ࡱ > U@ > bjbj f 3 J J J J J J J $ n 6+ 6+ 6+ 6+ + d n D 2- p - - - - - - - D D D D D D D $ E R G H AD E J . - - . . AD J J - - D 5 5 5 . J - J - ? 5 . D 5 5 5 J J 5 - &- p53 6+ 3 5 9 D 0 D 5 H 4 H 5 n n J J J J H J 5 - " - 5 - - - - - AD AD n n D 5 n n Suppl. table S1
Strain or plasmidRelevant characteristicsSourcePlasmidspTnMax5
lacIq, tnpR, tnpA, res, orifd, catGC, CmR ADDIN EN.CITE Kahrs1995342124617Kahrs, A. F.Odenbreit, S.Schmitt, W.Heuermann, D.Meyer, T. F.Haas, R.Max-Planck-Institut fur Biologie, Abteilung Infektionsbiologie, Tubingen, Germany.An improved TnMax mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusionsGeneGene53-71671-2Base SequenceConjugation, Genetic*DNA Transposable ElementsGenes, Bacterial*Genetic VectorsMolecular Sequence Data*Mutagenesis*Recombinant Fusion Proteins1995Dec 290378-1119 (Print)8566811http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8566811 eng[1]pZERO-2Cloning vector, MCS in lacZ', neo, KmrInvitrogenpZERO-1Cloning vector, MCS in lacZ', zeo, ZeorInvitrogenp0060PCATpZERO-2, ( HP0058::Pcat, Cmr, KmrThis studyp0059PCATpZERO-2, ( HP0059::Pcat, Cmr, KmrThis studyp1143-PCATpZERO-2, ( HP1143::Pcat, Cmr, KmrThis studyp1143-PNEOpZERO-1, ( HP1143::Pneot, Zeor, KmrThis studypmreB-PCATpZERO-2, ( mreB::Pcat, Cmr, KmrThis studypASK-IBA7Expression vector, tetR, Ptet, bla, AprIBA, pIBA7-0058pASK-IBA7 carrying the HP0058 coding sequence under the control of the tet promoter cloned in the BsaI siteThis studypIBA7-0059pASK-IBA7 carrying the HP0059 coding sequence under the control of the tet promoter cloned in the BsaI siteThis studypIBA7-1142pASK-IBA7 carrying the HP1142 coding sequence under the control of the tet promoter cloned in the BsaI siteThis studypETDuet-1bla NovagenpETDuet-1143pETDuet-1 carrying the HP1143 coding sequence under the control of the T7promoter cloned between NcoI and BamHI siteThis studypSG1164bla, cat, Pxyl-gfpmut1 ADDIN EN.CITE Lewis1999212432124317Lewis, P. J.Marston, A. L.Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK. plewis@molbiol.ox.ac.ukGFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilisGeneGene101-102271*Artificial Gene FusionBacillus subtilis/*geneticsBase SequenceGene Expression Regulation*Genetic VectorsGreen Fluorescent ProteinsLuminescent Proteins/*geneticsMolecular Sequence DataPromoter Regions (Genetics)Transcription, Genetic1999Feb 40378-1119 (Print)9931458http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9931458 en g <