Helicobacter pylori are gram-negative bacteria notable for their high level of genetic diversity and plasticity, features that may play a key role in the organism's ability to colonize the human stomach. Homeologous natural transformation, a key contributor to genomic diversification, has been well-described for H. pylori. To examine the mechanisms involved, we performed restriction analysis and sequencing of recombination products to characterize the length, fragmentation, and position of DNA imported via natural transformation. Our analysis revealed DNA imports of small size (1,300 bp, 95% confidence limits 950–1850 bp) with instances of substantial asymmetry in relation to selectable antibiotic-resistance markers. We also observed clustering of imported DNA endpoints, suggesting a possible role for restriction endonucleases in limiting recombination length. Additionally, we observed gaps in integrated DNA and found evidence suggesting that these gaps are the result of two or more separate strand invasions. Taken together, these observations support a system of highly efficient short-fragment recombination involving multiple recombination events within a single locus.
Helicobacter pylori are gram-negative bacteria that have been implicated in human diseases after decades of persistence in the stomach. Known for its high level of genetic diversity, H. pylori is competent to undergo natural transformation, a process in which donor DNA is integrated into the recipient chromosome. To examine the mechanisms involved, we analyzed the DNA imported via natural transformation in an experimental model system. We found variation in the average length of imported DNA fragments, with asymmetry with respect to a selectable marker. We also found evidence that strain-specific restriction endonucleases may limit recombination length. Additionally, we observed gaps in the integrated DNA and provide evidence that these gaps are the result of separate strand invasions. Together, our observations support a highly efficient system of short-fragment recombination involving multiple recombination events within a small region of the chromosome. This helps explain how bacteria are able to employ genetic recombination to efficiently generate and maintain genomic diversification within the population—a feature that helps H. pylori persistently colonize the harsh environment of the human stomach.
Citation: Lin EA, Zhang X-S, Levine SM, Gill SR, Falush D, Blaser MJ (2009) Natural Transformation of Helicobacter pylori Involves the Integration of Short DNA Fragments Interrupted by Gaps of Variable Size. PLoS Pathog 5(3): e1000337. https://doi.org/10.1371/journal.ppat.1000337
Editor: Steven R. Blanke, University of Illinois, United States of America
Received: June 17, 2008; Accepted: February 12, 2009; Published: March 13, 2009
Copyright: © 2009 Lin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grant RO1 GM63270 from the National Institutes of Health (http://grants.nih.gov/grants/oer.htm). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Many of the bacterial species that are long-term colonizers of human niches are characterized by high levels of genetic diversity . The gram-negative bacteria Helicobacter pylori, which can induce chronic gastritis, peptic ulceration and gastric malignancies after decades of persistence in the human stomach, displays an exceptional level of genomic diversity . Multiple genetically distinguishable isolates have been observed in a single individual, and it has been postulated that genetic diversity and plasticity greatly facilitate adaptation within a host as well as transmission to future hosts –. The finding of highly efficient in vitro recombination, along with evidence of genetic shuffling and a panmictic population structure, suggest that horizontal genetic exchange amongst diverse strains plays a substantial role in generating this extensive gene pool ,.
Most wild-type strains of H. pylori are naturally competent for transformation, which occurs via the actions of proteins encoded by the comB locus –. Horizontal transfer of donor chromosomal DNA containing a point mutation conferring a selectable antibiotic resistance phenotype can be observed in the homeologous natural transformation of H. pylori . The genome of the transformed strain includes a point mutation flanked by varying lengths of the donor DNA. Better knowledge of the characteristics of homeologous recombination may aid in understanding how H. pylori persists through changing environments within the human host, as well as serving as a model for other naturally competent organisms.
Prior studies, which involved mathematical analysis of H. pylori genomic sequence data, provide evidence that the median size of imported donor fragments appears small (412 bp) compared with Streptococcus pneumoniae, Neisseria meningitidis, and Bacillus subtilis (2000, 5000, and 10,000 bp, respectively) –. In addition, as little as 5 bp flanking an antibiotic resistance-conferring point mutation and 150 bp flanking an antibiotic cassette are sufficient to allow transformation to antibiotic resistance ,. In this study, we present a new approach to studying the products of transformation by taking advantage of the naturally-occurring polymorphisms between two sequenced H. pylori strains. We employed restriction analysis and direct sequencing of recombination products to define the length of an imported sequence flanking a point mutation conferring antibiotic-resistance. Using this approach, we were also able to characterize the fragmentation and configuration of this imported DNA. Additionally, we examined whether our findings could be explained by self-hybridization, methylation, or restriction modification of the recombination substrates. Finally, we were able to confirm and compare the transformation characteristics involving more than one genomic locus.
Transformation of strain J99 with 26695 StrR chromosomal DNA
Under the standardized conditions employed , chromosomal DNA from 26695 StrR cells transformed J99 wild-type cells to StrR with a mean frequency of 4.56×10−5 transformants/CFU/µg DNA (Table 1). These data were consistent with those from previous experiments (, data not shown). This frequency of homologous transformation was approximately 10-fold higher than for homeologous transformation with J99 donor chromosomal DNA . Natural transformation resulted in the incorporation of a point mutation (A128G in codon 43 of rpsL in strain 26695 donor DNA) into the recipient strain J99 genome. Our first goal was to determine the length of DNA flanking the point mutation (conferring streptomycin resistance) that was incorporated into the recipient genome along with the mutation. Since there is a ∼6% genetic variation within conserved ORFs between the two strains , the flanking DNA includes numerous single nucleotide polymorphisms (SNPs), that can differentiate between the two strains as the source of a mosaic DNA. For the 8790 bp flanking the StrR site, there were 627 SNPs (7.1% polymorphism) between the strains (mean separation 20.3±19.3 bp). Those SNPs that were within one of the pre-designated restriction sites (Figure 1) were identified.
The map includes restriction sites unique to one or the other strain and the distance (bp) upstream (−) or downstream (+) from the StrR site (A128G in HP1197 of strain 26695, and A263G in JHP1120 of strain J99). Four-digit numbers reflect ORF designations in the annotated genome sequences, and the numbers below these reflect ORF length (bp). Colors indicate ORF homology between the two strains. The grey arrows indicate the direction of transcription. “Downstream” and “upstream” refer to the direction of replication from the origin.
In each independent transformant, we sought to determine the presence or absence of such restriction sites at various distances from the StrR mutation. To achieve this, PCR products were amplified using primers flanking the restriction site (Table S1), and the resulting amplicons were treated with the appropriate restriction enzyme. At distances 100 bp and 399 bp downstream of the StrR (A128G) mutation, all transformants from 10 independent transformations of strain J99 contained restriction sites unique to the donor strain, 26695 (“downstream” or “upstream,” refers to the direction of replication from the origin). However, at 811 bp, 3331 bp, 4668 bp, and 5149 bp downstream of A128G, the number of independent transformants containing the 26695-unique sites fell to 5, 2, 1, and 0, respectively. At a distance of 172 bp upstream of A128G, 9 of the 10 independent transformants analyzed contained the donor 26695-unique sites. That frequency fell to 5, 4 and 0 transformants at increasing distances upstream of A128G (520, 834, and 1350 bp, respectively). No two of the 10 studied transformations yielded identical results, confirming that these transformants were truly independent. The presence or absence of a restriction site was unambiguous within any of the independent transformants, making it unlikely that mixed clones were present.
For each independent transformant, the restriction sites furthest upstream and downstream from StrR that were still unique to 26695 (donor) were identified as proximal to the crossover endpoint. To discriminate the crossover endpoints with greater precision, PCR products were amplified using primers flanking these restriction sites, and sequences of the amplicons were determined. For each, the sequence data revealed a region that was bounded by two SNPs—one unique to 26695 and the other, to J99. To calculate flanking DNA length, the crossover endpoint was defined as the middle of this region. For the 10 independent transformants, the lengths of flanking DNA ranged from 915–4967 bp (Table 1). In total, we observed the mean integration of 2058±1494 bp donor DNA into the recipient chromosome. Despite substantial variation among flanking DNA lengths, two independent transformants were found to contain crossover endpoints within a single 15 bp region (transformants 5 and 6 showed crossovers 505–520 bp downstream of StrR) (Figure 2A).
The values on either side of the bars indicate the distance (bp) from the StrR mutation to two single nucleotide polymorphisms (SNPs), reflecting differential susceptibility to restriction endonucleases that define the recombination endpoints. The endpoint of each DNA segment was designated as the position halfway between the two SNPs. The recombination endpoints were determined by sequencing ∼1 kb near ends indicated by restriction analysis of PCR amplicons. (A) Donor DNA from StrR strain 26695 → Recipient J99 cells. The point mutation in 26695 gene HP1197 conferring streptomycin resistance (A128G) was present in all ten independent transformants; an alternate streptomycin resistance mutation (A263G) present in naturally StrR J99 was not observed. (B) Donor DNA from StrR strain J99 → Recipient 26695 cells. The point mutation in JHP1121 conferring streptomycin resistance (A263G) was present in all ten independent transformants; an alternate streptomycin resistance mutation (A128G) present in naturally StrR 26695 was not observed. For transformants 11,12,14,15,16, and 17, the 8517 bp region (from 2278 bp upstream to 6239 bp downstream) that flanks the StrR mutation was subjected to sequence analysis to completion.
Further analysis of transformant 4 revealed a 13 bp region (4742–4754 bp downstream of the StrR mutation) that can be characterized as a gap in integration. This region contained a SNP unique to J99 (recipient) that was flanked by 2 SNPs unique to 26695 (donor). This result was observed in sequences obtained with both the forward and reverse primers.
The integration of DNA was asymmetric with respect to StrR, favoring the downstream side at a ratio of about 2∶1. Several transformants had integrated flanking DNA with extreme asymmetry, such as transformant 4, which was characterized by a downstream∶upstream ratio of 24∶1.
Transformation of strain 26695 with J99 StrR chromosomal DNA
To examine the hypothesis that flanking DNA asymmetry is influenced by a region of non-homology between 26695 and J99 occurring ∼3 kb upstream of A128G (Figure 1), we repeated the transformation experiment but reversed the roles of the strains. Chromosomal DNA from J99 StrR cells transformed 26695 wild-type cells to StrR with an average efficiency of 1.67×10−6 transformants /CFU/µg DNA (Table 1). Natural transformation resulted in the incorporation of a point mutation (A263G in codon 88 of rpsL in strain J99) into the recipient strain 26695 genome. Surprisingly, at a distance as small as 110 bp downstream of StrR, 5 of 10 transformants contained a restriction site unique to recipient strain 26695, indicating that the contiguous downstream crossover point was very close to the A263G transforming point mutation. Sequence data confirmed this extreme asymmetry with some of the crossover endpoints located only 53 bp downstream of A263G (Figure 2B). Several transformants had very high upstream∶downstream flanking DNA ratios; for example, transformant 14 displayed a ratio of 51∶1.
An average of 2814±1542 bp of flanking DNA was integrated along with the point mutation, with flanking DNA lengths ranging from 634 to 6089 bp. The sequence data for transformants 13 and 15 revealed large gaps of integration, in which the flanking donor DNA is interrupted by significant regions (up to 1658 bp) of recipient DNA (Figure 2B). To examine the flanking DNA for additional gaps, DNA from six independent transformants (11,12,14,15,16, and 17) was completely sequenced within a ∼8.5 kb region from 2278 bp upstream to 6239 bp downstream of the StrR mutation. The sequence data confirmed the identity of the polymorphisms that had been deduced by the previous restriction site analysis (Figure 2B). No additional gaps of integration were observed. Thus, out of 20 independent transformants, only 3 were found to contain gaps (transformants 4, 13, and 15).
Although several transformants had flanking DNA characterized by extreme asymmetry, across the 10 independent transformants, the mean sizes of the flanking DNA fragments upstream and downstream from A263G were similar (1472 bp±965 upstream and 1341 bp±1378 downstream). The flanking DNA of transformant 11 extends upstream by nearly 3 kb and borders the region of non-homology between the two strains. Although no significant difference in total flanking DNA length was found between the two strains, recipient strain 26695 contained significantly more upstream flanking DNA than J99 (Table 1). This is not unexpected since the region of non-homology between the two strains lies upstream in relation to the StrR mutation.
Statistical analysis of import size
To estimate the mean size of imports caused by transformation of chromosomal H. pylori DNA, we performed statistical analysis that takes into account both the uncertainty in the position of the recombination endpoints due to the stretches of sequence identity between J99 and 26695 and the fact that longer imports are more likely to appear in the dataset because they are more likely to include the StrR mutation used to select for strains that have undergone recombination (see Methods). The analysis does not take into account the gaps in integration described above, which are difficult to model statistically. Instead, in a first analysis, we only took into account the stretch of contiguous imported DNA immediately flanking the StrR mutation. This analysis gave a mean import size of 1100 bp (95% confidence limits 800–1600 bp). A second analysis, treating the whole imported stretch as a single unit, and ignoring the effect on ascertainment of the gaps in integration, yields an estimate for the mean size of the entire region involved in the import of 1300 bp (95% confidence limits 950–1850 bp).
Chromosomal markers of enhanced recombination
That no two of the transformants yielded identical results, confirms the independence of each event. However, despite significant variation among flanking DNA lengths, two transformants were found to contain crossover endpoints within a single 15 bp region (transformants 5 and 6 downstream of StrR). Two other transformants (14 and 20) shared an endpoint within 24 bp. Still other transformants (17 and 20) contained crossover endpoints within a single 66 bp region downstream of StrR. Indeed, the endpoints of many transformants were found to be clustered within short regions—transformants 13, 15, and 16 were within a 67 bp region; transformants 14 and 15 were within a 97 bp region; transformants 12 and 15 were within a 93 bp region; transformants 1 and 9 were within a 93 bp region; transformants 15 and 19 were within a 105 bp region; transformants 2 and 5 were within a 105 bp region; and transformants 3 and 7 were within a 108 bp region. Of 48 crossover points in the 20 independent transformants studied in the two experimental protocols, 20 (41.7%) recurred within short regions (<110 bp). These short regions did not coincide with regions of putative self-hybridization, as determined by MFold .
H. pylori strains contain numerous functional methyltransferases that protect endogenous DNA from endonuclease digestion by methylating specific recognition sequences . Methylated genomic DNA may interrupt donor DNA integration during recombination, generating multiple crossover endpoints within a single short region. To explore the hypothesis that genomic DNA methylation patterns play a role in determining crossover endpoints during homeologous transformation, the locations of the methyltransferase recognition sequences in both 26695 and J99 were mapped alongside the crossover endpoints in the 20 independent transformants. This comparison did not yield any significant correlations (data not shown), suggesting that methylation of neither the recipient chromosome nor the donor DNA significantly interrupts the integration of donor DNA.
H. pylori strains 26695 and J99 each encode four different active restriction-modification (R-M) systems . Each system consists of an active restriction endonuclease (RE) and a methytransferase, both recognizing the same sequence. Since the R-M systems are not conserved between the two strains, the donor DNA in homeologous transformation is susceptible to cleavage by REs active in the recipient strain. To examine the role of endonuclease restriction of donor DNA in homeologous transformation, the locations of donor DNA recognition sequences targeted by active recipient strain REs, for which the corresponding methyltransferase is either absent or non-functional in the donor strain, were mapped alongside the crossover endpoints in the 20 transformants (Figure 3). In total, 11 of 23 (48%) crossover endpoints were within regions that overlapped with the specific RE recognition sites. To determine whether this was a chance observation, we performed statistical inference modeling, considering the endpoints of the contiguous imported fragment flanking StrR as being γ times more likely to occur at sites that are susceptible to cleavage due to restriction modification than at sites that are not. This analysis yielded an estimate of γ of 32. However the confidence limits are wide, ranging from 0 to 100, so that based on these 20 events, we have insufficient power to determine whether the restriction sites are significantly overrepresented at import boundaries.
Each panel is a representation of ∼2500 bp flanking a StrR site in strain J99 or 26695. Each horizontal bar represents a region that contains at least two recombination endpoints: a, two endpoints within 100 bp; b, two endpoints within 24 bp; c, three endpoints within 68 bp; d, two endpoints within 70 bp; e, two endpoints within 200 bp; f, two endpoints within 15 bp; g, four endpoints within 100 bp. (A) Map of sites in chromosomal DNA from strain J99 recognized by active 26695 restriction endonucleases for which the corresponding methylase is absent in J99. HpyAII recognition sites (GAAGA) are designated by filled squares (■). HpyAV recognition sites (CCTTC) are designated by filled triangles (▲). (B) Map of sites in 26695 chromosomal DNA recognized by active J99 restriction endonucleases for which the corresponding methylase is absent in 26695. Hpy99IV recognition sites (CCNNGG) are designated by filled circles (●). Hpy99I recognition sites (CGWCG) are designated by filled diamonds (◆).
Transformation of 26695 with PCR products containing one or two antibiotic-resistance alleles
The observation of gaps in integration has been described previously in Streptococcus pneumoniae, and has been attributed to the activity of mismatch repair (MMR) proteins . However, H. pylori does not contain homologs to known MMR genes ,, and has no MMR activity . Since the efficiency of natural transformation was on the order of 10−6, the predicted frequency of adjacent independent recombination events would be ∼10−12. Therefore, we hypothesized that these gaps are the result of DNA repair enzyme activities, rather than adjacent independent recombinations. To examine this hypothesis, we analyzed natural transformation of 26695 cells with a ∼9 kb PCR product (designated P-StrRif) amplified via primers flanking the A128G mutation and a point mutation conferring rifampin-resistance (G1588A in rpoB, resulting in D530V). The mutation conferring RifR is separated from the mutation conferring StrR by ∼7.2 kb (Figure 1). Such transformation yielded StrR transformants and RifR transformants with a frequency of ∼10−4 transformants/CFU/ugDNA, but StrRRifR transformants (containing both mutations) with a frequency of 4.7×10−6 transformants/CFU/ug DNA (Table 2). These findings suggest a linkage between the two markers. However, since both markers are co-localized on a single PCR product, it is not clear whether this linkage was the result of a single DNA crossover event or multiple ones. To differentiate between these hypotheses, we also transformed 26695 cells with an equimolar mixture of the ∼9 kb PCR products containing either the StrR mutation (designated P-Str) or the RifR mutation (designated P-Rif). Under these conditions, transformation efficiencies were similar to those from the single product (Table 2). The frequency of finding an integrated RifR mutation adjacent to a given StrR mutation (or visa versa) was ∼1/130. That this frequency is several log10 higher than the frequency predicted for two adjacent independent recombinations, suggests that the integration of both markers is mechanistically linked, and that such linkage occurs even if both markers are located on separate DNA molecules.
To further explore this linkage, we repeated the experiment using J99 cells and a donor ∼9 kb PCR product derived from a 26695 StrR RifR template. Using restriction analysis (as described above), transformant chromosomal DNA was analyzed for the presence of a SNP unique to J99 (recipient) that was approximately equidistant from the StrR and RifR mutations. Such analysis confirmed the presence of the SNP in 6 of 6 transformants, suggesting that within the transformant DNA, a gap in integration exists between the two antibiotic resistance mutations. The presence of such a gap is consistent with a mechanism involving multiple crossover events. This analysis was also repeated after reversing the strains. 26695 cells were transformed with a donor ∼9 kb PCR product derived from a J99 StrRRifR template, yielding StrRRifR transformants which were analyzed for the same SNP. This analysis found that four of the six independent transformants contained the SNP unique to 26695 (recipient), but two transformants had the donor SNP. While this finding does not rule out the presence of a gap in integration between the StrR and RifR mutations in these two transformants, it suggests that multiple mechanisms may be involved in the generation of these genetic products during homeologous transformation.
Transformation involving an alternate locus
The rpsL gene encodes an abundant ribosomal binding protein, and is located within a highly transcribed region of the genome. To evaluate the products of natural transformation in other less-highly transcribed genomic regions, we studied the region of recG. Previous data involving microarrays have shown that the transcription rate of recG is 15 to 87-fold less than that of rpsL . For our studies, we used the pRecGCat plasmid, as described previously . pRecGCat contains a sequence corresponding to recG of strain J99 that is interrupted by a chloramphenicol acetyl transferase (cat) cassette conferring chloramphenicol resistance (CmR). In initial studies, we transformed pRecGCat into wild-type J99 cells to generate strain HPXZ136. Chromosomal DNA from this strain then was used to transform 26695 cells; 10 independent transformants were isolated, as in the prior experiments. The frequency of transformation was 3.0×10−6±2.5×10−6 transformants/CFU/µg DNA, which is ∼10-fold less than for the transformations involving the StrR point mutation. The transformation frequency for the control experiment (J99 recG cat→J99) was similar (6.3×10−6±3.1×10−6 transformants/CFU/µg DNA), indicating no large restriction barrier for this transformation into 26695. As in the prior experiments (see Materials and Methods), we performed sequence analysis on 10 independent transformants, yielding the locations of the upstream and downstream crossover endpoints (Figure 4A). In total, in addition to the selectable 884 bp cat cassette, the mean flanking insert size was 3393±1013 bp (range 1650–4355 bp). The average lengths of the upstream (1771±936 bp) and downstream (1623±959 bp) inserted sequences were similar, although the actual fragment lengths varied considerably. This is >1 kb more than the mean length of integrated DNA observed for the StrR mutation. No gaps in integration were observed within any of the 10 transformants, nor was there clustering of crossover endpoints.
The values above the bars indicate the distance (bp) from the cat cassette to the recombination endpoints (red arrows), as determined by sequence analysis. The numbers reflect the distance from the nearest cassette boundary; the range reflects the locations of informative SNPs. Sequences corresponding to J99 are colored blue; those corresponding to 26695 are colored yellow. (A) J99-derived donor HPXZ136 transforming 26695 cells. (B) 26695-derived donor HPXZ135 transforming J99 cells.
Strain 26695 contains two active restriction endonucleases (HpyAII and HpyAV) for which J99 (and thus HPXZ136) lacks the cognate methylases. Within the range of the cross-overs, there are 19 upstream and 12 downstream sites on the J99 chromosome that would be cleaved by these 26695 REs. In total, 6 (30%) of the 20 endpoints overlap with the specific restriction sites, a finding significantly greater than chance (p = 0.046).
In a second experiment involving the recG locus, we used a more complex transformation strategy (Figure 4B). We transformed pRecGCat into wild-type 26695 cells to create strain HPXZ135. Sequence analysis of HPXZ135 revealed a ∼200 bp segment of 26695 sequence interrupting the J99 sequence immediately upstream of the cat cassette (Figure 4B). This ∼200 segment was designated as the “donor gap.” We then used chromosomal DNA from HPXZ135 (containing the “donor gap”) to transform wild-type J99 recipient cells. 10 independent transformants were isolated, as in previous experiments. This transformation yielded colonies at a mean efficiency of 5.60×10−6±6.93×10−6 transformants/CFU/µg DNA, similar to the results when HPXZ136 was used. Sequence analysis performed on the 10 independent transformants identified the locations of upstream and downstream crossover endpoints (Figure 4B). In total, we observed a mean integration of 3098±946 bp donor DNA into the recipient chromosome, with similar upstream (1695±795 bp) and downstream (1392±1157 bp) sequence lengths. In each transformant, the architecture of the donor DNA was preserved, with the cat cassette and the “donor gap” present in all transformants (Figure 4B). The transformation fragment variation reflected the location of the crossovers involving the flanking 26695 sequences. As in the prior analysis (Figure 4A), we did not observe any clustering of endpoints.
We mapped the sites of known active J99 restriction endonucleases (Hpy99I, Hpy99II, and Hpy99IV) whose corresponding methylases are absent in 26695 and thus, HPXZ135. We found relatively few RE sites flanking the cat cassette, with only 4 sites within 4 kb downstream of the cassette (708, 2097, 3019, and 3020 bp from the cassette) and none upstream. With so few informative markers, we found no relationship between crossover points and restriction sites.
In S. pneumoniae, N. meningitidis and B. subtilis, transformation yields imported DNA lengths of about 2 kb, 5 kb and 10 kb, respectively –. It might have been anticipated that transformation of H. pylori would result in imported DNA lengths at the upper end of this range since H. pylori lacks homologs to DNA recombination/repair proteins , which have been implicated in limiting the length of heteroduplex formation during recombination in both prokaryotes and eukaryotes . However, natural transformation of H. pylori yields insertion lengths near the smaller portion of this range, with a mean integration of ∼2400 bp of donor chromosomal DNA into the recipient chromosome (Table 1). After statistical treatment of the data, the mean import size was found to be ∼1300 bp, which is consistent with previous reports of small import lengths within naturally occurring mosaics of H. pylori, as well as efficient in vitro transformation with small donor DNA fragments ,. Although it has been suggested that digestion of naked DNA within the external environment may play a role in reducing the length of imported fragments, a significantly greater mean import length was not observed despite limiting the donor substrate to freshly purified chromosomal DNA extract. While excessive mechanical shearing of the donor DNA cannot be excluded, it is generally prevented with standard techniques and would not explain the astochastic distribution of crossover endpoints .
In other naturally competent organisms, such as N. gonorrhoeae, S. pneumoniae, and B. subtilis, DNA is imported as a single strand that is resistant to cleavage by endonucleases –. However, there is evidence to suggest that natural transformation in H. pylori may be different. Natural competence in H. pylori is associated with proteins at the comB locus—a feature not found in other naturally competent bacteria –. Transformation of H. pylori involving double-stranded donor DNA results in ∼1000-fold higher transformation efficiency than transformation with a single-stranded substrate . Furthermore, evidence of a restriction barrier has been found in H. pylori; for example, strain-specific methylation of donor DNA transforms H. pylori more efficiently than unmethylated DNA that is susceptible to restriction –. If substrate donor DNA exists in the cytoplasm as a double-strand, the action of native strain-specific H. pylori restriction endonucleases would be predicted to curtail import size. In our data, we observed an overrepresentation of crossover endpoints at susceptible restriction sites, which would be the expected consequence of donor substrate DNA restriction. It would be interesting to compare our findings with those in strains that do not harbor R-M systems.
The finding of imports with extreme asymmetry with respect to the selective StrR mutation is consistent with the previous observation that H. pylori is successfully transformed by asymmetric donor DNA fragments with minimal homology on one flank and is suggestive of a highly efficient mechanism for recombination . This finding is also consistent with the observation that flanking DNA in one transformant actually abuts the region of non-homology between strains 26695 and J99. This is unsurprising in an organism that lacks MMR enzymes, since such enzymes would be expected to play a role in the early termination of recombination at polymorphic sites.
The observation of gaps of integration has been previously described for S. pneumoniae . In bacterial transformation, initiation of recombination involves the invasion of a single-stranded segment of donor DNA into the recipient chromosome. The finding of gaps of integration has been previously attributed to events that occur following donor strand invasion, such as repair excision of mismatched bases, resulting in the conversion of genetically heterozygous sites to homozygosity ,. However, since H. pylori lacks a full complement of mismatch repair enzymes, we hypothesized that these gaps may be due to events occurring before or during donor strand invasion. For example, the initiation of two separate but adjacent strand invasion events would result in two neighboring regions of donor genotype separated by a gap.
However, the coincidence of two or more independent strand invasion events within ∼3 kb (see Figure 2B) would be unexpected given our sample size, unless the events were mechanistically linked. One example of linkage would involve the origination of both invading strands from a contiguous DNA molecule. However, we found that the simultaneous genomic integration of two genetic markers present on two separate DNA molecules occurred with the same frequency as the integration of markers coexisting on the same molecule (Table 2). While multiple hypotheses exist to account for such linkage, including local concentration effects due to enzyme recruitment, the uptake of large quantities of donor DNA by the cell, and increased susceptibility of certain genomic regions to recombination, these findings suggest that any explanation for the mechanism of linkage between the two markers must include an account for adjacent strand invasions involving at least two discrete DNA molecules. This finding is certainly consistent with a donor substrate that has been cleaved by R-M system enzymes prior to strand invasion of the chromosome. However, the StrR and RifR mutations are located in highly transcribed essential genes, for which the measured recombination rate may differ from that in other parts of the genome.
It might be suggested, that the observation of relatively large gaps within a 9 kb region and the finding of much smaller gaps within a ∼3 kb region (Figure 2B), are the result of two different processes. While the incidence of gaps in the 9 kb region is significantly lower than for the 3 kb region (an incidence of ∼1/130 for the 9 kb region versus 3/20 for the 3 kb region), that may not be unexpected since two simultaneous strand invasions that are far apart may occur much less frequently than strand invasions that are closer together. Furthermore, the finding that the incidence of gaps varies at 9 kb versus 3 kb suggests that location and proximity of markers does indeed play a role, and that the simultaneous integration of both markers is not simply the result of two coincidental but independent events. The finding of significant gaps within the 9 kb region strongly suggests that the distance between the StrR and RifR markers (∼7.2 kb) may be too long for a single co-transformation event. Thus, each doubly marked fragment only transforms at a single site, but not at both. These observations set an upper boundary (of 7.2 kb) for the amount of DNA that can be contiguously integrated from a single site, but may reflect the restriction and methylation differences between the donor and recipient strains.
To examine whether the same recombination characteristics are present for non-essential genes, we transformed wild-type 26695 cells with chromosomal DNA from strain HPXZ136, which contains a cat cassette within the recG gene. Interestingly, the frequencies of transformation between recG and rpsL were similar (Table 1). Given that the transcription rate of recG is up to 87-fold lower than that of rpsL , this suggests that differences in transcription rate may not have a major effect on transformation efficiency. Genomic sequencing of the resulting transformants showed no evidence of gaps in integration or clustering of endpoints, as had been found flanking the StrR mutation in rpsL. However, there was a significant overrepresentation of endpoints that overlapped with sites in which the incoming J99 DNA would be susceptible to 26695-specific restriction. As a further test, we performed a transformation involving a more complex genomic architecture from strain 26695 into J99. The frequency of transformation was almost the same as when the simpler architecture was used, indicating no substantial differences whether or not a “donor gap” in the identity of the sequence was introduced. The lack of endpoint clustering was consistent with the very small number of J99-specific restriction sites on the incoming 26695-based donor DNA. Of note, the distribution of flanking DNA was relatively symmetric with respect to the cat cassette, a finding that contrasts with the asymmetric distribution of DNA flanking the StrR mutation. This may be due to the loss of homology ∼3 kb upstream of the StrR mutation in rpsL, a feature not found in recG.
One potential limitation of our technique is the lack of fully-quantitative standardization of cell density prior to the introduction of donor DNA. However our method of standardizing cell density has yielded highly consistent transformation efficiencies over time, and the transformation frequencies we have observed are consistent with previous experiments .
In conclusion, we have presented a new approach to studying the products of transformation that has yielded evidence of highly efficient short-fragment recombination involving multiple recombination events within a single locus. These events likely involve distinct genetic markers residing on discrete DNA molecules, as is the case following processing by cell-specific restriction endonucleases. While our findings may also be partially explained by the lack of a mismatch repair pathway in H. pylori, it would be worthwhile to consider the role of other proteins that regulate recombination, such as MutS2 . It also would be of interest to consider the role of various type I and type III R-M systems that are present in H. pylori. By phylogeography, 26695 is an hpEurope strain while J99 is of the hpAfrica1 lineage, which suggests that they are not closely related despite commonalities within certain loci (eg. cag and vacA) . It may be interesting to explore whether genetic exchange between more-closely related strains, with fewer R-M system barriers, yields different results. Within this context, we have uncovered novel and interesting insights into how recombination occurs around a selectable genetic marker. This will help us to further understand how bacteria are able to employ genetic recombination to efficiently generate and maintain genomic diversification within the population—a feature that allows H. pylori to persistently colonize the human stomach .
Materials and Methods
Strains and growth conditions
The H. pylori isolates used in this study were wild-type strains 26695 and J99, for which the complete genomic sequences have been solved ,. H. pylori cells were grown at 37°C in 5% CO2 for 48 h on Trypticase soy agar (TSA) with 5% sheep blood (BBL Microbiology Systems, Cockeysville, MD) or Brucella-serum (BS;BBL) agar with 10% newborn calf serum (Serologicals Corporation, Norcross, GA) plates. Spontaneously streptomycin-resistant (StrR) mutants of these strains were selected by plating approximately 1010 cells on TSA medium containing streptomycin (10 µg/ml). This procedure yielded H. pylori strains that had single point mutations conferring streptomycin resistance in rpsL; for 26695, the mutation was A128G (K43R), and for J99, the mutation was A263G (K88R) ,. For the transformation experiments involving donor DNA, independent transformants were selected on BS plates with streptomycin (20 µg/ml) added. Rifampin-resistant (RifR) H. pylori strains were obtained by natural transformation of 26695 cells with PCR products amplified using primers containing an A1589T (D530V) point mutation in rpoB that confers rifampin resistance . Each independent transformant was harvested from a single colony growing on a separate plate, to ensure that each transformant represented an entirely independent genetic clone. A chlorampheniol-resistant (CmR) J99 strain, HPXZ136, was generated by transforming wild-type J99 with plasmid pRecGCat which is based on J99 recG , and then selecting one single colony from a BS plate containing chloramphenicol (10 µg/ml). The cat cassette insertion site in the HPXZ136 genome and its flanking region was confirmed by sequencing. A second transforming DNA was prepared by using pRecGCat (with J99 recG) to transform strain 26695, with the same selection and confirmation as above, creating HPXZ135. This strain has a 200 bp gap of 26695 sequence within the J99 sequence as a result of the pRecGCat transformation.
H. pylori chromosomal DNA was prepared from cells of each independent transformant after 48 h of growth on TSA plates, as described . PCR was performed on the isolated DNA in a reaction volume of 100 µl containing 1U of Taq (Qiagen, Valencia, CA) as per manufacturer's protocol. Each pair of primers was selected to exactly match the chromosomal sequences in both J99 and 26695 (Table S1), and flanked a restriction site unique to that segment for either 26695 or J99 (Figure 1). Restriction enzyme (RE) digestions were performed in a reaction volume of 20 µl containing 2U of the appropriate RE in its buffer at 37°C for 12 h. All PCR products were electrophoresed at 120V for 60 min through 2% agarose gels with 0.7 µg/mL ethidium bromide. The desired band was excised from the gel, purified (Qiagen, Valencia, CA), and stored at −20°C until used in transformation studies.
The H. pylori cells to be transformed were grown on trypticase soy agar (TSA) plates with 5% sheep blood (BBL) agar for 48 h, harvested into 1 ml of phosphate-buffered saline (PBS) pH 7.4, centrifuged at 850 g for 5 min, and the pellet resuspended in 150 µl of PBS. Each transformation mixture, consisting of 25 µl of recipient cells (∼108 cells) and 15 µl (at 2 ng/µl) of donor cell chromosomal DNA, was spotted onto a TSA plate and the plates were incubated for 24 h at 37°C in a 5% CO2 atmosphere. The transformation mixture then was harvested into 1 ml of PBS, and 100-µl aliquots of appropriate serial dilutions inoculated to both TSA (non-selective) and antibiotic (selective) plates with 20 µg/ml streptomycin, 87.5 µg/ml rifampin, or both, and incubated for 5 days at 37°C in a 5% CO2 atmosphere. The number of colonies of transformants and total viable cells were counted and the transformation frequency was calculated as the number of streptomycin-resistant colonies per microgram of DNA per recipient CFU. Transformation using the cat cassette was performed similarly, except that 15 µl (containing 100 ng) of donor chromosomal DNA was used. CmR transformants were selected on BS agar plates with 10 µg/ml chloramphenicol.
Mapping the transforming fragment
For each recipient strain, a single aliquot of cells harvested from a fully confluent plate, was divided into 25 ul portions, which were each placed in the center of a separate TSA plate. Donor DNA was then introduced to each plate and the plates were incubated overnight. The resulting transformed cells from each plate were then harvested, diluted, and inoculated onto an antibiotic plate. One colony from each antibiotic plate was selected with a sterile pipette, inoculated onto a TSA plate, incubated for 4 days, and harvested. Chromosomal DNA was prepared using a CTAB/Chloroform/Phenol extraction, as described . In transformations utilizing donor PCR products, 9 kb PCR amplicons were first generated using primers flanking the StrR mutation (A128G) and a mutation conferring rifampin resistance (G1588A in codon 530 of rpoB, resulting in D530V). Cells of strain 26695 were exposed to either 100 ng of PCR products containing both mutations or an equimolar mixture of two different amplicons (100 ng each), each containing only one mutation. Serial dilutions were inoculated to TSA plates, 20 µg/ml streptomycin plates, 87.5 µg/ml rifampin plates, and to plates containing both streptomycin and rifampin.
Each CmR transformant was picked with a sterile pipette, inoculated onto a separate BS agar plate with 10 µg/ml chloramphenicol, and incubated for 3 days. The culture from the selective plate was swabbed onto a TSA plate, incubated for 2 days, and harvested. Chromosomal DNA was prepared using the Wizard genomic DNA purification kit (Promega, Madison, WI). To analyze the region of DNA flanking the cat cassette, PCR was performed using chromosomal isolates from 10 independent transformants. Primers were designed to match the sequences in both 26695 and J99 (Table S2). The desired PCR products were purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA).
For analysis of specific sequences flanking the StrR mutation, 0.5–1 kb PCR products were prepared as described above and were sequenced on both strands at the DNA Sequencing Resource Center (Rockefeller University, New York, NY). For analysis of specific sequences flanking the cat cassette, ∼1 kb PCR products were prepared as described above and sequenced on both strands at the High-Throughput Genomics Unit (University of Washington, Seattle, WA). All sequence analysis was performed using Sequencher software (Gene Codes, Ann Arbor, MI).
Statistical analysis of imports
If the distribution of imports is assumed to be exponential with mean length λ, and imports are randomly distributed on the chromosome, then the probability density function of imports of length x containing the StrR mutation will be equal to , since the probability that any given import will include the StrR site is proportional to its length. Not all nucleotides differentiate 26695 from J99, so the length of each observed import is ambiguous. If an import has possible start positions ranging from a to b and end positions from c to d then the likelihood of observing it is . The overall likelihood of the data given λ is the arithmetic product of the likelihoods of all imports.
Restriction modification could result in some sites being more likely to contain the endpoints of imports than others. We model this by assuming that unmethylated sites for which the restriction modification system is present in the recipient strain are γ times more likely to be digested than other sites. The likelihood of observing a particular event of length, starting at and finishing at site , conditional on it containing the StrR mutation at site 0 then is proportional to , where if x is at an unmethylated restriction site and otherwise. This expression is approximated by truncating the sum at values of c and d that bound all observed imports ( for imports from 26695 to J99 and for imports from J99 to 26695).
Primers used to assess DNA crossover sites
(0.06 MB DOC)
Conceived and designed the experiments: EAL XSZ SML MJB. Performed the experiments: EAL XSZ SRG. Analyzed the data: EAL XSZ SML DF MJB. Contributed reagents/materials/analysis tools: EAL SRG DF MJB. Wrote the paper: EAL XSZ MJB.
- 1. Krinos CM, Coyne MJ, Weinacht KG, Tzianabos AO, Kasper DL, et al. (2001) Extensive surface diversity of a commensal microorganism by multiple DNA inversions. Nature 414: 555–558.CM KrinosMJ CoyneKG WeinachtAO TzianabosDL Kasper2001Extensive surface diversity of a commensal microorganism by multiple DNA inversions.Nature414555558
- 2. Peek RM Jr, Blaser MJ (2002) Helicobacter pylori and gastrointestinal tract adenocarcinomas. Nat Rev Cancer 2: 28–37.RM Peek JrMJ Blaser2002Helicobacter pylori and gastrointestinal tract adenocarcinomas.Nat Rev Cancer22837
- 3. Israel DA, Salama N, Arnold CN, Moss SF, Ando T, et al. (2001) Helicobacter pylori strain-specific differences in genetic content, identified by microarray, influence host inflammatory responses. J Clin Invest 107: 611–620.DA IsraelN. SalamaCN ArnoldSF MossT. Ando2001Helicobacter pylori strain-specific differences in genetic content, identified by microarray, influence host inflammatory responses.J Clin Invest107611620
- 4. Kuipers EJ, Israel DA, Kusters JG, Gerrits MM, Weel J, et al. (2000) Quasispecies development of Helicobacter pylori observed in paired isolates obtained years apart from the same host. J Infect Dis 181: 273–282.EJ KuipersDA IsraelJG KustersMM GerritsJ. Weel2000Quasispecies development of Helicobacter pylori observed in paired isolates obtained years apart from the same host.J Infect Dis181273282
- 5. van der Ende A, Rauws EA, Feller M, Mulder CJ, Tytgat GN, et al. (1996) Heterogeneous Helicobacter pylori isolates from members of a family with a history of peptic ulcer disease. Gastroenterology 111: 638–647.A. van der EndeEA RauwsM. FellerCJ MulderGN Tytgat1996Heterogeneous Helicobacter pylori isolates from members of a family with a history of peptic ulcer disease.Gastroenterology111638647
- 6. Kersulyte D, Chalkauskas H, Berg DE (1999) Emergence of recombinant strains of Helicobacter pylori during human infection. Mol Microbiol 31: 31–43.D. KersulyteH. ChalkauskasDE Berg1999Emergence of recombinant strains of Helicobacter pylori during human infection.Mol Microbiol313143
- 7. Suerbaum S, Smith JM, Bapumia K, Morelli G, Smith NH, et al. (1998) Free recombination within Helicobacter pylori. Proc Natl Acad Sci U S A 95: 12619–12624.S. SuerbaumJM SmithK. BapumiaG. MorelliNH Smith1998Free recombination within Helicobacter pylori.Proc Natl Acad Sci U S A951261912624
- 8. Levine SM, Lin EA, Emara W, Kang J, DiBenedetto M, et al. (2007) Plastic cells and populations: DNA substrate characteristics in Helicobacter pylori transformation define a flexible but conservative system for genomic variation. Faseb J 21: 3458–3467.SM LevineEA LinW. EmaraJ. KangM. DiBenedetto2007Plastic cells and populations: DNA substrate characteristics in Helicobacter pylori transformation define a flexible but conservative system for genomic variation.Faseb J2134583467
- 9. Israel DA, Lou AS, Blaser MJ (2000) Characteristics of Helicobacter pylori natural transformation. FEMS Microbiol Lett 186: 275–280.DA IsraelAS LouMJ Blaser2000Characteristics of Helicobacter pylori natural transformation.FEMS Microbiol Lett186275280
- 10. Tsuda M, Karita M, Nakazawa T (1993) Genetic transformation in Helicobacter pylori. Microbiol Immunol 37: 85–89.M. TsudaM. KaritaT. Nakazawa1993Genetic transformation in Helicobacter pylori.Microbiol Immunol378589
- 11. Hofreuter D, Karnholz A, Haas R (2003) Topology and membrane interaction of Helicobacter pylori ComB proteins involved in natural transformation competence. Int J Med Microbiol 293: 153–165.D. HofreuterA. KarnholzR. Haas2003Topology and membrane interaction of Helicobacter pylori ComB proteins involved in natural transformation competence.Int J Med Microbiol293153165
- 12. Hofreuter D, Odenbreit S, Henke G, Haas R (1998) Natural competence for DNA transformation in Helicobacter pylori: identification and genetic characterization of the comB locus. Mol Microbiol 28: 1027–1038.D. HofreuterS. OdenbreitG. HenkeR. Haas1998Natural competence for DNA transformation in Helicobacter pylori: identification and genetic characterization of the comB locus.Mol Microbiol2810271038
- 13. Karnholz A, Hoefler C, Odenbreit S, Fischer W, Hofreuter D, et al. (2006) Functional and topological characterization of novel components of the comB DNA transformation competence system in Helicobacter pylori. J Bacteriol 188: 882–893.A. KarnholzC. HoeflerS. OdenbreitW. FischerD. Hofreuter2006Functional and topological characterization of novel components of the comB DNA transformation competence system in Helicobacter pylori.J Bacteriol188882893
- 14. Falush D, Kraft C, Taylor NS, Correa P, Fox JG, et al. (2001) Recombination and mutation during long-term gastric colonization by Helicobacter pylori: estimates of clock rates, recombination size, and minimal age. Proc Natl Acad Sci U S A 98: 15056–15061.D. FalushC. KraftNS TaylorP. CorreaJG Fox2001Recombination and mutation during long-term gastric colonization by Helicobacter pylori: estimates of clock rates, recombination size, and minimal age.Proc Natl Acad Sci U S A981505615061
- 15. Linz B, Schenker M, Zhu P, Achtman M (2000) Frequent interspecific genetic exchange between commensal Neisseriae and Neisseria meningitidis. Mol Microbiol 36: 1049–1058.B. LinzM. SchenkerP. ZhuM. Achtman2000Frequent interspecific genetic exchange between commensal Neisseriae and Neisseria meningitidis.Mol Microbiol3610491058
- 16. Guild WR, Cato A Jr, Lacks S (1968) Transformation and DNA size: two controlling parameters and the efficiency of the single strand intermediate. Cold Spring Harb Symp Quant Biol 33: 643–645.WR GuildA. Cato JrS. Lacks1968Transformation and DNA size: two controlling parameters and the efficiency of the single strand intermediate.Cold Spring Harb Symp Quant Biol33643645
- 17. Fornili SL, Fox MS (1977) Electron microscope visualization of the products of Bacillus subtilis transformation. J Mol Biol 113: 181–191.SL ForniliMS Fox1977Electron microscope visualization of the products of Bacillus subtilis transformation.J Mol Biol113181191
- 18. Pyndiah S, Menard A, Zerbib F, Megraud F (2005) Evaluation of the homologous recombination in Helicobacter pylori. Helicobacter 10: 185–192.S. PyndiahA. MenardF. ZerbibF. Megraud2005Evaluation of the homologous recombination in Helicobacter pylori.Helicobacter10185192
- 19. Alm RA, Ling LS, Moir DT, King BL, Brown ED, et al. (1999) Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397: 176–180.RA AlmLS LingDT MoirBL KingED Brown1999Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori.Nature397176180
- 20. Zuker M, Mathews DH, Turner DH (1999) Algorithms and Thermodynamics for RNA Secondary Structure Prediction: A Practical Guide in RNA Biochemistry and Biotechnology. Kluwer Academic Publishers. M. ZukerDH MathewsDH Turner1999Algorithms and Thermodynamics for RNA Secondary Structure Prediction: A Practical Guide in RNA Biochemistry and Biotechnology.Kluwer Academic Publishers
- 21. Kong H, Lin LF, Porter N, Stickel S, Byrd D, et al. (2000) Functional analysis of putative restriction-modification system genes in the Helicobacter pylori J99 genome. Nucleic Acids Res 28: 3216–3223.H. KongLF LinN. PorterS. StickelD. Byrd2000Functional analysis of putative restriction-modification system genes in the Helicobacter pylori J99 genome.Nucleic Acids Res2832163223
- 22. Lin LF, Posfai J, Roberts RJ, Kong H (2001) Comparative genomics of the restriction-modification systems in Helicobacter pylori. Proc Natl Acad Sci U S A 98: 2740–2745.LF LinJ. PosfaiRJ RobertsH. Kong2001Comparative genomics of the restriction-modification systems in Helicobacter pylori.Proc Natl Acad Sci U S A9827402745
- 23. Guerrini F, Fox MS (1968) Effects of DNA repair in transformation-heterozygotes of pneumococcus. Proc Natl Acad Sci U S A 59: 1116–1123.F. GuerriniMS Fox1968Effects of DNA repair in transformation-heterozygotes of pneumococcus.Proc Natl Acad Sci U S A5911161123
- 24. Tomb JF, White O, Kerlavage AR, Clayton RA, Sutton GG, et al. (1997) The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388: 539–547.JF TombO. WhiteAR KerlavageRA ClaytonGG Sutton1997The complete genome sequence of the gastric pathogen Helicobacter pylori.Nature388539547
- 25. Kang J, Huang S, Blaser MJ (2005) Structural and functional divergence of MutS2 from bacterial MutS1 and eukaryotic MSH4-MSH5 homologs. J Bacteriol 187: 3528–3537.J. KangS. HuangMJ Blaser2005Structural and functional divergence of MutS2 from bacterial MutS1 and eukaryotic MSH4-MSH5 homologs.J Bacteriol18735283537
- 26. Loh JT, Torres VJ, Cover TL (2007) Regulation of Helicobacter pylori cagA expression in response to salt. Cancer Res 67: 4709–4715.JT LohVJ TorresTL Cover2007Regulation of Helicobacter pylori cagA expression in response to salt.Cancer Res6747094715
- 27. Kang J, Blaser MJ (2008) Repair and antirepair DNA helicases in Helicobacter pylori. J Bacteriol 190: 4218–4224.J. KangMJ Blaser2008Repair and antirepair DNA helicases in Helicobacter pylori.J Bacteriol19042184224
- 28. Kang J, Blaser MJ (2006) Bacterial populations as perfect gases: genomic integrity and diversification tensions in Helicobacter pylori. Nat Rev Microbiol 4: 826–836.J. KangMJ Blaser2006Bacterial populations as perfect gases: genomic integrity and diversification tensions in Helicobacter pylori.Nat Rev Microbiol4826836
- 29. Chen W, Jinks-Robertson S (1998) Mismatch repair proteins regulate heteroduplex formation during mitotic recombination in yeast. Mol Cell Biol 18: 6525–6537.W. ChenS. Jinks-Robertson1998Mismatch repair proteins regulate heteroduplex formation during mitotic recombination in yeast.Mol Cell Biol1865256537
- 30. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, et al. (1989) Current protocols in molecular biology. New York: Greene Pub. Associates and Wiley-Interscience (J. Wiley). FM AusubelR. BrentRE KingstonDD MooreJG Seidman1989Current protocols in molecular biologyNew YorkGreene Pub. Associates and Wiley-Interscience (J. Wiley)
- 31. Dubnau D (1999) DNA uptake in bacteria. Annu Rev Microbiol 53: 217–244.D. Dubnau1999DNA uptake in bacteria.Annu Rev Microbiol53217244
- 32. Hamilton HL, Dillard JP (2006) Natural transformation of Neisseria gonorrhoeae: from DNA donation to homologous recombination. Mol Microbiol 59: 376–385.HL HamiltonJP Dillard2006Natural transformation of Neisseria gonorrhoeae: from DNA donation to homologous recombination.Mol Microbiol59376385
- 33. Lorenz MG, Wackernagel W (1994) Bacterial gene transfer by natural genetic transformation in the environment. Microbiol Rev 58: 563–602.MG LorenzW. Wackernagel1994Bacterial gene transfer by natural genetic transformation in the environment.Microbiol Rev58563602
- 34. Ando T, Xu Q, Torres M, Kusugami K, Israel DA, et al. (2000) Restriction-modification system differences in Helicobacter pylori are a barrier to interstrain plasmid transfer. Mol Microbiol 37: 1052–1065.T. AndoQ. XuM. TorresK. KusugamiDA Israel2000Restriction-modification system differences in Helicobacter pylori are a barrier to interstrain plasmid transfer.Mol Microbiol3710521065
- 35. Aras RA, Small AJ, Ando T, Blaser MJ (2002) Helicobacter pylori interstrain restriction-modification diversity prevents genome subversion by chromosomal DNA from competing strains. Nucleic Acids Res 30: 5391–5397.RA ArasAJ SmallT. AndoMJ Blaser2002Helicobacter pylori interstrain restriction-modification diversity prevents genome subversion by chromosomal DNA from competing strains.Nucleic Acids Res3053915397
- 36. Donahue JP, Israel DA, Peek RM, Blaser MJ, Miller GG (2000) Overcoming the restriction barrier to plasmid transformation of Helicobacter pylori. Mol Microbiol 37: 1066–1074.JP DonahueDA IsraelRM PeekMJ BlaserGG Miller2000Overcoming the restriction barrier to plasmid transformation of Helicobacter pylori.Mol Microbiol3710661074
- 37. White RL, Fox MS (1974) On the molecular basis of high negative interference. Proc Natl Acad Sci U S A 71: 1544–1548.RL WhiteMS Fox1974On the molecular basis of high negative interference.Proc Natl Acad Sci U S A7115441548
- 38. Pinto AV, Mathieu A, Marsin S, Veaute X, Ielpi L, et al. (2005) Suppression of homologous and homeologous recombination by the bacterial MutS2 protein. Mol Cell 17: 113–120.AV PintoA. MathieuS. MarsinX. VeauteL. Ielpi2005Suppression of homologous and homeologous recombination by the bacterial MutS2 protein.Mol Cell17113120
- 39. McNulty SL, Mole BM, Dailidiene D, Segal I, Ally R, et al. (2004) Novel 180- and 480-base-pair insertions in African and African-American strains of Helicobacter pylori. J Clin Microbiol 42: 5658–5663.SL McNultyBM MoleD. DailidieneI. SegalR. Ally2004Novel 180- and 480-base-pair insertions in African and African-American strains of Helicobacter pylori.J Clin Microbiol4256585663
- 40. Torii N, Nozaki T, Masutani M, Nakagama H, Sugiyama T, et al. (2003) Spontaneous mutations in the Helicobacter pylori rpsL gene. Mutat Res 535: 141–145.N. ToriiT. NozakiM. MasutaniH. NakagamaT. Sugiyama2003Spontaneous mutations in the Helicobacter pylori rpsL gene.Mutat Res535141145
- 41. Wang G, Wilson TJ, Jiang Q, Taylor DE (2001) Spontaneous mutations that confer antibiotic resistance in Helicobacter pylori. Antimicrob Agents Chemother 45: 727–733.G. WangTJ WilsonQ. JiangDE Taylor2001Spontaneous mutations that confer antibiotic resistance in Helicobacter pylori.Antimicrob Agents Chemother45727733