Kaposi's Sarcoma (KS), the most common tumor of AIDS patients, is a highly vascularized tumor supporting large amounts of angiogenesis. The main cell type of KS tumors is the spindle cell, a cell of endothelial origin, the primary cell type involved in angiogenesis. Kaposi's Sarcoma-associated herpesvirus (KSHV) is the etiologic agent of KS and is likely involved in both tumor formation and the induction of angiogenesis. Integrins, and specifically integrin αVβ3, have known roles in both tumor induction and angiogenesis. αVβ3 is also important for KSHV infection as it has been shown to be involved in KSHV entry into cells. We found that during latent infection of endothelial cells KSHV induces the expression of integrin β3 leading to increased surface levels of αVβ3. Signaling molecules downstream of integrins, including FAK and Src, are activated during viral latency. Integrin activation by KSHV is necessary for the KSHV-associated upregulation of a number of angiogenic phenotypes during latent infection including adhesion and motility. Additionally, KSHV-infected cells become more reliant on αVβ3 for capillary like formation in three dimensional culture. KSHV induction of integrin β3, leading to induction of angiogenic and cancer cell phenotypes during latency, is likely to be important for KS tumor formation and potentially provides a novel target for treating KS tumors.
Kaposi's Sarcoma (KS) is the most common tumor of AIDS patients world-wide and is characterized by very high vascularization. The main KS tumor cell type is the spindle cell, a cell of endothelial origin. Kaposi's Sarcoma-associated herpesvirus (KSHV), the etiologic agent of KS, is found predominantly in the latent state in spindle cells. In this study we examined how KSHV alters endothelial cells to induce phenotypes common to angiogenesis and tumor formation. Integrins are cell surface adhesion and signaling proteins that can be involved in tumor growth and tumor angiogenesis. We found that KSHV infection of endothelial cells leads to increased expression of integrin β3, a molecule that, when paired with its cognate α subunit, αV, has been shown to be critical for tumor-associated angiogenesis. KSHV infection promotes angiogenic phenotypes in endothelial cells including adhesion, motility and capillary morphogenesis, and these phenotypes require expression and signaling through integrin β3. Therefore, KSHV induction of integrin beta3 and downstream signaling is required for the induction of phenotypes thought to be critical for KS tumor formation. αVβ3 inhibitors are in clinical trials for inhibition of tumors and we propose that these inhibitors may be clinically relevant for treatment of KS tumors.
Citation: DiMaio TA, Gutierrez KD, Lagunoff M (2011) Latent KSHV Infection of Endothelial Cells Induces Integrin Beta3 to Activate Angiogenic Phenotypes. PLoS Pathog 7(12): e1002424. https://doi.org/10.1371/journal.ppat.1002424
Editor: Blossom Damania, University of North Carolina at Chapel Hill, United States of America
Received: May 23, 2011; Accepted: October 21, 2011; Published: December 8, 2011
Copyright: © 2011 DiMaio et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: TD is supported by a post-doctoral fellowship from the American Cancer Society (119029-PF-10-166-01-MPC). KG is partially supported by an AIDS/STD training grant from the NIAID (T32A107140) and ML is supported by a grant from the NCI and from the NIDCR (RO1CA097934 and PO1DE021954). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Kaposi's sarcoma-associated herpesvirus (KSHV), a gamma herpesvirus, is the etiological agent for Kaposi's sarcoma (KS). KS is the most common tumor in AIDS patients world-wide, and is the most commonly reported tumor in parts of central Africa , . KS tumors are highly vascularized, with abnormal, leaky vasculature, and excess inflammation and edema. The histopathology of KS tumors supports a role for angiogenesis in tumor formation. The primary cell type of KS lesions are spindle-shaped endothelium-derived cells aptly named spindle cells. Nearly all spindle cells support latent KSHV infection, although a low percentage of cells undergoing lytic reactivation are always present .
KSHV can infect many types of cells in culture including endothelial cells , . KSHV infection of endothelial cells in culture leads to predominantly latent infection with a similar low percentage of cells undergoing lytic replication as in the KS tumor , . KSHV infection of endothelial cells can promote angiogenesis related phenotypes, including increased stability of tubules formed by macrovascular endothelial cells, induction of angiogenesis and capillary morphogenesis in low growth factor conditions, and enhanced migration and invasion –. Furthermore, KSHV infection can induce increased expression and secretion of signaling factors involved in angiogenesis, such as vascular endothelial growth factor (VEGF). Both VEGF-A and –C are expressed by KSHV-infected endothelial cells , . Interestingly, KSHV infection promotes the upregulation of both VEGF receptor 1, a blood vasculature marker, and VEGF receptor 3, a marker for lymphatic endothelium –. The upregulation of both VEGF receptors suggests KSHV-infected cells are more sensitive to the growth and migratory effects of VEGF than the surrounding uninfected endothelium. KSHV infection also leads to upregulation of other molecules with important roles in the regulation of angiogenesis. KSHV-induced expression of cyclooxygenase-2 (COX-2) as well as angiogenin was shown to be important for the maintenance of latency, as well as inflammatory cytokine expression and capillary morphogenesis . KSHV infection of endothelial cells upregulates several members of the angiopoietin family of growth factors, including angiopoietin-2 and angiopoietin-like 4, which are involved in regulating angiogenic remodeling and vessel stabilization -. In addition to secretion of growth factors, KSHV infection promotes disruption of adherens junctions, allowing for increased vascular permeability and invasion –. Furthermore, there have been several studies examining the role of other molecules and signaling pathways which have been implicated in KSHV-induced angiogenesis –.
Several KSHV genes have been shown to regulate expression of genes involved in angiogenesis. For example, both vIRF3 and the glycoprotein K1 promote VEGF expression , . Other genes were found to have angiogenic chemoattractant properties, such as the viral homologs to macrophage inflammatory proteins (vMIPs I–III; , ). The viral G-protein coupled receptor (vGPCR) is a constitutively active signaling receptor that has been linked to a variety of angiogenic signaling pathways , –. However, vGPCR and the other genes mentioned are primarily expressed during lytic replication and they have not been shown to be necessary for the induction of angiogenic phenotypes in the context of viral infection. Since only a small percentage of the infected cells undergo lytic replication, it is unknown if these proteins are sufficient to promote an angiogenic phenotype in the more abundant latently infected endothelial cells.
During latency in endothelial cells, only five viral genes are expressed, including the latency-associated nuclear antigen (LANA-1). LANA-1 has been shown to play a role in a host of processes including maintenance of the viral genome and host cell survival (reviewed in ). In addition to these functions, LANA-1 may play several roles in promoting angiogenesis. Expression of LANA-1 in endothelial cells induces upregulation of angiogenin, which may aid in induction of angiogenesis by VEGF and basic fibroblast growth factor . Furthermore, LANA-1 interaction with Daxx reduces its repression on Ets-1-dependent VEGF receptor expression . While it is apparent that KSHV genes are capable of inducing angiogenic pathways, a better understanding of how viral infection alters the host cell to induce angiogenesis is still needed.
Angiogenesis is a complex process that is tightly regulated by a delicate balance of pro- and anti-angiogenic factors. However, many pathogenic processes, such as tumor formation, shift this balance to promote continual vascular growth. How the different angiogenic signaling factors interact and are regulated is only partly understood. Along with secreted cytokines and growth factors, other signaling proteins such as integrins have been shown to regulate endothelial cell activation and angiogenesis. Integrins are cell surface proteins that link the extracellular matrix to the cytoskeleton. They form dimers of α and β subunits that recognize extracellular matrix (ECM) proteins and, upon ligand binding, undergo conformational changes and recruit intracellular adaptor and signaling molecules. Integrins can induce activation of focal adhesion kinase (FAK) leading to activation of Src kinase. Integrin signaling complexes play a role in a number of cellular processes such as adhesion to the ECM, migration, and cell survival during suspension, all of which are essential functions for endothelial cells during the process of angiogenesis (reviewed in –). In particular, αVβ3 integrin has been shown to be upregulated in the vasculature associated with a number of tumor types and has been linked to regulation of angiogenesis –. Antagonists of integrin αVβ3 inhibit tumor angiogenesis and tumor growth in a variety of animal models of cancer –. Interestingly, αVβ3 integrin is a receptor for several viruses, including KSHV . KSHV glycoprotein B (gB) can associate with integrins on the cell surface leading to increased signaling, adhesion, and to cytoskeletal rearrangements. However, previous studies have not examined the role of integrins during latent KSHV infection.
We have identified a role for integrin β3 during latent infection of KSHV in endothelial cells. Infected cells upregulate integrin β3 expression leading to increased cell surface αVβ3. Latently infected endothelial cells become more adherent to integrin ligands fibronectin and vitronectin, and are also more migratory than mock-infected cells. These induced phenotypes require RGD-binding integrins, specifically integrin β3. Interestingly, although both uninfected and infected cells organize in three-dimensional culture, infected cells are more sensitive to inhibitors of integrin β3 and its downstream signaling molecules, such as Src kinase. This suggests that during latent KSHV infection there is a shift in endothelial cell signaling that results in a more angiogenic phenotype dependent on αVβ3.
Latent KSHV infection upregulates integrin β3 expression
Our microarray analysis of mock- and KSHV-infected endothelial cells indicated that integrin β3 was significantly upregulated in all our KSHV-infected samples (data not shown). To confirm this we utilized quantitative real-time RT-PCR to measure integrin β3 mRNA levels during KSHV latent infection of endothelial cells. TIME or primary human dermal microvascular endothelial cells were mock- or KSHV-infected and allowed to establish latent infection for 48 hours. RNA from these cells was extracted and subjected to real-time RT-PCR using primers specific to integrin β3. Figure 1A shows that KSHV-infected TIME cells had a 4.6-fold increase in integrin β3 expression as compared to their mock counterparts. In primary endothelial cells (ECs), KSHV induced a 3.5-fold increase in integrin β3 expression over mock infection. In the cultures analyzed, greater than 90% of the infected cells expressed LANA and less than 1% of the cells expressed ORF 59, a marker of lytic infection, indicating that the increase is likely to be a result of latent infection.
A) TIME cells or primary human dermal microvascular cells were either mock- or KSHV-infected, and allowed to establish latency for 48 hours. Relative abundances of integrin β3 mRNA were analyzed by one-step real-time RT-PCR. B) 48 hours post-infection mock- or KSHV-infected TIME cells were harvested and cell lysates were separated by SDS-PAGE and stained with an antibody to integrin β3. C) Mock- (red line) or KSHV-infected (blue line) TIME cells were stained with antibody to mouse IGG or αVβ3 and analyzed by flow cytometry.
We next examined the expression of integrin β3 at the protein level, using western blot analysis and flow cytometry. In TIME cells latently infected with KSHV, total cellular integrin β3 was significantly increased as visualized by Western blot analysis with an antibody specific to integrin β3 (Figure 1B). In endothelial cells αV is the only integrin α subunit that dimerizes with the β3 subunit. Flow cytometric analysis showed a specific increase in cell surface expression of αVβ3 during latent KSHV infection of TIME cells (Figure 1C). The mean fluorescence intensity of mock-infected cells was approximately 22.3, while KSHV infection increased the mean to 31.9. Although there is only a modest increase in cell surface αVβ3 protein expression as compared to total cellular expression, this shift was highly reproducible upon multiple infections with different stocks of virus. Importantly, expression of αVβ3 increased in the majority of cells, indicating that the increased surface protein expression was a property of latently infected cells, not only the low percentage of lytically infected cells.
Integrin signaling through FAK and Src
Activation of integrins leads to recruitment of the intracellular protein focal adhesion kinase (FAK) and autophosphorylation of FAK on tyrosine 397 . This autophosphorylation provides a binding site for src family kinases, which subsequently become activated. To determine if KSHV promotes integrin signaling through FAK and Src, we analyzed the phosphorylation state of these proteins at 48 hours post infection, after the establishment of latency. Figure 2A shows an increase in FAK phosphorylation on tyrosine 397 in KSHV-infected cells, compared to mock-infected cells. Furthermore, Src is also phosphorylated more heavily on tyrosine 416 in KSHV-infected cells. These experiments were also performed in primary HMVECs, which upon infection undergo a small but reproducible increase in Fak and Src activation (Figure 2B). These data suggest that KSHV latent infection promotes signaling through FAK and Src.
TIME cells (A) or primary dHMVECs (B) were mock- or KSHV-infected. At 48 hpi, cell extracts were analyzed by immunoblot analysis with the indicated phospho-specific or total-protein antibodies. Blots were stripped and probed with an antibody to β-actin as a loading control. The numbers below the lanes indicate the relative abundance of each major band. These experiments were repeated three times with similar results.
KSHV promotes focal adhesion complex turnover
A key process during cell migration is the regulation of focal adhesion complexes, groups of signaling molecules that mediate integrin signaling to the actin cytoskeleton. In order to better understand the effect of KSHV induction of integrin β3 on endothelial cell morphology, we examined the formation of focal adhesion complexes in mock- or KSHV-infected cells. Figure 3A shows the localization of the focal adhesion component vinculin in mock-infected cells, which have many centrally localized focal adhesions. The cells were also stained with phalloidin to identify polymerized actin which is present throughout the cells. In contrast, at 48 hours post-infection, endothelial cells latently infected with KSHV have fewer focal adhesions but they are strongly localized to the periphery, suggesting changes in the formation and turnover of the focal adhesions (Figure 3C). The polymerized actin also localizes to the periphery of the cell during latent infection. The fewer focal adhesions and their localization at the periphery suggest higher turnover and increased migration in KSHV-infected cells.
Primary HMVECs were infected with mock (A,D,G), UV-inactivated KSHV (B,E,H), or KSHV (C,F,I) and allowed to establish latency for 48 hours. A–C) Cells were then fixed and stained for vinculin (red) and phalloidin to visualize polymerized actin (green). Cells were mounted in medium containing DAPI (blue). Arrows indicate focal adhesions. Images were taken at 630x magnification. D–F) Cells were stained with antibody to integrin β3 (red) and phalloidin (green). Arrows indicate integrin β3 localized to focal adhesions. Images were taken at 630x magnification. G–I) Cells were stained with antibody to LANA (red) and phallodin (green) as above. Images were taken at 400x magnification. I) Arrows show LANA-positive KSHV-infected cells, while arrow heads point to LANA-negative uninfected cells in the same culture.
To eliminate the potential effects of virus binding and entry, UV-inactivated virus was used to infect cells. UV-irradiated virus can bind and enter cells but there is no viral gene expression, as determined by immunofluorescence with anti-LANA antibodies. UV-inactivated virus did not alter the localization of focal adhesions at 48 hours post-infection (Figure 3B). Interestingly, in a mixed population of infected and uninfected cells, only infected cells (as determined by staining with LANA-1 antibody) had peripheral localization of focal adhesions. Uninfected cells in the same population had more centrally localized focal adhesions, similar to mock-infected cells (data not shown).
The cells were similarly stained with phalloidin and antibodies to integrin β3. Integrins are known components of focal adhesion complexes and have previously been shown to localize with vinculin in these complexes , , . As expected, integrin β3 had a very similar pattern of relocalization to the periphery as was seen with antibodies to vinculin (Figure 3D–F). These data suggest that latent KSHV infection promotes formation and turnover of focal adhesions and that integrin αVβ3 is activated and localized to the newly formed focal adhesions. These observations are visible throughout the culture, as can be seen in Figure 3G–I, where multiple cells are shown stained for the latent antigen LANA (red) and the actin cytoskeleton (green). Latently infected cells (Figure 3I, arrows) show peripheral organization of actin filaments, compared to the neighboring uninfected cells in the same culture (Figure 3I, arrow heads), which have actin organization similar to mock-infected or UV-inactivated virus (Figure 3G–H). Furthermore, peripheral focal adhesions can be detected in greater than 90% of the highly infected cells. In contrast, ORF59 is detected in less than 1% of the cells (data not shown). In our endothelial infections the KSHV late lytic protein K8.1, is detected in an even lower percentage of the cells than ORF59 and we could only detect gB in a very low percentage of cells as well (data not shown). Importantly, gB has previously been shown to be detected only in the low percentage of cells undergoing lytic replication . Together, these data indicate that the vast majority of cells with peripheral focal adhesions are latently infected and, while lytic replication could play some paracrine role, it is unlikely to play a primary role in organization of focal adhesion complexes during KSHV infection as uninfected cells would be altered as well in this scenario.
KSHV promotes endothelial cell adhesion and migration in an RGD- and integrin β3-dependent fashion
We next wanted to determine whether KSHV latent infection promotes an angiogenic phenotype in endothelial cells through the activation of αVβ3. Adhesion and migration of endothelial cells are both critical for angiogenesis. Figure 4 shows the adhesion of mock- or KSHV-infected cells to various concentrations of the extracellular matrix proteins fibronectin and vitronectin, both of which contain the arginine-glycine-aspartic acid (RGD) motif that is recognized by a subset of integrins, including αVβ3. Latent infection by KSHV enhanced the adhesion of endothelial cells to both fibronectin (Figure 4A) and vitronectin (Figure 4B) compared to mock-infected cells. None of the cells adhered to either collagen I, which lacks an RGD motif, or laminin, suggesting the increased adhesion is specific to fibronectin and vitronectin (data not shown). Short RGD-containing peptides will compete with vitronectin and fibronectin binding to RGD-dependent integrins, like αVβ3, and inhibit integrin-dependent adherence to these substrates. Interestingly, an RGD-containing short peptide, but not a control RAD- or RGE-containing peptide, inhibited KSHV-induced adhesion to fibronectin or vitronectin. The same peptides had little effect on mock-infected cells (Figure 4C–D). This indicates that KSHV-infected cells require RGD-binding integrins for increased adherence while mock-infected cells are relatively unaffected by the concentrations of RGD peptides used.
TIME cells were mock- or KSHV-infected and 48 hours post-infection cells were plated onto 96-well plates coated with fibronectin (A) or vitronectin (B). Cells were allowed to adhere for 90 minutes after which non-adherent cells were washed off. The number of adherent cells was determined by alkaline phosphatase activity. Mock-infected cell adhesion is indicated with squares and KSHV-infected cell adhesion is indicated with triangles. C and D) 48 hours post-infection, mock- (squares) and KSHV- (triangles) infected cells were pretreated with RGD-containing (red lines) or control peptide (RAD for (C) and RGE for (D), black lines) for 15 minutes on ice before assaying for adhesion to fibronectin (C) or vitronectin (D).
In addition to adhesion, integrin activation can promote migration of endothelial cells through formation and turnover of focal adhesions. In Figure 3 we demonstrated a strong reorganization of focal adhesions during KSHV latency in endothelial cells. We therefore examined the effect of KSHV infection on endothelial cell migration. 48 hours post-infection, endothelial cells were seeded on a transwell membrane with 8 µm pore size and allowed to migrate for 1.5 hours. Approximately three times more KSHV-infected endothelial cells migrated through the pores as compared to mock-infected cells (Figure 5A–C). This increase in migration was not due to increased proliferation, since KSHV infection does not promote proliferation in TIME cells as determined by uptake of BrdU (data not shown). To determine whether this enhanced migration was due to increased integrin signaling, we used a Src kinase inhibitor to block downstream integrin signaling. The Src kinase inhibitor led to a decrease in the mean number of mock- and KSHV-infected cells that migrated through the pores (Figure 5D). However, the lower dose of the Src kinase inhibitor decreased the migration of mock-infected cells by approximately 50% while migration of KSHV-infected cells was reduced more than 80% (Figure 5E). Therefore, KSHV-infected cell migration is sensitive to Src inhibitors to a greater degree than migration of mock-infected cells.
Cells were infected with mock or KSHV and, 48 hours post-infection, were plated on fibronectin-coated transwells. Cells were allowed to migrate through the pores at 37°C for 1.5 hours and subsequently fixed and stained with crystal violet. The cells on the upper side of the membrane were removed with a cotton swab, the membranes were mounted on slides and the cells that migrated were photographed. A and B) Representative images of migrated mock- (A) and KSHV-infected (B) cells. C) Quantification of a representative experiment showing the number of migrated cells per field for 10 fields counted per transwell. Asterisk indicates a p-value of less than 0.05 compared to mock-infected cells. D–E) Cells (48 hpi) were treated with the Src kinase inhibitor SKI-1 at the time of plating on fibronectin-coated transwells and are presented as number of cells per field (D) and percent inhibition by SKI-1 (E). Double asterisks indicate a p-value of less than 0.005 compared to DMSO control.
Integrin β3 activation is required for KSHV-induced capillary morphogenesis
KSHV latent infection leads to small increases in the ability of endothelial cells to organize into capillary-like structures in three-dimensional culture at 6 hours after seeding. However, these effects are not consistently statistically significant (our unpublished observations and Figure 6A). Due to the increased integrin activity and signaling, we hypothesized that KSHV infection may promote capillary morphogenesis through a different pathway than uninfected cells. Therefore, we examined the role of integrin β3 in the ability of KSHV-infected cells to organize in three-dimensional culture on Matrigel. We first tested whether RGD-binding integrins were involved by using an RGD-containing peptide to block integrin activity. Interestingly, in the presence of the RGD-containing peptide, KSHV-infected cells formed approximately 85% fewer capillary-like structures while mock-infected cells lost only 40% of their activity (Figure 6A and B). This indicates that KSHV-infected cells are more sensitive to inhibition of integrin activation as compared to mock-infected cells and suggests that KSHV-infected cells become reliant on integrin activity for this angiogenic phenotype.
A)TIME cells were mock- or KSHV-infected and, 48 hpi, plated on Matrigel in the presence of either RAD- or RGD-containing peptide in complete medium. B) The data in the graphs are the mean number of capillaries per field (left) and the percent of RAD peptide controls (right). C–D) TIME cells were mock- (C) or KSHV-infected (D), and, at 24 hpi, transfected with either control siRNA or siRNA specific to integrin β3. Cells were stained with antibody to mouse IGG (red line) or αVβ3 integrin (blue line: control siRNA; green line: integrin β3 siRNA) and analyzed by flow cytometry. E) At 72 hours post infection and 48 hours post transfection of integrin β3 siRNA, mock- and KSHV-infected cells were plated on Matrigel in complete media and cultured for 6 hours. F) Quantitation of three separate experiments similar to the one shown in E. G) TIME cells were mock- or KSHV-infected and, 48 hpi, plated on Matrigel in the presence of either DMSO, SKI-1, or su6656. H) Quantification of three separate experiments similar to that shown in G. Asterisks indicate a p-value of less than 0.05 compared to DMSO or negative control. Double asterisks indicate a p-value of less than 0.005 compared to DMSO control.
In order to determine if this effect was due specifically to integrin β3 we used siRNA to knock down integrin β3 protein expression. Cells were transfected with siRNA 24 hours post-infection with KSHV and harvested for analysis 48 hours later (at 72 hours post-infection). Figure 6 (C and D) shows that the expression of αVβ3 integrin complexes is significantly reduced upon transfection of integrin β3 siRNA. Interestingly, αVβ3 expression is reduced more strongly in mock-infected cells (Figure 6C) than in KSHV-infected cells (Figure 6D). Mock-infected cells with control siRNA have a geometric mean fluorescence of 22.3, which drops to 3.4 in cells expressing integrin β3 siRNA, a greater than 6.5 fold decrease in expression. In contrast, in KSHV-infected cells, the geometric mean fluorescence of integrin β3 goes from 31.9 in control siRNA-expressing cells to 6.9 in β3 siRNA-expressing cells, which is still above background fluorescence levels and is a 4.6 fold decrease. This is likely due to the increased integrin β3 transcript levels in KSHV-infected cells. Integrin β3 specific siRNA impaired KSHV-induced capillary morphogenesis (Figure 6E and F, p-value = 0.003) but had little effect on mock-infected cells (a 33% versus 12% decrease in capillary formation respectively), despite the small amount of residual integrin β3 expression in KSHV-infected cells with the β3 specific siRNA. This suggests that the KSHV-induced angiogenic phenotype specifically requires integrin β3 expression.
To further confirm that integrin activation and signaling during KSHV latent infection is required for the KSHV-induced angiogenic phenotype, we next determined whether Src kinase activity is required for KSHV-induced capillary morphogenesis. Mock- and KSHV-infected cells were treated with the indicated concentrations of Src kinase inhibitor SKI-1 or DMSO alone and plated on Matrigel matrix. Similar to the effects of inhibition by the RGD-containing peptide or integrin β3 siRNA, inhibition of Src kinase had a greater impact on KSHV-infected cells than on mock-infected cells (Figure 6G and H). KSHV-infected cells treated with 5 nM SKI-1 had an approximate 5-fold decrease in the number of capillaries formed compared to DMSO-treated cells. In contrast, mock-infected cells retained nearly complete ability to organize on Matrigel. At higher concentrations of SKI-1, capillary formation of KSHV-infected endothelial cells was completely eliminated while mock-infected cells had only modest decreases in capillary formation. To further demonstrate that the effects seen were due to inhibition of Src, another Src kinase inhibitor, su6656, was used. Su6656 had a similar effect on KSHV-infected cells as SKI-1 (Figure 6G and H). Similar experiments with primary HMVECS yielded comparable results (data not shown). Thus, three different methods of inhibiting integrin signaling – RGD peptides, siRNA to integrin β3, and chemical inhibition of downstream integrin signaling – blocked capillary formation by KSHV infected endothelial cells in a three dimensional matrix more significantly than in mock infected cells.
During angiogenesis, activated endothelial cells disrupt the extracellular matrix, proliferate and migrate towards angiogenic stimuli, gain traction into new areas through increased adhesion to specific substrates, and organize into preliminary vasculature. These activities are necessary for vascularization of solid tumors and contribute to the promotion of tumor formation and metastasis. As has been described by others, KSHV is likely to promote angiogenesis in a paracrine fashion to promote new blood vessel growth in the KS tumor , , –, , , . Here we found that KSHV also directly induces angiogenic phenotypes in latently infected cells. KSHV latent infection of endothelial cells increased cell motility and cell adhesion. Therefore, angiogenic phenotypes induced in latently infected endothelial cells may promote angiogenesis in KS tumors but may also directly be involved in the activation of the endothelial tumor cells themselves.
Integrins are involved in many processes of both angiogenesis and oncogenesis. Integrins have been shown to be critical for angiogenic phenotypes such as adhesion, migration, and anchorage-independent survival (reviewed in , ). Integrins have also been shown to be important in oncogenesis of many cell types (reviewed in ). In particular, integrin αVβ3 is expressed on blood vessels in human tumor biopsy samples, but not on vessels in normal human tissues, suggesting a role in tumor-associated angiogenesis , . Furthermore, inhibition of integrins, and in particular αVβ3, can inhibit angiogenesis and promote regression of tumors by inducing apoptosis of endothelial cells –. We found that KSHV not only induces the expression of integrin β3 leading to increased surface expression of αVβ3, but latent infection also leads to the activation of αVβ3 and downstream activation of FAK and Src. Importantly, integrin signaling, specifically αVβ3, is necessary for KSHV induction of angiogenic phenotypes including cell motility and adhesion.
Regulation of angiogenesis is a complex process that involves cross talk between multiple signaling pathways. Integrin signaling complexes have been shown to interact with angiogenic growth factor receptors, such as VEGF and basic fibroblast growth factor . Specifically, interaction between αVβ3 and VEGF receptor 2 can lead to phosphorylation of the cytoplasmic tail of β3 as well as increased phosphorylation of VEGF receptor 2 in a Src-dependent fashion . Interestingly, β3 knockout mice exhibit increased pathological angiogenesis, due to increased expression and signaling through VEGF receptor 2 , . However, knock-in expression of a mutant β3 that lacks phosphorylation sites blocks VEGF-induced pathological angiogenesis in these mice . Integrin αVβ3 also plays a role in regulating the expression of VEGF; its activation can increase VEGF secretion by tumor cells . Thus, upregulation of VEGF and its receptors by KSHV likely acts in concert with increased αVβ3 signaling to promote angiogenesis.
Interestingly, KSHV-infected cells become dependent upon αVβ3 for capillary formation. Inhibition of integrin signaling and αVβ3 had small effects on the ability of uninfected endothelial cells to form capillary-like structures on Matrigel. However, during KSHV latency, capillary formation is extremely sensitive to inhibitors of αVβ3 and integrin signaling. In uninfected cells αVβ3 is localized with focal adhesions all over the cell, while in infected cells αVβ3 is localized to the focal adhesions at the periphery. It is possible that the dramatic change in focal adhesions and polymerized actin in the infected cells leads to increased reliance on αVβ3. However, whether the increased sensitivity is due to a switch in the infected cell solely to integrin signaling for capillary formation or if there is a decrease in a compensatory pathway is unknown. In either event, this provides a good target for therapy, as the latently infected cells are more sensitive than mock-infected cells to inhibitors of integrin signaling.
Integrins are also used for KSHV binding and entry into cells. It was first demonstrated that α3β1 was a receptor for KSHV on endothelial cells . Subsequently it was shown that gB binds to αVβ3 and that αVβ3 may be the dominant integrin for binding and entry . Interestingly, both of these integrins together with CD98 may form an entry complex . During binding and entry, gB binding to integrins leads to activation of the integrins and subsequent activation of FAK and focal adhesions –. While this phenomenon is similar to what we describe, we found that activation of FAK and Src also occurs during latency and, at the time points we examined, this is not due to initial binding and entry of the virus. Additionally, gB has previously been shown to only be expressed in cells undergoing lytic replication and cannot be detected at the late times post-infection that we examined . While cells undergoing lytic replication are present in our cultures, less than 1% of the cells express ORF 59, a common marker of lytic infection. Using an immunofluorescence assay we find that most of the infected cells have altered focal adhesions at the periphery. Furthermore, the bulk of the cells shift expression of αVβ3 and the bulk of the population is more motile and more adherent, indicating that the phenomenon we are examining is occurring in latently infected cells. Importantly, in cultures where we have a mixed population of uninfected and KSHV-infected cells, only the cells that have LANA expression, i.e. are latently infected, have relocalized actin to the periphery (Figure 3I) and have focal adhesions at the periphery as well (not shown). Taken together, these data all indicate that there is a factor in the latent cells that is necessary for activation of integrin signaling and induction of the angiogenic phenotypes. While paracrine factors from the low percentage of lytically infected cells could play some role in the angiogenic phenotypes described, a latent factor is still required. The viral gene or genes upregulating integrin β3 and activating αVβ3 and FAK are currently unknown and will be the focus of future studies.
Inhibition of integrins and integrin signaling has been proposed as a target for tumor therapy through inhibition of tumor cell growth itself and through inhibition of neo-angiogenesis. A number of inhibitors of integrin signaling are in clinical trials for tumors that have increased levels of integrins and specifically αVβ3 (reviewed in , ). Importantly, an inhibitor of αVβ3 is in phase3 trials for inhibition of tumor formation. Based on the studies presented here, we believe that αVβ3 may be a good target for therapy to treat KS tumors. It is difficult to target herpesvirus latency due to the limited viral gene expression. However, through a better understanding of the host cell requirements for latency new therapeutic targets can be identified. Integrin β3 induction and activation by KSHV during latency is critical for a number of phenotypes important for tumor growth and latency, making it an attractive target for therapy.
Materials and Methods
Primary human dermal microvascular endothelial cells (hDMVEC) and TIME cells  were maintained as monolayer cultures in EBM-2 medium (Lonza) supplemented with 5% fetal bovine serum, vascular endothelial growth factor, basic fibroblast growth factor, insulin-like growth factor 3, epidermal growth factor, and hydrocortisone (EGM-2 media). BCBL-1  and BJAB cells  were maintained in RPMI 1640 medium (Celgro; Mediatech, Inc.) supplemented with 10% fetal bovine serum, penicillin, streptomycin, glutamine, and β-mercaptoethanol.
Viruses and infection
KSHV inoculum was obtained from BCBL-1 cells (5×105 cells/ml) induced with 20 ng of TPA (12-O-tetradecanoylphorbol-13-acetate; Sigma)/ml. After 5 days, cells were pelleted, and the supernatant was run through a 0.45-µm-pore-size filter (Whatman). Virions were pelleted at 30,000xg for 2 h in a JA-14 rotor, Avanti-J-25 centrifuge (Beckman Coulter). The viral pellet was resuspended in EGM-2 without supplements. KSHV infections of TIME and primary hDMVEC were performed in serum-free EBM-2 supplemented with 8 ug/ml polybrene for 3 h, after which the medium was replaced with complete EGM-2. Mock infections were performed identically except that concentrated virus was omitted from the inoculum. For all experiments, infection rates were assessed by immunofluorescence using antibodies against the latency-associated nuclear antigen (LANA) and the lytic protein ORF59. In all infections performed with wild-type (wt) KSHV >85% of the cells were LANA-positive and <1% were ORF59-positive. UV inactivation of KSHV viral stocks (5×1,200 µJ) was performed in a UV Stratalinker 1800 (Stratagene).
RNA isolation and quantitative RT-PCR
Total RNA was isolated from TIME cells using the RNeasy Plus Minikit (Qiagen). One hundred or 500 ng of total RNA was used in a SuperScript III, Platinum SYBR green, one-step, quantitative reverse transcription PCR (RT-PCR; Invitrogen) according to manufacturer's protocols with the primers for either GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (forward, 5′-AAG GTG AAG GTC GGA GTC AAC G-3′; reverse, 5′-TGG AAG ATG GTG ATG GGA TTT C-3′) or integrin β3 (forward, 5′-GCA AGG ATG CAG TGA ATT GT-3′; reverse, 5′-CTT GGG ACA CTC TGG CTC TT-3′). Relative abundances of integrin β3 mRNA were normalized by the delta threshold cycle method to the abundance of GAPDH, with mock-infected TIME cells set to 1. Error bars reflect standard errors of the means (four experiments).
Cells were harvested with a cell scraper and pelleted using the Sorvall RT7 Plus centrifuge at 4°C. An aliquot of the cells was seeded onto chamber slides for immunofluorescence analysis. Cell pellets were washed once in cold phosphate-buffered saline and then resuspended in lysis buffer (20 mM Tris [pH 7.0], 2 mM EGTA, 5 mM EDTA, phosphatase inhibitors, protease inhibitors, and 1% Triton X-100). Samples were sonicated, rocked at 4°C for 30 minutes, and then spun at 6,000 x g at 4°C. Cell extracts were fractionated on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel, and the proteins were transferred electrophoretically to Immobilon P polyvinylidene difluoride membranes (Millipore) in Tris-glycine buffer (25 mM Tris, 192 mM glycine, 20% methanol). Blots were incubated with the indicated antibody (dilutions: 1∶1000 for anti-integrin β3, 1∶1000 for anti-phosphoFAK and anti-phosphoSrc; 1∶20,000 for anti-actin) and subsequently with horseradish peroxidase-conjugated goat anti-mouse or rabbit immunoglobulin G (1∶10,000; IgG; Jackson ImmunoResearch). Immunoreactive proteins were visualized by chemiluminescence using the Amersham ECL Plus Western blotting detection reagents (GE Healthcare). Differences in band intensity were quantified by densitometric methods.
Mock- or KSHV-infected TIME cells were seeded on LabTek Permanox four-well chamber slides (Fisher Scientific) and fixed with 4% (wt/vol) paraformaldehyde in phosphate-buffered saline. Immunofluorescence was performed as described previously . Briefly, cells were incubated in Tris-Buffered Saline (20 mM Tris, 150 mM NaCl, pH 7.6; TBS) containing 1% normal goat serum followed by incubation with primary antisera at a dilution of 1∶100 (anti-integrin β3; anti-vinculin) or 1∶1,000 (rabbit or rat anti-LANA; mouse anti-ORF59) diluted in TBS containing 1% BSA overnight. Cells were then incubated with fluor-conjugated secondary antibodies (goat anti-rabbit Alexa Fluor 488, goat anti-mouse Alexa Fluor 594, or goat anti-rat Alexa Fluor 488; Molecular Probes/Invitrogen; Fluorescein-conjugated phalloidin; Sigma) for 2 hours. Cells were mounted in medium containing DAPI (4′,6′-diamidino-2-phenylindole) before being viewed under a Zeiss LSM 510 Meta confocal microscope. Images were analyzed using the Zeiss Zen 2009 LE imaging software.
Transfection of siRNA
siRNA specific to integrin β3 and negative-control oligonucleotides were designed and synthesized by Ambion (Austin, TX). The following oligonucleotide sequences were used: integrin β3 (Ambion identification [ID] no. 112581; sense, 5′-GCU AAU UCU UUG ACC UGU UdTdT-3′) and negative-control siRNA (sense, 5′-AGU ACU GCU UAC GAU ACG GdTdT-3′). At 24 h post-infection, mock- or KSHV-infected TIME cells were transfected with 3 µg siRNA using Amaxa's Nucleofector kit (Cologne, Germany) according to the manufacturer's protocol. The transfection efficiency for siRNA was approximately 90% when it was assessed with 6-carboxyfluorescein-labeled negative-control siRNA. Transfected cells were harvested for analysis after an additional 2 days of incubation at 37°C.
Flow cytometric analysis
Monolayers of cells grown in 60-mm tissue culture dishes were washed once with phosphate-buffered saline (PBS) containing 0.04% EDTA and incubated with 2 ml of cell dissociation solution (Sigma, St. Louis, MO) to remove cells from plates. Cells were washed once with DMEM containing 10% FBS and fixed in 4% paraformaldehyde in TBS for 10 minutes on ice. Cells were then pelleted and resuspended in 0.5 ml TBS with 1% goat serum for 20 min on ice. Cells were pelleted and incubated with mouse anti-αVβ3 (2 µg/ml; LM609; Millipore) or mouse IGG control prepared in 0.5 ml of TBS with 1% BSA for 30 min on ice. Following incubation, cells were washed twice with TBS/1% BSA and then incubated with anti-mouse secondary antibody conjugated to Alexafluor 488 (Invitrogen; diluted 1∶200 in 0.5 ml of TBS containing 1% BSA) for 30 min on ice. The stained cells were washed twice with TBS containing 1% BSA, resuspended in 0.5 ml of TBS containing 1% BSA, and analyzed by FACScan caliber flow cytometer (Becton-Dickinson, Franklin Lakes, NJ). Data was analyzed using FloJo flow cytometry analysis software (Tree Star, Inc.; Ashland, OR).
Cell adhesion assays
Cell adhesion assays were performed using Nunc 96-well Maxisorp plates as described previously . Briefly, wells were coated with different concentrations of collagen, fibronectin, laminin, or vitronectin in TBS containing 2 mM each of CaCl2 and MgCl2 overnight at 4°C. The next day, plates were washed with TBS and blocked with 200 µl of TBS Ca/Mg containing 1% BSA for 1 hour at room temperature. Cells were resuspended in 20 mM HEPES, 150 mM NaCl, 4 mg/ml BSA (pH 7.4) and plated at 5×104 cells/well. Cells were allowed to adhere at 37°C in a humidified incubator for 1.5 hours. Nonadherent cells were gently washed off with TBS containing Ca/Mg, and the number of adherent cells was determined by measuring the standard intracellular acid phosphatase activity. For inhibition studies on fibronectin, cells were treated with GRADSPK or GRGDSPK peptides (Sigma) in HEPES buffer for 15 minutes on ice prior to plating. For inhibition studies on vitronectin, cells were treated with GRGES or GRGDS peptides (Peptides International) in HEPES buffer for 15 minutes on ice prior to plating.
For transwell assays, transwell filters (Costar 3422) were placed in a 24-well dish and coated on the bottom side with 0.5 ml of 2 µg/ml of fibronectin (BD Biosciences) in PBS overnight at 4°C. The filter was rinsed with PBS and then blocked with 0.5 ml of 2% bovine serum albumin (BSA) prepared in PBS for 1 h at room temperature. Following blocking, the filter was rinsed with PBS, 0.5 ml of serum-free DMEM medium was added to the bottom of each well. Cells were resuspended at 1×105 cells/ml in serum free medium and 0.1 ml was added to the top of each well. Each condition was done in duplicate. Following 1.5 hours in a 37°C tissue culture incubator, the cells and medium were aspirated and the upper side of the membrane wiped with a cotton swab. The cells that had migrated through the membrane and attached to the bottom of the filter were fixed with 4% paraformaldehyde and stained with crystal violet. The mean number of cells migrated through the filter was determined by counting ten high power fields (×100). For inhibition studies, cells were incubated with SKI-1 (Calbiochem) for 15 minutes on ice prior to addition to the transwell.
Three-dimensional culture of endothelial cells
Matrigel (10 mg/ml; BD Biosciences, Bedford, MA) was applied at 0.5 ml/35 mm in a tissue culture dish and incubated at 37°C for at least 30 min to harden. Mock- or KSHV-infected cells were removed using trypsin-EDTA, washed with growth medium once, and resuspended at 1.5×105 cells per ml in growth medium. Cells (1 ml) were gently added to the Matrigel-coated plates, incubated at 37°C, monitored for 6 h, and photographed in digital format using a Nikon microscope. Capillaries were defined as cellular processes connecting two bodies of cells. Ten fields of cells were counted for each condition and the mean and standard deviations were determined. For inhibition studies, GRADSPK or GRGDSPK peptides, SKI-1 (Calbiochem), or su6656 (Sigma) were added at the time of plating on Matrigel.
Conceived and designed the experiments: TAD ML. Performed the experiments: TAD KDG. Analyzed the data: TAD ML. Wrote the paper: TAD ML.
- 1. Wabinga H, Parkin D, Wabwire-Mangen F, Mugerwa J (1993) Cancer in Kampala, Uganda, in 1989-91: changes in incidence in the era of AIDS. Int J Cancer 54: 26–36.H. WabingaD. ParkinF. Wabwire-MangenJ. Mugerwa1993Cancer in Kampala, Uganda, in 1989-91: changes in incidence in the era of AIDS.Int J Cancer542636
- 2. Chokunonga E, Levy L, Bassett M, Mauchaza B, Thomas D, et al. (2000) Cancer incidence in the African population of Harare, Zimbabwe: second results from the cancer registry 1993-1995. Int J Cancer 85: 54–59.E. ChokunongaL. LevyM. BassettB. MauchazaD. Thomas2000Cancer incidence in the African population of Harare, Zimbabwe: second results from the cancer registry 1993-1995.Int J Cancer855459
- 3. Staskus K, Zhong W, Gebhard K, Herndier B, Wang H, et al. (1997) Kaposi's sarcoma-associated herpesvirus gene expression in endothelial (spindle) tumor cells. J Virol 71: 715–719.K. StaskusW. ZhongK. GebhardB. HerndierH. Wang1997Kaposi's sarcoma-associated herpesvirus gene expression in endothelial (spindle) tumor cells.J Virol71715719
- 4. Lagunoff M, Bechtel J, Venetsanakos E, Roy A, Abbey N, et al. (2002) De novo infection and serial transmission of Kaposi's sarcoma-associated herpesvirus in cultured endothelial cells. J Virol 76: 2440–2448.M. LagunoffJ. BechtelE. VenetsanakosA. RoyN. Abbey2002De novo infection and serial transmission of Kaposi's sarcoma-associated herpesvirus in cultured endothelial cells.J Virol7624402448
- 5. Bechtel JT, Liang Y, Hvidding J, Ganem D (2003) Host range of Kaposi's sarcoma-associated herpesvirus in cultured cells. J Virol 77: 6474–6481.JT BechtelY. LiangJ. HviddingD. Ganem2003Host range of Kaposi's sarcoma-associated herpesvirus in cultured cells.J Virol7764746481
- 6. Lagunoff M, Lukac DM, Ganem D (2001) Immunoreceptor tyrosine-based activation motif-dependent signaling by Kaposi's sarcoma-associated herpesvirus K1 protein: effects on lytic viral replication. J Virol 75: 5891–5898.M. LagunoffDM LukacD. Ganem2001Immunoreceptor tyrosine-based activation motif-dependent signaling by Kaposi's sarcoma-associated herpesvirus K1 protein: effects on lytic viral replication.J Virol7558915898
- 7. Wang L, Damania B (2008) Kaposi's sarcoma-associated herpesvirus confers a survival advantage to endothelial cells. Cancer Res 68: 4640–4648.L. WangB. Damania2008Kaposi's sarcoma-associated herpesvirus confers a survival advantage to endothelial cells.Cancer Res6846404648
- 8. Couty J, Lupu-Meiri M, Oron Y, Gershengorn M (2009) Kaposi's sarcoma-associated herpesvirus-G protein-coupled receptor-expressing endothelial cells exhibit reduced migration and stimulated chemotaxis by chemokine inverse agonists. J Pharmacol Exp Ther 329: 1142–1147.J. CoutyM. Lupu-MeiriY. OronM. Gershengorn2009Kaposi's sarcoma-associated herpesvirus-G protein-coupled receptor-expressing endothelial cells exhibit reduced migration and stimulated chemotaxis by chemokine inverse agonists.J Pharmacol Exp Ther32911421147
- 9. Qian L, Xie J, Ye F, Gao S (2007) Kaposi's sarcoma-associated herpesvirus infection promotes invasion of primary human umbilical vein endothelial cells by inducing matrix metalloproteinases. J Virol 81: 7001–7010.L. QianJ. XieF. YeS. Gao2007Kaposi's sarcoma-associated herpesvirus infection promotes invasion of primary human umbilical vein endothelial cells by inducing matrix metalloproteinases.J Virol8170017010
- 10. Sadagopan S, Sharma-Walia N, Veettil M, Bottero V, Levine R, et al. (2009) Kaposi's sarcoma-associated herpesvirus upregulates angiogenin during infection of human dermal microvascular endothelial cells, which induces 45S rRNA synthesis, antiapoptosis, cell proliferation, migration, and angiogenesis. J Virol 83: 3342–3364.S. SadagopanN. Sharma-WaliaM. VeettilV. BotteroR. Levine2009Kaposi's sarcoma-associated herpesvirus upregulates angiogenin during infection of human dermal microvascular endothelial cells, which induces 45S rRNA synthesis, antiapoptosis, cell proliferation, migration, and angiogenesis.J Virol8333423364
- 11. Sadagopan S, Sharma-Walia N, Veettil M, Raghu H, Sivakumar R, et al. (2007) Kaposi's sarcoma-associated herpesvirus induces sustained NF-kappaB activation during de novo infection of primary human dermal microvascular endothelial cells that is essential for viral gene expression. J Virol 81: 3949–3968.S. SadagopanN. Sharma-WaliaM. VeettilH. RaghuR. Sivakumar2007Kaposi's sarcoma-associated herpesvirus induces sustained NF-kappaB activation during de novo infection of primary human dermal microvascular endothelial cells that is essential for viral gene expression.J Virol8139493968
- 12. Sivakumar R, Sharma-Walia N, Raghu H, Veettil M, Sadagopan S, et al. (2008) Kaposi's sarcoma-associated herpesvirus induces sustained levels of vascular endothelial growth factors A and C early during in vitro infection of human microvascular dermal endothelial cells: biological implications. J Virol 82: 1759–1776.R. SivakumarN. Sharma-WaliaH. RaghuM. VeettilS. Sadagopan2008Kaposi's sarcoma-associated herpesvirus induces sustained levels of vascular endothelial growth factors A and C early during in vitro infection of human microvascular dermal endothelial cells: biological implications.J Virol8217591776
- 13. Masood R, Cesarman E, Smith DL, Gill PS, Flore O (2002) Human herpesvirus-8-transformed endothelial cells have functionally activated vascular endothelial growth factor/vascular endothelial growth factor receptor. Am J Pathol 160: 23–29.R. MasoodE. CesarmanDL SmithPS GillO. Flore2002Human herpesvirus-8-transformed endothelial cells have functionally activated vascular endothelial growth factor/vascular endothelial growth factor receptor.Am J Pathol1602329
- 14. Carroll P, Brazeau E, Lagunoff M (2004) Kaposi's sarcoma-associated herpesvirus infection of blood endothelial cells induces lymphatic differentiation. Virology 328: 7–18.P. CarrollE. BrazeauM. Lagunoff2004Kaposi's sarcoma-associated herpesvirus infection of blood endothelial cells induces lymphatic differentiation.Virology328718
- 15. Carroll P, Kenerson H, Yeung R, Lagunoff M (2006) Latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells activates hypoxia-induced factors. J Virol 80: 10802–10812.P. CarrollH. KenersonR. YeungM. Lagunoff2006Latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells activates hypoxia-induced factors.J Virol801080210812
- 16. Wang HW, Trotter MW, Lagos D, Bourboulia D, Henderson S, et al. (2004) Kaposi sarcoma herpesvirus-induced cellular reprogramming contributes to the lymphatic endothelial gene expression in Kaposi sarcoma. Nat Genet 36: 687–693.HW WangMW TrotterD. LagosD. BourbouliaS. Henderson2004Kaposi sarcoma herpesvirus-induced cellular reprogramming contributes to the lymphatic endothelial gene expression in Kaposi sarcoma.Nat Genet36687693
- 17. Hong YK, Foreman K, Shin JW, Hirakawa S, Curry CL, et al. (2004) Lymphatic reprogramming of blood vascular endothelium by Kaposi sarcoma-associated herpesvirus. Nat Genet 36: 683–685.YK HongK. ForemanJW ShinS. HirakawaCL Curry2004Lymphatic reprogramming of blood vascular endothelium by Kaposi sarcoma-associated herpesvirus.Nat Genet36683685
- 18. Sharma-Walia N, Paul AG, Bottero V, Sadagopan S, Veettil MV, et al. (2010) Kaposi's sarcoma associated herpes virus (KSHV) induced COX-2: a key factor in latency, inflammation, angiogenesis, cell survival and invasion. PLoS Pathog 6: e1000777.N. Sharma-WaliaAG PaulV. BotteroS. SadagopanMV Veettil2010Kaposi's sarcoma associated herpes virus (KSHV) induced COX-2: a key factor in latency, inflammation, angiogenesis, cell survival and invasion.PLoS Pathog6e1000777
- 19. Vart R, Nikitenko L, Lagos D, Trotter M, Cannon M, et al. (2007) Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells. Cancer Res 67: 4042–4051.R. VartL. NikitenkoD. LagosM. TrotterM. Cannon2007Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells.Cancer Res6740424051
- 20. Ye F, Blackbourn D, Mengel M, Xie J, Qian L, et al. (2007) Kaposi's sarcoma-associated herpesvirus promotes angiogenesis by inducing angiopoietin-2 expression via AP-1 and Ets1. J Virol 81: 3980–3991.F. YeD. BlackbournM. MengelJ. XieL. Qian2007Kaposi's sarcoma-associated herpesvirus promotes angiogenesis by inducing angiopoietin-2 expression via AP-1 and Ets1.J Virol8139803991
- 21. Ma T, Jham BC, Hu J, Friedman ER, Basile JR, et al. (2010) Viral G protein-coupled receptor up-regulates Angiopoietin-like 4 promoting angiogenesis and vascular permeability in Kaposi's sarcoma. Proc Natl Acad Sci U S A 107: 14363–14368.T. MaBC JhamJ. HuER FriedmanJR Basile2010Viral G protein-coupled receptor up-regulates Angiopoietin-like 4 promoting angiogenesis and vascular permeability in Kaposi's sarcoma.Proc Natl Acad Sci U S A1071436314368
- 22. Mansouri M, Rose P, Moses A, Früh K (2008) Remodeling of endothelial adherens junctions by Kaposi's sarcoma-associated herpesvirus. J Virol 82: 9615–9628.M. MansouriP. RoseA. MosesK. Früh2008Remodeling of endothelial adherens junctions by Kaposi's sarcoma-associated herpesvirus.J Virol8296159628
- 23. Guilluy C, Zhang Z, Bhende PM, Sharek L, Wang L, et al. (2011) Latent KSHV infection increases the vascular permeability of human endothelial cells. Blood 118: 5344–5354.C. GuilluyZ. ZhangPM BhendeL. SharekL. Wang2011Latent KSHV infection increases the vascular permeability of human endothelial cells.Blood11853445354
- 24. Qian L, Greene W, Ye F, Gao S (2008) Kaposi's sarcoma-associated herpesvirus disrupts adherens junctions and increases endothelial permeability by inducing degradation of VE-cadherin. J Virol 82: 11902–11912.L. QianW. GreeneF. YeS. Gao2008Kaposi's sarcoma-associated herpesvirus disrupts adherens junctions and increases endothelial permeability by inducing degradation of VE-cadherin.J Virol821190211912
- 25. Dwyer J, Le Guelte A, Galan Moya EM, Sumbal M, Carlotti A, et al. (2011) Remodeling of VE-cadherin junctions by the human herpes virus 8 G-protein coupled receptor. Oncogene 30: 190–200.J. DwyerA. Le GuelteEM Galan MoyaM. SumbalA. Carlotti2011Remodeling of VE-cadherin junctions by the human herpes virus 8 G-protein coupled receptor.Oncogene30190200
- 26. Qin Z, Dai L, Slomiany MG, Toole BP, Parsons C (2010) Direct activation of emmprin and associated pathogenesis by an oncogenic herpesvirus. Cancer Res 70: 3884–3889.Z. QinL. DaiMG SlomianyBP TooleC. Parsons2010Direct activation of emmprin and associated pathogenesis by an oncogenic herpesvirus.Cancer Res7038843889
- 27. Dai L, Bratoeva M, Toole BP, Qin Z, Parsons C (2011) KSHV activation of VEGF secretion and invasion for endothelial cells is mediated through viral upregulation of emmprin-induced signal transduction. Int J Cancer. E-pub ahead of print. L. DaiM. BratoevaBP TooleZ. QinC. Parsons2011KSHV activation of VEGF secretion and invasion for endothelial cells is mediated through viral upregulation of emmprin-induced signal transduction.Int J Cancer. E-pub ahead of print
- 28. Aoki Y, Jaffe E, Chang Y, Jones K, Teruya-Feldstein J, et al. (1999) Angiogenesis and hematopoiesis induced by Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6. Blood 93: 4034–4043.Y. AokiE. JaffeY. ChangK. JonesJ. Teruya-Feldstein1999Angiogenesis and hematopoiesis induced by Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6.Blood9340344043
- 29. Bakken T, He M, Cannon ML (2010) The phosphatase Shp2 is required for signaling by the Kaposi's sarcoma-associated herpesvirus viral GPCR in primary endothelial cells. Virology 397: 379–388.T. BakkenM. HeML Cannon2010The phosphatase Shp2 is required for signaling by the Kaposi's sarcoma-associated herpesvirus viral GPCR in primary endothelial cells.Virology397379388
- 30. Masood R, Xia G, Smith DL, Scalia P, Still JG, et al. (2005) Ephrin B2 expression in Kaposi sarcoma is induced by human herpesvirus type 8: phenotype switch from venous to arterial endothelium. Blood 105: 1310–1318.R. MasoodG. XiaDL SmithP. ScaliaJG Still2005Ephrin B2 expression in Kaposi sarcoma is induced by human herpesvirus type 8: phenotype switch from venous to arterial endothelium.Blood10513101318
- 31. Xie J, Pan H, Yoo S, Gao S (2005) Kaposi's sarcoma-associated herpesvirus induction of AP-1 and interleukin 6 during primary infection mediated by multiple mitogen-activated protein kinase pathways. J Virol 79: 15027–15037.J. XieH. PanS. YooS. Gao2005Kaposi's sarcoma-associated herpesvirus induction of AP-1 and interleukin 6 during primary infection mediated by multiple mitogen-activated protein kinase pathways.J Virol791502715037
- 32. McAllister SC, Hansen SG, Ruhl RA, Raggo CM, DeFilippis VR, et al. (2004) Kaposi sarcoma-associated herpesvirus (KSHV) induces heme oxygenase-1 expression and activity in KSHV-infected endothelial cells. Blood 103: 3465–3473.SC McAllisterSG HansenRA RuhlCM RaggoVR DeFilippis2004Kaposi sarcoma-associated herpesvirus (KSHV) induces heme oxygenase-1 expression and activity in KSHV-infected endothelial cells.Blood10334653473
- 33. Fonsato V, Buttiglieri S, Deregibus MC, Bussolati B, Caselli E, et al. (2008) PAX2 expression by HHV-8-infected endothelial cells induced a proangiogenic and proinvasive phenotype. Blood 111: 2806–2815.V. FonsatoS. ButtiglieriMC DeregibusB. BussolatiE. Caselli2008PAX2 expression by HHV-8-infected endothelial cells induced a proangiogenic and proinvasive phenotype.Blood11128062815
- 34. Cai Q, Murakami M, Si H, Robertson E (2007) A potential alpha-helix motif in the amino terminus of LANA encoded by Kaposi's sarcoma-associated herpesvirus is critical for nuclear accumulation of HIF-1alpha in normoxia. J Virol 81: 10413–10423.Q. CaiM. MurakamiH. SiE. Robertson2007A potential alpha-helix motif in the amino terminus of LANA encoded by Kaposi's sarcoma-associated herpesvirus is critical for nuclear accumulation of HIF-1alpha in normoxia.J Virol811041310423
- 35. Shin Y, Joo C, Gack M, Lee H, Jung J (2008) Kaposi's sarcoma-associated herpesvirus viral IFN regulatory factor 3 stabilizes hypoxia-inducible factor-1 alpha to induce vascular endothelial growth factor expression. Cancer Res 68: 1751–1759.Y. ShinC. JooM. GackH. LeeJ. Jung2008Kaposi's sarcoma-associated herpesvirus viral IFN regulatory factor 3 stabilizes hypoxia-inducible factor-1 alpha to induce vascular endothelial growth factor expression.Cancer Res6817511759
- 36. Wang L, Dittmer D, Tomlinson C, Fakhari F, Damania B (2006) Immortalization of primary endothelial cells by the K1 protein of Kaposi's sarcoma-associated herpesvirus. Cancer Res 66: 3658–3666.L. WangD. DittmerC. TomlinsonF. FakhariB. Damania2006Immortalization of primary endothelial cells by the K1 protein of Kaposi's sarcoma-associated herpesvirus.Cancer Res6636583666
- 37. Boshoff C, Endo Y, Collins P, Takeuchi Y, Reeves J, et al. (1997) Angiogenic and HIV-inhibitory functions of KSHV-encoded chemokines. Science 278: 290–294.C. BoshoffY. EndoP. CollinsY. TakeuchiJ. Reeves1997Angiogenic and HIV-inhibitory functions of KSHV-encoded chemokines.Science278290294
- 38. Stine J, Wood C, Hill M, Epp A, Raport C, et al. (2000) KSHV-encoded CC chemokine vMIP-III is a CCR4 agonist, stimulates angiogenesis, and selectively chemoattracts TH2 cells. Blood 95: 1151–1157.J. StineC. WoodM. HillA. EppC. Raport2000KSHV-encoded CC chemokine vMIP-III is a CCR4 agonist, stimulates angiogenesis, and selectively chemoattracts TH2 cells.Blood9511511157
- 39. Arvanitakis L, Geras-Raaka E, Varma A, Gershengorn M, Cesarman E (1997) Human herpesvirus KSHV encodes a constitutively active G-protein-coupled receptor linked to cell proliferation. Nature 385: 347–350.L. ArvanitakisE. Geras-RaakaA. VarmaM. GershengornE. Cesarman1997Human herpesvirus KSHV encodes a constitutively active G-protein-coupled receptor linked to cell proliferation.Nature385347350
- 40. Montaner S, Sodhi A, Pece S, Mesri E, Gutkind J (2001) The Kaposi's sarcoma-associated herpesvirus G protein-coupled receptor promotes endothelial cell survival through the activation of Akt/protein kinase B. Cancer Res 61: 2641–2648.S. MontanerA. SodhiS. PeceE. MesriJ. Gutkind2001The Kaposi's sarcoma-associated herpesvirus G protein-coupled receptor promotes endothelial cell survival through the activation of Akt/protein kinase B.Cancer Res6126412648
- 41. Sodhi A, Montaner S, Patel V, Zohar M, Bais C, et al. (2000) The Kaposi's sarcoma-associated herpes virus G protein-coupled receptor up-regulates vascular endothelial growth factor expression and secretion through mitogen-activated protein kinase and p38 pathways acting on hypoxia-inducible factor 1alpha. Cancer Res 60: 4873–4880.A. SodhiS. MontanerV. PatelM. ZoharC. Bais2000The Kaposi's sarcoma-associated herpes virus G protein-coupled receptor up-regulates vascular endothelial growth factor expression and secretion through mitogen-activated protein kinase and p38 pathways acting on hypoxia-inducible factor 1alpha.Cancer Res6048734880
- 42. Bais C, Santomasso B, Coso O, Arvanitakis L, Raaka E, et al. (1998) G-protein-coupled receptor of Kaposi's sarcoma-associated herpesvirus is a viral oncogene and angiogenesis activator. Nature 391: 86–89.C. BaisB. SantomassoO. CosoL. ArvanitakisE. Raaka1998G-protein-coupled receptor of Kaposi's sarcoma-associated herpesvirus is a viral oncogene and angiogenesis activator.Nature3918689
- 43. Bais C, Van Geelen A, Eroles P, Mutlu A, Chiozzini C, et al. (2003) Kaposi's sarcoma associated herpesvirus G protein-coupled receptor immortalizes human endothelial cells by activation of the VEGF receptor-2/KDR. Cancer Cell 3: 131–143.C. BaisA. Van GeelenP. ErolesA. MutluC. Chiozzini2003Kaposi's sarcoma associated herpesvirus G protein-coupled receptor immortalizes human endothelial cells by activation of the VEGF receptor-2/KDR.Cancer Cell3131143
- 44. Shan B, Morris CA, Zhuo Y, Shelby BD, Levy DR, et al. (2007) Activation of proMMP-2 and Src by HHV8 vGPCR in human pulmonary arterial endothelial cells. J Mol Cell Cardiol 42: 517–525.B. ShanCA MorrisY. ZhuoBD ShelbyDR Levy2007Activation of proMMP-2 and Src by HHV8 vGPCR in human pulmonary arterial endothelial cells.J Mol Cell Cardiol42517525
- 45. Dourmishev L, Dourmishev A, Palmeri D, Schwartz R, Lukac D (2003) Molecular genetics of Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) epidemiology and pathogenesis. Microbiol Mol Biol Rev. 67. : 175–212.L. DourmishevA. DourmishevD. PalmeriR. SchwartzD. Lukac2003Molecular genetics of Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) epidemiology and pathogenesis.Microbiol Mol Biol Rev67175212table of contents. table of contents.
- 46. Murakami Y, Yamagoe S, Noguchi K, Takebe Y, Takahashi N, et al. (2006) Ets-1-dependent expression of vascular endothelial growth factor receptors is activated by latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus through interaction with Daxx. J Biol Chem 281: 28113–28121.Y. MurakamiS. YamagoeK. NoguchiY. TakebeN. Takahashi2006Ets-1-dependent expression of vascular endothelial growth factor receptors is activated by latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus through interaction with Daxx.J Biol Chem2812811328121
- 47. Mitra S, Schlaepfer D (2006) Integrin-regulated FAK-Src signaling in normal and cancer cells. Curr Opin Cell Biol 18: 516–523.S. MitraD. Schlaepfer2006Integrin-regulated FAK-Src signaling in normal and cancer cells.Curr Opin Cell Biol18516523
- 48. Avraamides CJ, Garmy-Susini B, Varner JA (2008) Integrins in angiogenesis and lymphangiogenesis. Nat Rev Cancer 8: 604–617.CJ AvraamidesB. Garmy-SusiniJA Varner2008Integrins in angiogenesis and lymphangiogenesis.Nat Rev Cancer8604617
- 49. Hehlgans S, Haase M, Cordes N (2007) Signalling via integrins: implications for cell survival and anticancer strategies. Biochim Biophys Acta 1775: 163–180.S. HehlgansM. HaaseN. Cordes2007Signalling via integrins: implications for cell survival and anticancer strategies.Biochim Biophys Acta1775163180
- 50. Gasparini G, Brooks PC, Biganzoli E, Vermeulen PB, Bonoldi E, et al. (1998) Vascular integrin alpha(v)beta3: a new prognostic indicator in breast cancer. Clin Cancer Res 4: 2625–2634.G. GaspariniPC BrooksE. BiganzoliPB VermeulenE. Bonoldi1998Vascular integrin alpha(v)beta3: a new prognostic indicator in breast cancer.Clin Cancer Res426252634
- 51. Bello L, Zhang J, Nikas DC, Strasser JF, Villani RM, et al. (2000) Alpha(v)beta3 and alpha(v)beta5 integrin expression in meningiomas. Neurosurgery 47: 1185–1195.L. BelloJ. ZhangDC NikasJF StrasserRM Villani2000Alpha(v)beta3 and alpha(v)beta5 integrin expression in meningiomas.Neurosurgery4711851195
- 52. Brooks PC, Clark RA, Cheresh DA (1994) Requirement of vascular integrin alpha v beta 3 for angiogenesis. Science 264: 569–571.PC BrooksRA ClarkDA Cheresh1994Requirement of vascular integrin alpha v beta 3 for angiogenesis.Science264569571
- 53. Brooks PC, Montgomery AM, Rosenfeld M, Reisfeld RA, Hu T, et al. (1994) Integrin alpha v beta 3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels. Cell 79: 1157–1164.PC BrooksAM MontgomeryM. RosenfeldRA ReisfeldT. Hu1994Integrin alpha v beta 3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels.Cell7911571164
- 54. Brooks PC, Strömblad S, Klemke R, Visscher D, Sarkar FH, et al. (1995) Antiintegrin alpha v beta 3 blocks human breast cancer growth and angiogenesis in human skin. J Clin Invest 96: 1815–1822.PC BrooksS. StrömbladR. KlemkeD. VisscherFH Sarkar1995Antiintegrin alpha v beta 3 blocks human breast cancer growth and angiogenesis in human skin.J Clin Invest9618151822
- 55. Sheu JR, Yen MH, Kan YC, Hung WC, Chang PT, et al. (1997) Inhibition of angiogenesis in vitro and in vivo: comparison of the relative activities of triflavin, an Arg-Gly-Asp-containing peptide and anti-alpha(v)beta3 integrin monoclonal antibody. Biochim Biophys Acta 1336: 445–454.JR SheuMH YenYC KanWC HungPT Chang1997Inhibition of angiogenesis in vitro and in vivo: comparison of the relative activities of triflavin, an Arg-Gly-Asp-containing peptide and anti-alpha(v)beta3 integrin monoclonal antibody.Biochim Biophys Acta1336445454
- 56. Garrigues HJ, Rubinchikova YE, Dipersio CM, Rose TM (2008) Integrin alphaVbeta3 Binds to the RGD motif of glycoprotein B of Kaposi's sarcoma-associated herpesvirus and functions as an RGD-dependent entry receptor. J Virol 82: 1570–1580.HJ GarriguesYE RubinchikovaCM DipersioTM Rose2008Integrin alphaVbeta3 Binds to the RGD motif of glycoprotein B of Kaposi's sarcoma-associated herpesvirus and functions as an RGD-dependent entry receptor.J Virol8215701580
- 57. Desgrosellier JS, Barnes LA, Shields DJ, Huang M, Lau SK, et al. (2009) An integrin alpha(v)beta(3)-c-Src oncogenic unit promotes anchorage-independence and tumor progression. Nat Med 15: 1163–1169.JS DesgrosellierLA BarnesDJ ShieldsM. HuangSK Lau2009An integrin alpha(v)beta(3)-c-Src oncogenic unit promotes anchorage-independence and tumor progression.Nat Med1511631169
- 58. Dyson OF, Oxendine TL, Hamden KE, Ford PW, Akula SM (2008) Differential regulation of the attachment of Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human B cells to extracellular matrix by KSHV-encoded gB and cellular alphaV integrins. Cell Microbiol 10: 1546–1558.OF DysonTL OxendineKE HamdenPW FordSM Akula2008Differential regulation of the attachment of Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human B cells to extracellular matrix by KSHV-encoded gB and cellular alphaV integrins.Cell Microbiol1015461558
- 59. Dyson O, Bryan B, Lambert P, Ford P, Akula S (2007) Beta1 integrins mediate tubule formation induced by supernatants derived from KSHV-infected cells. Intervirology 50: 245–253.O. DysonB. BryanP. LambertP. FordS. Akula2007Beta1 integrins mediate tubule formation induced by supernatants derived from KSHV-infected cells.Intervirology50245253
- 60. Desgrosellier JS, Cheresh DA (2010) Integrins in cancer: biological implications and therapeutic opportunities. Nat Rev Cancer 10: 9–22.JS DesgrosellierDA Cheresh2010Integrins in cancer: biological implications and therapeutic opportunities.Nat Rev Cancer10922
- 61. Mahabeleshwar GH, Feng W, Reddy K, Plow EF, Byzova TV (2007) Mechanisms of integrin-vascular endothelial growth factor receptor cross-activation in angiogenesis. Circ Res 101: 570–580.GH MahabeleshwarW. FengK. ReddyEF PlowTV Byzova2007Mechanisms of integrin-vascular endothelial growth factor receptor cross-activation in angiogenesis.Circ Res101570580
- 62. Reynolds LE, Wyder L, Lively JC, Taverna D, Robinson SD, et al. (2002) Enhanced pathological angiogenesis in mice lacking beta3 integrin or beta3 and beta5 integrins. Nat Med 8: 27–34.LE ReynoldsL. WyderJC LivelyD. TavernaSD Robinson2002Enhanced pathological angiogenesis in mice lacking beta3 integrin or beta3 and beta5 integrins.Nat Med82734
- 63. Reynolds AR, Reynolds LE, Nagel TE, Lively JC, Robinson SD, et al. (2004) Elevated Flk1 (vascular endothelial growth factor receptor 2) signaling mediates enhanced angiogenesis in beta3-integrin-deficient mice. Cancer Res 64: 8643–8650.AR ReynoldsLE ReynoldsTE NagelJC LivelySD Robinson2004Elevated Flk1 (vascular endothelial growth factor receptor 2) signaling mediates enhanced angiogenesis in beta3-integrin-deficient mice.Cancer Res6486438650
- 64. Mahabeleshwar GH, Feng W, Phillips DR, Byzova TV (2006) Integrin signaling is critical for pathological angiogenesis. J Exp Med 203: 2495–2507.GH MahabeleshwarW. FengDR PhillipsTV Byzova2006Integrin signaling is critical for pathological angiogenesis.J Exp Med20324952507
- 65. De S, Razorenova O, McCabe NP, O'Toole T, Qin J, et al. (2005) VEGF-integrin interplay controls tumor growth and vascularization. Proc Natl Acad Sci U S A 102: 7589–7594.S. DeO. RazorenovaNP McCabeT. O'TooleJ. Qin2005VEGF-integrin interplay controls tumor growth and vascularization.Proc Natl Acad Sci U S A10275897594
- 66. Nemerow G, Cheresh D (2002) Herpesvirus hijacks an integrin. Nat Cell Biol 4: E69–71.G. NemerowD. Cheresh2002Herpesvirus hijacks an integrin.Nat Cell Biol4E6971
- 67. Veettil M, Sadagopan S, Sharma-Walia N, Wang F, Raghu H, et al. (2008) Kaposi's sarcoma-associated herpesvirus forms a multimolecular complex of integrins (alphaVbeta5, alphaVbeta3, and alpha3beta1) and CD98-xCT during infection of human dermal microvascular endothelial cells, and CD98-xCT is essential for the postentry stage of infection. J Virol 82: 12126–12144.M. VeettilS. SadagopanN. Sharma-WaliaF. WangH. Raghu2008Kaposi's sarcoma-associated herpesvirus forms a multimolecular complex of integrins (alphaVbeta5, alphaVbeta3, and alpha3beta1) and CD98-xCT during infection of human dermal microvascular endothelial cells, and CD98-xCT is essential for the postentry stage of infection.J Virol821212612144
- 68. Krishnan H, Sharma-Walia N, Streblow D, Naranatt P, Chandran B (2006) Focal adhesion kinase is critical for entry of Kaposi's sarcoma-associated herpesvirus into target cells. J Virol 80: 1167–1180.H. KrishnanN. Sharma-WaliaD. StreblowP. NaranattB. Chandran2006Focal adhesion kinase is critical for entry of Kaposi's sarcoma-associated herpesvirus into target cells.J Virol8011671180
- 69. Sharma-Walia N, Naranatt P, Krishnan H, Zeng L, Chandran B (2004) Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 envelope glycoprotein gB induces the integrin-dependent focal adhesion kinase-Src-phosphatidylinositol 3-kinase-rho GTPase signal pathways and cytoskeletal rearrangements. J Virol 78: 4207–4223.N. Sharma-WaliaP. NaranattH. KrishnanL. ZengB. Chandran2004Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 envelope glycoprotein gB induces the integrin-dependent focal adhesion kinase-Src-phosphatidylinositol 3-kinase-rho GTPase signal pathways and cytoskeletal rearrangements.J Virol7842074223
- 70. Wang F, Akula S, Sharma-Walia N, Zeng L, Chandran B (2003) Human herpesvirus 8 envelope glycoprotein B mediates cell adhesion via its RGD sequence. J Virol 77: 3131–3147.F. WangS. AkulaN. Sharma-WaliaL. ZengB. Chandran2003Human herpesvirus 8 envelope glycoprotein B mediates cell adhesion via its RGD sequence.J Virol7731313147
- 71. Venetsanakos E, Mirza A, Fanton C, Romanov SR, Tlsty T, et al. (2002) Induction of tubulogenesis in telomerase-immortalized human microvascular endothelial cells by glioblastoma cells. Exp Cell Res 273: 21–33.E. VenetsanakosA. MirzaC. FantonSR RomanovT. Tlsty2002Induction of tubulogenesis in telomerase-immortalized human microvascular endothelial cells by glioblastoma cells.Exp Cell Res2732133
- 72. Renne R, Zhong W, Herndier B, McGrath M, Abbey N, et al. (1996) Lytic growth of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) in culture. Nat Med 2: 342–346.R. RenneW. ZhongB. HerndierM. McGrathN. Abbey1996Lytic growth of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) in culture.Nat Med2342346
- 73. Chen L, Lagunoff M (2005) Establishment and maintenance of Kaposi's sarcoma-associated herpesvirus latency in B cells. J Virol 79: 14383–14391.L. ChenM. Lagunoff2005Establishment and maintenance of Kaposi's sarcoma-associated herpesvirus latency in B cells.J Virol791438314391
- 74. Rothermel T, Engelhardt B, Sheibani N (2005) Polyoma virus middle-T-transformed PECAM-1 deficient mouse brain endothelial cells proliferate rapidly in culture and form hemangiomas in mice. J Cell Physiol 202: 230–239.T. RothermelB. EngelhardtN. Sheibani2005Polyoma virus middle-T-transformed PECAM-1 deficient mouse brain endothelial cells proliferate rapidly in culture and form hemangiomas in mice.J Cell Physiol202230239