Homologous recombination (HR) mediates one of the major mechanisms of trypanosome antigenic variation by placing a different variant surface glycoprotein (VSG) gene under the control of the active expression site (ES). It is believed that the majority of VSG switching events occur by duplicative gene conversion, but only a few DNA repair genes that are central to HR have been assigned a role in this process. Gene conversion events that are associated with crossover are rarely seen in VSG switching, similar to mitotic HR. In other organisms, TOPO3α (Top3 in yeasts), a type IA topoisomerase, is part of a complex that is involved in the suppression of crossovers. We therefore asked whether a related mechanism might suppress VSG recombination. Using a set of reliable recombination and switching assays that could score individual switching mechanisms, we discovered that TOPO3α function is conserved in Trypanosoma brucei and that TOPO3α plays a critical role in antigenic switching. Switching frequency increased 10–40-fold in the absence of TOPO3α and this hyper-switching phenotype required RAD51. Moreover, the preference of 70-bp repeats for VSG recombination was mitigated, while homology regions elsewhere in ES were highly favored, in the absence of TOPO3α. Our data suggest that TOPO3α may remove undesirable recombination intermediates constantly arising between active and silent ESs, thereby balancing ES integrity against VSG recombination.
Trypanosoma brucei, the causative agent of African sleeping sickness, escapes the host immune response through a mechanism known as the antigenic variation. Each individual trypanosome expresses a single species of surface antigenic protein at any time yet possesses an infinite potential to express different surface antigens by transcriptional and recombinatorial switching. Periodic switching to a different antigen allows parasites to escape the antibody-mediated host immune response and causes chronic infection, eventually overwhelming the host's immune system and leading to death. DNA recombination factors are critical for the protection of chromosome integrity. One of the major antigen-switching mechanisms exploits particular recombination pathways to achieve its purpose. We have used a new switching assay to study a regulator of recombination and to demonstrate that antigenic variation is a complex mechanism balancing chromosome integrity and antigen diversity by suppressing and promoting particular recombination events. Recombination is used in evasion or virulence mechanisms by several pathogens. Exploring how Trypanosoma brucei manipulates the recombination machinery to gain advantage against their host will help us understand pathogenesis in various organisms and may reveal weaknesses that can be exploited to control infectivity and virulence.
Citation: Kim H-S, Cross GAM (2010) TOPO3α Influences Antigenic Variation by Monitoring Expression-Site-Associated VSG Switching in Trypanosoma brucei. PLoS Pathog 6(7): e1000992. doi:10.1371/journal.ppat.1000992
Editor: John M. Mansfield, University of Wisconsin-Madison, United States of America
Received: August 4, 2009; Accepted: June 8, 2010; Published: July 8, 2010
Copyright: © 2010 Kim, Cross. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by a Rockefeller University Women in Science fellowship to HK and by grant number R01AI021729 from the National Institute of Allergy and Infectious Diseases (NIAID) of the U.S. National Institutes of Health (NIH). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIAID or the NIH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Trypanosoma brucei proliferates in the bloodstream of its mammalian host and periodically escapes the antibody-mediated immune response. A single species of variant surface glycoprotein (VSG) is expressed at a given time, from among >1,000 VSG genes and pseudogenes , , and ~10 million VSG molecules homogenously coat the surface of a parasite. Switching the expressed VSG causes antigenic variation (reviewed in –).
VSG genes are found in 15 expression sites (ESs) — polycistronic transcription units that are transcribed by RNA Polymerase I , – — of the Lister 427 strain . These VSGs are located 40–60 kb downstream of their ES promoters and are flanked by 70-bp and telomere repeat sequences. Several expression-site-associated genes (ESAGs) with mostly unknown functions, and ESAG and VSG pseudogenes, are located between the promoter and the 70-bp repeat region. Only one ES is transcriptionally active at any time and the rest are silent. Many VSGs are found upstream of telomere repeats in minichromosomes but most are thought to reside in ‘telomere-distal’ arrays. Minichromosomal and telomere-distal VSGs lack promoters, but small numbers of 70-bp repeats are present upstream of these VSGs.
By analyzing switched variants, two major pathways of antigenic switching have been identified in T. brucei: in situ ES transcription switching and recombination-mediated switching , , . In situ switching occurs by silencing the active ES and activating a silent ES, without DNA rearrangement , . Recombination-mediated switching occurs mainly by gene conversion (GC) and can involve just the VSG or larger regions of the ES. VSG GC can occur by recombination between the active VSG and a silent ES-associated VSG, a minichromosomal VSG, or a telomere-distal VSG –. Gene conversion between larger regions can result in the duplication of an entire ES, including its VSG . Crossover switches, where two VSGs are exchanged, have also been observed infrequently –.
Deficiency of RAD51 or RAD51-3 (RAD51-related gene), or BRCA2, a mediator for RAD51 filament formation, decreased switching frequency in T. brucei –. Mre11 is essential for DNA damage response, as a sensor of double strand breaks (DSBs) that can be repaired by homologous recombination (HR) or non-homologous end joining (NHEJ) –. As in yeast and mammals, T. brucei mre11 null mutants exhibited growth defects, hypersensitivity to a DNA damaging agent, and gross chromosomal rearrangements (GCR), but no detectable decrease in VSG switching , , indicating that, although antigenic variation shares core features with classic HR, specific roles for recombination factors in antigenic variation remain to be determined.
Mitotic crossover can be detrimental, leading to unequal exchanges. Sgs1, a RecQ family helicase in yeast, is one of the major factors that control spontaneous crossovers . Sgs1 forms a complex with Top3 (type IA topoisomerase) and Rmi1 (RecQ-mediated genome instability), and plays major roles in the suppression of genome instability by influencing mitotic and meiotic recombination, replication fork stability, and telomere maintenance –. At least one mechanism of crossover-suppression appears to involve ‘dissolution’ of double Holliday Junction (dHJ) intermediates. Sgs1-Top3-Rmi1, also known as the RTR (RecQ-Top3-Rmi1) complex, is well conserved in humans as the BLM (Bloom mutated)-TOPO3α-BLAP75/18 (Bloom associated protein 75kDa/18kDa, or RMI1/2). Mutations in any member of the RTR complex increase recombination frequency and crossover , , –. Defects in the BLM pathway are associated with elevated levels of sister chromatid exchanges (SCEs), chromosomal breaks and translocations , , –.
Crossover has rarely been observed in VSG switching. Suppression of crossover is intriguing because, in principle, the outcome of duplicative VSG conversion holds no apparent advantage over crossover events, as re-expressing a VSG, either exchanged or duplicated, will be lethal in vivo. Given the similarities between HR and VSG switching, we hypothesized that certain yeast hyper-recombination mutants could be hyper-switchers in trypanosomes. Using new recombination and VSG switching assays, we took advantage of a potential member of T. brucei Sgs1 pathway, TbTOPO3α (Tb11.01.1280), to get better insights on how trypanosomes employ recombination factors to control antigenic variation.
Type 1A toposiomerase TOPO3α is conserved in Trypanosoma brucei
Type IA topoisomerases cleave DNA by covalent attachment of one of the DNA strands through a 5′phosphodiester bond to a tyrosine residue in their catalytic domains . In many organisms, type IA topoisomerases function in cooperation with helicases, as a combination of Top3-Sgs1 in yeasts and TOPO3α-BLM in humans. T. brucei expresses a 102.5-kDa TOPO3α protein with 918 amino acids. Figure 1 shows an alignment of TbTOPO3α with human TOPO3α and S. cerevisiae and S. pombe Top3. The primary sequences are well aligned at the N-terminal catalytic domain including the active site tyrosine. Both E. coli Top1 and human TOPO3α contain Zn-binding motif(s) in their C-terminal regions. E. coli Top3 and two yeast Top3 lack a Zn-binding domain (reviewed in ). TbTOPO3α seems to have a Zn-binding motif in the C-terminus (four cysteine residues written in red), although this region does not align well with human TOPO3α. The sequences of TOPO3α are very well conserved in T. brucei, T. cruzi and Leishmania major (Supporting Figure S1). T. brucei also has a type IA TOPO3β (http://www.genedb.org/genedb/tryp), but its function has not been studied.
The colored boxes indicate domains found in SMART (Simple Modular Architecture Research Tool): yellow box, TOPRIM (topoisomerase-primase) domain; purple, TOP1Bc (bacterial DNA topoisomerase I ATP-binding domain); red, TOP1Ac (bacterial DNA topoisomerase I DNA binding domain); green, Zf-C4 (zinc-finger domain). The catalytic tyrosine residue (Y) is written in yellow. Four cysteine residues are written in red in a green box.
topo3α−/− exhibits a minor growth defect in T. brucei
To explore the role of TOPO3α, we sequentially deleted both alleles. We used deletion-cassettes containing hygromycin (HYG) or puromycin (PUR) resistance genes fused to Herpes simplex virus thymidine kinase (HSVTK or TK) and flanked by loxP sites, allowing the markers to be removed by transient expression of Cre-recombinase and reused . Deletion of both alleles was confirmed by PCR analyses (Supporting Figure S2).
Loss of Top3 causes a severe growth defect in budding yeast and is lethal in fission yeast , . The absence of TOPO3α or TOPO3β results in embryonic lethality or shortened life span in mice , . In contrast, TOPO3α null mutants exhibited only a minor growth defect in T. brucei (Figure 2A).
(A) topo3α−/− shows a minor growth defect. Wild-type, topo3α−/+ and topo3α−/− cells were diluted to 10,000 cells/ml and cells were counted after two days of incubation. This was repeated twice. (B) and (C) topo3α−/− is sensitive to phleomycin and HU. Cells were treated with the indicated concentrations of phleomycin (B) or HU (C) and single cells were distributed into 96-well plates. Percent viability was determined by normalizing to untreated samples. The same strains used in Figure 2A were analyzed in these experiments.
Tbtopo3α mutants are sensitive to phleomycin and hydoxyurea
Yeast Top3 is important for the maintenance of genome integrity. top3 mutants are sensitive to DNA-damaging agents and show defects in the activation of the cell-cycle checkpoint kinase Rad53 (CHK2 in mammals), in response to genotoxic stresses –. We therefore asked whether T. brucei TOPO3α is required for the DNA damage response, by assessing sensitivity to the DSB-inducing agent phleomycin or the replication inhibitor hydroxyurea (HU). Cells were treated with phleomycin for 24 hours and single cells were distributed in 96-well plates. The color of the medium turns from red to yellow when the culture becomes saturated. Yellow wells were counted after 7–8 days and the percent viability was calculated by normalizing to the untreated samples. In the null mutants, viability was reduced by 3-fold at 0.3 µg/ml and 10-fold at 0.6 µg/ml phleomycin (Figure 2B). Viability of the HU-treated null mutants was reduced by 3-fold in 0.04 mM HU (Figure 2C). topo3α−/+ was comparable to the wild type in both experiments. We conclude that TOPO3α is required for the response to DNA damage and replication block, similar to the roles of yeast Top3.
Tbtopo3α−/− is a ‘hyper-rec’
top3 was isolated as a hyper-recombination (‘hyper-rec’) mutant in a genetic screen designed to identify mutations that increase recombination frequency at SUP4-o locus in budding yeast . We therefore hypothesized that Tbtopo3α could be a ‘hyper-rec’ mutant and this phenotype could be reflected in the frequency of recombination-mediated antigenic switching.
To test whether TOPO3α deficiency increases recombination frequency, we established a new recombination assay. Thus far, transfection-based recombination assays have been predominantly used, in which trypanosomes are transfected with linear DNA containing a selection marker flanked by targeting sequences, and the recombination frequency is calculated from the number of drug-resistant clones that arise. Although this method can give reliable measurements, it requires a high rate of recombination at the target site and is subject to variations in transfection efficiency. To allow a more convenient, natural and reliable measure of recombination efficiency, we established an assay (Figure 3A) in which HYG-TK can replace one allele of what we will call TbURA3 (the bifunctional orotidine-5-phosphate decarboxylase/orotate phosphoribosyltransferase Tb927.5.3810) on chromosome V. The frequency of loss of either the HYG-TK or TbURA3 allele represents the rate of gene conversion at this locus. The frequency of HYG-TK loss can be measured with gancyclovir (GCV), a nucleoside analog, as only the cells that had lost the TK gene can grow in the presence of GCV. The loss of TbURA3 can be measured with 5-FOA (5-fluoroorotic acid), as only the ura3− cells can grow in the presence of 5-FOA.
(A) Schematic diagram of recombination assay. Gene conversion frequency was determined by using two counter-selectable markers, TK and URA3. One allele of TbURA3 was replaced with HYG-TK. The frequency of HYG-TK loss can be measured with GCV. Only the cells that had lost the TK gene can grow in the presence of GCV. Loss of TbURA3 can be measured with 5-FOA, as only the ura3− cells can grow in its presence. (B) TOPO3α deficiency increases gene conversion frequency. Overall GC frequencies (GCVR and FOAR) were plotted. Error bars indicate standard deviation.
To remove the HYG-TK and PUR-TK markers that were used for the deletion of TOPO3α, Cre-recombinase was transiently transfected into the topo3α−/− cells and GCVR HYGS PURS clones were selected (Supporting Figure S2). One allele of TbURA3 was then replaced with HYG-TK and the targeting was confirmed by PCR. Gene-conversion frequencies were determined by counting total GCRR and FOAR cells, in three wild-type and five topo3α−/− independent HYG-TK clones. As shown in Figure 3B, Tbtopo3α gave indeed a hyper-recombination phenotype. Total gene-conversion frequency was increased 6-fold in topo3α−/− (5.12±0.15×10−5) compared to the wild type (0.87±0.70×10−5).
Tbtopo3α−/− is a VSG ‘hyper-switcher’ and this phenotype requires RAD51
To investigate the roles for TOPO3α in VSG switching, we generated a VSG switching reporter strain in which we could easily measure switching frequency and score different switching mechanisms. As illustrated in Figure 4, the parental strain expresses VSG 427-2 (221) in ES1, which was doubly marked with a blasticidin-resistance gene (BSD) downstream of the promoter and PUR-TK at the 3′ end of the 70-bp repeat region, without disrupting the co-transposed region (CTR), disruption of which has been shown to induce rapid VSG switching . The 5′ boundaries for recombination-mediated VSG switching have been mapped at regions upstream of CTRs that are located between the 70-bp repeats and the VSG. Therefore, the PUR-TK gene will either be lost or repressed in switched cells. This will allow switchers, but not the parental cells, to grow in the presence of GCV.
Parental cells have VSG 427-2-ES1 active. ES1 was doubly marked with BSD and PUR-TK. PUR-TK will either be lost (VSG and ES GC) or repressed (in situ and crossover) in switched cells. Therefore, only the switchers can grow in the presence of GCV. Switchers can later be distinguished by analyzing VSG 427-2 and BSD as specified in Table 1.
Doubly marked wild-type and topo3α−/− cells were maintained in media containing blasticidin and puromycin, to exclude switchers from the starting population. The cells were allowed to switch in the absence of drugs for 3–4 days. Un-switched VSG 427-2-expressing cells were depleted by magnetic-activated cell sorting (MACS) . The column flow-through, highly enriched with switchers, was serially diluted in medium containing 4 µg/ml GCV and distributed into 96-well plates. Switching frequency was determined as the ratio of GCV-resistant cells to the total number of cells prepared for the MACS column experiments. We analyzed three independent wild-type cultures and four topo3α−/− cultures. As shown in Figure 5A, TOPO3α deficiency caused a 10–40-fold increase in switching frequency (26±16×10−5) compared to wild type (1.01±0.45×10−5). This is the only known example of an increase in VSG switching frequency when a repair factor is deleted. To confirm that the column-mediated depletion of VSG 427-2-expressing cells was not biasing our results, other batches of cells were directly diluted in GCV-containing media and distributed into 96-well plates. Switching frequency was 10–30-fold increased in the absence of TOPO3α (data not shown).
(A) TOPO3α deficiency increased total switching frequency by 10–40-fold. Switchers were enriched as described previously  and selected in GCV-containing media. The switching frequency was determined by counting GCVR clones. (B) Hyper-switching phenotype is associated with TOPO3α deficiency. Switching frequency was measured in GCV-containing media without the column-enrichment. (C) Hyper-switching phenotype requires RAD51. Switching frequency of wild type, topo3α−/−, rad51−/− and topo3α−/−rad51−/− was determined as described above (Figure 5A). Error bars indicate standard deviation. (*) The same data as presented in Figure 5A.
We have determined the switching frequency in a strain without the TK marker but with a PUR marker inserted downstream of VSG 427-2 and obtained similar frequencies, ~1×10−5, in wild type. In two different but closely related cell lines, with the same genotype except that one line has PUR-TK inserted at the 70-bp repeat and the other just PUR, again similar switching frequencies, ~1×10−5, were observed  (personal communication with Nina Papavasiliou).
Reintroduction of wild-type TOPO3α complemented the hyper-switching phenotype of topo3α−/− (−/−/+ in Figure 5B), confirming that this phenotype is associated with the TOPO3α deficiency. The results were obtained from three complemented clones (−/−/+) and two cultures each of wild type and topo3α mutant.
RAD51-dependent recombination intermediates accumulate in top3 mutants and the removal of persistent intermediates requires the cleavage activity of Top3 , . We examined whether the hyper-switching phenotype of topo3α−/− is dependent on RAD51. Both RAD51 alleles were sequentially deleted in the wild-type and topo3α−/− strains. We analyzed four independent cultures of rad51−/− and two of topo3α−/− rad51−/−. RAD51 deletion reduced the switching frequency of the wild type by 2-fold and abolished the hyper-switching phenotype of topo3α−/− (Figure 5C). Collectively, we concluded that TOPO3α functions as an important regulatory factor for recombination-mediated VSG switching and that, in the absence of TOPO3α, recombinogenic structures may accumulate between the active ES and VSG donors, and could then be resolved to give rise to switched variants.
T. brucei TOPO3α suppresses VSG GC and crossover
In other organisms, Top3 defects are associated with elevated crossover as well as hyper-recombination –, , . To learn how individual switchers had undergone antigenic variation, we analyzed total 296 cloned switchers. The rationales for the double marking of parental cells are as follows (Figure 4). First, switchers can be effectively counter-selected using GCV (Figure 4 and 5). Second, transcription is initiated at silent ESs but elongation is prematurely terminated : genes that are located closer to silent ES promoters are not completely silenced. Therefore, in-situ switchers can be distinguished from recombination-mediated switchers using different concentrations of blasticidin. Based on our titration for blasticidin concentration, in-situ switchers can grow in 5µg/ml blasticidin but not in 100 µg/ml, while ES gene conversion (ES GC) switchers cannot grow in either concentration. VSG gene conversion (VSG GC) and VSG-exchange (crossover) switchers will be resistant to 100 µg/ml blasticidin, and these alternatives can be distinguished by the absence or presence of VSG 427-2, respectively, which can be analyzed by PCR. The strategies to score individual switching mechanisms are summarized in Table 1 and examples of PCR analyses are shown in Figure 6A (right).
(A) TOPO3α suppresses VSG GC and crossover. 296 cloned switchers from three independent cultures of wild type and topo3α−/− were examined. The percentage of each mechanism (VSG GC, crossover, ‘ES GC or ES loss’, in situ, and ES GC+crossover) was plotted. White bars are wild type and dark grey bars are topo3α mutants. PCR results from several switchers are shown in right. TETR region was used as an internal PCR control, as all strains used in this study contain TETR. VSG-GC switchers should be BSD+, VSG 427-2−, and either ESAG1+ or −. ‘ES GC or ES loss’ switchers should be BSD−, VSG 427-2−, and ESAG1−. Crossover should be BSD+, VSG 427-2+, and ESAG1+. In situ should be BSD+, VSG 427-2+, and ESAG1+. The results are also summarized in Table 2. (B) TOPO3α specifically regulates the ES-associated VSG switching. Diagram shows relations between the presence of ES1-specific ESAG1 and the location of recombination that occurred or resolved in VSG-GC switchers. Blue lines under ESAG1 box indicate a region amplified by PCR. Black circles are telomere repeats.
We analyzed cloned switchers isolated from six independent cultures and were able to discriminate among the alternative switching mechanisms. The results are summarized in Table 2 and Figure 6A. Switchers from cultures 1 and 2 were isolated by the column method and switchers from culture 3 by directly plating in GCV. Switching occurred largely by gene conversion (Figure 6A). In both wild type and topo3α mutants, 64~77% of switching exploited VSG GC. Crossovers were rare in wild type (~3%) but, on average, 20% of switchers exchanged their VSGs in topo3α−/−. These data suggest that, in the absence of TOPO3α, recombination intermediates may be accumulated and these could be repaired mostly by duplicative VSG GC and crossover.
In a previous study designed to examine in-situ switching, using a cell line with TK marker inserted next to the active ES promoter, frequent loss of entire active ES was observed. This could be caused by duplicative transposition of a silent ES (ES GC) or by deletion of the active ES coupled with transcriptional activation of a silent ES . In our experiments, ES GC and ES loss cannot be distinguished, as switchers that lost both BSD and VSG 427-2 genes could arise either by duplicative transposition of a silent ES or by ES breakage coupled with an ES transcriptional switch. The ‘ES GC or ES loss’ events were rather frequently detected in wild-type cells (average ~30%), while they were either not detected (culture 1 and 2) or detected at a low frequency (4 our of 51 cloned switchers in culture 3) in the absence of TOPO3α (Figure 6A and Table 2). Interestingly, RAD51 deletion significantly decreased ‘ES GC or ES loss’ frequency (unpublished data), indicating that ‘ES GC or ES loss’ events are mainly under the control of RAD51-dependent recombination.
We noticed that some switched variants had growth disadvantages. Depending on how long it took to saturate the medium, wild-type switchers were categorized as ‘fast’, ‘medium’ or ‘slow’. ‘ES GC or ES loss’ switchers were prevalent in clones that grew up more slowly (data not shown). The functions of ESAGs are mostly unknown, but expressing different ESAGs might be advantageous when entering different hosts . The slower-growth phenotype of some of these switchers may reflect impaired function of one or more ESAGs in the bovine serum-containing culture medium, which appears to favor stable transcription of the VSG 427-2-containing ES1.
In-situ switchers were rare in our assay. This phenotype is different from previous reports , , for reasons we do not understand. In our hands, in-situ switchers generally grew slower than VSG-GC switchers, so VSG-GC switchers would quickly take over the switched population if it was initially mixed, although this is unlikely because our switching population was initiated at 500–1000 cells/ml, while it was at 5,000–10,000 cell/ml in previous assays. Before this seeding, cells were grown in the presence of drugs that prevented switching.
T. brucei TOPO3α specifically regulates ES-associated VSG switching
The 70-bp repeat unit has been proposed to be a recombination hot spot, possibly as a potential target for a site-specific endonuclease playing a similar role to that of the HO-endonuclease in yeast. Such an endonuclease has not been identified in trypanosomes. The 70-bp repeats could serve as switching hot-spots because of their structural features , rather than require cleavage by a specific endonuclease. Early experiments suggested that the overall VSG switching-frequency was not reduced in the absence of 70-bp repeats or by inversion of the repeats although, when present in the correct orientation, the repeats were used more than 10% of the time . More recently, however, it has been shown that the 70-bp repeats of the actively transcribed ES are prone to break, which could induce recombination-mediated switching, and that the switching frequency was greatly increased when breaks were experimentally induced at the 70-bp repeats, but not when induced elsewhere in the ES or in the absence of 70-bp repeats .
We mapped the region where the recombination occurred (or resolved) in the VSG-GC switchers from wild type and topo3α mutants, to learn whether the 70-bp repeat unit is the hot spot of duplicative VSG GC and whether TOPO3α can redirect this preference. ESAG1 genes are located immediately upstream of the 70-bp repeats, and their sequence polymorphisms allowed us to design ES1-specific-ESAG1 oligonucleotides for PCR analysis. PCR results from several VSG-GC switchers were shown in Figure 6A (right). The presence of ES1-specific ESAG1 in VSG-GC switchers indicates that gene conversion occurred at 70-bp repeat regions, and its absence indicates that recombination occurred upstream of ESAG1 (Figure 6B). Crossover and ‘ES GC or ES loss’ switchers were used to verify that the PCR primer set was amplifying only the ES1-specific ESAG1 gene. The ES1-specific ESAG1 was lost in all ‘ES GC or ES loss’ switchers but was detected in all crossover switchers examined, as expected. The ES1-specific ESAG1 gene was amplified in ~63% of VSG-GC switchers in wild-type cells but ~81% of VSG-GC switchers lost the ES1-specific ESAG1 gene in topo3α−/−, indicating that, in the absence of TOPO3α, the active ES recombined mostly with silent ESs upstream of ESAG1, rather than within the 70-bp repeats, but not with minichromosomal or telomere-distal VSGs. We concluded that the 70-bp repeat region is an important but not an essential element for recombination-mediated switching. Gene conversion upstream of 70-bp repeats, at ESAG2, has also been reported . The primary function of TOPO3α may be to prevent accumulation of recombination intermediates constantly arising between the active and silent ESs, to maintain the integrity of ESs.
Recombination by a one-strand invasion event could replace VSGs by break-induced replication (BIR) . Alternatively, a second strand invasion at homologous sequences within or downstream of the VSG could generate VSG-GC switchers. Duplication of a telomere-distal VSG into an active ES is a relatively rare event, at least in the modest extent to which switching events have been characterized experimentally, but it appears to serve as an important switching mechanism in later stage of infection and as a mechanism to further expand the expressed VSG repertoire , , . The few telomere-distal VSG arrays so far characterized contain only short stretches of 70-bp repeats but lack telomeric repeats. To determine how VSG GC occurred, we analyzed the sequences downstream of the 3′ homology region of VSG 427-2 by PCR in all VSG-GC switchers (Supporting Figure S3). If the second strand invaded at this 3′ homology region, downstream sequences should be unchanged. We found, however, that the ES1-specific downstream sequences were lost in all the VSG-GC switchers obtained from wild-type and topo3α cells, indicating that VSG-GC switchers were most likely repaired by BIR, consistent with a recent report , and that internal-VSG duplication is extremely rare. PCR results from a selection of VSG-GC switchers were shown in Figure S3.
To confirm the duplicative translocation of newly expressed VSGs to the VSG 427-2 ES and to examine whether minichromosomal VSGs contribute to antigenic switching, 32 VSG-GC switchers from wild-type cells were further analyzed. Minichromosomes terminate with telomeres, VSGs and 70-bp repeats. Gene conversion with minichromosomal VSGs occurs frequently , but only when recombination is initiated at the 70-bp repeats. Therefore, we cloned and sequenced newly activated VSGs from VSG-GC switchers that utilized 70-bp repeats. From 32 switchers that had undergone at least one type of switching, VSG GC at the 70-bp repeats, we obtained eight different newly activated VSGs (Supporting Figure S5, left). It is possible that we have underestimated the number of independent switching events as these switchers may have used different sequences within or near the 70-bp repeats, which should be counted as independent. Some switchers might have arisen earlier than others, for examples VSG 427-32, as these were presented more often than others. Among these eight newly expressed VSGs, four were novel VSGs, 427-32, 33, 34 and 35, full or partial sequences of which can be found in the following website (http://tryps.rockefeller.edu). Switchers expressing VSGs 427-3, 11, 32, 33 and 35 were examined by rotating agarose gel electrophoresis (RAGE) and Southern blot . As shown in Supporting Figure S5 (right panel), VSG 427-2 was lost in all the switchers and all newly expressed VSGs were duplicated and translocated to the 427-2 ES, except for 427-33, an intermediate chromosomal (IC) VSG. The original copy of 427-33 may be lost after recombination. VSGs 427-32 and 35 came from megabase chromosomes (MBC). We have not isolated any minichromosomal VSGs in these switchers, indicating that recombination between ES-associated VSGs was the major source for VSG switching.
Repair by recombination serves to preserve genome integrity and can either homogenize or diversify genetic information, occasionally causing detrimental outcomes or benefiting certain organisms by providing adaptation systems to escape lethal situations. African trypanosomes escape the host immune response through a mechanism known as the antigenic variation. Here, we report that T. brucei TOPO3α, a member of a potential T. brucei RecQ-Top3-Rmi1 (RTR) complex, takes an important part in the regulation of recombination-mediated antigenic variation. Our results reveal a complex mechanism that has to balance ES integrity and VSG diversity to maximize the survival of a trypanosome population by suppressing crossovers on one hand and by promoting duplicative VSG gene conversions on the other.
Mechanism of recombination-mediated antigenic switching and roles for TOPO3α
As illustrated in Figure 7, ES structures seem to play a particular role in VSG switching. ES-associated VSG genes are located between the 70-bp and telomeric repeats. ESAGs and some pseudogenes are present upstream of the 70-bp repeats in all ESs, sometimes duplicated and sometimes missing , . Strong sequence homologies are present throughout the ESs, with the exception of most of the VSG coding sequence and the immediately upstream ‘co-transposed region’ (CTR). VSG sequences are highly dissimilar except for ~200-bp encoding the C-terminus and within the 3′ UTR . The reason why every VSG cassette contains a unique CTR is unknown. The purpose of CTR could be to insulate the individuality of VSG cassettes, so that the VSG sequences can evolve separately from other regions in ESs, which maintain their sequences to serve for VSG recombination. When HR occurs, the CTR could block branch migration of HJ or dHJ downstream of the 70-bp repeats.
The diagram shows the active ES (ES1) expressing VSG 427-2 and a silent ES containing vsg X. VSG genes are located between 70-bp repeats and telomere repeats (black circles). The sequence of each VSG cassette, including CTR, VSG, and VSG downstream, is unique (red for VSG 427-2 and green for vsgX). The strong sequence similarities are present throughout these ESs. Holliday Junctions (HJs) or double HJs (dHJs) can form between these ESs but cannot migrate downstream of the 70-bp repeats. These intermediates have to be resolved before CTR or have to use telomere repeats or sequence homology within VSG cassettes to generate switched variants. The grey boxes (in (a) and (b)) include potential players that could generate switched variants in the absence of TOPO3α. (a) The dHJs can be efficiently processed by TOPO3α, generating non-crossover (no switching). However, in the absence of TOPO3α, HJ can be cleaved by resolvase (brown scissors) to generate non-crossover (no switching) and crossover products (crossover switchers). (b) Replication fork instability can accumulate recombination intermediates between sister-chromatids. In the absence of TOPO3α, these intermediates can be cleaved by MUS81, a 3′ flap endonuclease (blue triangle), and the broken leading strand can invade sister-chromatid to complete replication. Alternatively this can also invade a silent ES using their sequence homology and replicate to the end of the chromosome, generating VSG GC switchers.
What roles does TOPO3α play in this scheme? Our study shows that TOPO3α deficiency increases VSG switching, especially VSG GC and crossover, and that the hyper-switching phenotype requires RAD51. The accumulation of toxic recombination intermediates accounts for the slow growth phenotype of yeast top3 mutants, which is suppressed by mutations in SGS1 or in the RAD51-pathway , , . Recombination intermediates accumulate in cells over-expressing dominant-negative Top3-Y356F in response to methylmethane sulfonate in a RAD51-dependent manner . The function of TOPO3α is not restricted to the 70-bp repeats in antigenic switching, as its absence appears to cause promiscuous recombination throughout the ESs. We therefore propose that TOPO3α removes recombinogenic structures constantly arising between ESs so as to maintain the albeit limited individuality of different ESs. In the absence of TOPO3α, recombination intermediates would accumulate during VSG switching and unresolved intermediates would have to be repaired either by GC associated with crossover or by placing a new duplicated VSG into the active ES by BIR (Figure 7).
Suppression of crossover in recombination-mediated VSG switching is an interesting result, considering that there are probably more than 200 potential VSG donors: ~20 ESs with extensive sequence homology and ~200 minichromosomal VSGs. Antigenic variation probably requires balancing preservation and variation of VSG information, but we cannot explain how suppression of crossover would be important for maintaining this balance. However, we think that by favoring duplicative GC over crossover, rather than crossover over GC, trypanosomes could slowly accumulate VSG diversity without abrupt loss of their functionalities, because duplicative GC requires VSG DNA synthesis, during which point mutations could be incorporated into newly synthesized VSGs, but VSG crossover does not require VSG DNA synthesis.
TOPO3α deficiency increased VSG GC far more than GC at the URA3 locus (Figures 3 and 6). GC at these two loci is probably mediated by different pathways. Recombination at URA3 locus would prefer flanking homologies, rather than BIR. In contrast, BIR would present a better option for VSG GC, as only one end homology appears to be involved (supporting Figure S3) . It is possible that a second invasion could occur within the telomere repeats, but this is impossible to determine. The higher VSG GC rate could also be because the active ES is less stable than URA3 locus. Alternatively, TOPO3α may specifically suppress BIR-mediated VSG switching. The role of TOPO3α in BIR has not been extensively characterized elsewhere. Our results show a novel function of TOPO3α in VSG switching, which could be an excellent system to study BIR.
TbRTR complex and DSB-HR response in antigenic variation
DNA recombination involves many factors, of which only a few have been studied in the context of antigenic variation: RAD51, RAD51-related genes, BRCA2, KU70/80, MRE11, and MSH2/MLH1 –, , , , . Among these, only the deletion of RAD51, RAD51-3, and BRCA2 decreased VSG switching, in wild-type cells that already had a very low switching rate.
Our findings on TOPO3α in VSG switching suggest potential roles for numerous DSB-HR response factors in antigenic variation. Two RecQ family helicases are annotated in the T. brucei gene database (http://www.genedb.org/genedb/tryp). Rmi1 is required to load Top3 onto the substrates and stimulate its activity through the physical interaction . We have identified a TbRMI1 homologue. All the phenotypes that we have examined in Tbrmi1 mutants were identical to those in topo3α mutants (unpublished data). Therefore, we believe that RecQ, TOPO3α and RMI1 are likely to function as a complex in antigenic variation in T. brucei.
Synthetic-lethality screens with sgs1 in budding yeast identified three pathways working in parallel with Sgs1 ; Mus81-Mms4, Slx1-Slx4, and Slx5-Slx8. Synthetic lethality of sgs1 mus81 or sgs1 mms4 requires HR factors . Mus81-Mms4 is a structure-specific endonuclease that cleaves 3′ flap, replication fork, or HJ substrates –. Resolvase, an endonuclease, symmetrically cleaves HJs and the products can be resolved with crossover or non-crossover. Human and yeast resolvases have recently been characterized . MUS81 appears to be present in T. brucei but a resolvase remains to be identified. Although we do not yet have functional data for these proteins, we propose, based on the studies from other organisms, that the regulation of antigenic variation is similar to that of mitotic HR. When present, TOPO3α could dissolve dHJs to prevent the ES instability, consequently generating non-crossover recombinants (no switching). In the absence of TOPO3α, resolvase (Figure 7a, grey box) or MUS81 may cleave the accumulated recombination intermediates arising between the ESs and generate crossover switchers. Alternatively, stalled replication forks can be cleaved by MUS81 and the broken leading strand can invade a silent ES to generate VSG-GC switchers (Figure 7b, grey box).
Although VSG switching has similarities with mitotic HR, it appears that specific elements are present for its regulation. A hyper-recombination phenotype does not always correlate with hyper-switching phenotype. The mismatch repair (MMR) pathway can abort recombination during strand exchange between non-identical substrates and mmr mutants can increase recombination frequency (reviewed in ). Consistent with their roles in repair and recombination, Tbmsh2 or Tbmlh1 mutants increased recombination frequency but did not change switching frequency . Recombination is closely linked with DNA replication and checkpoint pathways as well , , , . Therefore, we believe that roles for DNA replication, checkpoint, and recombination factors and their interactions need to be determined to fully understand the mechanisms of antigenic variation.
Measuring VSG switching has, until now, been time-consuming and not very reproducible. Our new switching assay circumvents previous technical difficulties and can effectively assign specific roles to individual proteins.
What triggers antigenic switching
It has recently been shown that a DSB introduced at the active 70-bp repeats by the I-SceI endonuclease causes a 250-fold increase in VSG switching and that the DSBs were repaired by BIR . However, it is unknown whether the VSG switching is activated by targeted DSBs or by random chromosomal breaks, or whether recombinogenic ssDNA is a primary cause for the initiation of VSG switching. HR can be instigated by many different sources; random breaks, endonuclease cleavage at specific target sites, replication fork instability, unusual secondary DNA structure, or transcription.
The Mre11 complex, which consists of Mre11, Rad50, and Xrs2 (NBS1 in mammals), plays a central role in the DSB-HR response –. MRE11 deficiency, however, did not change the VSG switching frequency , , promoting the idea that ssDNA regions may generate recombinogenic structures for the initiation of switching. Uncoupling of leading and lagging strand DNA synthesis caused by DNA lesions can destabilize a replication fork, leaving ssDNA gaps behind the fork, which could be processed into recombinogenic structures. If an ssDNA gap is a major trigger for recombination-mediated switching, switching frequency should increase in cells suffering from replication challenge. To address this issue, we treated cells with aphidicolin, an inhibitor of lagging strand DNA synthesis, and HU, and measured the switching frequency in parallel (Supporting Figure S4). Cells were treated with the drugs at a sub-lethal dose to exclude a possibility of chromosome break-induced switching. No significant correlation was observed between these treatments and switching frequency. Therefore, an ssDNA gap may not be a major initiating factor for VSG switching. Rather, random breaks might be responsible for switching induction, consistent to a previous study . However, it is still difficult to rule out the possibility that an ssDNA gap triggers switching, as ssDNA gaps might not be extensive enough to create recombinogenic structures at the low doses of aphidicolin or HU. The best way to test this hypothesis would be to use conditional mutants associated with replication defects. Unfortunately, we do not yet have such genetic tools, as nuclear DNA replication has not been studied in T. brucei.
A high transcription level can stimulate recombination, a mechanism known as transcription-associated recombination (TAR) (reviewed in ). Transcription has been shown to promote recombination in T. brucei , . Interestingly, it was shown in budding yeast that transcription- and DSB-induced recombination events were similar, indicating that transcription affects only the initiation of recombination, not the mechanism of recombination . ssDNA regions exposed in the active ES during transcription could be readily accessible by recombination factors. Alternatively, transcription-replication collision causes replication fork stalling, which could also induce switching. Studies of mammalian cells have shown that TAR is dependent on replication , and that transcription increases recombination frequency when a replication fork converges with transcription . The active ES is more fragile than silent ESs . The high level of transcription may explain why the active ES breaks more frequently, and this may induce VSG switching.
The 70-bp repeat has been proposed to be a potential endonuclease target site to induce switching, but such an enzyme has not been found. Instability of the 70-bp repeat  may play a role in the initiation of switching and could lead to template switching. However, according to our results and previous studies , , switching is not completely dependent on the 70-bp repeats. With the available data, it would be reasonable to conclude that random breaks may occur throughout the active ES but more frequently at 70-bp repeats, and these could initiate various switching events.
Gene conversion is used by several other pathogens, including Borrelia hermsii and Anaplasma marginale, as an evasion mechanism , . Our study suggests that exploring how trypanosomes manipulate the HR machinery to gain advantage against their host's immunity, while successfully preserving their genomes, may reveal weaknesses that can be exploited to control infectivity and virulence.
Materials and Methods
Trypanosome strains and plasmids
Trypanosoma brucei bloodstream forms (strain Lister 427 antigenic type MITat1.2 clone 221a (VSG 427-2)) were cultured in HMI-9 at 37°C. The cell lines constructed for this study are listed in Supporting Table S1, and they are of ‘single marker’ (SM) background that expresses T7 RNA polymerase and Tet repressor (TETR) . Stable clones were obtained and maintained in HMI-9 media containing necessary antibiotics at the following concentrations, unless otherwise stated: 2.5µg/ml, G418 (Sigma); 5µg/ml, blasticidin (Invivogen); 5µg/ml, hygromycin (Sigma); 0.1µg/ml, puromycin (Sigma); 1µg/ml, phleomycin (Invivogen). Plasmids used for this study are listed in Supporting Table S2.
Construction of topo3α−/− cell line and removal of markers using Cre recombinase-loxPs (Supporting Figure S2)
TOPO3α genes were sequentially deleted using deletion-cassettes containing either puromycin or hygromycin-resistance gene fused with HSVTK, Herpes simplex virus thymidine kinase (TK), PUR-TK and HYG-TK. These fusion genes are flanked by loxP sites so that the markers can be removed by transient expression of Cre recombinase (pLew100-Cre). The entire open reading frame (ORF) of TOPO3α was deleted by transfecting ‘single marker’ (SM) cells with a deletion-cassette that was amplified with primer 35 and 36 using pHJ18 (PUR-TK) as a template. Primer 35 and 36 contains 70 nt homologies to the target sites. This topo3α ‘single knock-out’ cells (sKO, HSTB-97) were used to PCR amplify a cassette containing a marker (PUR-TK) along with 453 nt upstream and 402 nt downstream sequences of TOPO3α gene. The PCR fragment was inserted into pGEM-easy-T vector by TA cloning to create pHJ63. pHJ64 was constructed by replacing a PUR-TK marker with a HYG-TK from pHJ17. topo3α ‘double knock-out’ (dKO) was generated by transfecting NotI-digested pHJ64 into topo3α sKO, HSTB-97. Deletion of both TOPO3α alleles was confirmed by PCR analyses.
To remove the selection markers, topo3α dKO cells were transfected with pLew100-Cre to transiently express Cre-recombinase, and the cells that lost both HYG-TK and PUR-TK were selected in 50µg/ml ganciclovir (GCV). Loss of markers was confirmed by resistance to puromycin and hygromycin, and by PCR analysis. The sequences of primers used here are available upon request.
pLHTL-pyrFE -linearized by PvuII digestion was transfected into wild-type (HSTB-188) and topo3α−/− (HSTB-328 and HSTB-330) cells, to replace one allele of TbURA3 with HYG-TK. The integration was confirmed by PCR analysis with primers 48 and 49. Three or five independent HYGR clones from wild-type or topo3−/− cells were analyzed. Cells were grown in the absence of hygromycin for 2 days to allow recombination to occur. Approximately 500,000 cells were diluted in HMI-9 media containing 30 µg/ml GCV or 6 µg/ml FOA, and distributed into 96-well plates. Yellow wells (phenol red indicating acidification due to growth) containing GCVR or FOAR cells were counted after 7–8 days of incubation and the GC frequency was determined. The sequences of primers used for genotyping are available upon request.
Switching assay and analyses of switchers
To create a doubly-marked switching reporter strain (Figure 4), pHJ23 was linearized by KpnI-NotI digestion and integrated downstream of the ES1 promoter, to confer resistance to blasticidin. These cells were then marked with PUR-TK at the 3′ end of 70-bp repeats by transfecting a PCR-amplified PUR-TK cassette. Ten µg/ml of puromycin, 100 times higher than normal usage, was added to select clones targeted specifically at the active ES. When determining switching frequency, the parental cells were maintained in the presence of blasticidin and puromycin to exclude switchers from the starting population. Cells were then allowed to switch in the absence of selection for 3–4 days. Switchers were enriched using a MACS . Flow-through enriched with switchers was collected and serially diluted in media containing 4 µg/ml GCV, and distributed into 96-well plates. The switching frequency was determined by counting GCVR clones. Alternatively, switching frequency was determined without the column enrichment step. Cells were diluted in GCV-containing media and directly distributed into 96-well plates. Non-switchers that carry spontaneous mutation(s) in TK gene but not in PUR were ruled out by examining puromycin resistance. Non-switchers that carry mutations in PUR and TK were ruled out by western blot analysis using antibodies against VSG 427-2.
To determine switching mechanisms, cloned switchers were analyzed for blasticidin sensitivity at 5 µg/ml and 100 µg/ml concentrations. Genomic DNA was prepared from 296 switchers and PCR-analyses were performed at four regions: BSD, VSG 427-2, ESAG1, and VSG 427-2 downstream. The primer set designed for BSD-PCR can also amplify TETR (Tet Repressor) gene, which was used as a control for PCR analyses. The sequences of primers used here are available upon request.
Analysis of sensitivity to genotoxic agents
Wild type (SM), topo3α−/+ (HSTB-97), and topo3α−/− (HSTB-226 and HSTB-227) cells were incubated with indicated concentration of phleomycin for 24 hours. The same number of cells was distributed into 96-well plates. All the plating was duplicated. The wells that contain viable cells were counted after 7–8 days of incubation at 37°C and the viability was calculated by normalizing to untreated samples. Sensitivity to HU and aphidicolin was determined similarly. Cells were incubated with HU or aphidicolin for 2 or 3 days. The viability was calculated by normalizing to untreated samples.
Gene accession numbers
Database ID numbers (http://www.genedb.org and http://tritrypdb.org) for TOPO3α discussed in this paper are Tb11.01.1280, LmjF36.3200 and Tc00.1047053511589.120. What we refer to as TbURA3 is the bifunctional orotidine-5-phosphate decarboxylase/orotate phosphoribosyltransferase Tb927.5.3810.
Strains used in this study
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Plasmids used in this study
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Alignment of T. brucei, T. cruzi and L. major TOPO3α. The colored boxes indicate domains found in SMART (Simple Modular Architecture Research Tool) domain search: yellow box, TOPRIM (topoisomerase-primase) domain; purple, TOP1Bc (bacterial DNA topoisomerase I ATP-binding domain); red, TOP1Ac (bacterial DNA topoisomerase I DNA binding domain); green, Zf-C4 (zinc-finger domain). The catalytic tyrosine (Y) is written in yellow. Four cysteine residues are written in red in green box. Gene numbers are Tb11.01.1280, LmjF36.3200 and Tc00.1047053511589.120.
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Construction of topo3α−/− cell line and removal of markers using Cre recombinase-loxPs. TOPO3α genes were sequentially deleted using deletion-cassettes containing PUR-TK and HYG-TK. These fusion genes are flanked by loxP sites so that the markers can be removed by transient expression of Cre recombinase (pLew100-Cre) .
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Switching occurred by BIR in VSG-GC switchers. Diagram shows two scenarios of how new VSG can be duplicated and placed in the active ES. The first strand invasion should occur upstream of CTR and this can replicate all the way to the end of the chromosome or recombine a second time at the homology regions present at the C-terminus or in the 3′ UTR of VSG 427-2 (221) (3′ homology region). To distinguish these possibilities, the downstream region specific for VSG 427-2 (221) was analyzed in all the switchers by PCR. 12 switchers (2 crossover and 10 VSG-GC switchers) are shown as representatives. Black circles are telomere repeats. Blue lines next to telomere repeats indicate a region analyzed by PCR.
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Switching frequency was not affected by aphidicolin or HU treatments. (A) Wild-type cells were treated with the indicated concentrations of aphidicolin and percent viability was determined by normalizing to the untreated sample. (B) Switching frequency of aphidicolin-treated cells was measured in parallel, by directly plating in GCV-containing media without enrichment. (C) Wild-type cells were treated with 0.01 mM HU, and topo3α cells were treated with 0.01 mM HU or 1 ng/ml aphidicolin (APH) for 3 days. Percent viability was determined by normalizing to untreated sample. (D) Switching frequency of HU or aphidicolin-treated wild-type or mutant cells was measured in parallel by the column-enrichment method.
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Cloning newly activated VSGs. Total mRNA was extracted from VSG-GC switchers that utilized 70-bp repeats for VSG recombination. cDNA was amplified using a reverse-transcriptase and oligo dT20 (Stratagene). Newly expressed VSGs were amplified using specific oligos that anneal to the spliced leader and to 16-mer sequences present in all VSG transcripts, and sequenced. Eleven switchers expressing 427-3 (224), 427-11 (bR2), 427-32, 427-33 or 427-35 were further analyzed to confirm duplicative translocation of new VSGs to the VSG 427-2 expression site by rotating agarose gel electrophoresis (RAGE) and Southern blotting , using probes specific to VSGs 427-2 (221), 427-3, 427-11, 427-32, 427-33, or 427-35. Abbreviations: MBC (megabase chromosome), IC (intermediate chromosome), MC (minichromosome), and P (parental strain expressing VSG 427-2). Arrowheads indicate translocation of newly activated VSGs to ES1.
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We thank Nina Papavasiliou and Steven Brill for comments on the manuscript. We also thank members of Cross lab, especially Jenny Li for technical help.
Conceived and designed the experiments: HSK GAMC. Performed the experiments: HSK. Analyzed the data: HSK GAMC. Wrote the paper: HSK GAMC.
- 1. Marcello L, Barry JD (2007) Analysis of the VSG gene silent archive in Trypanosoma brucei reveals that mosaic gene expression is prominent in antigenic variation and is favored by archive substructure. Genome Res 17: 1344–1352.
- 2. Marcello L, Menon S, Ward P, Wilkes JM, Jones NG, et al. (2007) VSGdb: a database for trypanosome variant surface glycoproteins, a large and diverse family of coiled coil proteins. BMC Bioinformatics 8: 143.
- 3. Horn D, Barry JD (2005) The central roles of telomeres and subtelomeres in antigenic variation in African trypanosomes. Chrom Res 13: 525–533.
- 4. Barry JD, McCulloch R (2001) Antigenic variation in trypanosomes: enhanced phenotypic variation in a eukaryotic parasite. In: Baker JR, Muller R, Rollinson D, editors. Advances in Parasitology 49. London: Academic Press Ltd. pp. 1–70.
- 5. Cross GAM (2002) Antigenic variation in african trypanosomes and malaria. In: Marr J, Koumuniecki R, Nilsen TW, editors. Mol Med Parasitol. Academic Press. pp. 89–110.
- 6. Rudenko G, Bishop D, Gottesdiener K, van der Ploeg LHT (1989) Alpha-amanitin resistant transcription of protein coding genes in insect and bloodstream form Trypanosoma brucei. EMBO J 8: 4259–4263.
- 7. Palenchar J, Bellofatto V (2006) Gene transcription in trypanosomes. Mol Biochem Parasitol 146: 135–141.
- 8. Gunzl A, Bruderer T, Laufer G, Schimanski B, Tu LC, et al. (2003) RNA polymerase I transcribes procyclin genes and variant surface glycoprotein gene expression sites in Trypanosoma brucei. Eukaryot Cell 2: 542–551.
- 9. Hertz-Fowler C, Figueiredo LM, Quail MA, Becker M, Jackson A, et al. (2008) Telomeric expression sites are highly conserved in Trypanosoma brucei. PLoS ONE 3: e3527.
- 10. Machado C, Augusto-Pinto L, McCulloch R, Teixeira S (2006) DNA metabolism and genetic diversity in Trypanosomes. Mutat Res Rev-Mutat Res 612: 40–57.
- 11. Zomerdijk JCBM, Ouellete M, ten Asbroek ALMA, Kieft R, Bommer AMM, et al. (1990) The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei. EMBO J 9: 2791–2801.
- 12. Johnson PJ, Borst P (1986) Mapping of VSG genes on large expression-site chromosomes of Trypanosoma brucei separated by pulse-field gradient electrophoresis. Gene 43: 213–220.
- 13. Pays E, van Assel S, Laurent M, Darville M, Vervoort T, et al. (1983) Gene conversion as a mechanism for antigenic variation in trypanosomes. Cell 34: 371–381.
- 14. Myler P, Nelson RG, Agabian N, Stuart K (1984) Two mechanisms of expression of a variant antigen gene of Trypanosoma brucei. Nature 309: 282–284.
- 15. Lee MG-S, van der Ploeg LHT (1987) Frequent independent duplicative transpositions activate a single VSG gene. Mol Cell Biol 7: 357–364.
- 16. de Lange T, Kooter JM, Michels PAM, Borst P (1983) Telomere conversion in trypanosomes. Nucl Acids Res 11: 8149–8165.
- 17. Robinson NP, Burman N, Melville SE, Barry JD (1999) Predominance of duplicative VSG gene conversion in antigenic variation in African trypanosomes. Mol Cell Biol 19: 5839–5846.
- 18. Hoeijmakers JHJ, Frasch ACC, Bernards A, Borst P, Cross GAM (1980) Novel expression-linked copies of the genes for variant surface antigens in trypanosomes. Nature 284: 78–80.
- 19. Pays E, Guyaux M, Aerts D, vanMeirvenne N, Steinert M (1985) Telomeric reciprocal recombination as a possible mechanism for antigenic variation in trypanosomes. Nature 316: 562–564.
- 20. Aitcheson N, Talbot S, Shapiro J, Hughes K, Adkin C, et al. (2005) VSG switching in Trypanosoma brucei: antigenic variation analysed using RNAi in the absence of immune selection. Mol Microbiol 57: 1608–1622.
- 21. Rudenko G, McCulloch R, Dirksmulder A, Borst P (1996) Telomere exchange can be an important mechanism of variant surface glycoprotein gene switching in Trypanosoma brucei. Mol Biochem Parasitol 80: 65–75.
- 22. Bernards A, van der Ploeg LHT, Gibson WC, Leegwater P, Eijgenraam F, et al. (1986) Rapid change of the repertoire of variant surface glycoprotein genes in trypanosomes by gene duplication and deletion. J Mol Biol 190: 1–10.
- 23. Proudfoot C, McCulloch R (2005) Distinct roles for two RAD51-related genes in Trypanosoma brucei antigenic variation. Nucl Acids Res 33: 6906–6919.
- 24. McCulloch R, Barry JD (1999) A role for RAD51 and homologous recombination in Trypanosoma brucei antigenic variation. Genes Dev 13: 2875–2888.
- 25. Hartley CL, McCulloch R (2008) Trypanosoma brucei BRCA2 acts in antigenic variation and has undergone a recent expansion in BRC repeat number that is important during homologous recombination. Mol Microbiol 68: 1237–1251.
- 26. D'Amours D, Jackson SP (2002) The Mre11 complex: at the crossroads of DNA repair and checkpoint signalling. Nat Rev Mol Cell Biol 3: 317–327.
- 27. Williams RS, Moncalian G, Williams JS, Yamada Y, Limbo O, et al. (2008) Mre11 dimers coordinate DNA end bridging and nuclease processing in double-strand-break repair. Cell 135: 97–109.
- 28. Kanaar R, Wyman C (2008) DNA repair by the MRN complex: break it to make it. Cell 135: 14–16.
- 29. Tan KSW, Leal SLG, Cross GAM (2002) Trypanosoma brucei MRE11 is non-essential but influences growth, homologous recombination and DNA double-strand break repair. Mol Biochem Parasitol 125: 11–21.
- 30. Robinson NP, McCulloch R, Conway C, Browitt A, Barry JD (2002) Inactivation of Mre11 does not affect VSG gene duplication mediated by homologous recombination in Trypanosoma brucei. J Biol Chem 277: 26185–26193.
- 31. Ira G, Malkova A, Liberi G, Foiani M, Haber JE (2003) Srs2 and Sgs1-Top3 suppress crossovers during double-strand break repair in yeast. Cell 115: 401–411.
- 32. Bachrati CZ, Hickson ID (2008) RecQ helicases: guardian angels of the DNA replication fork. Chromosoma 117: 219–233.
- 33. Seki M, Tada S, Enomoto T (2006) Function of recQ family helicase in genome stability. Subcell Biochem 40: 49–73.
- 34. Sung P, Klein H (2006) Mechanism of homologous recombination: mediators and helicases take on regulatory functions. Nat Rev Mol Cell Biol 7: 739–750.
- 35. Chang M, Bellaoui M, Zhang C, Desai R, Morozov P, et al. (2005) RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex. EMBO J 24: 2024–2033.
- 36. Mullen JR, Nallaseth FS, Lan YQ, Slagle CE, Brill SJ (2005) Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Mol Cell Biol 25: 4476–4487.
- 37. Tsai HJ, Huang WH, Li TK, Tsai YL, Wu KJ, et al. (2006) Involvement of topoisomerase III in telomere-telomere recombination. J Biol Chem 281: 13717–13723.
- 38. Huang PH, Pryde FE, Lester D, Maddison RL, Borts RH, et al. (2001) SGS1 is required for telomere elongation in the absence of telomerase. Curr Biol 11: 125–129.
- 39. Neff NF, Ellis NA, Ye TZ, Noonan J, Huang K, et al. (1999) The DNA helicase activity of BLM is necessary for the correction of the genomic instability of bloom syndrome cells. Mol Biol Cell 10: 665–676.
- 40. Singh TR, Ali AM, Busygina V, Raynard S, Fan Q, et al. (2008) BLAP18/RMI2, a novel OB-fold-containing protein, is an essential component of the Bloom helicase-double Holliday junction dissolvasome. Genes Dev 22: 2856–2868.
- 41. Xu D, Guo R, Sobeck A, Bachrati CZ, Yang J, et al. (2008) RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability. Genes Dev 22: 2843–2855.
- 42. Watt PM, Hickson ID, Borts RH, Louis EJ (1996) SGS1, a homologue of the Bloom's and Werner's syndrome genes, is required for maintenance of genome stability in Saccharomyces cerevisiae. Genetics 144: 935–945.
- 43. Wallis JW, Chrebet G, Brodsky G, Rolfe M, Rothstein R (1989) A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase. Cell 58: 409–419.
- 44. Wu L, Hickson ID (2003) The Bloom's syndrome helicase suppresses crossing over during homologous recombination. Nature 426: 870–874.
- 45. Hartung F, Suer S, Knoll A, Wurz-Wildersinn R, Puchta H (2008) Topoisomerase 3alpha and RMI1 suppress somatic crossovers and are essential for resolution of meiotic recombination intermediates in Arabidopsis thaliana. PLoS Genet 4: e1000285.
- 46. Seki M, Nakagawa T, Seki T, Kato G, Tada S, et al. (2006) Bloom helicase and DNA topoisomerase IIIalpha are involved in the dissolution of sister chromatids. Mol Cell Biol 26: 6299–6307.
- 47. Champoux JJ (2001) DNA topoisomerases: structure, function, and mechanism. Annu Rev Biochem 70: 369–413.
- 48. Scahill MD, Pastar I, Cross GA (2008) CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei. Mol Biochem Parasitol 157: 73–82.
- 49. Maftahi M, Han CS, Langston LD, Hope JC, Zigouras N, et al. (1999) The top3(+) gene is essential in Schizosaccharomyces pombe and the lethality associated with its loss is caused by Rad12 helicase activity. Nucl Acids Res 27: 4715–4724.
- 50. Li W, Wang JC (1998) Mammalian DNA topoisomerase IIIalpha is essential in early embryogenesis. Proc Natl Acad Sci U S A 95: 1010–1013.
- 51. Kwan KY, Wang JC (2001) Mice lacking DNA topoisomerase IIIbeta develop to maturity but show a reduced mean lifespan. Proc Natl Acad Sci U S A 98: 5717–5721.
- 52. Oakley TJ, Goodwin A, Chakraverty RK, Hickson ID (2002) Inactivation of homologous recombination suppresses defects in topoisomerase III-deficient mutants. DNA repair 1: 463–482.
- 53. Oh M, Choi IS, Park SD (2002) Topoisomerase III is required for accurate DNA replication and chromosome segregation in Schizosaccharomyces pombe. Nucl Acids Res 30: 4022–4031.
- 54. Chakraverty RK, Kearsey JM, Oakley TJ, Grenon M, de La Torre Ruiz MA, et al. (2001) Topoisomerase III acts upstream of Rad53p in the S-phase DNA damage checkpoint. Mol Cell Biol 21: 7150–7162.
- 55. Davies KP, Carruthers VB, Cross GAM (1997) Manipulation of the VSG co-transposed region increases expression-site switching in Trypanosoma brucei. Mol Biochem Parasitol 86: 163–177.
- 56. Boothroyd CE, Dreesen O, Leonova T, Ly KI, Figueiredo LM, et al. (2009) A yeast-endonuclease-generated DNA break induces antigenic switching in Trypanosoma brucei. Nature 459: 278–281.
- 57. Liberi G, Maffioletti G, Lucca C, Chiolo I, Baryshnikova A, et al. (2005) Rad51-dependent DNA structures accumulate at damaged replication forks in sgs1 mutants defective in the yeast ortholog of BLM RecQ helicase. Genes Dev 19: 339–350.
- 58. Mankouri HW, Hickson ID (2006) Top3 processes recombination intermediates and modulates checkpoint activity after DNA damage. Mol Biol Cell 17: 4473–4483.
- 59. Vanhamme L, Poelvoorde P, Pays A, Tebabi P, Xong HV, et al. (2000) Differential RNA elongation controls the variant surface glycoprotein gene expression sites of Trypanosoma brucei. Mol Microbiol 36: 328–340.
- 60. Cross M, Taylor MC, Borst P (1998) Frequent loss of the active site during variant surface glycoprotein expression site switching in vitro in Trypanosoma brucei. Mol Cell Biol 18: 198–205.
- 61. Bitter W, Gerrits H, Kieft R, Borst P (1998) The role of transferrin-receptor variation in the host range of Trypanosoma brucei. Nature 391: 499–502.
- 62. McCulloch R, Rudenko G, Borst P (1997) Gene conversions mediating antigenic variation in Trypanosoma brucei can occur in variant surface glycoprotein expression sites lacking 70-base-pair repeat sequences. Mol Cell Biol 17: 833–843.
- 63. Ohshima K, Kang S, Larson JE, Wells RD (1996) TTA.TAA triplet repeats in plasmids form a non-H bonded structure. J Biol Chem 271: 16784–16791.
- 64. Kooter JM, Winter AJ, de Oliveira C, Wagter R, Borst P (1988) Boundaries of telomere conversion in Trypanosoma brucei. Gene 69: 1–11.
- 65. Liu AY, Michels PA, Bernards A, Borst P (1985) Trypanosome variant surface glycoprotein genes expressed early in infection. J Mol Biol 175: 383–386.
- 66. Timmers HTM, de Lange T, Kooter JM, Borst P (1987) Coincident multiple activations of the same surface antigen gene in Trypanosoma brucei. J Mol Biol 184: 81–90.
- 67. Aline RF, Stuart KD (1989) Trypanosoma brucei: conserved sequence organisation 3′ to telomeric variant surface glycoprotein genes. Exp Parasitol 68: 57–66.
- 68. Gangloff S, McDonald JP, Bendixen C, Arthur L, Rothstein R (1994) The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase. Mol Cell Biol 14: 8391–8398.
- 69. Shor E, Gangloff S, Wagner M, Weinstein J, Price G, et al. (2002) Mutations in homologous recombination genes rescue top3 slow growth in Saccharomyces cerevisiae. Genetics 162: 647–662.
- 70. Conway C, McCulloch R, Ginger ML, Robinson NP, Browitt A, et al. (2002) Ku is important for telomere maintenance, but not for differential expression of telomeric VSG genes, in African trypanosomes. J Biol Chem 277: 21269–21277.
- 71. Bell JS, McCulloch R (2003) Mismatch repair regulates homologous recombination, but has little influence on antigenic variation, in Trypanosoma brucei. J Biol Chem 278: 45182–45188.
- 72. Chen CF, Brill SJ (2007) Binding and activation of DNA topoisomerase III by the Rmi1 subunit. J Biol Chem 282: 28971–28979.
- 73. Mullen JR, Kaliraman V, Ibrahim SS, Brill SJ (2001) Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae. Genetics 157: 103–118.
- 74. Bastin-Shanower SA, Fricke WM, Mullen JR, Brill SJ (2003) The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10. Mol Cell Biol 23: 3487–3496.
- 75. Kaliraman V, Mullen JR, Fricke WM, Bastin-Shanower SA, Brill SJ (2001) Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease. Genes Dev 15: 2730–2740.
- 76. Cote AG, Lewis SM (2008) Mus81-dependent double-strand DNA breaks at in vivo-generated cruciform structures in S. cerevisiae. Mol Cell 31: 800–812.
- 77. Ip SC, Rass U, Blanco MG, Flynn HR, Skehel JM, et al. (2008) Identification of Holliday junction resolvases from humans and yeast. Nature 456: 357–361.
- 78. Surtees JA, Argueso JL, Alani E (2004) Mismatch repair proteins: key regulators of genetic recombination. Cytogenet Genome Res 107: 146–159.
- 79. Cobb JA, Schleker T, Rojas V, Bjergbaek L, Tercero JA, et al. (2005) Replisome instability, fork collapse, and gross chromosomal rearrangements arise synergistically from Mec1 kinase and RecQ helicase mutations. Genes Dev 19: 3055–3069.
- 80. Gottipati P, Helleday T (2009) Transcription-associated recombination in eukaryotes: link between transcription, replication and recombination. Mutagenesis 24: 203–210.
- 81. Alsford S, Horn D (2007) RNA polymerase I transcription stimulates homologous recombination in Trypanosoma brucei. Mol Biochem Parasitol 153: 77–79.
- 82. Alsford S, Horn D (2008) Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei. Mol Biochem Parasitol 161: 76–79.
- 83. Gonzalez-Barrera S, Garcia-Rubio M, Aguilera A (2002) Transcription and double-strand breaks induce similar mitotic recombination events in Saccharomyces cerevisiae. Genetics 162: 603–614.
- 84. Gottipati P, Cassel TN, Savolainen L, Helleday T (2008) Transcription-associated recombination is dependent on replication in Mammalian cells. Mol Cell Biol 28: 154–164.
- 85. Prado F, Aguilera A (2005) Impairment of replication fork progression mediates RNA polII transcription-associated recombination. EMBO J 24: 1267–1276.
- 86. Palmer GH, Brayton KA (2007) Gene conversion is a convergent strategy for pathogen antigenic variation. Trends Parasitol 23: 408–413.
- 87. Wirtz E, Leal S, Ochatt C, Cross GAM (1999) A tightly regulated inducible expression system for dominant negative approaches in Trypanosoma brucei. Mol Biochem Parasitol 99: 89–101.