C. albicans (left) and S. cerevisiae (right) grown in air (black columns) or air enriched with 5.5% CO2 (white columns). E) Western blot (top) and qRT-PCR (bottom) relative to C. albicans cyr1Δ. Data are represented as mean +/− SD. Asterisk indicates statistical significance determined by two-sample t test (P≤0.05).
Since the CAs from C. albicans and S. cerevisiae are required for growth in a low but not high CO2 atmosphere we asked whether expression of the enzyme itself is regulated by the availability of this gas. To address this question we performed Western-blot analysis using appropriate antibodies to detect C. albicans CA and CA chimera of S. cerevisiae. Single bands with the predicted molecular weights for CaNce103p (32kDa), and ScNce103-GFPp (54kDa) were detected and we now show for the first time that CA protein expression is highly regulated in both yeast species (Figure 1C). In fact, CA is strongly expressed when yeasts are grown in ambient air but non-detectable when cultured in air enriched with 5.5% CO2, precisely mirroring the requirements for growth of the NCE103 gene in yeast (Figure 1A, B). Furthermore, quantitative real time polymerase chain reaction (qRT-PCR) analysis with reverse transcripts of total RNA extracted from C. albicans and S. cerevisiae grown at low and high CO2 concentrations show a similar regulation of CA transcript when expressions were normalized to ACT1 (Figure 1D), confirming previous reports made in S. cerevisiae , .
CO2 regulation of carbonic anhydrases in yeast is independent of cAMP signaling
We have previously shown that the adenylyl cyclase Cyr1p from C. albicans functions as a major CO2-sensor, promoting the yeast-to-hyphae switch in response to high levels of CO2 , . We now asked if CO2 regulation of CA expression was similarly coordinated by Cyr1p or cAMP signaling examining CA protein and transcript levels in a strain where both alleles of CYR1 have been deleted (cyr1Δ). Notably, CO2 regulation of both protein and transcript levels of NCE103 remain unaltered in the cyr1Δ strain displaying a pattern of expression identical to the control strain CAI4+pSM2 (Figure 1E). Furthermore, Western-blot or qRT-PCR analysis revealed that supplementation of culture media with exogenous cAMP at concentrations known to mimic Cyr1p activity  (10 mM) did not affect CA expression in CAI4+pSM2 grown in ambient air (Figure S1). Similarly to C. albicans, addition to the growth media of 10 mM cAMP did not affect the expression of CA protein or transcript in S. cerevisiae (Figure S1). Taken together our data demonstrate that CO2 regulation of CA in C. albicans is independent of the known CO2 sensor Cyr1p and its product cAMP. Furthermore, they strongly support the existence of a novel, cAMP-independent, CO2 signaling pathway in yeast.
The bZIP transcription factor Rca1p is a new regulator of CO2 signaling in C. albicans
To identify the key components of this novel CO2 sensing pathway we systematically screened a C. albicans knock-out library searching for strains with an altered expression of their CA in response to CO2. The library consisted of 158 C. albicans non-essential transcription factor mutants (provided by D. Sanglard). CA protein expression was investigated in each mutant grown in either ambient air or air enriched with 5.5% CO2. Repeated screening identified a single candidate (HZY7-1) that failed to induce CA protein when grown in ambient air. HZY7-1 harbors a mutation in the C. albicans orf19.6102 gene. To confirm the HZY7-1 phenotype, we independently inactivated the two orf19.6102 alleles in a CAI4 background, using the URA-blaster approach , and re-introduced URA3 at its native locus to generate strain rca1Δ. Subsequent to validating gene inactivation by Southern blot and qRT-PCR (Figure S2 and S3), we confirmed a striking loss of Nce103p protein induction in rca1Δ in ambient air (Figure 2A). We also validated that during the inactivation process, we did not alter the expression of 2 genes partially overlapped by RCA1: orf19.6103 and MVD (Figure S3). In light of these findings we named the gene encoded by orf19.6102: Regulator of Carbonic Anhydrase 1. RCA1 encodes a 283 amino acid (aa) hypothetical protein which contains a conserved basic leucine zipper (bZIP) domain in its C-terminus, required for DNA interaction (Figure S4). Reintroduction of RCA1, either on its own (rca1Δ+RCA1) or tagged at its C-terminus with Haemagglutinin (rca1Δ+RCA1−HA3), into the rca1Δ strain restored CA protein induction in C. albicans cells exposed to low CO2 level (Figure 2A). These observations were also confirmed by qRT-PCR (Figure 2B).
A) Western blots with protein extract from the C. albicans control strain, rca1Δ and rca1 complemented strains. B) qRT-PCR using NCE103 specific primers and RNA extracted from the above strains grown in air (black columns) or air enriched with 5.5% CO2 (white columns). C) qRT-PCR with NCE103 specific primers were used to calculate the ratio of transcript between cells phagocyted and cells grown in vitro in the control (black column) or rca1Δ strains (white column). Data are represented as mean +/− SD. Asterisk indicates statistical significance determined by two-sample t test (P≤0.05).
C. albicans NCE103 induction in an ex vivo virulence model is dependent on Rca1p
In a previous study of transcriptional variation that follow phagocytosis of C. albicans by murine macrophages, NCE103 was found to be mildly induced (∼1.9-fold after two hours of co-culture , while in S. cerevisiae, NCE103 was one of the genes most highly induced by phagocytosis (13.8-fold; ). We assayed expression of CaNCE103 in phagocytosed C. albicans cells after one hour of co-culture by qRT-PCR and found an induction of 2.1-fold relative to cells in media alone, even though both populations were exposed to a high-CO2 environment (5.0% in a tissue culture incubator). This change is of similar magnitude, but slightly faster, than observed by microarray. This induction was completely absent in an rca1Δ strain (Figure 2C). These results indicate that Rca1p regulates CaNCE103 in a physiological environment which could be correlated to a CO2 concentration scarcer within the immune cell due to a limited penetration across multiple membranes, the sequestering activity of the mammalian CAs, or the reduced metabolic production of CO2 in the fungal cell as a result of a shift to slower, and gluconeogenic growth.
CO2 regulation of carbonic anhydrases by Rca1p orthologs is conserved in S. cerevisiae
Since CA expression in S. cerevisiae is also controlled by ambient CO2 levels we investigated the existence of Rca1p orthologs in this yeast. In S. cerevisiae, we identified Cst6p (BLAST; Score: 117; E value: 1e−16) as a potential Rca1p ortholog. Cst6p encodes for a putative 587 aa protein with a bZIP domain in the C-terminus (Figure S4). In order to prove that Cst6p is a yeast CA regulator we constructed the mutants in the S. cerevisiae ScNCE103-GFP background (ScNCE103−GFP+cst6Δ). Successful gene inactivations were confirmed by diagnostic PCR and qRT-PCR (Figure S2 and S3). Using anti-GFP antibodies for ScNCE103−GFP+cst6Δ we found that its CA, similar to the C. albicans rca1Δ strain (Figure 2A), was not induced in low ambient CO2 when compared to the controls (Figure 3A). This regulation in S. cerevisiae mutant was also confirmed at transcript level by qRT-PCR (Figure 3B). In the mutant, introduction of a plasmid expressing CST6 restores the expression of NCE103 in air (Figure 3A). Taken together these data show that CO2 regulation by Rca1p orthologs is conserved in yeast.
A) Western blot with extracts from S. cerevisiae ScNCE103−GFP+pRS316 control strain, cst6Δ mutant and the complemented strain (ScNCE103−GFP+cst6Δ+pRS316−CST6). B) qRT-PCR with specific primers were used to calculate the ratio of NCE103 transcript between low (air) and high CO2 (5.5%) in S. cerevisiae control strain (black column), cst6Δ mutant (white column) and point mutation in the promoter of NCE103 (Scnce103Δ+ScNCE103−GFP−MUT) (grey column). Data are represented as mean +/− SD. Asterisk indicates statistical significance determined by two-sample t test (P≤0.05).
CO2 regulation of NCE103 in S. cerevisiae occurs through a specific DNA binding motif
S. cerevisiae Cst6p is a transcription factor previously described to bind a specific DNA motif: TGACGTCA . We identified this motif in the NCE103 promoters of S. cerevisiae (position -285 bp to ATG), but not of C. albicans. To assess the role of this motif in CO2 regulation of CA expression we neutralized it by removing 7 and 4 bases pairs of the TGACGTCA sequence in the promoters controlling Nce103p expression in S. cerevisiae. Notably the resulting strains (Scnce103Δ+ScNCE103−GFP−MUT) failed to induce CA when exposed to low environmental CO2 (Figure 3B), exactly mirroring the expression pattern displayed by the S. cerevisiae cst6Δ mutants (Figure 3B). In summary, our data show that CO2 regulation of CAs expression in yeast is controlled by a conserved transcriptional factor, but involves divergent DNA motifs between S. cerevisiae and C. albicans.
ChIP-chip analysis of Rca1p confirms NCE103 binding and points to a wider role in C. albicans CO2 sensing
To confirm that Rca1p directly binds to the CA promoter, and identify any additional genes it controls, we performed Chromatin Immuno Precipitation on Chip (ChIP-Chip) in air and air enriched with 5.5% CO2. We introduced the HA-tagged RCA1 allele, described above, into the heterozygous RCA1 mutant (rca1Δ/RCA1). The resulting strain (rca1Δ/RCA1+RCA1−HA3) expressed one wild-type and the HA-tagged RCA1 copy. Next, we confirmed that CA levels in rca1Δ/RCA1+RCA1−HA3 and in control strain rca1Δ/RCA1+RCA1 were fully responsive to CO2 by Western blotting, using anti-Nce103 antibodies (Figure S5). Subsequently, genome-wide location profiling of Rca1-HA3p in low and high CO2 using C. albicans whole-genome oligonucleotide tiling arrays  produced a total of 182 binding peaks, when the experiment was carried out in air, and 140 in air enriched with 5.5% CO2 (log2-transformed pseudomedian signal intensity cutoff: 0.5; P≤0.01) (Table S1 and S2) including 61 common “hits” between the two conditions. In depth analysis revealed that 85 of the hits could directly be associated with ORFs (hits located within 1kbp before an ATG start codon) (Figure 4A). Notably, we identified significant enrichments of several consecutive probes localized in the promoter of NCE103 (between position −654 and −479bp before ATG) in samples extracted from cells grown in air but not in those supplemented with 5.5% CO2 (Figure 4B). In addition to the promoter region, Rca1p binding was also enriched in the coding region of NCE103 (Figure 4B). This binding profile has been previously reported for another bZIP transcriptional factor, Cap1p, and suggests binding of the protein to the transcriptional machinery . To confirm the association of Rca1p to the CA promoter, we performed ChIP in tagged and untagged strains grown in low and high CO2, followed by a qPCR with primers specifically designed to amplify the predicted binding region of Rca1p on the NCE103 promoter. As expected, we observed a 2.13 fold enrichment of this sequence in the tagged strain compared to the untagged strain in air, compared to only 1.12 fold in 5.5% CO2 (Figure 4C). These results show a significant association of Rca1p to the promoter of NCE103 in air compared to the high CO2 environment.
A) Venn diagram of the Rca1p associated genes. B) Rca1p binds to the NCE103 promoter. Representation of the normalized log2-transformed signal intensities of RCA1-HA3-tagged in air (top panel) and high CO2 (bottom panel) compared to the untagged strain versus the corresponding position of each signal on C. albicans genomic regions. Log2-transformed signal intensity values are indicated at the left of the y-axis, the reference is the value 0 (i.e., a binding ratio of 1). C) ChIP-qPCR of RCA1-HA3 tagged strain versus untagged control in air and a 5.5% CO2 environment normalized to ACT1 level with primers designed to amplify the above identified binding region of Rca1p on the NCE103 promoter. D) qRT-PCR carried out with primers for C. albicans CHT2 (top) and OCH1 (bottom) on total RNA extracted from the C. albicans control strain (black columns) and rca1Δ (white columns) grown in air. Data are represented as mean +/− SD. Asterisk indicates statistical significance determined by two-sample t test (P≤0.05).
With respect to the other Rca1p associated genes, forty four of the 85 hits were specific to ambient air samples, 19 to enriched CO2 and 22 shared between the two conditions (Figure 4A). Rca1p binding peaks were directly associated with 4 other putative transcription factor encoding genes (CTA24, TFB3, ZCF4 and ZCF22) and 2 genes involved in cell wall biosynthesis (CHT2 encoding a chitinase and OCH1 coding for a α-1,6-mannosyltransferase). Since both CHT2 and OCH1 are involved in C. albicans virulence we selected them to examine the predicted role of Rca1p on their expression by qRT-PCR (Figure 4D) , . Transcript levels of CHT2 and OCH1 were significantly higher in rca1Δ in air when compared to the control strain (Figure 4D). These data show that in addition to Rca1p's function as activator of CA expression in low CO2, this regulator can also operate as a repressor.
Remarkably, 46 of the 85 (54%) Rca1p associated genes are presently uncharacterized (Table S1 and S2). Although this observation precludes assigning a significant enrichment of genes to any cellular function, process, component (GO Term Finder, http://www.candidagenome.org/cgi-bin/GO/goTermMapper) or protein families (pfam), it suggests a broader involvement of Rca1p in CO2 sensing. A similar conclusion can be made following database searches with the TGACGTCA sequence involved in Cst6p binding which was retrieved in 49 promoters of S. cerevisiae genes. Analysis of both lists with GO Slim Mapper coupled to a chi-square test revealed a significant under-representation of genes in the process of RNA metabolic process (P-value: 0.0066) in C. albicans as well as in the response to chemical stimulus process for both C. albicans and S. cerevisiae (respectively P-value 0.0436 and 0.0322) while the latter was over-represented in the budding yeast contrary to C. albicans. However, it is important to note that the number of genes involved was relatively low (respectively 2, 4 and 6). Altogether, these results show that, except for NCE103, no apparent commonality of putative Rca1p targets or pathways can be identified and the large number of uncharacterized genes in the two lists of genes poses limitations to the full elucidation of the impact of these transcriptional factors on yeast cell biology. At the same time, these data could point to the existence of yet undiscovered pathways and underline the intrinsic differences between the two fungal organisms. In summary, our data establish Rca1p as the first regulator of a fungal CA and imply a wider role of this transcription factor in a new fungal CO2 sensing pathway.
Rca1p regulation and role in growth, filamentation and cell wall biogenesis
Since CA is critical for yeast growth in air (Figure 1), and its induction depends on Rca1p, it can be predicted that inactivation of RCA1 should also result in a growth deficiency. Indeed we observed that rca1Δ has a 77% increase of its generation time compared to the control strain (Figure 5A). This phenotype is not restored in high CO2 pointing to a wider role of Rca1p in cell growth which could be set downstream of the CA. The enhanced growth rate of rca1Δ compared to nce103Δ is explained by residual expression of the highly effective carbonic anhydrase. We also confirmed that inactivation of RCA1 does not lead to significant morphological alterations (Figure S6).
A) Generation time in YPD of C. albicans (left panel) and S. cerevisiae (right panel) control strain (black columns) and RCA1 ortholog mutants (white columns) grown in air or 5.5% CO2 (grey columns). B) Germ tube formation in response to 5% serum of C. albicans control strain (black columns) and the rca1Δ (white columns) grown in air. C) Sensitivity assay of C. albicans control strain and rca1Δ. D) qRT-PCR using specific primers for CaRCA1 and CST6 on RNA extracted from C. albicans (top) and S. cerevisiae (bottom) control strains, CAI4+pSM2 and BY4741, grown in air (black columns) or air enriched with 5.5% CO2 (white columns). Data are represented as mean +/− SD. Asterisk indicates statistical significance determined by two-sample t test (P≤0.05).
Our ChIP-chip data suggest a connection of Rca1p to filamentous growth and cell wall biogenesis, an observation that we confirmed by showing a strong decrease in the morphological response of rca1Δ to serum (Figure 5B and S6) and an increased sensitivity of rca1Δ to Congo red, caffeine and SDS (Figure 5C). These results set Rca1p as an important player of C. albicans key biological functions.
In S. cerevisiae, we were not able to reach identical conclusions as inactivation of CST6 did not result in enhanced sensitivity to cell-wall perturbing agents. Additionally, only a 20% increase in generation time was observed for the cst6Δ mutant. Notably this phenotype was complemented by growing the strain in elevated CO2 (Figure 5A). These data confirm that the orthologs of RCA1 are involved in the regulation of different cell functions further underlining their intrinsic difference emerging from the ChIP-chip and bioinformatic analysis.
Interestingly expression levels of the Rca1p orthologues is also variable between the two species (Figure 5D). Using specific primers for each species (C. albicans and S. cerevisiae), we investigated the level of RCA1 and CST6 transcript in low and high CO2 environment. In C. albicans, RCA1 expression is 2.5 fold higher in hypercapnia compared to normal atmosphere (Figure 5D). In contrast, the CST6 transcript in S. cerevisiae did not display any significant variation of the expression between the two conditions (Figure 5D). While the function as a regulator of carbonic anhydrase is shared among Rca1p orthologs, their regulation in response to environmental CO2 differs.
Serine 124 is involved in the regulatory function of Rca1p
Sequence comparison of the Rca1p orthologs from C. albicans and S. cerevisiae identified three putative sites of phosphorylation (Figure S4). We investigated the role of these residues in the function of C. albicans Rca1p by complementation of rca1Δ with constructs expressing Rca1p with a replacement of serine to alanine in position 124 and 126 (rca1Δ+RCA1−S124A and rca1Δ+RCA1−S126A respectively) or serine to glycine in position 222 (rca1Δ+RCA1−S222G). Loss of serine in position 126 or 222 only partially impact on the CO2 regulation of Nce103p expression; however mutating serine 124 lead to a striking unresponsiveness to ambient CO2 resulting in enhanced expression of Nce103p in both air and air enriched with 5.5% CO2 (Figure 6). Our results point to a critical role of serine 124 for Rca1p activity in response to CO2 concentrations.
Carbonic anhydrase is differentially expressed in yeast populations
We have previously shown that in C. albicans colonies metabolically-generated CO2 accumulates and is subsequently used to activate the adenylyl cyclase Cyr1p promoting the switch from yeast to filamentous growth essential for pathology . We now substantially expand these results to entire populations of S. cerevisiae taking advantage of the regulation of expression of ScNce103-GFPp by CO2. Matching CA protein expression detected by Western blots (Figure 2A), a strong fluorescent signal was recorded in ScNCE103-GFP cells grown in ambient air but absent in air enriched with 5.5% CO2 (Figure 7A). Next we visualized Nce103p expression not only in individual cells but an entire fungal colony, monitoring for the first time the flux of CO2 in a fungal population. Using high resolution two-photon excitation confocal microscopy  we examined a cross-section of a ScNCE103-GFP colony grown for 4 days on solid nutrient agar. We observed that cells in the superficial layers, exposed to the low CO2 concentrations found in ambient air, strongly express the Nce103-GFPp construct; while the internal layers of the colony do not show any significant fluorescence (Figure 7B). Strikingly when grown in a 5.5% CO2 atmosphere, this gradient was absent, and no fluorescence was observed at any position in the colony. Similarly, no fluorescence was seen in cst6Δ, regardless of the CO2 concentration or the position in the colony (Figure 7B), indicating that the abscence of GFP expression in the center of the ScNCE103-GFP colony grown in air was unlikely due to a lack of viability or metabolic activity of the corresponding cells. By contrast, our positive control constitutively expressing GFP displays homogenous fluorescence through the cross-section (Figure 7B). In conclusion, our data visualizing the flux of CO2 inside yeast populations are in full agreement with those generated by Western blot or qRT-PCR in single cells (Figure 1C, D). Furthermore, they illustrate, with a high level of detail, the capacity of yeast to generate CO2 enriched micro-environments and adjust metabolic expression in a population.
A) Pictures of S. cerevisiae ScNCE103-GFP cells grown in YPD for 4h in air (right panel) or air supplemented with 5% CO2 (left panel). Picture magnification x60, bar corresponds to 20 µm. B) Cross section of a ScNCE103-GFP colony grown in ambient air (first panel) or in air enriched with 5.5% CO2 (second panel), ScNCE103-GFP+cst6Δ (third panel) BY4741+pTEF-GFP (fourth panel) were grown in air. Bar corresponds to 100 µm.
Carbon dioxide is a major signal in all organisms ranging from humans to fungi , . CO2 regulates numerous phenotypes including virulence in the fungal pathogens of humans C. albicans or C. neoformans , . Here we demonstrate for the first time a novel, cAMP independent, CO2 sensing pathway in the yeast species C. albicans and S. cerevisiae. We report that at the core of this new sensing pathway lies a bZIP transcription factor, Rca1p in C. albicans and its ortholog Cst6p in S. cerevisiae. We show that Rca1p and its orthologs regulate the expression of a major enzyme involved in fungal metabolism, CA, in response to changes in ambient CO2 level. CAs catalyze the synthesis of HCO3−, an essential substrate for the cell's carboxylation reaction that sustains gluconeogenesis, ureagenesis or lipogenesis , . We hypothesize that CA is critically involved in cellular metabolism and a feedback loop involving Rca1p could exist to regulate its expression (Figure 8). Furthermore, as CA controls the level of HCO3−, the regulation of CA expression driven by cellular metabolism could have an indirect impact on the capacity of the cells to differentiate through activation of the cAMP-PKA pathway.
Hydration of CO2 into HCO3− inside the cytoplasm occurs naturally and via the carbonic anhydrase activity. HCO3− can enter two pathways: 1) regulating morphogenesis via the activation of the adenylyl cyclase (AC) which results in an increase of cAMP production and activation of the cAMP-PKA pathway 2) integrating metabolic demand which could signal to Rca1p the cells requirement for HCO3− production resulting in the regulation of carbonic anhydrase expression controlling HCO3− synthesis rate.
While HCO3− is an essential cofactor for cellular metabolism in all fungi tested, the fungal requirement for CA is conditional, depending on the environmental CO2 concentration. CA mutants will not grow in ambient air where CO2 is scarce but will thrive in niches where the atmosphere is enriched with this gas , , , , ; the higher concentration allows sufficient spontaneous hydration to HCO3− to serve as substrate for the above carboxylation reactions. Regulation of CA expression by CO2 has been reported for S. cerevisiae, S. macrospora, A. fumigatus and A. nidulans , , ,  and we extend these observations to C. albicans.
Though the regulation of CA by CO2 has been observed in many fungi, the current work is the first to report the identification of a fungal-specific CO2-responsive transcription factor, Rca1p in C. albicans and of one of its orthologs in S. cerevisiae. We identified Rca1p, a previously uncharacterized bZIP-family DNA binding protein, via a functional genetic screen of a transcription factor knockout library. Rca1p functions by inducing CA protein and transcript when C. albicans faces low ambient CO2 level. Loss of CA induction in high CO2 level could result from a phosphorylation or another posttranslational modification on serine 124 which leads to the inability of Rca1p to bind NCE103 promoter, integrating Rca1p in a signal transduction pathway. The impact of Rca1p in cell growth and cell wall biogenesis, independently of CO2 concentration, points to a general involvement of Rca1p in the cellular metabolism of C. albicans. Rca1p function as CA regulator is conserved in S. cerevisiae, though they also have additional functions: Cst6p has already been shown to be involved in functions such as growth on non-optimal carbon sources , and our results now highlight the importance of Cst6p in cellular metabolism via its role as an inducer of CA. However, the impact of Cst6p on cell physiology differs when compared to C. albicans since the cts6Δ mutant growth defect in air is complemented by addition of environmental CO2 for S. cerevisiae. Furthermore the observation that RCA1 expression itself is regulated by CO2 underlines the importance of this regulator outside the scope of CA expression. Notably, Rca1p orthologs can be identified in S. macrospora, A. fumigatus and A. nidulans known to posses CA's which expression is influenced by ambient CO2 level , .
Importantly, Rca1p is distinct at both the sequence and functional level from the best characterized regulator of eukaryotic CAs, HIF-1α, which induces human CA IX expression in response to hypoxia , . CA IX leads to extracellular acidification of hypoxic tissue, and is as such abundant in tumors . By contrast C. albicans NCE103, is not regulated by either hypoxia or changes in pH (; Figure S7).
Similar to HIF-1α, which binds to the HRE motif present in CA IX promoter , the regulation of NCE103 by Rca1p appears to be direct. ChIP-chip and ChIP-qPCR confirmed that Rca1p binds to the CA promoter of C. albicans, specifically under low ambient CO2, leading to the induction of NCE103 expression. An additional 84 genes were associated with Rca1p, suggesting a much broader role of this new CO2 signaling pathway. Although the majority of these genes are currently of unknown function, in depth analysis of two (OCH1 and CHT2) showed Rca1p's potential to act as both an inducer and repressor of gene expression. This dual function could also be explained by an ability of Rca1p to recruit different co-factors at the associated loci. Using SCOPE  and other predictive programs, we obtained a relatively low number of results with significant value regarding binding sites or processes associated to Rca1p and Cst6p. These observations could be due to a higher complexity in the binding motif of Rca1p to the DNA, as well as on our limited knowledge about genes function (75% of ORF in C. albicans are still considered as uncharacterized; http://www.candidagenome.org). Reflecting the 235 million years  of separation between the Candida and Saccharomyces clade, we observed profound divergence in the associated genes of Rca1p and Cst6p. However, the CA remains a conserved target for both species. Such a fundamental wiring re-arrangement between closely related transcriptional factor of C. albicans and S. cerevisiae has already been reported .
In S. cerevisiae, CO2 regulation of CA expression by Cst6p involves the TGACGTCA palindrome motif. Database searches with this sequence retrieved the motif in 49 promoter regions of S. cerevisiae, and 40 promoters of C. albicans. While this motif is absent in the NCE103 promoter in C. albicans, it is present in a single gene associated to Rca1p (orf19.4246) demonstrating the lack of motifs conservation between the two species. Interestingly, the promoter of human CA IX presents a bZIP binding motif, TGAGTCA , which is closely related to the one identified in S. cerevisiae. Furthermore this motif is the binding sequence of the oncogen C-jun in human, which presents some sequence similarity with Rca1p (Score 42.7, e value 0.003), particularly around the bZIP domain. To date, the expression of CA IX in response to CO2 changes in the body has not been investigated. Our results may point to an additional level of regulation in human CA's; in fact HIF-1α sole predominance in CA IX regulation has recently been questioned .
The regulation of CA by CO2 is likely to be complex; however, using a new antibody that we generated, we show that CO2 affects CA proteins levels dramatically – highly induced in normal and undetectable when cells are grown in an elevated CO2 atmosphere. However, CA transcript levels were only decreased by 50% relative to ambient air. This type of regulation may suggest additional levels of post-transcriptional control on CA messenger. Maintaining CA transcripts in high CO2 level would allow a shortened response in CA enzyme synthesis when cells encounter a switch from high to low CO2 atmosphere, thus ensuring sufficient supply of the essential HCO3− ion.
We have begun to uncover the complex physiology associated with variations in CO2 concentrations. C. albicans cells phagocytosed by macrophages induce CA, as seen by qRT-PCR, despite being in a high CO2 (5%) environment, and this is Rca1p-dependent. This suggests that the phagocyte might restrict CO2 availability, as it does for other nutrients. Furthermore, using high resolution two-photon excitation confocal microscopy with GFP-tagged CA's we visualize for the first time the impact of CO2 build-up on gene expression in single cells but also in entire fungal populations. The data presented in this report not only confirm previous observations made in C. albicans that CO2 is compartmentalized in yeast populations, specifically inducing developmental change , but importantly connects micro-environments enriched in CO2 to metabolic specialization of individual members of a fungal populations. However, it is important to also consider the possibility that an unknown, CO2-independent, pathway is involved in the regulation of Nce103p at colony level. CA regulation is exquisitely sensitive to change in CO2 availability in both S. cerevisiae and C. albicans (Figure S8) and our results may be highly applicable to a range of conditions in which fungi expand and act as populations rather than individual cells including the formation of drug-resistant biofilms in pathogenic yeasts such as C. albicans. For S. cerevisiae, biofilms are naturally isolated on fruit surface (grape), and have a major application in industrial fermentation , .
The effects of CO2 on fungal physiology are integrated through more than one regulatory circuit. We previously showed that elevated CO2/HCO3− is sensed by the adenylyl cyclase via a lysine residue of the enzymes catalytic core, increasing the production of the second messenger cAMP, thus linking adenylyl cyclase and CA in fungal CO2 sensing , . Adenylyl cyclase/cAMP are particularly important mediators of fungal virulence determinants. Although there is cross-talk between the activities of the two enzymes, we now show that the CO2 control of CA expression acts independently from the cAMP-PKA pathway. This mechanism has to be compared to the identification of a cAMP-independent CO2-sensitive pathway involved in white opaque switching which results in Wor1p phosphorylation . However, the putative overlap of these two uncharacterized pathways remains to be defined.
In conclusion, carbon dioxide is sensed in yeast by two independent pathways. One, previously described by us, involves the fungal adenylyl cyclase and cAMP . We have now identified a second pathway and found the transcriptional regulator, Rca1p, at the core of the new pathway in yeast. Activation or inactivation of the transcription factor may involve phosphorylation which ultimately programs cellular metabolism to allow optimal adaptation to the environment inside a macrophage in the case of a fungal pathogen or yeast populations. Investigating the function of the additional Rca1p-associated genes will bring better understanding on how organisms sense the universal gas carbon dioxide.
Materials and Methods
All strains and plasmids used or constructed in this study are reported in supporting information (Table S3, S4, S5), as is the composition of the respective media and additional protocols (Protocol S1). C. albicans were incubated at 37°C, or 30°C for S. cerevisiae, either in ambient air or air enriched with 5.5% (vol/vol) atmospheric CO2 (Infors HT Minitron) when required.
Protein extraction and Western blot
Strains were inoculated in 50mL of YPD at OD600 0.1 and grown at the suitable temperature in air or air enriched with CO2. After 4h, cells were collected and quickly frozen. Samples were disrupted using a Mikro-dismembrator S (Sartorius) and resuspended in 500 µl lysis buffer (50 mM HEPES, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, protease inhibitor [Roche]). Protein concentrations were quantified using Bradford reagent (Sigma). 30 µg of protein were loaded for each sample on a 12% SDS-acrylamide/bis-acrylamide gel, and proteins were transferred to a PVDF membrane (Millipore). Membranes were incubated with the appropriate antibodies diluted as follows: anti-NCE103 at 1∶500, anti-LacZ at 1∶1000 (Millipore), anti-GFP at 1∶2500 (Roche) or anti-Act1p 1∶1000 (Sigma). This was followed by a second incubation with a peroxidase tagged antibodies of goat anti-rabbit, diluted 1∶2000, (Sigma) for the anti-NCE103, anti-LacZ and anti-Act1 primary antibodies, while a goat anti-mouse, diluted 1∶5000 (Sigma), was used for the anti-GFP primary antibody. Luminol Electrochemiluminescence was used to detect signal on the membrane.
Culture and samples were prepared in an identical manner as for the protein extraction, apart from total RNA extraction which was carried out with the RNeasy Kit (Qiagen) according to the manufacturer's recommendations. Transcripts level were determined by semi quantitative RT-PCR using the iScript One-Step RT-PCR Kit with Syber Green (BioRad). Levels were normalised to ACT1 from the respective species, and calculated using the Delta C(t) analysis of the Opticon Monitor 3 software (Bio-Rad). Values are represented as mean +/− SD from three independent experiments.
Chromatin Immuno Precipitation on Chip
50ml cultures in YPD of strains RCA1/rca1Δ+RCA1 (untagged) and RCA1/rca1Δ+RCA1−HA3 (tagged) were inoculated at OD600 0.1 by overnight culture and incubated 4h at 37°C in air or air enriched with 5.5% CO2, 140 rpm. Three independents cultures were grown for each strain in both conditions. The subsequent steps of DNA cross-linking, DNA shearing, chromatin immunoprecipitation (ChIP), DNA labeling with Cy3 or Cy5 dyes, hybridization to intergenic DNA microarrays, and data analysis were conducted exactly as described . Cy5-labeled DNA from the tagged strain (RCA1/rca1Δ+RCA1−HA3) and the corresponding Cy3-labeled DNA from the untagged control strain (RCA1/rca1Δ+RCA1) were mixed and hybridized to a C. albicans whole-genome tiled oligonucleotide DNA microarray . After hybridization and scanning of the slides (n = 3 for each condition), results were process . Quantile normalization was applied to the data . The parameters used were: a window size of 400 bp, a maximum genomic distance of 60 bp, and a minimum length of 120 bp. The replicate data were combined, and peak finding (i.e., determining the Rca1-HA3p binding sites) was done using a pseudomedian signal threshold of at least 1.5 fold and a P value cutoff of 0.01 , .
Chromatin Immuno Precipitations were processed according to the above protocol with the identical strains RCA1/rca1Δ+RCA1 (untagged) and RCA1/rca1Δ+RCA1−HA3 (tagged) and growth conditions. The resulting purified DNA was used in quantitative PCR using SYBR Green Master Mix (Applied Biosystems, Inc.) with primers: Ca-ChIP-NCE103-F/Ca-ChIP-NCE103-R for the detection of the NCE103 promoter (a 195bp region identified to be significantly associated with Rca1p) and Ca-ChIP-ACT1-F/Ca-ChIP-ACT1-R for the control ACT1 promoter, a gene without known association for Rca1p. Levels of detection were normalized to ACT1 and calculated using the Delta Delta C(t) method. Values are represented as mean +/− SD from two independent experiments.
Macrophage co-culture experiments
C. albicans wild-type (SC5314) and rca1Δ were grown to log-phase in YPD medium, washed in water, and counted. They were then incubated with J774A.1 macrophages at an MOI of 2∶1 (C. albicans: macrophages) in RPMI+10% FBS at 37°C in 5% CO2 in 750 cm3 vented flasks. Control cells were grown in the same media without macrophages at 37°C in 5% CO2. After incubation for one hour, the flasks were rinsed with PBS, then cells were collected by scraping into ice cold water and transferred to conical tubes. They were washed twice more with water, then pellets were frozen on dry ice. RNA was prepared using the Turbo DNA-free kit (Ambion). 50 ng of total RNA were used for each qRT-PCR reaction using the Power SYBR Green reaction system (Invitrogen). Actin (ACT1) was used as the normalization control. Primers are listed in supplemental data.
ScNCE103-GFP cells were observed with a Olympus IX-81 fluorescence microscope with a 150 W xenon-mercury lamp and an Olympus 60X Plan NeoFluor oil-immersion objective.
For high resolution two-photon excitation confocal microscopy of entire yeast colonies of ScNCE103-GFP, ScNCE103−GFP+cst6Δ and BY4741+pTEF−GFP, cells were grown for 4 days on YPD at 28°C. Colonies were then embedded in low-gelling agarose (Sigma-Aldrich) directly on the plates . After solidification, agarose-embedded colonies (an area of approximately 10×10 mm) were sectioned vertically down the middle and transferred to the cover glass. All samples on the cover slip were enclosed with a thick agarose layer to prevent them from drying. Image acquisition was realized following published protocol , using 20 x/0.7 water immersion planachromat objective.
Carbonic anhydrase expression is independent of the cAMP-PKA pathway. Using our anti-Nce103p and anti-GFP antibodies, carbonic anhydrase signals are shown in western blots from C. albicans (top) and S. cerevisiae (bottom). Proteins were extracted from cells grown in YPD for 4h in air (with or without addition of 10 mM dbcAMP to the culture medium) or air enriched with 5.5% CO2. Yeast carbonic anhydrase expression is not influenced by the addition of dbcAMP. The same samples were probed with an anti-actin antibody as control.
Strains verification in C. albicans and S. cerevisiae. A) Southern blot where genomic DNA from strain CAI4+pSM2 (1), rca1Δ+RCA1 (2), rca1Δ+RCA1−HA3 (3), rca1Δ (4), rca1Δ/RCA1+RCA1 (5 and 6) and rca1Δ/RCA1+RCA1−HA3 (7) were digested by SacI, migrated on agarose gel and transfered onto nitrocellulose membrane. Using a RCA1 probe, expected bands were observed with a signal at 3.8kbp for the RCA1 allele, 4.6kbp for rca1Δ, 8.1 and 8 kbp for the introduction of the pSM2-RCA1 and pSM2-RCA1-HA3 alleles respectively. B) Diagnostic PCR products with primers NCE103-Verif-F and ScNCE-end using genomic S. cerevisiae DNA of control strain BY4741 (lane 1) and Scnce103Δ (lane 3) as template, or primers Nce.ko.kan-F and NCE103-Verif-R with the respective template on lane 2 and 4. First set of primers hybridize on each side of the cassette, second set confirm presence of the cassette at the right locus. C) Diagnostic PCR products with primers CST6-Verif-F and CST6-Verif-R using genomic S. cerevisiae DNA of control strain ScNCE103-GFP (lane 1) and ScNCE103-GFP+cst6Δ (lane 3) as template, or primers ScCST6.ko.kan-F and CST6-Verif-R with the respective template on lane 2 and 4. First set of primers hybridize on each side of the cassette, second set confirm presence of the cassette at the right locus.
Verification of gene expression by qRT-PCR. A) qRT-PCR using RCA1 specific primers and RNA extracted from C. albicans control strain and the RCA1 mutant grown in air. The rca1Δ strains show no significant level of RCA1 transcript. B) qRT-PCR using ScNCE103 (top) and CST6 (bottom) specific primers and RNA extracted from the S. cerevisiae controls, ScNCE103 mutant and CST6 mutant strain grown in air enriched with 5.5% CO2 (top panel) or air (bottom panel). Both mutants show no significant level of ScNCE103 and CST6 transcript compared to the control strain. C) qRT-PCR using ORF19.6103 (top) and MVD (bottom) specific primers and RNA extracted from C. albicans control and the RCA1 mutant grown in air (black columns) or air enriched with 5.5% CO2 (white columns). Expression of both genes is not significantly different between the control and mutant strain. Data are represented as mean +/− SD from three independent experiments. Asterisk indicates statistical significance determined by two-sample t test (P≤0.05).
Protein alignment of Rca1p and Cst6p sequences. Alignment of C. albicans Rca1p (C.a.) and S. cerevisiae Cst6p (S.c.) sequences by ClustalW2 (http://www.ebi.ac.uk/Tools/clustalw2/index.html). “*”, “:” and “.” respectively means that the residues of that column are identical in the two sequences, that conserved substitutions occurred, or that semi-conserved substitutions are observed. The bZIP motifs (bold) are present in the C-terminus of each protein. 3 conserved putative serine sites for phosphorylation (underlined) are shown (http://www.cbs.dtu.dk/services/NetPhosYeast/).
The RCA1 heterozygous mutant and complemented strains display a wild-type pattern of Nce103p expression. Carbonic anhydrase signals are shown in western blots from the C. albicans control, RCA1 heterozygous mutant, and the complemented strains. All strains display an identical profile of Nce103p expression. The same samples were probed with an anti-actin antibody as control.
RCA1 inactivation does not impact on cells morphology. Control (CAI4+pSM2) and rca1 mutant (rca1Δ) strain were grown for 2h at 37°C in YPD supplemented or not with 5% horse serum. Representative pictures show identical morphology for both strains in YPD and confirm the inability of the rca1 mutant to differentiate into hyphae. Bar corresponds to 5 µm.
C.albicans Nce103p is not regulated by environmental pH. Western blots showing carbonic anhydrase signals from the C. albicans control strain grown in YPD buffered at pH 4 or pH 7 for 4h in air. In both conditions, Nce103p signals are identical.
Nce103p induction is exquisitely sensitive to ambient CO2 availability. Using appropriate antibodies, carbonic anhydrase signals are shown in western blots from S. cerevisiae (top) and C. albicans (bottom). Proteins were extracted from cells grown in YPD for 4h in air or air enriched with 0.5 or 1% CO2. Nce103p signals are detectable in ambient air, and air enriched with 0.5% CO2.
Detailed protocols about media used in this study, strains and plasmids construction, yeast transformations, Southern blot analysis and the generation of C. albicans Nce103p antibodies, as well as supporting references.
Rca1p-HA3 binding in air dataset. The following criteria were used: Log2 pseudo-median signal intensity threshold of ≥0.5 and p-value cut-off of ≤0.01 . Contig19#: The Contig19 number on which a given binding peak is detected using the Tilescope software . Location: Position of the binding peak in the corresponding Contig19 DNA sequence. Log2 pseudo-median signal intensity: Log2-transformed pseudo-median signal intensity of Rca1p-HA3-binding at the corresponding location. Target: orf19 nomenclature according to the C. albicans Assembly 19 of Rca1p-HA3 target gene, based on the location of the locus relative to the binding peak. Absence of information indicates that binding peaks are not clearly associated with promoter of ORFs. If the peak was found in the promoter region common to two adjacent ORFs, the two possible predicted target genes are shown, separated by “and”. CGD Gene name: Gene name of the corresponding target gene according to the Candida Genome Database (CGD) (www.candidagenome.org). Description: Gene description according to CGD.
Rca1p-HA3 binding in 5.5% CO2 dataset. The following criteria were used: Log2 pseudo-median signal intensity threshold of ≥0.5 and p-value cut-off of ≤0.01 . Contig19#: The Contig19 number on which a given binding peak is detected using the Tilescope software . Location: Position of the binding peak in the corresponding Contig19 DNA sequence. Log2 pseudo-median signal intensity: Log2-transformed pseudo-median signal intensity of Rca1p-HA3-binding at the corresponding location. Target: orf19 nomenclature according to the C. albicans Assembly 19 of Rca1p-HA3 target gene, based on the location of the locus relative to the binding peak. Absence of information indicates that binding peaks are not clearly associated with promoter of ORFs. If the peak was found in the promoter region common to two adjacent ORFs, the two possible predicted target genes are shown, separated by “and”. CGD Gene name: Gene name of the corresponding target gene according to the Candida Genome Database (CGD) (www.candidagenome.org). Description: Gene description according to CGD.
Plasmids used and construct in this study.
Strains used and constructed in this study.
We would like to thank Dr. C. Gourlay and Dr. T. von der Haar for their fruitful discussion and their help with S. cerevisiae experiments and Dr. Y. Wang for access to equipment. We thank Laura Kemp, Marianna Murphy, Hana Ždarská and Sandra Weber for their technical assistance.
We are grateful to the School of Biosciences, University of Kent for continuous support.
Conceived and designed the experiments: FC MCL ZP FAM. Performed the experiments: FC MB WL CJL LV ZP. Analyzed the data: FC MCL MR OK ZP FAM. Contributed reagents/materials/analysis tools: MCL DS MR OK NP ZP. Wrote the paper: FC OK MCL FAM.
- 1. Gaugler CD, Lebeck L, Nakagaki B, Boush GM (1980) Orientation of the entomogenous nematode Neoaplectana carpocapsae to carbon dioxide. Environ Entomol 9: 649–652.CD GauglerL. LebeckB. NakagakiGM Boush1980Orientation of the entomogenous nematode Neoaplectana carpocapsae to carbon dioxide.Environ Entomol9649652
- 2. Bretscher AJ, Busch KE, de Bono M (2008) A carbon dioxide avoidance behavior is integrated with responses to ambient oxygen and food in Caenorhabditis elegans. Proc Natl Acad Sci U S A 105: 8044–8049.AJ BretscherKE BuschM. de Bono2008A carbon dioxide avoidance behavior is integrated with responses to ambient oxygen and food in Caenorhabditis elegans.Proc Natl Acad Sci U S A10580448049
- 3. Suh GS, Wong AM, Hergarden AC, Wang JW, Simon AF, et al. (2004) A single population of olfactory sensory neurons mediates an innate avoidance behaviour in Drosophila. Nature 431: 854–859.GS SuhAM WongAC HergardenJW WangAF Simon2004A single population of olfactory sensory neurons mediates an innate avoidance behaviour in Drosophila.Nature431854859
- 4. Jones WD, Cayirlioglu P, Kadow IG, Vosshall LB (2007) Two chemosensory receptors together mediate carbon dioxide detection in Drosophila. Nature 445: 86–90.WD JonesP. CayirliogluIG KadowLB Vosshall2007Two chemosensory receptors together mediate carbon dioxide detection in Drosophila.Nature4458690
- 5. Lindskog S (1997) Structure and mechanism of carbonic anhydrase. Pharmacol Ther 74: 1–20.S. Lindskog1997Structure and mechanism of carbonic anhydrase.Pharmacol Ther74120
- 6. Gotz R, Gnann A, Zimmermann FK (1999) Deletion of the carbonic anhydrase-like gene NCE103 of the yeast Saccharomyces cerevisiae causes an oxygen-sensitive growth defect. Yeast 15: 855–864.R. GotzA. GnannFK Zimmermann1999Deletion of the carbonic anhydrase-like gene NCE103 of the yeast Saccharomyces cerevisiae causes an oxygen-sensitive growth defect.Yeast15855864
- 7. Klengel T, Liang WJ, Chaloupka J, Ruoff C, Schroppel K, et al. (2005) Fungal adenylyl cyclase integrates CO2 sensing with cAMP signaling and virulence. Curr Biol 15: 2021–2026.T. KlengelWJ LiangJ. ChaloupkaC. RuoffK. Schroppel2005Fungal adenylyl cyclase integrates CO2 sensing with cAMP signaling and virulence.Curr Biol1520212026
- 8. Elleuche S, Poggeler S (2009) Beta-carbonic anhydrases play a role in fruiting body development and ascospore germination in the filamentous fungus Sordaria macrospora. PLoS One 4: e5177.S. ElleucheS. Poggeler2009Beta-carbonic anhydrases play a role in fruiting body development and ascospore germination in the filamentous fungus Sordaria macrospora.PLoS One4e5177
- 9. Han KH, Chun YH, Figueiredo Bde C, Soriani FM, Savoldi M, et al. (2010) The conserved and divergent roles of carbonic anhydrases in the filamentous fungi Aspergillus fumigatus and Aspergillus nidulans. Mol Microbiol 75: 1372–1388.KH HanYH ChunC. Figueiredo BdeFM SorianiM. Savoldi2010The conserved and divergent roles of carbonic anhydrases in the filamentous fungi Aspergillus fumigatus and Aspergillus nidulans.Mol Microbiol7513721388
- 10. Bahn YS, Cox GM, Perfect JR, Heitman J (2005) Carbonic anhydrase and CO2 sensing during Cryptococcus neoformans growth, differentiation, and virulence. Curr Biol 15: 2013–2020.YS BahnGM CoxJR PerfectJ. Heitman2005Carbonic anhydrase and CO2 sensing during Cryptococcus neoformans growth, differentiation, and virulence.Curr Biol1520132020
- 11. Huang G, Srikantha T, Sahni N, Yi S, Soll DR (2009) CO(2) regulates white-to-opaque switching in Candida albicans. Curr Biol 19: 330–334.G. HuangT. SrikanthaN. SahniS. YiDR Soll2009CO(2) regulates white-to-opaque switching in Candida albicans.Curr Biol19330334
- 12. Mogensen EG, Janbon G, Chaloupka J, Steegborn C, Fu MS, et al. (2006) Cryptococcus neoformans senses CO2 through the carbonic anhydrase Can2 and the adenylyl cyclase Cac1. Eukaryot Cell 5: 103–111.EG MogensenG. JanbonJ. ChaloupkaC. SteegbornMS Fu2006Cryptococcus neoformans senses CO2 through the carbonic anhydrase Can2 and the adenylyl cyclase Cac1.Eukaryot Cell5103111
- 13. Hall RA, De Sordi L, Maccallum DM, Topal H, Eaton R, et al. (2010) CO(2) acts as a signalling molecule in populations of the fungal pathogen Candida albicans. PLoS Pathog 6: e1001193.RA HallL. De SordiDM MaccallumH. TopalR. Eaton2010CO(2) acts as a signalling molecule in populations of the fungal pathogen Candida albicans.PLoS Pathog6e1001193
- 14. Neri D, Supuran CT (2011) Interfering with pH regulation in tumours as a therapeutic strategy. Nat Rev Drug Discov 10: 767–77.D. NeriCT Supuran2011Interfering with pH regulation in tumours as a therapeutic strategy.Nat Rev Drug Discov1076777
- 15. Wykoff CC, Beasley NJ, Watson PH, Turner KJ, Pastorek J, et al. (2000) Hypoxia-inducible expression of tumor-associated carbonic anhydrases. Cancer Res 60: 7075–7083.CC WykoffNJ BeasleyPH WatsonKJ TurnerJ. Pastorek2000Hypoxia-inducible expression of tumor-associated carbonic anhydrases.Cancer Res6070757083
- 16. Kovacikova G, Lin W, Skorupski K (2010) The LysR-type virulence activator AphB regulates the expression of genes in Vibrio cholerae in response to low pH and anaerobiosis. J Bacteriol 192: 4181–4191.G. KovacikovaW. LinK. Skorupski2010The LysR-type virulence activator AphB regulates the expression of genes in Vibrio cholerae in response to low pH and anaerobiosis.J Bacteriol19241814191
- 17. Aguilera J, Petit T, de Winde JH, Pronk JT (2005) Physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to high carbon dioxide concentrations. FEMS Yeast Res 5: 579–593.J. AguileraT. PetitJH de WindeJT Pronk2005Physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to high carbon dioxide concentrations.FEMS Yeast Res5579593
- 18. Amoroso G, Morell-Avrahov L, Muller D, Klug K, Sultemeyer D (2005) The gene NCE103 (YNL036w) from Saccharomyces cerevisiae encodes a functional carbonic anhydrase and its transcription is regulated by the concentration of inorganic carbon in the medium. Mol Microbiol 56: 549–558.G. AmorosoL. Morell-AvrahovD. MullerK. KlugD. Sultemeyer2005The gene NCE103 (YNL036w) from Saccharomyces cerevisiae encodes a functional carbonic anhydrase and its transcription is regulated by the concentration of inorganic carbon in the medium.Mol Microbiol56549558
- 19. Rocha CR, Schroppel K, Harcus D, Marcil A, Dignard D, et al. (2001) Signaling through adenylyl cyclase is essential for hyphal growth and virulence in the pathogenic fungus Candida albicans. Mol Biol Cell 12: 3631–3643.CR RochaK. SchroppelD. HarcusA. MarcilD. Dignard2001Signaling through adenylyl cyclase is essential for hyphal growth and virulence in the pathogenic fungus Candida albicans.Mol Biol Cell1236313643
- 20. Fonzi WA, Irwin MY (1993) Isogenic strain construction and gene mapping in Candida albicans. Genetics 134: 717–728.WA FonziMY Irwin1993Isogenic strain construction and gene mapping in Candida albicans.Genetics134717728
- 21. Lorenz MC, Bender JA, Fink GR (2004) Transcriptional response of Candida albicans upon internalization by macrophages. Eukaryot Cell 3: 1076–1087.MC LorenzJA BenderGR Fink2004Transcriptional response of Candida albicans upon internalization by macrophages.Eukaryot Cell310761087
- 22. Lorenz MC, Fink GR (2001) The glyoxylate cycle is required for fungal virulence. Nature 412: 83–86.MC LorenzGR Fink2001The glyoxylate cycle is required for fungal virulence.Nature4128386
- 23. Garcia-Gimeno MA, Struhl K (2000) Aca1 and Aca2, ATF/CREB activators in Saccharomyces cerevisiae, are important for carbon source utilization but not the response to stress. Mol Cell Biol 20: 4340–4349.MA Garcia-GimenoK. Struhl2000Aca1 and Aca2, ATF/CREB activators in Saccharomyces cerevisiae, are important for carbon source utilization but not the response to stress.Mol Cell Biol2043404349
- 24. Srikantha T, Borneman AR, Daniels KJ, Pujol C, Wu W, et al. (2006) TOS9 regulates white-opaque switching in Candida albicans. Eukaryot Cell 5: 1674–1687.T. SrikanthaAR BornemanKJ DanielsC. PujolW. Wu2006TOS9 regulates white-opaque switching in Candida albicans.Eukaryot Cell516741687
- 25. Znaidi S, Barker KS, Weber S, Alarco AM, Liu TT, et al. (2009) Identification of the Candida albicans Cap1p regulon. Eukaryot Cell 8: 806–820.S. ZnaidiKS BarkerS. WeberAM AlarcoTT Liu2009Identification of the Candida albicans Cap1p regulon.Eukaryot Cell8806820
- 26. Bates S, Hughes HB, Munro CA, Thomas WP, MacCallum DM, et al. (2006) Outer chain N-glycans are required for cell wall integrity and virulence of Candida albicans. J Biol Chem 281: 90–98.S. BatesHB HughesCA MunroWP ThomasDM MacCallum2006Outer chain N-glycans are required for cell wall integrity and virulence of Candida albicans.J Biol Chem2819098
- 27. Elson SL, Noble SM, Solis NV, Filler SG, Johnson AD (2009) An RNA transport system in Candida albicans regulates hyphal morphology and invasive growth. PLoS Genet 5: e1000664.SL ElsonSM NobleNV SolisSG FillerAD Johnson2009An RNA transport system in Candida albicans regulates hyphal morphology and invasive growth.PLoS Genet5e1000664
- 28. Vachova L, Chernyavskiy O, Strachotova D, Bianchini P, Burdikova Z, et al. (2009) Architecture of developing multicellular yeast colony: spatio-temporal expression of Ato1p ammonium exporter. Environ Microbiol 11: 1866–77.L. VachovaO. ChernyavskiyD. StrachotovaP. BianchiniZ. Burdikova2009Architecture of developing multicellular yeast colony: spatio-temporal expression of Ato1p ammonium exporter.Environ Microbiol11186677
- 29. Schmid A, Sutto Z, Schmid N, Novak L, Ivonnet P, et al. (2010) Decreased soluble adenylyl cyclase activity in cystic fibrosis is related to defective apical bicarbonate exchange and affects ciliary beat frequency regulation. J Biol Chem 285: 29998–30007.A. SchmidZ. SuttoN. SchmidL. NovakP. Ivonnet2010Decreased soluble adenylyl cyclase activity in cystic fibrosis is related to defective apical bicarbonate exchange and affects ciliary beat frequency regulation.J Biol Chem2852999830007
- 30. Supuran CT (2008) Carbonic anhydrases: novel therapeutic applications for inhibitors and activators. Nat Rev Drug Discov 7: 168–181.CT Supuran2008Carbonic anhydrases: novel therapeutic applications for inhibitors and activators.Nat Rev Drug Discov7168181
- 31. Schlicker C, Hall RA, Vullo D, Middelhaufe S, Gertz M, et al. (2009) Structure and inhibition of the CO2-sensing carbonic anhydrase Can2 from the pathogenic fungus Cryptococcus neoformans. J Mol Biol 385: 1207–1220.C. SchlickerRA HallD. VulloS. MiddelhaufeM. Gertz2009Structure and inhibition of the CO2-sensing carbonic anhydrase Can2 from the pathogenic fungus Cryptococcus neoformans.J Mol Biol38512071220
- 32. Schofield CJ, Ratcliffe PJ (2005) Signalling hypoxia by HIF hydroxylases. Biochem Biophys Res Commun 338: 617–626.CJ SchofieldPJ Ratcliffe2005Signalling hypoxia by HIF hydroxylases.Biochem Biophys Res Commun338617626
- 33. Parks SK, Chiche J, Pouyssegur J (2011) pH control mechanisms of tumor survival and growth. J Cell Physiol 226: 299–308.SK ParksJ. ChicheJ. Pouyssegur2011pH control mechanisms of tumor survival and growth.J Cell Physiol226299308
- 34. Setiadi ER, Doedt T, Cottier F, Noffz C, Ernst JF (2006) Transcriptional response of Candida albicans to hypoxia: linkage of oxygen sensing and Efg1p-regulatory networks. J Mol Biol 361: 399–411.ER SetiadiT. DoedtF. CottierC. NoffzJF Ernst2006Transcriptional response of Candida albicans to hypoxia: linkage of oxygen sensing and Efg1p-regulatory networks.J Mol Biol361399411
- 35. Carlson JM, Chakravarty A, DeZiel CE, Gross RH (2007) SCOPE: a web server for practical de novo motif discovery. Nucleic Acids Res 35: W259–264.JM CarlsonA. ChakravartyCE DeZielRH Gross2007SCOPE: a web server for practical de novo motif discovery.Nucleic Acids Res35W259264
- 36. Taylor JW, Berbee ML (2006) Dating divergences in the Fungal Tree of Life: review and new analyses. Mycologia 98: 838–849.JW TaylorML Berbee2006Dating divergences in the Fungal Tree of Life: review and new analyses.Mycologia98838849
- 37. Lavoie H, Hogues H, Mallick J, Sellam A, Nantel A (2010) Evolutionary tinkering with conserved components of a transcriptional regulatory network. PLoS Biol 8: e1000329.H. LavoieH. HoguesJ. MallickA. SellamA. Nantel2010Evolutionary tinkering with conserved components of a transcriptional regulatory network.PLoS Biol8e1000329
- 38. Kaluz S, Kaluzova M, Liao SY, Lerman M, Stanbridge EJ (2009) Transcriptional control of the tumor- and hypoxia-marker carbonic anhydrase 9: A one transcription factor (HIF-1) show? Biochim Biophys Acta 1795: 162–172.S. KaluzM. KaluzovaSY LiaoM. LermanEJ Stanbridge2009Transcriptional control of the tumor- and hypoxia-marker carbonic anhydrase 9: A one transcription factor (HIF-1) show?Biochim Biophys Acta1795162172
- 39. Brennan DJ, Jirstrom K, Kronblad A, Millikan RC, Landberg G, et al. (2006) CA IX is an independent prognostic marker in premenopausal breast cancer patients with one to three positive lymph nodes and a putative marker of radiation resistance. Clin Cancer Res 12: 6421–6431.DJ BrennanK. JirstromA. KronbladRC MillikanG. Landberg2006CA IX is an independent prognostic marker in premenopausal breast cancer patients with one to three positive lymph nodes and a putative marker of radiation resistance.Clin Cancer Res1264216431
- 40. Demirci A, Pometto AL 3rd, Ho KL (1997) Ethanol production by Saccharomyces cerevisiae in biofilm reactors. J Ind Microbiol Biotechnol 19: 299–304.A. DemirciAL Pometto 3rdKL Ho1997Ethanol production by Saccharomyces cerevisiae in biofilm reactors.J Ind Microbiol Biotechnol19299304
- 41. Zara S, Gross MK, Zara G, Budroni M, Bakalinsky AT (2010) Ethanol-independent biofilm formation by a flor wine yeast strain of Saccharomyces cerevisiae. Appl Environ Microbiol 76: 4089–4091.S. ZaraMK GrossG. ZaraM. BudroniAT Bakalinsky2010Ethanol-independent biofilm formation by a flor wine yeast strain of Saccharomyces cerevisiae.Appl Environ Microbiol7640894091
- 42. Huang G, Yi S, Sahni N, Daniels KJ, Srikantha T, et al. (2010) N-acetylglucosamine induces white to opaque switching, a mating prerequisite in Candida albicans. PLoS Pathog 6: e1000806.G. HuangS. YiN. SahniKJ DanielsT. Srikantha2010N-acetylglucosamine induces white to opaque switching, a mating prerequisite in Candida albicans.PLoS Pathog6e1000806
- 43. Liu TT, Znaidi S, Barker KS, Xu L, Homayouni R, et al. (2007) Genome-wide expression and location analyses of the Candida albicans Tac1p regulon. Eukaryot Cell 6: 2122–2138.TT LiuS. ZnaidiKS BarkerL. XuR. Homayouni2007Genome-wide expression and location analyses of the Candida albicans Tac1p regulon.Eukaryot Cell621222138
- 44. Zhang ZD, Rozowsky J, Lam HY, Du J, Snyder M, et al. (2007) Tilescope: online analysis pipeline for high-density tiling microarray data. Genome Biol 8: R81.ZD ZhangJ. RozowskyHY LamJ. DuM. Snyder2007Tilescope: online analysis pipeline for high-density tiling microarray data.Genome Biol8R81