Inflammation has long been implicated as a contributor to pathogenesis in many CNS illnesses, including Lyme neuroborreliosis. Borrelia burgdorferi is the spirochete that causes Lyme disease and it is known to potently induce the production of inflammatory mediators in a variety of cells. In experiments where B. burgdorferi was co-cultured in vitro with primary microglia, we observed robust expression and release of IL-6 and IL-8, CCL2 (MCP-1), CCL3 (MIP-1α), CCL4 (MIP-1β) and CCL5 (RANTES), but we detected no induction of microglial apoptosis. In contrast, SH-SY5Y (SY) neuroblastoma cells co-cultured with B. burgdorferi expressed negligible amounts of inflammatory mediators and also remained resistant to apoptosis. When SY cells were co-cultured with microglia and B. burgdorferi, significant neuronal apoptosis consistently occurred. Confocal microscopy imaging of these cell cultures stained for apoptosis and with cell type-specific markers confirmed that it was predominantly the SY cells that were dying. Microarray analysis demonstrated an intense microglia-mediated inflammatory response to B. burgdorferi including up-regulation in gene transcripts for TLR-2 and NFκβ. Surprisingly, a pathway that exhibited profound changes in regard to inflammatory signaling was triggering receptor expressed on myeloid cells-1 (TREM1). Significant transcript alterations in essential p53 pathway genes also occurred in SY cells cultured in the presence of microglia and B. burgdorferi, which indicated a shift from cell survival to preparation for apoptosis when compared to SY cells cultured in the presence of B. burgdorferi alone. Taken together, these findings indicate that B. burgdorferi is not directly toxic to SY cells; rather, these cells become distressed and die in the inflammatory surroundings generated by microglia through a bystander effect. If, as we hypothesized, neuronal apoptosis is the key pathogenic event in Lyme neuroborreliosis, then targeting microglial responses may be a significant therapeutic approach for the treatment of this form of Lyme disease.
Lyme disease, which is transmitted to humans through the bite of a tick, is currently the most frequently reported vector-borne illness in the northern hemisphere. Borrelia burgdorferi is the bacterium that causes Lyme disease and it is known to readily induce inflammation within a variety of infected tissues. Many of the neurological signs and symptoms that may affect patients with Lyme disease have been associated with B. burgdorferi-induced inflammation in the central nervous system (CNS). In this report we investigated which of the primary cell types residing in the CNS might be functioning to create the inflammatory environment that, in addition to helping clear the pathogen, could simultaneously be harming nearby neurons. We report findings that implicate microglia, a macrophage cell type in the CNS, as the key responders to infection with B. burgdorferi. We also present evidence indicating that this organism is not directly toxic to neurons; rather, a bystander effect is generated whereby the inflammatory surroundings created by microglia in response to B. burgdorferi may themselves be toxic to neuronal cells.
Citation: Myers TA, Kaushal D, Philipp MT (2009) Microglia Are Mediators of Borrelia burgdorferi–Induced Apoptosis in SH-SY5Y Neuronal Cells. PLoS Pathog 5(11): e1000659. https://doi.org/10.1371/journal.ppat.1000659
Editor: Jenifer Coburn, Medical College of Wisconsin, United States of America
Received: May 11, 2009; Accepted: October 19, 2009; Published: November 13, 2009
Copyright: © 2009 Myers et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants NS048952 and RR00164 from the National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Lyme borreliosis is the most prevalent vector-borne illness in the northern hemisphere ,. Transmission from the animal reservoir to the human host occurs via an Ixodes tick bite into the skin, where B. burgdorferi, the spirochete that causes Lyme disease, can then disseminate hematogenously to various organs, including the heart, joints and both the peripheral and central nervous systems –. A prominent, recurring and yet only partially understood feature of Lyme disease is the presence of inflammatory infiltrates within infected tissues –. Evidence of neurological involvement occurs to varying degrees in both the central and peripheral nervous systems of Lyme disease patients, and inflammation is often associated with the neurological manifestations that define neuroborreliosis , –,,. Upon gaining access to the central nervous system (CNS), B. burgdorferi may induce cerebrospinal fluid pleocytosis, meningoradiculitis and cranial neuritis as well as encephalopathies with neurocognitive abnormalities ,,,. This complex process of B. burgdorferi-induced pathology in the CNS has many aspects as yet to be clarified, with reactive inflammation potentially being one of the principal contributors to neuronal dysfunction.
Although B. burgdorferi lacks lipopolysaccharide (LPS), the organism does produce spirochetal lipoproteins that can induce inflammation –. By signaling through CD14, binding Toll-like receptors (TLR) 2 and 1 and subsequent activation of NFκβ, lipoproteins have been shown to generate inflammatory mediators in a variety of cell types , –. Recent studies with TLR2 and/or MyD88-deficient mice in which B. burgdorferi-induced inflammatory infiltrates were greater than that of wild type controls, indicated however, that there are alternative pathways regulating the inflammatory response to infection with B. burgdorferi ,,. As neuronal dysfunction is further analyzed in the light of these findings, it becomes necessary to consider which cells of the CNS milieu can function most aggressively/efficiently in creating an inflammatory environment that will contribute to clearance of the pathogen, but may in the process, harm nearby neurons.
Using in vitro and ex vivo experiments, investigators have shown that B. burgdorferi can induce potent production of inflammatory cytokines and chemokines in microglia, the resident macrophage cells of the CNS ,,,. The secretion of these mediators is likely a vital step in the development of inflammatory reactions, as chemokines, depending on their CC/CXC family sequence, may recruit distinct immune-effector cells, including monocytes, lymphocytes or neutrophils to sites of inflammation. Additionally, many cytokines and chemokines become involved in apoptosis, cell cycle regulation and angiogenesis , , , –. While a similar response is observed in astrocytes, the repertoire range and scale of concentration is typically much lower than that of activated microglia ,,. Importantly, although astrocytes are considered to be the primary CNS support cells, activated microglia can also secrete a host of soluble agents, such as glia-derived neurotrophic factor, that are potentially neuroprotective –. As the majority of molecules produced by activated microglia are however considered to be pro-inflammatory and neurotoxic ,,,, the microglial response to CNS infection with B. burgdorferi could tip the scales toward neuronal damage and death rather than survival in Lyme neuroborreliosis.
We have argued that the associated neurocognitive symptoms of Lyme neuroborreliosis may be the result of neuronal dysfunction resulting from inflammatory mediators released in response to infection with B. burgdorferi. Serving as an in vivo proof of concept to this hypothesis, our laboratory has recently shown the production of IL-6 by astrocytes, as well as the induction of oligodendrocyte and neuronal apoptosis in brain tissues taken from rhesus macaques that received intraparenchymal stereotaxic inoculations of live B. burgdorferi . With the goal of beginning to establish cause and effect relationships between glial cell responses to B. burgdorferi and neuronal apoptosis, we quantified the production/secretion of inflammatory cytokines and chemokines in purified rhesus brain cortex microglia and astrocytes, in human neuronal cells from the SH-SY5Y (SY) neuroblastoma cell line, and in combinations of the above cells co-cultured with live B. burgdorferi or recombinant purified lipidated outer-surface protein A (L-OspA). SY cells were cultured in three dimensions as opposed to monolayer culture, as we had shown that that mode of cultivation significantly narrows the phenotypic gap between neuronal cell lines and primary neurons . We also determined the extent of B. burgdorferi-induced apoptosis in each of the above cellular combinations. Using microarray analysis, we further examined the principal inflammation and apoptosis pathways affected by B. burgdorferi in these cells. Our findings suggest a bystander effect in which the neurotoxic surroundings generated by microglia may contribute to neuronal cell damage.
Materials and Methods
Brain tissues used in this study were collected from rhesus macaques (Macaca mulatta). These animals were not experimentally infected with B. burgdorferi and were culled from the breeding colony because of chronic diarrhea or injury. The procedure used for euthanasia was consistent with the recommendations of the American Veterinary Medical Association's Panel on Euthanasia and was approved by Tulane University's Institutional Animal Care and Use Committee.
Media and reagents
Dulbecco's modified Eagle's medium (D-MEM)-F-12 with L-glutamine and 15 mM HEPES buffer, D-MEM high glucose with L-glutamine, F-12 (Ham) with glutamax, penicillin (100 units/ml), streptomycin (100 units/ml), amphotericin B (0.25 µg/ml), non essential amino acids (NEAA) (100 units/ml), sodium pyruvate (100 units/ml), sodium bicarbonate (7.5% solution), trypsin (0.25%)/EDTA (0.38 g/ml), trypan blue™, normal goat serum (NGS), Alexa-562 (red)-conjugated secondary antibody, and the ToPro (blue) nuclear stain were each from Invitrogen. Primocin was from Invivogen. Fetal bovine serum was from Hyclone/Thermo Scientific and Cytodex-3™ micro-carrier beads were from Amersham Biosciences. Granulocyte-macrophage colony-stimulating factor (GM-CSF), L-leucine methyl ester (LME), Barbour-Stoenner-Kelly-H medium with rabbit serum, rifampicin, amphotericin, fish skin gelatin (FSG), propidium iodide (PI) (red) and anti-GFAP antibody were from Sigma-Aldrich. Lipidated (L-OspA) and unlipidated (U-OspA) outer surface protein A were a kind gift from GlaxoSmithKline. Paraformaldehyde (PFA, 2%) was from USB Corporation, and Triton X-100 from ICN Biochemicals. HuD primary antibody was from Santa Cruz Biotechnology. IBA-1 antibody was from WAKO.
Cell isolation and culture
Brain tissues used in this study were collected from rhesus macaques (Macaca mulatta). These animals were not experimentally infected with B. burgdorferi and were culled from the breeding colony because of chronic diarrhea or injury. Tissue was removed from the cortical region of the brain and processed as follows. After removal of the leptomeninges and leptomeningeal blood vessels, the cortex tissue was first dissociated mechanically by mincing the tissue with a sterile scalpel. Enzymatic dissociation followed by resuspending the minced tissue in D-MEM-F-12 medium with 0.25% trypsin-EDTA, 0.1% DNase and primocin (100 µg/ml), at 37°C for approximately 30 minutes, with occasional manual shaking. The solution was then centrifuged (425 x g, 10 minutes), the upper layer of cells removed, passed through a 20 µm Nitex filter and resuspended in D-MEM-F-12 with 10% FBS, 0.5 ng/ml GM-CSF and primocin antibiotic/antimycotic. After an incubation of approximately 10 days to 4 weeks at 37° in a 5% CO2, humid atmosphere incubator, microglia could be dislodged and recovered by vigorously tapping the culture flasks. The astrocyte cells were purified of any remaining microglia by removal of the GM-CSF component from the medium and subsequent treatment with 25 mM L-leucine methyl ester (LME) for 60 minutes at room temperature. Purity of both the astrocyte and microglial cell cultures was assessed to be approximately 98 percent and was confirmed by staining with GFAP and IBA-1. The culture was considered an aggregate of astrocytes and microglia before being purified for either specific cell type.
Human SY neuroblastoma cells [American Type Culture Collection (ATCC) CRL-2266] were seeded into T75 flasks with medium renewal every 3–7 days. Cell propagation was performed as per the ATCC product sheet. Three-dimensional (3-D) cultures were then prepared as previously described . For any SY cell co-culture with glia or B. burgdorferi, SY cell culture complete growth medium was used. This medium was composed of a 1∶1 mixture of D-MEM high glucose with L-glutamine and F-12 medium. Penicillin, streptomycin and amphotericin B were added to the medium only when spirochetes were not present. Trypsin/EDTA was used to dislodge the cells in both neuronal and glia cultures, and trypan blue stain was used to assess cell viability.
B. burgdorferi was cultured as previously described  with limited modifications. When combined in any co-culture with glia and/or SY cells, the described appropriate mammalian cell culture medium was used, but without the addition of antibiotics and antimycotic. While B. burgdorferi did not divide in any of the mammalian culture media used, spirochetal viability was determined to be above 85% in all assays. The LIVE/DEAD BacLight bacterial viability kit (Invitrogen) was used as per the manufacturer's instructions, to determine viability at both 24 hours and 5 days during co-culture with glia and/or SY cells. B. burgdorferi was additionally cultured in the mammalian cell medium alone to confirm proliferation rates and viability.
Three-micrometer pore diameter polyester transwell culture inserts (Becton Dickinson, Falcon) were incorporated into our co-culture models to physically separate SY cells from microglia. The SY cells were seeded directly into 24 well dishes (Costar) with the microglia then seeded onto culture inserts that had been placed into the same wells as the SY cells. The cell density in these experiments was 1×105 cells per ml of culture medium with an initial microglia to neuronal cell ratio of 4∶1. Live B. burgdorferi was added to the culture medium in both chambers for a 5-day stimulation, at an MOI of 10∶1, in relation to the entire cell population. In this way, it was possible to maintain a generally homogeneous exposure of each cell type to the culture medium while still allowing for bidirectional transfer of secreted molecules between the cell types.
Quantification of cytokines
Supernatants collected from primary glia and/or SY cells co-cultured with B. burgdorferi (MOI 10∶1) or in medium alone for either 24 hours or 5 days were used for quantification of secreted cytokines and chemokines. All of the mammalian cells were seeded at a density of 5×104 cells per 500 µl of culture medium with a glial cell to SY cell ratio of 4∶1. The 27-cytokine bioplex assays (Bio-Rad) were performed according to manufacturer's directions as were the individual antigen-capture ELISAs for IL-6, IL-8, TNF, CCL3, CCL4, and MCP-1 (CCL2). Sandwich ELISA capture and detection antibody pairs for human IL-6, IL-8 and TNF, along with recombinant human IL-6, IL-8, TNF and horseradish peroxidase (HRP) were from BD Biosciences. We obtained CCL3 and CCL4 ELISA DuoSet kits and the MCP-1 (CCL2) kit from R&D Systems.
Determination of nitric oxide production
Supernatants collected from primary glia and/or SY cells co-cultured with B. burgdorferi (MOI 10∶1) or in medium alone for either 24 hours or 5 days were used for quantitative determination of total secreted nitric oxide. The mammalian cells were seeded at a density of 5×104 cells per 500 µl of culture medium with an initial glial cell to SY cell ratio of 4∶1. The Total Nitric Oxide Assay Kit (catalogue #917–020) from Assay Designs was used, and all analyses were performed according to directions from the manufacturer.
Apoptosis assays, confocal microscopy
Microglia, astrocytes, SY cells or combinations thereof were cultured in the presence of B. burgdorferi (MOI 10∶1) or medium alone for either 24 hours or 5 days. After removal of the supernatant, the cells were harvested using trypsin, washed in phosphate buffered saline (PBS), and fixed for 5–10 minutes in 2% PFA. The fixed cells were permeabilized in PBS/FSG/Triton and blocked with 10% NGS. Apoptosis was evaluated using the Apoptag TUNEL assay kit (Chemicon/Millipore) as per manufacturer's instructions and the results were analyzed using a Leica TCS SP2 confocal microscope equipped with 3 lasers. Briefly, 6–18 0.2-µm optical slices per image were collected at 512×512 pixel resolution. In order to distinguish SY cells in the co-cultures containing glia, the cells were additionally stained with primary anti-HuD antibody for 1 hour, washed 3 times in PBS and then stained with Alexa 562-labeled secondary antibody for 45 minutes. The To-Pro nuclear stain was combined with the secondary antibody at a concentration equal to 0.05 µg/ml. The identities of microglia and astrocytes were additionally confirmed using the above protocol, substituting anti-IBA-1 and anti-GFAP respectively, for the anti-HuD. Cell morphology consistent with apoptosis including cell shrinkage, nuclear condensation and membrane blebbing was assessed along with the fluorescein staining for TUNEL. The number of apoptotic cells counted was divided by the total (500 minimum) number of cells counted. When the assays included co-culture of SY cells with glia, the number of cells that were double- stained for apoptosis and neuron specificity were divided by the total number of cells displaying the neuronal marker stain. Statistical significance was evaluated by One Way analysis of variance (ANOVA) followed by Bonferroni, Tukey and Levine's tests.
RNA was isolated from approximately 5×106 isolated SY cells or microglia using the RNeasy kit (Qiagen) plus DNA-free (Ambion) to eliminate DNA contamination. Five hundred nanogram of total RNA was amplified and used to synthesize Cy-labeled cDNA with the Low RNA Input Linear Amplification Kit (LRILAK, Agilent Technologies). Cy3 (control) and Cy5 (experimental) labeled cDNA were mixed in equimolar quantities and hybridized overnight at 55°C, to Agilent whole-genome microarrays (4×44 k format). While the samples derived from macaque microglia were hybridized to rhesus macaque arrays (Agilent # G2519F) with over 44,000 rhesus macaque probes, representing approximately 18,000 individual annotated genes, the samples derived from the neuronal cells were hybridized to human genome arrays (Agilent # G4112F) with over 41,000 oligonucleotides, representing approximately 22,000 unique human genes. The slides were scanned on a GenePix 4000B scanner, and data were extracted from the resulting 16-bit TIFF images using GenePix Pro 6.1 software. Data were analyzed using Spotfire DecisionSite for Microarray Analysis. Values were log2 transformed and normalized using a Locally Weighted ScatterPlot Smoothing (LOWESS) script within S+ ArrayAnalyzer.
RNA was isolated from approximately 5×106 isolated microglia or neuronal cells using the RNeasy kit (Qiagen) plus DNA-free (Ambion) to eliminate DNA contamination. The RNA was reverse-transcribed into DNA using a OneStep RT-PCR kit (Qiagen) and the QuantiFast™ SYBR® Green PCR kit (Qiagen) was then used for the quantitative real-time (QRT)-PCR. All assays were performed according to directions from the manufacturer and using Qiagen Quantitect® primer pairs in a 96-well block Applied Biosystems 7900 HT fast RT PCR System. PCR efficiencies, average fold change and statistical significance were evaluated using REST© software.
B. burgdorferi upregulates the expression of inflammatory cytokines/chemokines in primary rhesus glia but not in SY neurons
In experiments where B. burgdorferi or recombinant L-OspA was co-cultured with isolated rhesus cortex glia, we observed robust expression and release of IL-6 and IL-8 (Figure 1A). We also observed the production of TNF, although at a much lower level, and only by microglial cells and cells in the aggregate cultures, which themselves contained microglia (Figure 1A insert). SY cell production of IL-6 and IL-8 in response to B. burgdorferi and L-OspA were below the limit of detection for the assay used. Cytokine/chemokine expression levels in response to the same stimuli often varied significantly in glial cells obtained from different animals. The patterns of expression, however, were reproducible in experiments where the glial cells were isolated from tissue originating from a single individual animal. As such, the highest response was always that of microglia, followed by that of aggregate cultures and then astrocytes, regardless of the animal from whom the cells had been obtained. With the exception of TNF, whose expression peaked at 24 hours and then declined, levels of cytokine/chemokine expression also increased with the time of stimulation (Figure 1B).
(A) Sandwich ELISA shows prominent increases in IL-6 and IL-8 expression/secretion by primary cultures of rhesus cortex glia in the presence of lipidated outer-surface protein A (L-OspA): 0.25 µg/ml, or B. burgdorferi (Bb; MOI of 10∶1), for 24 hours, and (B) for 5 days. Unlipidated outer-surface protein A (U-OspA): 0.25 µg/ml, was used as the control for L-OspA. While a slight U-OspA-induced increase in IL-8 expression was observed in the microglia samples at 24 hours, this increase was not statistically different from control values. TNF expression was only observed in cultures containing microglia, where it peaked at 24 hours and was not observed at 5 days. Neuron responses were absent or negligible in comparison to that of the glial cells at both time points. In order to better visualize TNF expression, the Y axis in Figure 1A was expanded (insert). Values on each graph are from a single representative animal where depending on the stimulus and time of incubation, each experiment was repeated in 3 or more rhesus macaques. Error bars represent standard deviation of replicates on the plate where n = 3.
As the inherent difficulties in culturing primary neurons , often render their use in experiments impractical, the neuroblastoma cell line SH-SY5Y was employed to assess neuronal responses. We used a three-dimensional (3D) rather than traditional monolayer (2D) culture for the SY cells, as this culture method has been shown to promote a more normal, untransformed phenotype as compared to that of transformed cells grown in 2D , , –.
Neuronal apoptosis occurs in the inflammatory environment created by microglia in response to B. burgdorferi
In order to assess endpoint damage to the glia and SY cells responding to co-culture with B. burgdorferi or L-OspA, we employed the terminal deoxynuclease dUTP nick end labeled (TUNEL) assay as a tool for visualization of apoptosis. When microglia isolated either alone or in aggregate with astrocyte cells, were co-cultured with both B. burgdorferi and SY for at least 5 days, increases in cellular apoptosis consistently occurred. Apoptosis in glial cells was minimal as compared to the un-stimulated controls and there were no remarkable changes in apoptotic levels with regard to individual animals. SY cells cultured for 5 days with B. burgdorferi alone, or in combination with astrocytes and B. burgdorferi, showed only baseline levels of apoptosis (Table 1). No significant increase in astrocyte apoptosis was observed whether these cells were incubated with other cell types, L-OspA or B. burgdorferi (Table 1). Confocal microscopy images of mixed cultures stained for TUNEL and with cell-specific markers indicated that the majority of cells dying in response to B. burgdorferi were SY cells (Figure 2A). In consideration of these findings, we focused our next experiments on SY cells cultured in the presence of microglial cells and B. burgdorferi for 5 days. Significant increases in SY cell apoptosis occurred consistently in cell cultures from each of the 4 animals sampled when both microglia and B. burgdorferi were included in the culture conditions (Figure 2B).
(A) Cell specific markers and a separate nuclear stain were combined with the TUNEL assay. TUNEL: FITC (green), neuron specific marker: HuD (red), nucleus specific marker: To-Pro (blue). Confocal microscopy was used to determine that the majority of cells dying by apoptosis in response to B. burgdorferi were neurons. An accurate total cell number per field was established by counting all of the individual nuclei (blue). An on/off toggle of the laser channel for the nuclear stain (blue), would allow easy detection of overlap between the cell specific (red) and TUNEL (green) markers (lower panel). As the SY neuronal cells tend to grow in clusters, multiple panels were counted in order to acquire a proper percentage of each cell type. Total cell counts typically ranged between 500 and 1000. (B) In experiments focused on SY cells co-cultured with combinations of both microglia (µG) and B. burgdorferi (Bb), significant increases in SY, but not glial apoptosis consistently occurred. Cell culture in these experiments was performed as above, but the microglia and aggregate (Agg) cultures were obtained from 4 individual animals. Data represent the mean (n = 4) ± S.D. Δ = change. P values were obtained using One Way analysis of variance (ANOVA) followed by Bonferroni, Tukey and Levine's tests.
In order to determine whether stimulation of the culture medium with IL-6, IL-8, TNF or combinations thereof would be sufficient to induce apoptosis in the SY cells, we conducted experiments in which concentrations of each cytokine (recombinant-human) were added to the neuronal medium that were comparable to the highest expression values obtained during our in vitro assays with microglia and SY cells. After a 5 day stimulation, we did not find increases in neuronal apoptosis comparable to those observed in the co-culture of SY cells with microglia and B. burgdorferi, or significantly above the baseline levels of SY cells cultured in medium alone (data not shown).
Potent and sustained expression of the chemokines CCL2, CCL3, CCL4 and CCL5, in addition to that of IL-6 and IL-8, contributes to maintaining the inflammatory environment created by microglia in response to B. burgdorferi
In view of the evidence indicating that microglia were the more robust responders to the inflammatory stimuli of B. burgdorferi (Figure 1), and that molecules other than, or in addition to IL-6, IL-8 and TNF were required to elicit SY cell apoptosis, we further explored the diversity of mediators produced by microglia in response to B. burgdorferi. As several investigators had reported that the activated glia seen in many CNS pathologies were able to kill neurons by the release of nitric oxide (NO) into their surrounding environments –, and knowing that B. burgdorferi could elicit the release of NO from exposed macrophages , we explored this possibility in our models. Using an adaptation of the Greiss reaction, we were not able to detect any significant release of NO into the supernatant of microglia, astrocytes, SY cells or any combinations of the three cell types co-cultured with B. burgdorferi for either 24 hours or for 5 days (data not shown).
Using a 27 cytokine Bio-Plex assay to expand on our previous data, we found along with IL-6 and IL-8, significant B. burgdorferi-induced upregulation in expression of the pro-inflammatory chemokines CCL2, CCL3, CCL4 and CCL5 by microglial cells (Table 2). L-OspA (0.25 µg/ml) was observed to elicit a comparable upregulation of cytokines in both microglia and astrocytes, indicating that B. burgdorferi lipoproteins may elicit the lion's share of the spirochetal stimulus. However, when microglia were present in the cultures, alone or with astrocytes (aggregate cultures), it appeared that spirochetes provided, overall, the stronger stimulus. SY cell responses were absent or minimal in comparison to that of the microglia, except for the production of vascular endothelial growth factor (VEGF) (Table 2), which has been increasingly implicated as a contributing factor in neuronal protection and survival –.
B. burgdorferi induced significant cytokine/chemokine expression in astrocytes, but expression levels were generally orders of magnitude lower than those of microglia that had been derived from the same tissue (Table 2). These results, combined with our previous cytokine and apoptosis assays, prompted us to focus on interactions of microglia with SY cells in the presence of B. burgdorferi, and to broaden our exploration of pathways involved in microglial activation and neuronal apoptosis.
Multiple inflammatory signaling pathways are activated in microglia exposed to B. burgdorferi
We used microarray analysis to further investigate how exposure to B. burgdorferi might affect global gene expression in microglial cells. As changes in the expression and activity of multiple genes often work in concert to affect responses to many cellular pathogens, including B. burgdorferi , –, we used Ingenuity Pathways Analysis software to compare transcript levels in 18,000 annotated rhesus genes. When microglia were cultured in the presence of B. burgdorferi, as compared to medium alone, five of the ten most altered canonical pathways, as well as the chemokine signaling pathway (number 19 on the list of 232 affected pathways), showed significant upregulation in inflammatory signaling (Figure 3A).
(A) Five of the ten canonical pathways most affected during co-culture of microglia with B. burgdorferi involved inflammatory signaling (in bold) and occurred in cells isolated from each of two rhesus macaques sampled. The number in parenthesis indicates the pathway order of significance in the 2nd animal. Bar graph represents the ratio of gene expression in microglia co-cultured with B. burgdorferi as compared to control microglia cultured in medium alone. Line graph represents significance as –log (p-value) where p<0.05. (B) Microarray analysis of the triggering receptor expressed in myeloid cells (TREM1) pathway indicates, through transcript up-regulation of numerous pro-inflammatory genes including MCP-1 (CCL2), IL-6 and lL-8, the tendency for a sustained microglia-generated inflammatory response to B. burgdorferi. Values were obtained using Ingenuity Pathways Analysis (IPA) software, version 6. Minimum fold change was ≥2.0 with significance of p<0.05. All genes in this pathway were represented on the array chips. Red: increased gene expression. Green: decreased gene expression. The results displayed in sections (A) and (B) represent findings from animal number 1. Individual transcript changes for animals 1 and 2 can be accessed through the online supplemental data (Tables S1 and S2).
Many of the transcript changes that occurred within the triggering receptor expressed on myeloid cells-1 (TREM1), pattern recognition receptors (PRR), IL-10, IL-6 and chemokine signaling (Chem. Sig.) pathways were proinflammatory and they were repeated in several pathways that included both innate and adaptive immune responses (Table 3). Interestingly, the pathway that exhibited the most profound changes in regard to inflammatory signaling was TREM1 (Figure 3B, Table 4).
TREM1 cell surface receptors associate with the adaptor molecule DAP12 for signaling and function and have been classified as immune/inflammatory response amplifiers. Although the receptor has thus far not been found to be expressed on microglial cells , –, our array data indicate that it is expressed in rhesus microglia, or a subpopulation thereof, and is also upregulated during infection with B. burgdorferi. A low level of TREM1 expression in rhesus microglia was confirmed by RT-PCR (data not shown). RT-PCR was further used to confirm significant transcript up-regulation in the following three selected molecules: CCL2 (TREM1 and Chem. Sig. pathways), IL-6 (TREM1, PRR, IL-10 and IL-6 pathways) and CCL5 (PRR and Chem. Sig. pathways), (Table 5). Individual transcript changes for both animals sampled can be accessed online through the supplemental data portion of this report (Tables S1 and S2).
B. burgdorferi-activated microglia may influence p53 Signaling in SY cells
In addition to affecting the environment surrounding neurons exposed to B. burgdorferi, activated microglia may also affect gene expression of neurons themselves. Microarray analysis for the p53 Signaling pathway revealed a microglia-induced shift in the gene expression of SY cells co-cultured with B. burgdorferi from one of cell survival and proliferation to one more in line with cell cycle arrest and apoptosis (Figure 4A and 4B).
(A) Gene expression for p53 signaling in SY cells co-cultured with B. burgdorferi exhibits through up-regulation of transcript for protein kinase B (AKT), thrombospondin (TSP)-1 and Bcl2-like (Bcl-XL) combined with decreased transcript for damage-regulated autophagy modulator (DRAM) and p16, a mode of cell survival and proliferation. (B) The inclusion of microglial cells in the co-culture of SY cells with B. burgdorferi causes a shift in SY gene expression more in line with preparation for apoptosis than cell survival. There is increased transcript expression for pro-apoptotic phosphatase and tensin homolog (PTEN), Bcl-2-associated X protein (BAX) and phorbol-12-myristate-13-acetate-induced protein (NOXA)-1 genes combined with a reversal to decreased transcript for the anti-apoptotic molecule Bcl-xL. There is also increased expression of the cyclin-dependent kinase inhibitor, p21 and down-regulation in cyclin dependent kinase CDK2, both indicating a potential preparation for cell cycle arrest at G1/S. See also, Table 5. Values were obtained using Ingenuity Pathways Analysis (IPA) software version 6. Fold change was ≥1.5 with p≤0.05. The human array chips contained approximately 22,000 unique annotated genes and all genes in these pathways were represented on the chips. Red: increased gene expression. Green: decreased gene expression. Microglia were physically separated from the SY cells by 3.0-micrometer pore diameter transwell culture inserts. Individual transcript can be accessed through the online supplemental data (Tables S3 and Tables S4).
In cultures containing only SY cells and B. burgdorferi, there were prominent increases in transcript for the cell survival and anti-apoptotic molecules protein kinase B (AKT1) and BCL2-like (Bcl-XL) genes, combined with a decrease in transcript for pro-apoptotic damage-regulated autophagy modulator (DRAM) (Table 6-left, Figure 4A).
This scenario, however, changed dramatically in experiments when microglia were included in the co-culture of SY cells with B. burgdorferi (Figure 4B, Table 6, right). In order to study microglia-induced gene expression changes in the SY cells, transwell inserts were incorporated into the culture system providing physical separation between the SY cells and the microglial cells, yet allowing for bidirectional transfer of secreted molecules between the cell types. In this model, gene expression for phosphatase and tensin homolog (PTEN), which functions to modulate cell survival and proliferation primarily through its downstream effects on AKT1, was shown to be significantly increased (Table 6, right). SY cell anti-apoptotic Bcl-XL expression was observed to completely swing from up to down-regulation in the presence of microglia, while pro-apoptotic BCL2-associated X protein (Bax) and phorbol-12-myristate-13-acetate-induced protein 1 (NOXA-1) transcripts were increased. Additionally, the transcript for cyclin-dependent kinase 2 (CDK2), which typically drives the cell cycle, was down-regulated, and the G1/S cell cycle checkpoint inhibitors, cyclin-dependent kinase inhibitor 1A (p21) and glycogen synthase kinase 3 beta (GSK3β), were both increased (Figure 4, Table 6 right). The observed changes for individual gene transcript levels may be accessed online in the supplemental data portion of this report (Tables S3 and S4). RT-PCR was used to confirm transcript changes in selected molecules from co-cultures of the SY neurons with B. burgdorferi both in the presence and absence of microglia (Table 7).
The induction of neuronal apoptosis during co-culture with B. burgdorferi and microglia might require cell-cell contact between the SY cells and microglia
Several investigators have shown that when in the presence of certain stimuli, not only can macrophages/microglia induce apoptosis in neighboring cells through a bystander effect, but that there might be an additional requirement for cell-cell contact between the macrophages/microglia and the affected nearby cells –.
To address the question of whether the microglia-secreted mediators in our system were enough to stimulate SY cell apoptosis on their own, we again incorporated transwell inserts into our co-culture models to physically separate the microglia from the SY cells while at the same time, maintaining a generally homogeneous exposure of each cell type to the culture medium. Parallel assays for B. burgdorferi-induced apoptosis were set up where one model followed the same experimental design that was described in Figure 2, and the second model included the addition of transwell inserts into the co-culture system for the duration of the experiment. In each case, primary microglia or aggregate cell isolates from three separate non-human primates were combined in co-culture for 5 days with SY neurons and B. burgdorferi. Using the original co-culture format, we found an average 7.7-fold increase in neuronal apoptosis when SY cells were co-cultured with microglia and B. burgdorferi as compared to the SY cells cultured in medium alone. In contrast, no significant changes in apoptosis levels were observed when the SY cells were co-cultured with B. burgdorferi, with microglia, or with microglia and B. burgdorferi using the transwell inserts (Figure 5).
The TUNEL assay was used to evaluate apoptosis in SH-SY5Y (SY) neurons and microglia (μG) co-cultured with B. burgdorferi (Bb) for 5 days. An average 7.7 fold-increase in neuronal apoptosis was observed when SY cells were co-cultured with primary microglia and B. burgdorferi as compared to the SY cells cultured in medium alone. An increase in neuronal cell apoptosis was also observed during the co-culture of SY cells with primary microglia when B. burgdorferi was present. No statistical changes in apoptosis were noted between the SY cells cultured in medium alone and those co-cultured with either B. burgdorferi alone, with microglia or co-cultured with microglia and B. burgdorferi using 3.0-micrometer filter transwell inserts to physically separate the cells (i). Values represent the mean (n = 3) ± S.D. P values were obtained using One Way analysis of variance (ANOVA) followed by Bonferroni, Tukey and Levine's tests. Microglial cells in each experiment were all obtained from the same animal, but different animals were used as a source of cells for each of the three experiments.
Neuroinflammation is thought to be a contributing factor in a number of neurodegenerative disorders including Alzheimer's disease, Parkinson's disease and multiple sclerosis, as well as in Lyme neuroborreliosis ,,,. Inflammatory mediator levels are often elevated in these disorders suggesting that they are actively involved in the disease process ,,,. Because neurological symptoms do occur in many patients with Lyme disease, and cognitive impairment is often a part of this scenario, it was important to discover which mediators likely caused the effects of B. burgdorferi-induced damage in neurons. We hypothesized that the inflammatory environment generated during a possible in vivo exposure of glial cells to B. burgdorferi could harm neurons through a bystander effect. To address this hypothesis, we used in vitro experiments to demonstrate that with regard to microglia, astrocytes, and neurons, the fundamental triumvirate of cells in the CNS, it was the microglia that most aggressively responded to interaction with B. burgdorferi. In addition to inducing a pronounced and sustained production of cytokines and chemokines in microglial cells, B. burgdorferi also activated important inflammatory signaling pathways in these cells. Together, these responses potentially contributed to creating a reactive environment that was toxic to the SY cells. Interestingly, we also found that in addition to inducing SY cell apoptosis through a bystander effect, there might be a requirement for direct cell-cell contact between microglia and neurons for end-stage damage to occur.
In early experiments, we determined that when SY cells were co-cultured in the presence of microglia and B. burgdorferi for at least 5 days (MOI 10∶1), significant increases in SY, but not glial cell apoptosis occurred. Our experiments also ruled out any B. burgdorferi related upregulation in the production of nitric oxide. While previous reports had indicated that L-OspA would induce significant levels of apoptosis in primary rhesus astrocytes , the L-OspA concentration of 1 µg/ml used in those experiments was 4 times greater than the one used in the current experiments. Using a formula developed by Norgard, et al.  we approximated the total amount of outer surface lipoproteins correlating to the number of spirochetes used in our MOI. An L-OspA concentration of 0.25 µg/ml provided a quantitatively more realistic representation of lipoproteins present in B. burgdorferi at the MOI used in our studies. We further discovered that although B. burgdorferi did induce a potent expression and secretion of the inflammatory cytokines/chemokines IL-6, IL-8 and TNF in microglia, additional mediators were required to trigger neuronal cell apoptosis. These results prompted us to broaden our study of potential inflammatory mediators, and at the same time, to explore B. burgdorferi-activated pathways in microglia and SY cells that might be further contributing to the neurotoxicity demonstrated in the apoptosis assays.
By expanding our search for the expression of cytokines and chemokines that were potentially relevant to B. burgdorferi-induced neurotoxicity we found that in addition to IL-6, IL-8 and TNF, the four well known proinflammatory chemokines CCL2, CCL3, CCL4 and CCL5 were significantly upregulated in microglial cells co-cultured with B. burgdorferi. Upregulation of CC chemokines has been described both in murine models of neurocysticerosis and multiple sclerosis (experimental autoimmune encephalomyelitis, EAE) ,. CCL2 in particular has been shown to play a role in the pathogenesis of EAE . Perhaps most interesting were the microarray results showing that each of these molecules, with the exception of CCL3, was represented in more than one of the microglial signaling pathways that were most affected by B. burgdorferi. Considering the high number of inflammatory genes involved in these pathways and their potential for cross-talk and downstream regulation, we believe that the TREM1 pathway, together with those involving PRR, IL-10, IL-6 and Chem. Sig., contribute to B. burgdorferi-induced inflammation in the CNS.
Even though some of the remaining affected pathways may have provided checks and balances to our findings, many of the transcript changes that occurred in the pathways that we focused on were proinflammatory and included both innate and adaptive immune response molecules. As such, there seems to be a consistent pattern of inflammatory signaling in microglial cells that is directly associated with the presence of B. burgdorferi, and that might adversely affect nearby neuronal cells.
When glial cells were co-cultured with B. burgdorferi in the presence of SY cells the concentration of inflammatory mediators was often reduced as compared to that elicited in the absence of SY cells (Table 2). Since we saw no evidence of glial cell apoptosis, this decrease is most likely due to the reduced number of glial cells, the main producers of pro-inflammatory mediators that were included in these cultures. There are reports that apoptotic neurons co-cultured with microglia may down-regulate microglial synthesis of pro-inflammatory molecules . Such a mechanism also may have contributed to the observed reduction, at least in the case of microglia/SY cell co-cultures.
Using microarray analysis, we also showed that B. burgdorferi-activated microglia could invoke changes in their cellular environment that affected gene expression in proximal SY cells. When SY cells were co-cultured with B. burgdorferi alone, SY gene expression for molecules in the p53 signaling pathway indicated a mode of cell survival and proliferation. This conclusion was founded on the observed upregulation in transcripts for AKT1 and Bcl-XL, paired with decreased gene expression for DRAM. AKT1, which is activated through the phosphatidylinositol 3-kinase (PI3K) pathway, is known to stimulate cell cycle progression (via p21 phosphorylation and release from CDK2) and to play an important role in promoting cell survival through the suppression of apoptosis -. Bcl-XL is likewise a strong promoter of cell survival, but as one of the major anti-apoptotic members of the conserved Bcl-2 family of proteins, it functions to inhibit programmed cell death through the control of mitochondrial membrane permeabilization –. DRAM on the other hand, is a lysosomal protein critical for the ability of p53 to induce either autophagy or programmed cell death –.
In parallel experiments where microglia were included in the co-culture of SY cells with B. burgdorferi, but physically separated from the SY cells by transwell inserts, we found a striking change of circumstance. One primary difference observed with the inclusion of microglial cells was increased SY cell gene expression of the tumor suppressor PTEN. This molecule acts as a dual protein and lipid phosphatase that can modulate cell survival, proliferation and apoptosis through its downstream effects on phosphatidylinositol 3-kinase (PI3K) and the AKT1 gene ,,. Complementing the PTEN regulation of AKT1 was a decrease in transcript for CDK2 combined with transcript increases for the CDK inhibitors p21 and GSK3β. Within the cell cycle machinery, cyclin-dependent kinases (CDKs) associate with their respective cyclins to drive a cell through the G1/S checkpoint. CDK2 associates with cyclin E and during events of cell stress or DNA damage, p21 can bind the CDK2/cyclinE complex and induce cell cycle arrest at G1/S. Furthermore, association of the CDK4 with cyclin D1 can also be disrupted by the action of GSK3β, which phosphorylates cyclin D1, triggering its nuclear export and degradation –. Both of these events, if realized, would indicate a drift toward cell cycle arrest, which was not apparent in the transcript changes for the culture of SY cells with B. burgdorferi, but lacking microglia.
Other significant changes in gene expression were found in members of the apoptotic Bcl-2 family. Transcript of the anti-apoptotic Bcl-XL molecule, whose function was described previously, changed from being up-regulated when microglia were not present to being markedly down-regulated in the neuron/microglia/B. burgdorferi co-cultures. At the same time, expression of the pro-apoptotic Bax and NOXA-1 transcripts was found to be increased. Under normal conditions, Bax is constitutively associated with ly-XL. During cellular stress, Bax can be activated by a BH3-only protein such as Bid, Bim or NOXA-1. It may also be displaced by Bad or Bik molecules which possess Bad-like BH3 domains, but act directly on Bcl-2 or Bcl-XL rather than Bax itself, freeing it from Bcl-XL to translocate from the cytosol and directly bind to the mitochondrial membrane, initiating an apoptotic cascade , , –. AKT1 kinase is responsible for the phosphorylation of Bad, keeping it tied to its support molecule 14-3-3. In an inactivated state, such as that induced by the up-regulation of PTEN, Bad may become unphosphorylated, disassociate from 14-3-3 and move to displace Bax ,. While the results of in vitro experiments may not always mirror in vivo responses, this compilation of neuronal transcript changes in the p53 signaling pathway appears to be predicated on the presence of microglia, and provides a compelling example of the neurotoxic effects that activated microglia may have on neurons in their surroundings.
Finally, although we have presented evidence of the ability of microglia to harm SY cells through a bystander effect, our findings indicate that microglia-SY cell proximity, while necessary, may not be sufficient for end-stage SY cell damage/apoptosis to occur. When transwell filter inserts were incorporated into our apoptosis experiments, no significant increases in SY cell death were observed regardless of whether microglia were included in the co-cultures of SY cells with B. burgdorferi or not. This differed significantly from a near 8-fold increase in SY cell apoptosis that occurred when microglia (from the same animal) and SY cells were in direct physical contact during their co-culture with B. burgdorferi, indicating that neuronal-microglia contact was required for apoptosis to occur. Several investigators have reported a similar cell-cell contact requirement for apoptosis induced by activated macrophages and other non-neuronal cells –,. Whether their contact with mediators secreted by B. burgdorferi-activated microglia is itself enough to critically damage SY cells, or it simply acts to prime the cells for eventual apoptosis, remains in question. Due to viability constraints of the cell culture medium, we were not able to extend our apoptosis assays beyond 5 days. We therefore cannot exclude the possibility that longer exposure to microglial mediators might have induced apoptosis in SY cells. If this were the case, the cell-cell contact hypothesis would shift from one of “requirement” to one of “influence”. Further, even though steps were taken to more normalize the SY cell-line phenotype, these cells may respond differently than would non-transformed neurons. While experiments with primary neurons would be optimal, they are often not practical. Similar experiments with other cell lines and even primary neurons may be critical to understanding how B. burgdorferi affects neurons of the CNS during neuroborreliosis.
Microglia + Borrelia burgdorferi vs. Microglia alone, animal 1
(0.45 MB PDF)
Microglia + Borrelia burgdorferi vs. Microglia alone, animal 2
(0.23 MB PDF)
SY3D + B. burgdorferi vs. SY3D alone
(0.28 MB PDF)
We thank Robin Rodriguez for technical assistance with figures, Lisa Morici for technical assistance with the Bioplex assay and Monica Embers, Mary B. Jacobs and Andrea Bernardino for editorial comments.
Conceived and designed the experiments: TAM MTP. Performed the experiments: TAM. Analyzed the data: TAM DK MTP. Contributed reagents/materials/analysis tools: DK. Wrote the paper: TAM MTP.
- 1. Burns MJ, Furie MB (1998) Borrelia burgdorferi and interleukin-1 promote the transendothelial migration of monocytes in vitro by different mechanisms. Infect Immun 66: 4875–4883.MJ BurnsMB Furie1998Borrelia burgdorferi and interleukin-1 promote the transendothelial migration of monocytes in vitro by different mechanisms.Infect Immun6648754883
- 2. Cassiani-Ingoni R, Cabral ES, Lunemann JD, Garza Z, Magnus T, et al. (2006) Borrelia burgdorferi Induces TLR1 and TLR2 in human microglia and peripheral blood monocytes but differentially regulates HLA-class II expression. J Neuropathol Exp Neurol 65: 540–548.R. Cassiani-IngoniES CabralJD LunemannZ. GarzaT. Magnus2006Borrelia burgdorferi Induces TLR1 and TLR2 in human microglia and peripheral blood monocytes but differentially regulates HLA-class II expression.J Neuropathol Exp Neurol65540548
- 3. Grygorczuk S, Zajkowska J, Swierzbinska R, Pancewicz S, Kondrusik M, et al. (2005) Concentration of interferon-inducible T cell chemoattractant and monocyte chemotactic protein-1 in serum and cerebrospinal fluid of patients with Lyme borreliosis. Rocz Akad Med Bialymst 50: 173–178.S. GrygorczukJ. ZajkowskaR. SwierzbinskaS. PancewiczM. Kondrusik2005Concentration of interferon-inducible T cell chemoattractant and monocyte chemotactic protein-1 in serum and cerebrospinal fluid of patients with Lyme borreliosis.Rocz Akad Med Bialymst50173178
- 4. Kisand KE, Prukk T, Kisand KV, Luus SM, Kalbe I, et al. (2007) Propensity to excessive proinflammatory response in chronic Lyme borreliosis. Apmis 115: 134–141.KE KisandT. PrukkKV KisandSM LuusI. Kalbe2007Propensity to excessive proinflammatory response in chronic Lyme borreliosis.Apmis115134141
- 5. Livengood JA, Gilmore RD, Jr. (2006) Invasion of human neuronal and glial cells by an infectious strain of Borrelia burgdorferi. Microbes Infect 8: 2832–2840.JA LivengoodRD, Gilmore Jr.2006Invasion of human neuronal and glial cells by an infectious strain of Borrelia burgdorferi.Microbes Infect828322840
- 6. Ramesh G, Borda JT, Dufour J, Kaushal D, Ramamoorthy R, et al. (2008) Interaction of the Lyme disease spirochete Borrelia burgdorferi with brain parenchyma elicits inflammatory mediators from glial cells as well as glial and neuronal apoptosis. Am J Pathol 173: 1415–1427.G. RameshJT BordaJ. DufourD. KaushalR. Ramamoorthy2008Interaction of the Lyme disease spirochete Borrelia burgdorferi with brain parenchyma elicits inflammatory mediators from glial cells as well as glial and neuronal apoptosis.Am J Pathol17314151427
- 7. Rupprecht TA, Koedel U, Fingerle V, Pfister HW (2008) The pathogenesis of Lyme neuroborreliosis: from infection to inflammation. Mol Med 14: 205–212.TA RupprechtU. KoedelV. FingerleHW Pfister2008The pathogenesis of Lyme neuroborreliosis: from infection to inflammation.Mol Med14205212
- 8. Steere AC (2001) Lyme disease. N Engl J Med 345: 115–125.AC Steere2001Lyme disease.N Engl J Med345115125
- 9. Cadavid D, O'Neill T, Schaefer H, Pachner AR (2000) Localization of Borrelia burgdorferi in the nervous system and other organs in a nonhuman primate model of Lyme disease. Lab Invest 80: 1043–1054.D. CadavidT. O'NeillH. SchaeferAR Pachner2000Localization of Borrelia burgdorferi in the nervous system and other organs in a nonhuman primate model of Lyme disease.Lab Invest8010431054
- 10. Pachner AR, Steiner I (2007) Lyme neuroborreliosis: infection, immunity, and inflammation. Lancet Neurol 6: 544–552.AR PachnerI. Steiner2007Lyme neuroborreliosis: infection, immunity, and inflammation.Lancet Neurol6544552
- 11. Behera AK, Hildebrand E, Uematsu S, Akira S, Coburn J, et al. (2006) Identification of a TLR-independent pathway for Borrelia burgdorferi-induced expression of matrix metalloproteinases and inflammatory mediators through binding to integrin alpha 3 beta 1. J Immunol 177: 657–664.AK BeheraE. HildebrandS. UematsuS. AkiraJ. Coburn2006Identification of a TLR-independent pathway for Borrelia burgdorferi-induced expression of matrix metalloproteinases and inflammatory mediators through binding to integrin alpha 3 beta 1.J Immunol177657664
- 12. Bernardino AL, Myers TA, Alvarez X, Hasegawa A, Philipp MT (2008) Toll-like receptors: insights into their possible role in the pathogenesis of Lyme neuroborreliosis. Infect Immun 76: 4385–4395.AL BernardinoTA MyersX. AlvarezA. HasegawaMT Philipp2008Toll-like receptors: insights into their possible role in the pathogenesis of Lyme neuroborreliosis.Infect Immun7643854395
- 13. Fraser CM, Casjens S, Huang WM, Sutton GG, Clayton R, et al. (1997) Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390: 580–586.CM FraserS. CasjensWM HuangGG SuttonR. Clayton1997Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.Nature390580586
- 14. Giambartolomei GH, Dennis VA, Lasater BL, Murthy PK, Philipp MT (2002) Autocrine and exocrine regulation of interleukin-10 production in THP-1 cells stimulated with Borrelia burgdorferi lipoproteins. Infect Immun 70: 1881–1888.GH GiambartolomeiVA DennisBL LasaterPK MurthyMT Philipp2002Autocrine and exocrine regulation of interleukin-10 production in THP-1 cells stimulated with Borrelia burgdorferi lipoproteins.Infect Immun7018811888
- 15. Ma Y, Weis JJ (1993) Borrelia burgdorferi outer surface lipoproteins OspA and OspB possess B-cell mitogenic and cytokine-stimulatory properties. Infect Immun 61: 3843–3853.Y. MaJJ Weis1993Borrelia burgdorferi outer surface lipoproteins OspA and OspB possess B-cell mitogenic and cytokine-stimulatory properties.Infect Immun6138433853
- 16. Norgard MV, Arndt LL, Akins DR, Curetty LL, Harrich DA, et al. (1996) Activation of human monocytic cells by Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides proceeds via a pathway distinct from that of lipopolysaccharide but involves the transcriptional activator NF-kappa B. Infect Immun 64: 3845–3852.MV NorgardLL ArndtDR AkinsLL CurettyDA Harrich1996Activation of human monocytic cells by Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides proceeds via a pathway distinct from that of lipopolysaccharide but involves the transcriptional activator NF-kappa B.Infect Immun6438453852
- 17. Takayama K, Rothenberg RJ, Barbour AG (1987) Absence of lipopolysaccharide in the Lyme disease spirochete, Borrelia burgdorferi. Infect Immun 55: 2311–2313.K. TakayamaRJ RothenbergAG Barbour1987Absence of lipopolysaccharide in the Lyme disease spirochete, Borrelia burgdorferi.Infect Immun5523112313
- 18. Akira S, Uematsu S, Takeuchi O (2006) Pathogen recognition and innate immunity. Cell 124: 783–801.S. AkiraS. UematsuO. Takeuchi2006Pathogen recognition and innate immunity.Cell124783801
- 19. Giambartolomei GH, Dennis VA, Lasater BL, Philipp MT (1999) Induction of pro- and anti-inflammatory cytokines by Borrelia burgdorferi lipoproteins in monocytes is mediated by CD14. Infect Immun 67: 140–147.GH GiambartolomeiVA DennisBL LasaterMT Philipp1999Induction of pro- and anti-inflammatory cytokines by Borrelia burgdorferi lipoproteins in monocytes is mediated by CD14.Infect Immun67140147
- 20. Salazar JC, Pope CD, Moore MW, Pope J, Kiely TG, et al. (2005) Lipoprotein-dependent and -independent immune responses to spirochetal infection. Clin Diagn Lab Immunol 12: 949–958.JC SalazarCD PopeMW MooreJ. PopeTG Kiely2005Lipoprotein-dependent and -independent immune responses to spirochetal infection.Clin Diagn Lab Immunol12949958
- 21. Town T, Nikolic V, Tan J (2005) The microglial “activation” continuum: from innate to adaptive responses. J Neuroinflammation 2: 24.T. TownV. NikolicJ. Tan2005The microglial “activation” continuum: from innate to adaptive responses.J Neuroinflammation224
- 22. Behera AK, Hildebrand E, Bronson RT, Perides G, Uematsu S, et al. (2006) MyD88 deficiency results in tissue-specific changes in cytokine induction and inflammation in interleukin-18-independent mice infected with Borrelia burgdorferi. Infect Immun 74: 1462–1470.AK BeheraE. HildebrandRT BronsonG. PeridesS. Uematsu2006MyD88 deficiency results in tissue-specific changes in cytokine induction and inflammation in interleukin-18-independent mice infected with Borrelia burgdorferi.Infect Immun7414621470
- 23. Guerau-de-Arellano M, Huber BT (2005) Chemokines and Toll-like receptors in Lyme disease pathogenesis. Trends Mol Med 11: 114–120.M. Guerau-de-ArellanoBT Huber2005Chemokines and Toll-like receptors in Lyme disease pathogenesis.Trends Mol Med11114120
- 24. Rasley A, Anguita J, Marriott I (2002) Borrelia burgdorferi induces inflammatory mediator production by murine microglia. J Neuroimmunol 130: 22–31.A. RasleyJ. AnguitaI. Marriott2002Borrelia burgdorferi induces inflammatory mediator production by murine microglia.J Neuroimmunol1302231
- 25. Cruz AR, Moore MW, La Vake CJ, Eggers CH, Salazar JC, et al. (2008) Phagocytosis of Borrelia burgdorferi, the Lyme disease spirochete, potentiates innate immune activation and induces apoptosis in human monocytes. Infect Immun 76: 56–70.AR CruzMW MooreCJ La VakeCH EggersJC Salazar2008Phagocytosis of Borrelia burgdorferi, the Lyme disease spirochete, potentiates innate immune activation and induces apoptosis in human monocytes.Infect Immun765670
- 26. Sprenger H, Krause A, Kaufmann A, Priem S, Fabian D, et al. (1997) Borrelia burgdorferi induces chemokines in human monocytes. Infect Immun 65: 4384–4388.H. SprengerA. KrauseA. KaufmannS. PriemD. Fabian1997Borrelia burgdorferi induces chemokines in human monocytes.Infect Immun6543844388
- 27. Sprenger H, Rosler A, Tonn P, Braune HJ, Huffmann G, et al. (1996) Chemokines in the cerebrospinal fluid of patients with meningitis. Clin Immunol Immunopathol 80: 155–161.H. SprengerA. RoslerP. TonnHJ BrauneG. Huffmann1996Chemokines in the cerebrospinal fluid of patients with meningitis.Clin Immunol Immunopathol80155161
- 28. Banisor I, Leist TP, Kalman B (2005) Involvement of beta-chemokines in the development of inflammatory demyelination. J Neuroinflammation 2: 7.I. BanisorTP LeistB. Kalman2005Involvement of beta-chemokines in the development of inflammatory demyelination.J Neuroinflammation27
- 29. Saura J (2007) Microglial cells in astroglial cultures: a cautionary note. J Neuroinflammation 4: 26.J. Saura2007Microglial cells in astroglial cultures: a cautionary note.J Neuroinflammation426
- 30. Liu B, Hong JS (2003) Role of microglia in inflammation-mediated neurodegenerative diseases: mechanisms and strategies for therapeutic intervention. J Pharmacol Exp Ther 304: 1–7.B. LiuJS Hong2003Role of microglia in inflammation-mediated neurodegenerative diseases: mechanisms and strategies for therapeutic intervention.J Pharmacol Exp Ther30417
- 31. Minghetti L (2005) Role of inflammation in neurodegenerative diseases. Curr Opin Neurol 18: 315–321.L. Minghetti2005Role of inflammation in neurodegenerative diseases.Curr Opin Neurol18315321
- 32. Minghetti L, Ajmone-Cat MA, De Berardinis MA, De Simone R (2005) Microglial activation in chronic neurodegenerative diseases: roles of apoptotic neurons and chronic stimulation. Brain Res Brain Res Rev 48: 251–256.L. MinghettiMA Ajmone-CatMA De BerardinisR. De Simone2005Microglial activation in chronic neurodegenerative diseases: roles of apoptotic neurons and chronic stimulation.Brain Res Brain Res Rev48251256
- 33. Aloisi F (1999) The role of microglia and astrocytes in CNS immune surveillance and immunopathology. Adv Exp Med Biol 468: 123–133.F. Aloisi1999The role of microglia and astrocytes in CNS immune surveillance and immunopathology.Adv Exp Med Biol468123133
- 34. Myers TA, Nickerson CA, Kaushal D, Ott CM, Honer zu Bentrup K, et al. (2008) Closing the phenotypic gap between transformed neuronal cell lines in culture and untransformed neurons. J Neurosci Methods 174: 31–41.TA MyersCA NickersonD. KaushalCM OttK. Honer zu Bentrup2008Closing the phenotypic gap between transformed neuronal cell lines in culture and untransformed neurons.J Neurosci Methods1743141
- 35. Encinas M, Iglesias M, Liu Y, Wang H, Muhaisen A, et al. (2000) Sequential treatment of SH-SY5Y cells with retinoic acid and brain-derived neurotrophic factor gives rise to fully differentiated, neurotrophic factor-dependent, human neuron-like cells. J Neurochem 75: 991–1003.M. EncinasM. IglesiasY. LiuH. WangA. Muhaisen2000Sequential treatment of SH-SY5Y cells with retinoic acid and brain-derived neurotrophic factor gives rise to fully differentiated, neurotrophic factor-dependent, human neuron-like cells.J Neurochem759911003
- 36. Smith CUM (1996) Elements of Molecular Neurobiology. Chichester: John Wiley and Sons, Ltd. 522 p.CUM Smith1996Elements of Molecular Neurobiology.ChichesterJohn Wiley and Sons, Ltd522
- 37. Abbott A (2003) Cell culture: biology's new dimension. Nature 424: 870–872.A. Abbott2003Cell culture: biology's new dimension.Nature424870872
- 38. Hammond TG, Hammond JM (2001) Optimized suspension culture: the rotating-wall vessel. Am J Physiol Renal Physiol 281: F12–25.TG HammondJM Hammond2001Optimized suspension culture: the rotating-wall vessel.Am J Physiol Renal Physiol281F1225
- 39. Honer zu Bentrup K, Ramamurthy R, Ott CM, Emami K, Nelman-Gonzalez M, et al. (2006) Three-dimensional organotypic models of human colonic epithelium to study the early stages of enteric salmonellosis. Microbes Infect 8: 1813–1825.K. Honer zu BentrupR. RamamurthyCM OttK. EmamiM. Nelman-Gonzalez2006Three-dimensional organotypic models of human colonic epithelium to study the early stages of enteric salmonellosis.Microbes Infect818131825
- 40. LaMarca HL, Ott CM, Honer Zu Bentrup K, Leblanc CL, Pierson DL, et al. (2005) Three-dimensional growth of extravillous cytotrophoblasts promotes differentiation and invasion. Placenta 26: 709–720.HL LaMarcaCM OttK. Honer Zu BentrupCL LeblancDL Pierson2005Three-dimensional growth of extravillous cytotrophoblasts promotes differentiation and invasion.Placenta26709720
- 41. Lombet A, Zujovic V, Kandouz M, Billardon C, Carvajal-Gonzalez S, et al. (2001) Resistance to induced apoptosis in the human neuroblastoma cell line SK-N-SH in relation to neuronal differentiation. Role of Bcl-2 protein family. Eur J Biochem 268: 1352–1362.A. LombetV. ZujovicM. KandouzC. BillardonS. Carvajal-Gonzalez2001Resistance to induced apoptosis in the human neuroblastoma cell line SK-N-SH in relation to neuronal differentiation. Role of Bcl-2 protein family.Eur J Biochem26813521362
- 42. Nickerson CA, Goodwin TJ, Terlonge J, Ott CM, Buchanan KL, et al. (2001) Three-dimensional tissue assemblies: novel models for the study of Salmonella enterica serovar Typhimurium pathogenesis. Infect Immun 69: 7106–7120.CA NickersonTJ GoodwinJ. TerlongeCM OttKL Buchanan2001Three-dimensional tissue assemblies: novel models for the study of Salmonella enterica serovar Typhimurium pathogenesis.Infect Immun6971067120
- 43. Nickerson CA, Richter EG, Ott CM (2007) Studying host-pathogen interactions in 3-D: organotypic models for infectious disease and drug development. J Neuroimmune Pharmacol 2: 26–31.CA NickersonEG RichterCM Ott2007Studying host-pathogen interactions in 3-D: organotypic models for infectious disease and drug development.J Neuroimmune Pharmacol22631
- 44. Schmeichel KL, Bissell MJ (2003) Modeling tissue-specific signaling and organ function in three dimensions. J Cell Sci 116: 2377–2388.KL SchmeichelMJ Bissell2003Modeling tissue-specific signaling and organ function in three dimensions.J Cell Sci11623772388
- 45. Zhang S (2004) Beyond the Petri dish. Nat Biotechnol 22: 151–152.S. Zhang2004Beyond the Petri dish.Nat Biotechnol22151152
- 46. Ciriolo MR, De Martino A, Lafavia E, Rossi L, Carri MT, et al. (2000) Cu, Zn-superoxide dismutase-dependent apoptosis induced by nitric oxide in neuronal cells. J Biol Chem 275: 5065–5072.MR CirioloA. De MartinoE. LafaviaL. RossiMT Carri2000Cu, Zn-superoxide dismutase-dependent apoptosis induced by nitric oxide in neuronal cells.J Biol Chem27550655072
- 47. Lehnardt S, Henneke P, Lien E, Kasper DL, Volpe JJ, et al. (2006) A mechanism for neurodegeneration induced by group B streptococci through activation of the TLR2/MyD88 pathway in microglia. J Immunol 177: 583–592.S. LehnardtP. HennekeE. LienDL KasperJJ Volpe2006A mechanism for neurodegeneration induced by group B streptococci through activation of the TLR2/MyD88 pathway in microglia.J Immunol177583592
- 48. Mander P, Brown GC (2005) Activation of microglial NADPH oxidase is synergistic with glial iNOS expression in inducing neuronal death: a dual-key mechanism of inflammatory neurodegeneration. J Neuroinflammation 2: 20.P. ManderGC Brown2005Activation of microglial NADPH oxidase is synergistic with glial iNOS expression in inducing neuronal death: a dual-key mechanism of inflammatory neurodegeneration.J Neuroinflammation220
- 49. Ma Y, Seiler KP, Tai KF, Yang L, Woods M, et al. (1994) Outer surface lipoproteins of Borrelia burgdorferi stimulate nitric oxide production by the cytokine-inducible pathway. Infect Immun 62: 3663–3671.Y. MaKP SeilerKF TaiL. YangM. Woods1994Outer surface lipoproteins of Borrelia burgdorferi stimulate nitric oxide production by the cytokine-inducible pathway.Infect Immun6236633671
- 50. Ogunshola OO, Antic A, Donoghue MJ, Fan SY, Kim H, et al. (2002) Paracrine and autocrine functions of neuronal vascular endothelial growth factor (VEGF) in the central nervous system. J Biol Chem 277: 11410–11415.OO OgunsholaA. AnticMJ DonoghueSY FanH. Kim2002Paracrine and autocrine functions of neuronal vascular endothelial growth factor (VEGF) in the central nervous system.J Biol Chem2771141011415
- 51. Matsuzaki H, Tamatani M, Yamaguchi A, Namikawa K, Kiyama H, et al. (2001) Vascular endothelial growth factor rescues hippocampal neurons from glutamate-induced toxicity: signal transduction cascades. Faseb J 15: 1218–1220.H. MatsuzakiM. TamataniA. YamaguchiK. NamikawaH. Kiyama2001Vascular endothelial growth factor rescues hippocampal neurons from glutamate-induced toxicity: signal transduction cascades.Faseb J1512181220
- 52. Nicoletti JN, Shah SK, McCloskey DP, Goodman JH, Elkady A, et al. (2008) Vascular endothelial growth factor is up-regulated after status epilepticus and protects against seizure-induced neuronal loss in hippocampus. Neuroscience 151: 232–241.JN NicolettiSK ShahDP McCloskeyJH GoodmanA. Elkady2008Vascular endothelial growth factor is up-regulated after status epilepticus and protects against seizure-induced neuronal loss in hippocampus.Neuroscience151232241
- 53. Rosenstein JM, Mani N, Khaibullina A, Krum JM (2003) Neurotrophic effects of vascular endothelial growth factor on organotypic cortical explants and primary cortical neurons. J Neurosci 23: 11036–11044.JM RosensteinN. ManiA. KhaibullinaJM Krum2003Neurotrophic effects of vascular endothelial growth factor on organotypic cortical explants and primary cortical neurons.J Neurosci231103611044
- 54. Bermpohl D, Halle A, Freyer D, Dagand E, Braun JS, et al. (2005) Bacterial programmed cell death of cerebral endothelial cells involves dual death pathways. J Clin Invest 115: 1607–1615.D. BermpohlA. HalleD. FreyerE. DagandJS Braun2005Bacterial programmed cell death of cerebral endothelial cells involves dual death pathways.J Clin Invest11516071615
- 55. Colonna M (2003) TREMs in the immune system and beyond. Nat Rev Immunol 3: 445–453.M. Colonna2003TREMs in the immune system and beyond.Nat Rev Immunol3445453
- 56. Embers ME, Ramamoorthy R, Philipp MT (2004) Survival strategies of Borrelia burgdorferi, the etiologic agent of Lyme disease. Microbes Infect 6: 312–318.ME EmbersR. RamamoorthyMT Philipp2004Survival strategies of Borrelia burgdorferi, the etiologic agent of Lyme disease.Microbes Infect6312318
- 57. Bleharski JR, Kiessler V, Buonsanti C, Sieling PA, Stenger S, et al. (2003) A role for triggering receptor expressed on myeloid cells-1 in host defense during the early-induced and adaptive phases of the immune response. J Immunol 170: 3812–3818.JR BleharskiV. KiesslerC. BuonsantiPA SielingS. Stenger2003A role for triggering receptor expressed on myeloid cells-1 in host defense during the early-induced and adaptive phases of the immune response.J Immunol17038123818
- 58. Colonna M, Facchetti F (2003) TREM-1 (triggering receptor expressed on myeloid cells): a new player in acute inflammatory responses. J Infect Dis 187: Suppl 2S397–401.M. ColonnaF. Facchetti2003TREM-1 (triggering receptor expressed on myeloid cells): a new player in acute inflammatory responses.J Infect Dis187Suppl 2S397401
- 59. Dower K, Ellis DK, Saraf K, Jelinsky SA, Lin LL (2008) Innate immune responses to TREM-1 activation: overlap, divergence, and positive and negative cross-talk with bacterial lipopolysaccharide. J Immunol 180: 3520–3534.K. DowerDK EllisK. SarafSA JelinskyLL Lin2008Innate immune responses to TREM-1 activation: overlap, divergence, and positive and negative cross-talk with bacterial lipopolysaccharide.J Immunol18035203534
- 60. Gomez-Pina V, Soares-Schanoski A, Rodriguez-Rojas A, Del Fresno C, Garcia F, et al. (2007) Metalloproteinases shed TREM-1 ectodomain from lipopolysaccharide-stimulated human monocytes. J Immunol 179: 4065–4073.V. Gomez-PinaA. Soares-SchanoskiA. Rodriguez-RojasC. Del FresnoF. Garcia2007Metalloproteinases shed TREM-1 ectodomain from lipopolysaccharide-stimulated human monocytes.J Immunol17940654073
- 61. Tessarz AS, Cerwenka A (2008) The TREM-1/DAP12 pathway. Immunol Lett 116: 111–116.AS TessarzA. Cerwenka2008The TREM-1/DAP12 pathway.Immunol Lett116111116
- 62. Boyle JJ, Weissberg PL, Bennett MR (2003) Tumor necrosis factor-alpha promotes macrophage-induced vascular smooth muscle cell apoptosis by direct and autocrine mechanisms. Arterioscler Thromb Vasc Biol 23: 1553–1558.JJ BoylePL WeissbergMR Bennett2003Tumor necrosis factor-alpha promotes macrophage-induced vascular smooth muscle cell apoptosis by direct and autocrine mechanisms.Arterioscler Thromb Vasc Biol2315531558
- 63. Ciesielski-Treska J, Ulrich G, Chasserot-Golaz S, Zwiller J, Revel MO, et al. (2001) Mechanisms underlying neuronal death induced by chromogranin A-activated microglia. J Biol Chem 276: 13113–13120.J. Ciesielski-TreskaG. UlrichS. Chasserot-GolazJ. ZwillerMO Revel2001Mechanisms underlying neuronal death induced by chromogranin A-activated microglia.J Biol Chem2761311313120
- 64. Meszaros AJ, Reichner JS, Albina JE (2000) Macrophage-induced neutrophil apoptosis. J Immunol 165: 435–441.AJ MeszarosJS ReichnerJE Albina2000Macrophage-induced neutrophil apoptosis.J Immunol165435441
- 65. Chen H, Zhang SM, Hernan MA, Schwarzschild MA, Willett WC, et al. (2003) Nonsteroidal anti-inflammatory drugs and the risk of Parkinson disease. Arch Neurol 60: 1059–1064.H. ChenSM ZhangMA HernanMA SchwarzschildWC Willett2003Nonsteroidal anti-inflammatory drugs and the risk of Parkinson disease.Arch Neurol6010591064
- 66. McGeer PL, McGeer EG (2001) Polymorphisms in inflammatory genes and the risk of Alzheimer disease. Arch Neurol 58: 1790–1792.PL McGeerEG McGeer2001Polymorphisms in inflammatory genes and the risk of Alzheimer disease.Arch Neurol5817901792
- 67. Ramesh G, Alvarez AL, Roberts ED, Dennis VA, Lasater BL, et al. (2003) Pathogenesis of Lyme neuroborreliosis: Borrelia burgdorferi lipoproteins induce both proliferation and apoptosis in rhesus monkey astrocytes. Eur J Immunol 33: 2539–2550.G. RameshAL AlvarezED RobertsVA DennisBL Lasater2003Pathogenesis of Lyme neuroborreliosis: Borrelia burgdorferi lipoproteins induce both proliferation and apoptosis in rhesus monkey astrocytes.Eur J Immunol3325392550
- 68. Brandt ME, Riley BS, Radolf JD, Norgard MV (1990) Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins. Infect Immun 58: 983–991.ME BrandtBS RileyJD RadolfMV Norgard1990Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins.Infect Immun58983991
- 69. Cardona AE, Gonzalez PA, Teale JM (2003) CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response. Infect Immun 71: 2634–2642.AE CardonaPA GonzalezJM Teale2003CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.Infect Immun7126342642
- 70. Glabinski AR, Bielecki B, Ransohoff RM (2003) Chemokine upregulation follows cytokine expression in chronic relapsing experimental autoimmune encephalomyelitis. Scand J Immunol 58: 81–88.AR GlabinskiB. BieleckiRM Ransohoff2003Chemokine upregulation follows cytokine expression in chronic relapsing experimental autoimmune encephalomyelitis.Scand J Immunol588188
- 71. Dogan RN, Elhofy A, Karpus WJ (2008) Production of CCL2 by central nervous system cells regulates development of murine experimental autoimmune encephalomyelitis through the recruitment of TNF- and iNOS-expressing macrophages and myeloid dendritic cells. J Immunol 180: 7376–7384.RN DoganA. ElhofyWJ Karpus2008Production of CCL2 by central nervous system cells regulates development of murine experimental autoimmune encephalomyelitis through the recruitment of TNF- and iNOS-expressing macrophages and myeloid dendritic cells.J Immunol18073767384
- 72. Blanco-Aparicio C, Renner O, Leal JF, Carnero A (2007) PTEN, more than the AKT pathway. Carcinogenesis 28: 1379–1386.C. Blanco-AparicioO. RennerJF LealA. Carnero2007PTEN, more than the AKT pathway.Carcinogenesis2813791386
- 73. Heron-Milhavet L, Franckhauser C, Rana V, Berthenet C, Fisher D, et al. (2006) Only Akt1 is required for proliferation, while Akt2 promotes cell cycle exit through p21 binding. Mol Cell Biol 26: 8267–8280.L. Heron-MilhavetC. FranckhauserV. RanaC. BerthenetD. Fisher2006Only Akt1 is required for proliferation, while Akt2 promotes cell cycle exit through p21 binding.Mol Cell Biol2682678280
- 74. Liu X, Shi Y, Han EK, Chen Z, Rosenberg SH, et al. (2001) Downregulation of Akt1 inhibits anchorage-independent cell growth and induces apoptosis in cancer cells. Neoplasia 3: 278–286.X. LiuY. ShiEK HanZ. ChenSH Rosenberg2001Downregulation of Akt1 inhibits anchorage-independent cell growth and induces apoptosis in cancer cells.Neoplasia3278286
- 75. Billen LP, Kokoski CL, Lovell JF, Leber B, Andrews DW (2008) Bcl-XL inhibits membrane permeabilization by competing with Bax. PLoS Biol 6: e147.LP BillenCL KokoskiJF LovellB. LeberDW Andrews2008Bcl-XL inhibits membrane permeabilization by competing with Bax.PLoS Biol6e147
- 76. Feng Y, Zhang L, Hu T, Shen X, Ding J, et al. (2009) A conserved hydrophobic core at Bcl-x(L) mediates its structural stability and binding affinity with BH3-domain peptide of pro-apoptotic protein. Arch Biochem Biophys. Y. FengL. ZhangT. HuX. ShenJ. Ding2009A conserved hydrophobic core at Bcl-x(L) mediates its structural stability and binding affinity with BH3-domain peptide of pro-apoptotic protein.Arch Biochem Biophys
- 77. Hsu YT, Wolter KG, Youle RJ (1997) Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis. Proc Natl Acad Sci U S A 94: 3668–3672.YT HsuKG WolterRJ Youle1997Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis.Proc Natl Acad Sci U S A9436683672
- 78. Minn AJ, Kettlun CS, Liang H, Kelekar A, Vander Heiden MG, et al. (1999) Bcl-xL regulates apoptosis by heterodimerization-dependent and -independent mechanisms. Embo J 18: 632–643.AJ MinnCS KettlunH. LiangA. KelekarMG Vander Heiden1999Bcl-xL regulates apoptosis by heterodimerization-dependent and -independent mechanisms.Embo J18632643
- 79. Crighton D, O'Prey J, Bell HS, Ryan KM (2007) p73 regulates DRAM-independent autophagy that does not contribute to programmed cell death. Cell Death Differ 14: 1071–1079.D. CrightonJ. O'PreyHS BellKM Ryan2007p73 regulates DRAM-independent autophagy that does not contribute to programmed cell death.Cell Death Differ1410711079
- 80. Crighton D, Wilkinson S, Ryan KM (2007) DRAM links autophagy to p53 and programmed cell death. Autophagy 3: 72–74.D. CrightonS. WilkinsonKM Ryan2007DRAM links autophagy to p53 and programmed cell death.Autophagy37274
- 81. Turcotte S, Chan DA, Sutphin PD, Hay MP, Denny WA, et al. (2008) A molecule targeting VHL-deficient renal cell carcinoma that induces autophagy. Cancer Cell 14: 90–102.S. TurcotteDA ChanPD SutphinMP HayWA Denny2008A molecule targeting VHL-deficient renal cell carcinoma that induces autophagy.Cancer Cell1490102
- 82. Albitar L, Carter MB, Davies S, Leslie KK (2007) Consequences of the loss of p53, RB1, and PTEN: relationship to gefitinib resistance in endometrial cancer. Gynecol Oncol 106: 94–104.L. AlbitarMB CarterS. DaviesKK Leslie2007Consequences of the loss of p53, RB1, and PTEN: relationship to gefitinib resistance in endometrial cancer.Gynecol Oncol10694104
- 83. Yamada KM, Araki M (2001) Tumor suppressor PTEN: modulator of cell signaling, growth, migration and apoptosis. J Cell Sci 114: 2375–2382.KM YamadaM. Araki2001Tumor suppressor PTEN: modulator of cell signaling, growth, migration and apoptosis.J Cell Sci11423752382
- 84. Agami R, Bernards R (2000) Distinct initiation and maintenance mechanisms cooperate to induce G1 cell cycle arrest in response to DNA damage. Cell 102: 55–66.R. AgamiR. Bernards2000Distinct initiation and maintenance mechanisms cooperate to induce G1 cell cycle arrest in response to DNA damage.Cell1025566
- 85. Corroyer S, Maitre B, Cazals V, Clement A (1996) Altered regulation of G1 cyclins in oxidant-induced growth arrest of lung alveolar epithelial cells. Accumulation of inactive cyclin E-DCK2 complexes. J Biol Chem 271: 25117–25125.S. CorroyerB. MaitreV. CazalsA. Clement1996Altered regulation of G1 cyclins in oxidant-induced growth arrest of lung alveolar epithelial cells. Accumulation of inactive cyclin E-DCK2 complexes.J Biol Chem2712511725125
- 86. Satyanarayana A, Hilton MB, Kaldis P (2008) p21 Inhibits Cdk1 in the absence of Cdk2 to maintain the G1/S phase DNA damage checkpoint. Mol Biol Cell 19: 65–77.A. SatyanarayanaMB HiltonP. Kaldis2008p21 Inhibits Cdk1 in the absence of Cdk2 to maintain the G1/S phase DNA damage checkpoint.Mol Biol Cell196577
- 87. Armstrong JL, Veal GJ, Redfern CP, Lovat PE (2007) Role of Noxa in p53-independent fenretinide-induced apoptosis of neuroectodermal tumours. Apoptosis 12: 613–622.JL ArmstrongGJ VealCP RedfernPE Lovat2007Role of Noxa in p53-independent fenretinide-induced apoptosis of neuroectodermal tumours.Apoptosis12613622
- 88. Henshall DC, Araki T, Schindler CK, Lan JQ, Tiekoter KL, et al. (2002) Activation of Bcl-2-associated death protein and counter-response of Akt within cell populations during seizure-induced neuronal death. J Neurosci 22: 8458–8465.DC HenshallT. ArakiCK SchindlerJQ LanKL Tiekoter2002Activation of Bcl-2-associated death protein and counter-response of Akt within cell populations during seizure-induced neuronal death.J Neurosci2284588465
- 89. Nechushtan A, Smith CL, Lamensdorf I, Yoon SH, Youle RJ (2001) Bax and Bak coalesce into novel mitochondria-associated clusters during apoptosis. J Cell Biol 153: 1265–1276.A. NechushtanCL SmithI. LamensdorfSH YoonRJ Youle2001Bax and Bak coalesce into novel mitochondria-associated clusters during apoptosis.J Cell Biol15312651276
- 90. Huang DC, Hahne M, Schroeter M, Frei K, Fontana A, et al. (1999) Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x(L). Proc Natl Acad Sci U S A 96: 14871–14876.DC HuangM. HahneM. SchroeterK. FreiA. Fontana1999Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x(L).Proc Natl Acad Sci U S A961487114876