Ebolavirus is responsible for highly lethal hemorrhagic fever. Like all viruses, it must reproduce its various components and assemble them in cells in order to reproduce infectious virions and perpetuate itself. To generate infectious Ebolavirus, a viral genome-protein complex called the nucleocapsid (NC) must be produced and transported to the cell surface, incorporated into virions, and then released from cells. To further our understanding of the Ebolavirus life cycle, we expressed the various viral proteins in mammalian cells and examined them ultrastructurally and biochemically. Expression of nucleoprotein alone led to the formation of helical tubes, which likely serve as a core for the NC. The matrix protein VP40 was found to be critical for transport of NCs to the cell surface and for the incorporation of NCs into virions, where interaction between nucleoprotein and the matrix protein VP40 is likely essential for these processes. Examination of virus-infected cells revealed that virions containing NCs mainly emerge horizontally from the cell surface, whereas empty virions mainly bud vertically, suggesting that horizontal budding is the major mode of Ebolavirus budding. These data form a foundation for the identification and development of potential antiviral agents to combat the devastating disease caused by this virus.
Ebolavirus is a causative agent of a severe, mostly fatal hemorrhagic fever in humans. Like all viruses, it must reproduce its various components and assemble them to reproduce progeny virus particles. However, because Ebolavirus needs special containment for biosafety, progress in understanding its morphogenesis has been slow, resulting in no effective therapies currently available for humans. To further our understanding of the Ebolavirus life cycle, the authors examined the specific interactions among viral proteins and the processes of Ebolavirus morphogenesis.
It is demonstrated that nucleoprotein likely serves as a core for the nucleocapsid (NC) complex, which is critical for replication and transcription of viral genome. The interaction of nucleoprotein with matrix protein VP40 is found to be essential for transport of NCs to the cell surface and for the incorporation of NCs into virions, resulting in the formation of mature virus particles. Unique among all viruses to our knowledge, Ebolavirus particles containing NCs mainly emerge horizontally from the cell surface, whereas the other viruses bud vertically. These findings form a foundation for the identification and development of potential antiviral agents to combat the devastating disease caused by this virus.
Citation: Noda T, Ebihara H, Muramoto Y, Fujii K, Takada A, Sagara H, et al. (2006) Assembly and Budding of Ebolavirus. PLoS Pathog 2(9): e99. https://doi.org/10.1371/journal.ppat.0020099
Editor: Skip Virgin, Washington University School of Medicine, United States of America
Received: March 3, 2006; Accepted: August 16, 2006; Published: September 29, 2006
Copyright: © 2006 Noda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by Core Research for Evolutional Science and Technology (CREST) grants from the Japan Science and Technology Corporation Agency (JST), Japan; by grants from the National Institutes of Health, NIAID; by grants-in-aid from the Ministry of Education, Culture, Sports, Science, and Technology and the Ministry of Health, Labor, and Welfare, Japan; and by the NIH/NIAID Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research (RCE) Program. The authors also acknowledge membership within and support from the Region V “Great Lakes” RCE (NIH award 1-U54-AI-057153). TN was the recipient of Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists.
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: EYFP, enhanced yellow fluorescent protein; GP, glycoprotein; NC, nucleocapsid; NP, nucleoprotein; SEM, scanning electron microscopy; TEM, transmission electron microscopy; VLP, virus-like particle; VP40, viral matrix protein
Ebolavirus, together with Marburgvirus, comprise the family Filoviridae in the order Mononegavirales [1,2]. It has a nonsegmented, negative-sense RNA genome that encodes at least seven structural proteins [1,3]. These proteins form filamentous particles 80 nm in diameter with lipid membrane derived from host cells. The viral glycoprotein (GP) protrudes from the surface of the viral envelope, while the matrix protein VP40 plays a central role in the morphogenesis of the filamentous virions [4–7]. Along the central axis of the filamentous virion resides a nucleocapsid (NC) of approximately 50 nm in diameter. This viral genomic RNA-protein complex has an axial channel at its center . The NCs of Ebolavirus, which represent the principal units of transcription and replication of the viral genome, are thought to consist of four proteins: the L polymerase protein, VP35, nucleoprotein (NP), and VP30. Huang et al.  showed that expression of NP, the membrane-associated VP24 protein, and VP35 results in the formation of structures morphologically indistinguishable from the NCs observed in Ebolavirus-infected cells, demonstrating the involvement of VP24 in the formation of such structures.
In Ebolavirus infection, newly synthesized viral proteins and genomic RNA in the form of NCs are transported to the budding site where the viral components assemble to form virions [7,10–13]. However, many questions regarding assembly of Ebolavirus particles have yet to be answered. How are NCs formed in the cytoplasm? How are NCs transported to the cell surface and incorporated into virions? How do the filamentous virions bud from the cell surface? In an attempt to answer these questions, we performed structural and biochemical assessments of cells transfected with plasmids expressing various combinations of Ebola viral proteins and of cells infected with Ebolavirus.
Formation of NC-Like Structures
To confirm whether the expression of NP, VP24, and VP35 are sufficient for the formation of NC-like structures [9,14], we cotransfected 293T cells with various combinations of plasmids for the expression of the viral proteins (i.e., NP, VP24, VP30, VP35, and L) and the minigenome RNA, which consists of a green fluorescent protein gene flanked by 3′ leader and 5′ trailer sequences. As previously described [9,14], coexpression of NP, VP24, and VP35 was indispensable for the formation of NC-like structures (Figures 1A and S1A) that are morphologically indistinguishable from NCs in virus-infected cells (Figure 1B), whereas the viral minigenome RNA was not essential for this process. To understand the roles of the individual proteins in the formation of the NC-like structures, we transfected cells with plasmids expressing NP, VP24, or VP35. When NP was expressed alone, helical tubes, the diameter of which was almost the same as that of a central portion of the NCs (approximately 20–25 nm in diameter), possessing a central channel (approximately 15–20 nm in diameter), were arranged in a bundle in the cytoplasm (Figure S1B–S1D). In the cytoplasm of VP35-expressing cells, large (approximately 8-μm × 3-μm) electron-dense structures with small opaque areas (less than 200 nm in diameter) were observed near the nucleus (Figure S1E). By contrast, in cells transfected with the VP24 plasmid, numerous, small electron-dense pleiomorphic aggregates, identified as VP24 by immunoelectron microscopy with an anti-VP24 antibody (unpublished data), were seen scattered throughout the cytoplasm (Figure S1F).
(A) Expression of NP, VP24, and VP35 produced filamentous and tubular NC-like structures approximately 50 nm in diameter.
(B) In Ebolavirus-infected cells, a large number of NCs newly synthesized in the cytoplasm were observed.
(C) When NP, VP24, and VP35 were expressed simultaneously, NC-like structures were found at the edge of the NP tubes. Bars, 1 μm (A, B, and C).
To understand how NC-like structures are formed, we coexpressed NP, VP24, and VP35 in different combinations. On coexpression of VP35 with VP24, we saw a large number of pleiomorphic particles near the nucleus that were different from the VP35- or VP24-induced structures (Figure S1G), although an interaction between VP35 and VP24 was not detected by a coimmunoprecipitation assay (unpublished data). When VP35 was expressed with NP, small pleiomorphic structures, whose electron density was similar to that of the VP35-induced structures, were observed at the periphery of the clusters formed with NP tubes (Figure S1H). By contrast, when VP24 was expressed with NP, the morphologies of the small pleiomorphic structures formed by VP24 and of the NP tubes did not change (unpublished data). Finally, when the three proteins were expressed simultaneously, NC-like structures approximately 50 nm in diameter were found at the edge of the clusters of NP tubes (Figure 1C). These results, together with the previous biochemical studies demonstrating interactions between NP and VP24 (Ebolavirus) or NP and VP35 (Ebolavirus and Marburgvirus) [9,15], suggest that NP helical structures likely serve as the core for the formation of the NC-like structures, and that VP35 and VP24 contribute to this process by interacting with NP at the periphery of the NP clusters.
VP40 Is Critical for the Transport of NC-Like Structures and for Virion Incorporation
Upon expression of NP, VP35, and VP24, NC-like structures were not found at the plasma membrane where Ebolavirus buds off, indicating that the formation and transportation of NCs to the cell surface are separate events and likely independently regulated. To determine which viral proteins are required for NC transport to the cell surface, we expressed the viral proteins involved in the formation of NC-like structures, other viral proteins (i.e., L, VP30, VP40, and GP), and the minigenome RNA in 293T cells. When VP40 was coexpressed with the proteins required for the NC-like structures (i.e., NP, VP24, and VP35), these structures were found immediately beneath the plasma membrane (Figure 2A) in an orderly arrangement, even in the absence of other viral proteins (i.e., L, VP30, and GP) and the minigenome viral RNA (unpublished data). Furthermore, VP40 proteins were detected by immunoelectron microscopy near the NC-like structures underneath the plasma membrane (Figure S2A). However, without VP40, NC-like structures at the plasma membrane could not be detected (Figure S2B). These results indicate that VP40 plays an important role in the transport of NC-like structures to the cell surface.
(A) A micrograph of the transport of NC-like structures in cells expressing NP, VP24, VP35, and VP40. (A, inset) Enlargement of the boxed area depicting the cross section of six NC-like structures beneath the plasma membrane.
(B) NC-like structures were incorporated into VLPs. Inside the VLPs, tubular NC-like structures are observed. NC-like structures released from VLPs broken during sample preparation can also be seen in the same field (arrow).
(C and D) An NC-like structure (indicated by broken lines in D) residing along the central axis of a filamentous VLP. Bars, 2μm (A), 500 nm (B), or 100 nm (C).
Expression of VP40 results in the formation of virus-like particles (VLPs), which are released from plasmid-transfected cells [4,5]. To determine whether NC-like structures are incorporated into VLPs, we examined the VLPs released from cells expressing NP, VP24, VP35, and VP40 by negative-staining electron microscopy. Smooth-surfaced, filamentous VLPs were found in the supernatants (Figure 2B). In most of the VLPs, NC-like structures were present along the central axis (Figure 2C and 2D), as is seen in NCs detected in authentic Ebola virions . These observations indicate that VP40 alone is sufficient for NC incorporation into virions and that the surface membrane GP is not required for this event, unlike influenza virus glycoproteins .
VLP Budding Is Dependent on Microtubules
To determine which cellular components are involved in the transport of the NC-like structures to the plasma membrane, we expressed NP, VP24, VP35, and VP40 in cells treated with an intracellular vesicular trafficking inhibitor (monensin), an actin polymerization inhibitor (cytochalasin D), a microtubule polymerization inhibitor (nocodazole), or a microtubule depolymerization inhibitor (taxol). Even when actin polymerization was disturbed by 10 μg/ml cytochalasin D (the effect of the drug was confirmed by a change in enhanced yellow fluorescent protein [EYFP-β] actin distribution visualized by fluorescence microscopy; not shown), the amounts of VP40 and NP detected in the supernatants of the drug-treated cells did not differ from those of untreated cells (Figure 3A), despite a previous report suggesting the involvement of actin in virion formation . When vesicular transport was inhibited by 5 μM monensin (a condition under which the transport of the vesicular stomatitis virus glycoprotein was confirmed to be inhibited ; unpublished data), a significant effect on release of VP40 and NP was not observed (Figure 3A). By contrast, perturbation of the microtubule structures by either 10 μM nocodazole or 1 μM taxol (as indicated by changes in EYFP-tubulin distribution; not shown) led to a reduction in the levels of NP and VP40 detected in the culture supernatants by more than 56% for NP and more than 57% for VP40 (Figure 3A).
10 μM nocodazole (noc), 1 μM taxol (tax), 10 μg/ml cytochalasin D (cytD), or 5 μM monensin (mon) was added to cells 3 h after they were transfected with plasmids expressing (A) NP, VP24, VP35, and VP40, or (B) VP40 alone. At 16 h post-transfection, proteins in the cell lysates and supernatants were separated by SDS-PAGE and examined by Western blotting with anti-NP and anti-VP40 antibodies. Following nocodazol or taxol treatment, the amounts of both VP40 and NP (A) or VP40 (B) in the supernatants (i.e., efficiency of VLPs budding) were reduced. cont, mock-treated control.
Using electron microscopy we next examined whether microtubule-perturbating drugs inhibit the transport of NC-like structures to plasma membrane. In the presence of nocodazole or taxol, we did not detect an appreciable difference in the intracellular localization of NC-like structures, by comparison to localization in the absence of these drugs (unpublished data). We therefore tested the effect of these drugs on VLPs produced by VP40 alone. We found that perturbation of microtubules with these drugs reduced VP40-induced VLP release into culture media by 62% for nocodazole and 64% for taxol (Figure 3B). This finding is consistent with a recent report that Ebolavirus VP40 directly associates with microtubules . These data suggest that a microtubule-dependent pathway may be involved in the release of VLPs from cells, which likely explains the concomitant reduction of NC-like structures in the culture supernatant.
Interaction between NP and VP40 during the Formation of VP40-Induced VLPs
When VP40 and NP are coexpressed, NP migrates beneath the plasma membrane , and large amounts of NP are detectable in the supernatant . To make certain that the NP detected in the supernatant was indeed incorporated into VLPs and not released as free NP, we performed a floatation analysis with the supernatants of cells expressing either NP alone or NP and VP40. When NP was coexpressed with VP40 (Figure S3A), it was detected in fractions of low sucrose concentration (i.e., approximately 1.12 g/cm3, fraction number 3) and as free protein in fractions of higher sucrose concentration. However, when NP was expressed alone, it was not detected in the supernatant of the transfected cells (Figure S3B). These results suggest that NP exists in membrane-bound form in the supernatant of cells that express both NP and VP40.
To obtain direct morphologic evidence that NP is incorporated into VLPs, we used transmission electron microscopy (TEM) to examine cells coexpressing NP and VP40. Following coexpression of NP and VP40, we observed filamentous VLPs containing NP helical structures (Figure 4A), which were not observed in VLPs produced by the expression of VP40 alone (Figure 4C). Indeed, helical NP structures were found even in VLPs that were just about to bud (Figure 4B, arrows). To demonstrate a direct interaction between VP40 and NP, we transfected 293T cells with a VP40-expressing plasmid alone or together with an NP-expressing plasmid and subjected the cell lysates and supernatants to immunoprecipitation with appropriate antibodies. VP40 coimmunoprecipitated with NP, and conversely, NP with VP40, demonstrating the direct interaction between these two proteins (Figure S4A and S4B). These results strongly suggest that the interaction between NP and VP40 is responsible for the incorporation of NCs into virions.
(A and B) In cells coexpressing NP and VP40, NP helices can be seen in newly released VP40-induced VLPs (A, arrow), as well as in VLPs just about to bud (B, arrows).
(C) Empty VLPs released from cells expressing VP40 alone. Bars, 100 nm (A and C) or 500 nm (B).
Ebolavirus Buds in Two Distinct Modes
To gain insight into the budding process of Ebolavirus, we examined Vero E6 cells infected with Ebolavirus by scanning electron microscopy (SEM) 2 d post-infection. Unlike uninfected Vero E6 cells (Figure S5A), most of the Ebolavirus-infected cells “rounded up” (Figure S5B), most likely because of GP-mediated downregulation of cellular adhesion molecules, including integrins [22,23]. In these virus-infected cells, which harbored many virions on their surface, two different modes of virus budding were observed. Filamentous virions were released vertically from the cell surface (Figure 5A), as previously documented for other filamentous virions [24–26], but, in addition, many virions emerged horizontally through the plasma membrane (Figure 5B). In our studies, both types of budding were rarely found in the same cells. Since horizontally budding viruses have never been reported previously, we further studied these horizontally budding virions by both SEM and TEM. At an early stage of horizontal budding, an orderly array of filaments began to emerge (Figure 5C, 5F, and 5I). As the horizontal budding progressed further (Figure 5D, 5G, and 5J), the membranes of the pre-virions were still connected to the cell. The filamentous Ebola virions eventually broke off, completing the budding process (Figure 5E, 5H, and 5K). These results show that filamentous Ebola virions do indeed bud from the plasma membrane horizontally.
(A) Filamentous virions, approximately 80 nm in diameter, budding from the cell surface vertically or (B) horizontally. (C, F, and I, early stage; D, G and J, intermediate stage; E, H, and K, final stage).
(C–E) The various steps of horizontal budding observed by SEM. Longitudinal (F–H) and transverse (I–K) sections of virions at different stages of horizontal budding visualized by TEM. Bars, 3 μm (A and B), 200 nm (C–H), or 100 nm (I–K).
Mature Ebola Virions Containing NCs Primarily Bud Horizontally
To establish the prevalence of these two modes of Ebolavirus budding, we randomly selected virus-infected cells, 2 d post-infection, that had rounded up and were producing virions (n = 123) and used SEM to determine what percentage were covered with horizontally budding virions. More than 80% of the cells were producing virions horizontally, indicating that horizontal budding is the dominant mode of Ebolavirus budding. What determines the mode of Ebolavirus budding? One possibility is the presence of NCs and their interaction with the matrix protein VP40. Support for this hypothesis comes from our previous observation that expression of VP40 produces VLPs that lack NCs , and that these VLPs bud vertically (Figure 6C). For this reason, we examined the NCs inside particles budding horizontally or vertically. Most of the horizontally budding virions contained NCs. Empty particles were rarely found (Figure 6A). By contrast, although some of the vertically budding virions contained NCs, most were empty particles and had slightly smaller diameters than the horizontally budding virions that contained NCs, likely because of the lack of NCs (Figure 6B). We counted more than 100 virus particles containing NC (n = 109) in ultrathin sections to determine the ratio of horizontally budding virions to vertically budding virions. 98.2% of the virus particles possessing NC inside the particles emerged horizontally from the cell surface. Therefore, we conclude that Ebola virions possessing NCs mainly bud horizontally.
(A) The majority of the virions is emerging horizontally from the cell surface and contains nucleocapsids (B), whereas vertically budding virions appear empty. Insets in (A) and (B) are larger magnifications of the areas identified in boxes of the main pictures, depicting cross sections of VLPs. (C) VLPs induced by the expression of VP40 alone bud vertically and lack NC-like structures. Bars, 400 nm.
Since the matrix proteins of many negative-strand RNA viruses are known to interact with their NCs during assembly , it is conceivable that the NCs and the matrix proteins of Ebolavirus affect the budding mode of virions. Because Ebolavirus NCs synthesized in the cytoplasm are conveyed to the plasma membrane by a mechanism involving VP40 (Figure 2A), it may be that the empty VLPs induced by VP40 are released vertically because they did not accumulate enough NCs in the cytoplasm.
Our findings, together with previous biochemical data [9,15], suggest a model for Ebola virion formation (see Figure 7): NP self-assembles to helical tubes. VP35 and VP24 possibly interact with NP around the mass of NP tubes, resulting in the formation of NC-like structures. The NC-like structures are then transported to the cell surface via a microtubules-dependent pathway mediated by VP40. Finally, the NCs are incorporated into virions through an interaction between NP and VP40 and bud mainly horizontally from the cell surface. Thus, our study provides morphologic evidence of interactions among viral proteins during the processes of virion assembly and budding. These results clearly further our understanding of the Ebolavirus life cycle. More detailed investigation should provide attractive targets for the development of antiviral compounds, such as inhibitors of virion assembly, formation, or budding.
(A) NP self-assembles to form helical structures.
(B) VP35 and VP24 interact with the helical structures formed by NP, resulting in the formation of NC-like structures (C).
(D) NC-like structures are transported to the plasma membrane in the presence of VP40 via a microtubule-dependent pathway.
(E) NC-like structures are incorporated into VLPs through an interaction between VP40 and NP that results in the production of filamentous particles budding horizontally from the cell surface.
Materials and Methods
Cells and virus.
293T cells and Vero E6 cells were maintained as described previously . Vero E6 cells were infected with Zaire ebolavirus at a multiplicity of infection of 0.5. All infectious materials were handled in the biosafety level 4 facility of the National Microbiology Laboratory, Public Health Agency of Canada, at the Canadian Science Center for Human and Animal Health, Winnipeg, Manitoba, Canada.
Plasmids and cell transfection.
All Zaire ebolavirus cDNAs were cloned separately into the mammalian expression vector pCAGGS/MCS [29,30]. A plasmid expressing an Ebolavirus minigenome, which contains a green fluorescent protein (GFP) gene flanked by 3′ leader and 5′ trailer sequences was described elsewhere [28,31]. 293T cells grown in 6-well plates were transfected with plasmids using Trans IT 293 reagent (Mirus Corporation, Madison, Wisconsin, United States).
Cytochalasin D, nocodazole, taxol, and monensin sodium salt were purchased from Sigma-Aldrich (St. Louis, Missouri, United States). Final concentrations of 10 μM Nocodazole (noc), 1 μM taxol (tax), 10 μg/ml cytochalasin D (cytD), or 5 μM monensin (mon) were added to culture media 3 h post-transfection of plasmids, and samples were harvested 16 h post-transfection.
293T cells transfected with protein expression plasmids were lysed in an SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) sample buffer, and separated on a 4%–20% PAGE Tris-glycine gradient gel (Daiichi Pure Chemicals Company, Tokyo, Japan). Blots were then incubated with rabbit anti-NP and anti-VP40 serum, and binding of the primary antibodies was detected with the Vectastain ABC kit (Vector Laboratories, Incorporated, Burlingame, California, United States). Bands were then visualized by immunostaining (HRP-1000, Konica Minolta Holdings, Incorporated, Tokyo, Japan), and their relative intensities calculated by using Scion Image (http://www.scioncorp.com).
TEM and SEM.
TEM and immuno-TEM were carried out as described previously . More than 50 transfected cells were examined for each test sample. For negative staining, the culture supernatant of 293T cells was stained with 5% uranyl acetate. All samples described above were examined with a JEM-1200EX electron microscope at 80 kV. SEM was performed as described previously .
To determine the effects of drugs (nocodazole, taxol, and cytochalasin D) on NC-like structure transport and incorporation into virions, 293T cells were transfected with 0.5 μg of pEYFP-actin expressing enhanced yellow fluorescence protein, (EYFP)-β actin fusion protein, or pEYFP-tublin expressing EYFP-α actin fusion protein (Becton Dickinson, Franklin Lakes, New Jersey, United States) and then treated with the drugs 3 h post-transfection. At 16 h post-transfection, the EYFP proteins were visualized with a BZ-8000 fluorescence microscope (Keyence, Osaka, Japan).
Supernatants of plasmid-transfected 293T cells grown in 6-well plates were concentrated to 500 μl with Amicon Ultra-4 (Millipore, Billerica, Massachusetts, United States) and mixed with 1.5 ml of 80% sucrose. They were then overlaid with 2 ml of 50% sucrose and 1.2 ml of 10% sucrose in SW55 centrifuge tubes, subjected to centrifugation in a Beckman SW55 rotor at 250,000 × g at 4 °C for 16 h, and fractionated (0.6 ml/fraction) from the top.
293T cells were transfected with a plasmid expressing VP40 alone or together with an NP-expressing plasmid, and 48 h later lysed in TNE buffer (10 mM Tris-HCl [pH 7.8], 1% NP40, 0.15M NaCl, 1 mM EDTA). Supernatants of similarly transfected cells were also harvested 48 h post-transfection, concentrated by using Amicon Ultra-4 (Millipore), and then lysed in the same buffer. After clarification by centrifugation, the supernatants were incubated with a rabbit anti-NP antibody (1:500 dilution). Immune complexes were precipitated by incubation with protein G sepharose beads (New England Biolabs, Incorporated, Beverly, Massachusetts, United States), which were suspended in the sample buffer. After removal of the sepharose beads, the samples were subjected to SDS-PAGE, followed by Western blot analysis with a rabbit anti-VP40 antibody. For reciprocal immunoprecipitation assays, cells were transfected with a plasmid for expression of NP alone or together with a plasmid for expression of FLAG-tagged VP40, and the assays were performed with cell lysates and the VLPs in the culture supernatants.
Figure S1. Electron Micrographs of Cells Expressing NP, VP24, and VP35
(A) Coexpression of NP, VP24, and VP35 resulted in the formation of NC-like structures.
(B–D) Expression of NP alone produced a mass of helical tubes (C, dotted line) approximately 20–25 nm in diameter near the nucleus (Nuc) (B). Transverse sections of the NP helices are seen on the right (D), while longitudinal sections can be seen on the left (D).
(E) Expression of VP35 alone formed large electron-dense aggregates (arrow) near the nucleus (nuc).
(F) Expression of VP24 alone resulted in numerous small pleiomorphic structures (arrows) near the nucleus (nuc).
(G) Coexpression of VP35 and VP24 produced large structures near nucleus (nuc) that differed from the aggregates formed by expression of either VP24 or VP35 alone.
(H) Accumulation of electron-dense aggregates around the mass of NP tubes (arrows) was observed in the cytoplasm of cells coexpressing NP and VP35. Bars, 50 nm (A and B), 2 μm (C, E, G, and H), 500 nm (D), or 1 μm (F).
(713 KB PDF)
Figure S2. VP40 Is Essential for the Transport of NC-Like Structures to the Cell Surface
(A) In cells expressing NP, VP24, VP35, and VP40, VP40 was detected by an anti-VP40 antibody conjugated with 15 nm gold, near to the NC-like structures (white arrows) located beneath the plasma membrane.
(B) Upon expression of all viral proteins except VP40 and the minigenome viral RNA, NC-like structures (arrows) remained in the cytoplasm. Bars, 100 nm (A) or 1 μm (B).
(294 KB PDF)
Figure S3. Floatation Analysis of the Supernatants of Cells Expressing NP Alone or with VP40
(A) Coexpression of NP and VP40 led to the detection of both proteins in the same fractions of the lower sucrose concentrations (fraction number 3).
(B) By contrast, when NP was expressed alone, it was not detected in the supernatant.
(69 KB PDF)
Figure S4. Immunoprecipitation Analysis of the Interaction between VP40 and NP
(A) VP40 was expressed alone (VP40) or together with NP (VP40 + NP). Cell lysates and supernatants were immunoprecipitated with an anti-NP antibody and then subjected to Western blotting with an anti-VP40 antibody.
(B) NP was expressed alone (NP) or together with FLAG-tagged VP40 (VP40F). Cell lysates and supernatants were immunoprecipitated with an anti-FLAG antibody and then subjected to Western blotting with an anti-NP antibody.
(685 KB PDF)
Figure S5. Morphologic Changes of Vero E6 Cells upon Ebolavirus Infection
Vero E6 cells, grown on cover slips, were infected with Ebolavirus and observed by SEM. (A) Control Vero E6 cells and (B) virus-infected cells 48 h post-infection. Cell rounding occurs only in virus-infected cells. (B, inset) Enlarged portion of the picture shown by square in (B). The cell surface is covered with numerous horizontally budding virions. Bars, 10 μm.
(1.8 MB PDF)
We thank Elke Mühlberger and Hans-Dieter Klenk for providing us with Ebola viral genes and Daryl Dick for operating the BSL4 in the Public Health Agency of Canada. We also thank Susan Watson for editing the manuscript.
HK and YK conceived and designed the experiments. TN, HE, YM, KF, AT, HS, and JHK performed the experiments. TN and YK wrote the paper.
- 1. Feldmann H, Jones S, Klenk HD, Schnittler HJ (2003) Ebola virus: From discovery to vaccine. Nat Rev Immunol 3: 677–685.H. FeldmannS. JonesHD KlenkHJ Schnittler2003Ebola virus: From discovery to vaccine.Nat Rev Immunol3677685
- 2. Sanchez A, Khan AS, Zaki SR, Nabel GJ, Ksiazek TG, et al. (2001) Filoviridae: Marburg and Ebola viruses. In: Knipe DM, Howley PM, editors. Fields virology. 4th edition. Philadelphia: Lippincott/The Williams and Wilkins Company. pp. 1279–1304.A. SanchezAS KhanSR ZakiGJ NabelTG Ksiazek2001Filoviridae: Marburg and Ebola viruses.In:. DM KnipePM HowleyFields virology. 4th editionPhiladelphiaLippincott/The Williams and Wilkins Company12791304
- 3. Feldmann H, Geisbert TW, Jahrling PB, Klenk H, Netesov SV, et al. (2004) Filoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA, editors. Virus taxonomy, VIIIth report of the ICTV. London: Elsevier Academic Press. pp. 645–653.H. FeldmannTW GeisbertPB JahrlingH. KlenkSV Netesov2004Filoviridae.In:. CM FauquetMA MayoJ. ManiloffU. DesselbergerLA BallVirus taxonomy, VIIIth report of the ICTVLondonElsevier Academic Press645653
- 4. Timmins J, Scianimanico S, Schoehn G, Weissenhorn W (2001) Vesicular release of Ebola virus matrix protein VP40. Virology 283: 1–6.J. TimminsS. ScianimanicoG. SchoehnW. Weissenhorn2001Vesicular release of Ebola virus matrix protein VP40.Virology28316
- 5. Noda T, Sagara H, Suzuki E, Takada A, Kida H, et al. (2002) Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP. J Virol 76: 4855–4865.T. NodaH. SagaraE. SuzukiA. TakadaH. Kida2002Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP.J Virol7648554865
- 6. Jasenosky LD, Neumann G, Lukashevich I, Kawaoka Y (2001) Ebola virus VP40-induced particle formation and association with the lipid bilayer. J Virol 75: 5205–5214.LD JasenoskyG. NeumannI. LukashevichY. Kawaoka2001Ebola virus VP40-induced particle formation and association with the lipid bilayer.J Virol7552055214
- 7. Harty RN, Brown ME, Wang G, Huibregtse J, Hayes FP (2000) A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: Implications for filovirus budding. Proc Natl Acad Sci U S A 97: 13871–13876.RN HartyME BrownG. WangJ. HuibregtseFP Hayes2000A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: Implications for filovirus budding.Proc Natl Acad Sci U S A971387113876
- 8. Geisbert TW, Jahrling PB (1995) Differentiation of filoviruses by electron microscopy. Virus Res 39: 129–150.TW GeisbertPB Jahrling1995Differentiation of filoviruses by electron microscopy.Virus Res39129150
- 9. Huang Y, Xu L, Sun Y, Nabel GJ (2002) The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein. Mol Cell 10: 307–316.Y. HuangL. XuY. SunGJ Nabel2002The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein.Mol Cell10307316
- 10. Bavari S, Bosio CM, Wiegand E, Ruthel G, Will AB, et al. (2002) Lipid raft microdomains: A gateway for compartmentalized trafficking of Ebola and Marburg viruses. J Exp Med 195: 593–602.S. BavariCM BosioE. WiegandG. RuthelAB Will2002Lipid raft microdomains: A gateway for compartmentalized trafficking of Ebola and Marburg viruses.J Exp Med195593602
- 11. Licata JM, Simpson-Holley M, Wright NT, Han Z, Paragas J, et al. (2003) Overlapping motifs (PTAP and PPEY) within the Ebola virus VP40 protein function independently as late budding domains: Involvement of host proteins TSG101 and VPS-4. J Virol 77: 1812–1819.JM LicataM. Simpson-HolleyNT WrightZ. HanJ. Paragas2003Overlapping motifs (PTAP and PPEY) within the Ebola virus VP40 protein function independently as late budding domains: Involvement of host proteins TSG101 and VPS-4.J Virol7718121819
- 12. Martin-Serrano J, Zang T, Bieniasz PD (2001) HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress. Nat Med 12: 1313–1319.J. Martin-SerranoT. ZangPD Bieniasz2001HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress.Nat Med1213131319
- 13. Timmins J, Schoehn G, Ricard-Blum S, Scianimanico S, Vernet T, et al. (2003) Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4. J Mol Biol 326: 493–502.J. TimminsG. SchoehnS. Ricard-BlumS. ScianimanicoT. Vernet2003Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4.J Mol Biol326493502
- 14. Noda T, Aoyama K, Sagara H, Kida H, Kawaoka Y (2005) Nucleocapsid-like structures of Ebola virus reconstructed using electron tomography. J Vet Med Sci 67: 325–328.T. NodaK. AoyamaH. SagaraH. KidaY. Kawaoka2005Nucleocapsid-like structures of Ebola virus reconstructed using electron tomography.J Vet Med Sci67325328
- 15. Becker S, Rinne C, Hofsäβ U, Klenk HD, Mühlberger E (1998) Interactions of Marburg virus nucleocapsid proteins. Virology 249: 406–417.S. BeckerC. RinneU. HofsäβHD KlenkE. Mühlberger1998Interactions of Marburg virus nucleocapsid proteins.Virology249406417
- 16. Zhang J, Leser GP, Pekosz A, Lamb RA (2000) The cytoplasmic tails of the influenza virus spike glycoproteins are required for normal genome packaging. Virology 269: 325–334.J. ZhangGP LeserA. PekoszRA Lamb2000The cytoplasmic tails of the influenza virus spike glycoproteins are required for normal genome packaging.Virology269325334
- 17. Han Z, Harty RN (2005) Packaging of actin into Ebola virus VLPs. Virol J 2: 92.Z. HanRN Harty2005Packaging of actin into Ebola virus VLPs.Virol J292
- 18. Alonso FV, Compans RW (1981) Differential effect of monensin on enveloped viruses that form at distinct plasma membrane domains. J Cell Biol 89: 700–705.FV AlonsoRW Compans1981Differential effect of monensin on enveloped viruses that form at distinct plasma membrane domains.J Cell Biol89700705
- 19. Ruthel G, Demmin GL, Kallstrom G, Javid M, Badie SS, et al. (2005) Association of Ebola virus matrix protein VP40 with microtubules. J Virol 79: 4709–4719.G. RuthelGL DemminG. KallstromM. JavidSS Badie2005Association of Ebola virus matrix protein VP40 with microtubules.J Virol7947094719
- 20. Kallstrom G, Warfield KL, Swenson DL, Mort S, Panchal RG, et al. (2005) Analysis of Ebola virus and VLP release using an immunocapture assay. J Virol Methods 127: 1–9.G. KallstromKL WarfieldDL SwensonS. MortRG Panchal2005Analysis of Ebola virus and VLP release using an immunocapture assay.J Virol Methods12719
- 21. Licata JM, Johnson RF, Han Z, Harty RN (2004) Contribution of Ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles. J Virol 78: 7344–7351.JM LicataRF JohnsonZ. HanRN Harty2004Contribution of Ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles.J Virol7873447351
- 22. Simmons G, Wool-Lewis RJ, Baribaud F, Netter RC, Bates P (2002) Ebola virus glycoproteins induce global surface protein down-modulation and loss of cell adherence. J Virol 76: 2518–2528.G. SimmonsRJ Wool-LewisF. BaribaudRC NetterP. Bates2002Ebola virus glycoproteins induce global surface protein down-modulation and loss of cell adherence.J Virol7625182528
- 23. Takada A, Watanabe S, Ito H, Okazaki K, Kida H, et al. (2000) Downregulation of beta1 integrins by Ebola virus glycoprotein: Implication for virus entry. Virology 278: 20–26.A. TakadaS. WatanabeH. ItoK. OkazakiH. Kida2000Downregulation of beta1 integrins by Ebola virus glycoprotein: Implication for virus entry.Virology2782026
- 24. Roberts PC, Compans RW (1998) Host cell dependence of viral morphology. Proc Natl Acad Sci U S A 95: 5746–5751.PC RobertsRW Compans1998Host cell dependence of viral morphology.Proc Natl Acad Sci U S A9557465751
- 25. Simpson-Holley M, Ellis D, Fisher D, Elton D, McCauley J, et al. (2002) A functional link between the actin cytoskeleton and lipid rafts during budding of filamentous influenza virions. Virology 301: 212–225.M. Simpson-HolleyD. EllisD. FisherD. EltonJ. McCauley2002A functional link between the actin cytoskeleton and lipid rafts during budding of filamentous influenza virions.Virology301212225
- 26. Brown G, Aitken J, Rixon HW, Sugrue RJ (2002) Caveolin-1 is incorporated into mature respiratory syncytial virus particles during virus assembly on the surface of virus-infected cells. J Gen Virol 83: 611–621.G. BrownJ. AitkenHW RixonRJ Sugrue2002Caveolin-1 is incorporated into mature respiratory syncytial virus particles during virus assembly on the surface of virus-infected cells.J Gen Virol83611621
- 27. Schmitt AP, Lamb RA (2004) Escaping from the cell: Assembly and budding of negative-strand RNA viruses. Curr Top Microbiol Immunol 283: 145–196.AP SchmittRA Lamb2004Escaping from the cell: Assembly and budding of negative-strand RNA viruses.Curr Top Microbiol Immunol283145196
- 28. Watanabe S, Watanabe T, Noda T, Takada A, Feldmann H, et al. (2004) Production of novel Ebola virus-like particles from cDNAs: An alternative to Ebola virus generation by reverse genetics. J Virol 78: 999–1005.S. WatanabeT. WatanabeT. NodaA. TakadaH. Feldmann2004Production of novel Ebola virus-like particles from cDNAs: An alternative to Ebola virus generation by reverse genetics.J Virol789991005
- 29. Niwa H, Yamamura K, Miyazaki J (1991) Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108: 193–199.H. NiwaK. YamamuraJ. Miyazaki1991Efficient selection for high-expression transfectants with a novel eukaryotic vector.Gene108193199
- 30. Kobasa D, Rodgers ME, Wells K, Kawaoka Y (1997) Neuraminidase hemadsorption activity, conserved in avian influenza A viruses, does not influence viral replication in ducks. J Virol 71: 6706–6713.D. KobasaME RodgersK. WellsY. Kawaoka1997Neuraminidase hemadsorption activity, conserved in avian influenza A viruses, does not influence viral replication in ducks.J Virol7167066713
- 31. Muhlberger E, Weik M, Volchkov VE, Klenk HD, Becker S (1999) Comparison of the transcription and replication strategies of Marburg virus and Ebola virus by using artificial replication systems. J Virol 73: 2333–2342.E. MuhlbergerM. WeikVE VolchkovHD KlenkS. Becker1999Comparison of the transcription and replication strategies of Marburg virus and Ebola virus by using artificial replication systems.J Virol7323332342