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Johanna E. Fraser, Jyotika Taneja De Bruyne, Iñaki Iturbe-Ormaetxe, Justin Stepnell, Rhiannon L. Burns, Heather A. Flores, Scott L. O’Neill
Novel Wolbachia-transinfected Aedes aegypti mosquitoes possess diverse fitness and vector competence phenotypes
www.plospathogens.org
www.plospathogens.org
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1.4683 0 Td
(goal)Tj
1.8765 0 Td
(of)Tj
1.0148 0 Td
(this)Tj
1.6725 0 Td
(work)Tj
2.279 0 Td
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1.6951 0 Td
(to)Tj
1.0262 0 Td
(determine)Tj
4.3256 0 Td
(if)Tj
0.7767 0 Td
(any)Tj
1.6554 0 Td
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(the)Tj
1.4683 0 Td
(newly)Tj
2.5796 0 Td
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-32.876 -1.2983 Td
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5.4311 0 Td
(lines)Tj
2.0693 0 Td
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3.6566 0 Td
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1.9673 0 Td
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(a)Tj
0.652 0 Td
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2.6986 0 Td
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-34.9963 -1.3039 Td
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3.4185 0 Td
(testing.)Tj
-2.2223 -1.2983 Td
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1.627 0 Td
(a)Tj
/F10 1 Tf
0.6577 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
(-transinfected)Tj
/F10 1 Tf
10.0232 0 Td
[()30()]TJ
/F9 1 Tf
(.)Tj
/F10 1 Tf
1.4853 0 Td
[()6()9()-21()-13()12( )7(\n)]TJ
/F9 1 Tf
2.9877 0 Td
(line)Tj
1.7065 0 Td
(to)Tj
1.0261 0 Td
(be)Tj
1.1509 0 Td
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4.5978 0 Td
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1.3889 0 Td
(examination)Tj
5.2327 0 Td
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-34.1175 -1.2983 Td
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5.5218 0 Td
(experiments,)Tj
5.3631 0 Td
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1.4683 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
4.4617 0 Td
(strain)Tj
2.5002 0 Td
(must)Tj
2.228 0 Td
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3.2882 0 Td
(strong)Tj
2.7722 0 Td
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4.3824 0 Td
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3.0274 0 Td
(virus)Tj
-35.0133 -1.3039 Td
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4.7451 0 Td
(demonstrate)Tj
5.2384 0 Td
(near-complete)Tj
6.0037 0 Td
(MT,)Tj
1.9503 0 Td
(and)Tj
1.7291 0 Td
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2.9367 0 Td
(CI)Tj
1.2245 0 Td
(to)Tj
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2.8006 0 Td
(rapid)Tj
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1.8425 0 Td
(the)Tj
-34.6901 -1.2982 Td
(wild)Tj
1.9445 0 Td
(mosquito)Tj
4.0138 0 Td
(population,)Tj
4.8132 0 Td
(whilst)Tj
2.6079 0 Td
(inducing)Tj
3.7927 0 Td
(minimal)Tj
3.6227 0 Td
(fitness)Tj
2.7666 0 Td
(cost)Tj
1.8141 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(mosquito)Tj
4.0138 0 Td
(host.)Tj
-30.6876 -1.2983 Td
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1.3379 0 Td
(predict)Tj
3.0501 0 Td
(which)Tj
/F10 1 Tf
2.6532 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
4.456 0 Td
(strains)Tj
2.863 0 Td
(may)Tj
1.9275 0 Td
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3.2882 0 Td
(these)Tj
2.2564 0 Td
(traits)Tj
2.2506 0 Td
(we)Tj
1.3267 0 Td
(looked)Tj
2.9253 0 Td
(to)Tj
1.0261 0 Td
(past)Tj
1.8312 0 Td
(studies,)Tj
3.2088 0 Td
(most)Tj
-35.5972 -1.3039 Td
(of)Tj
1.0148 0 Td
(which)Tj
2.6475 0 Td
(have)Tj
2.0636 0 Td
(been)Tj
2.1203 0 Td
(performed)Tj
4.4674 0 Td
(using)Tj
2.3924 0 Td
(natively)Tj
/F10 1 Tf
3.3448 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
(-infected,)Tj
8.2375 0 Td
(artificially)Tj
4.2179 0 Td
(transinfected,)Tj
5.6579 0 Td
(or)Tj
-36.1641 -1.2983 Td
(introgressed)Tj
/F10 1 Tf
5.142 0 Td
[()-4()20()7()-3()11()10()18(\n)4(\r)4()]TJ
/F9 1 Tf
4.507 0 Td
(lines,)Tj
2.2961 0 Td
(with)Tj
1.9955 0 Td
(the)Tj
1.4684 0 Td
(RNA)Tj
2.262 0 Td
(viruses)Tj
2.9877 0 Td
(Drosophila)Tj
4.6941 0 Td
(C)Tj
0.8845 0 Td
(virus)Tj
2.1996 0 Td
(\(DCV\))Tj
2.9934 0 Td
(or)Tj
1.0998 0 Td
(Flock)Tj
2.4208 0 Td
(House)Tj
-34.9509 -1.2982 Td
(virus)Tj
2.1996 0 Td
(\(FHV\))Tj
2.8913 0 Td
(as)Tj
1.0148 0 Td
(models.)Tj
3.3279 0 Td
(From)Tj
2.4491 0 Td
(these)Tj
2.2507 0 Td
(studies,)Tj
3.2145 0 Td
(we)Tj
1.3266 0 Td
(identified)Tj
/F10 1 Tf
4.0592 0 Td
[()]TJ
/F9 1 Tf
(MelCS)Tj
3.5829 0 Td
(\(from)Tj
/F10 1 Tf
2.5455 0 Td
[()]TJ
/F9 1 Tf
(.)Tj
/F10 1 Tf
1.1395 0 Td
[()6()15(\r)4()16()6()2()2()9( )11()2()]TJ
/F9 1 Tf
(\))Tj
5.726 0 Td
(and)Tj
/F10 1 Tf
-35.7276 -1.2983 Td
[()]TJ
/F9 1 Tf
(Ri)Tj
1.7687 0 Td
(\(from)Tj
/F10 1 Tf
2.5455 0 Td
[()]TJ
/F9 1 Tf
(.)Tj
/F10 1 Tf
1.1396 0 Td
[()10(\n)12()18()4(\r)4()16()]TJ
/F9 1 Tf
(\))Tj
4.0421 0 Td
(as)Tj
1.0148 0 Td
(key)Tj
1.5988 0 Td
(candidates)Tj
4.4617 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(].)Tj
-15.375 -1.3039 Td
(Although)Tj
/F10 1 Tf
4.0081 0 Td
[()30()]TJ
/F9 1 Tf
(.)Tj
/F10 1 Tf
1.491 0 Td
[()6()9()-21()-13()12( )7(\n)]TJ
/F9 1 Tf
2.9821 0 Td
(mosquitoes)Tj
4.8018 0 Td
(do)Tj
1.2529 0 Td
(not)Tj
1.5761 0 Td
(naturally)Tj
3.7814 0 Td
(carry)Tj
/F10 1 Tf
2.2733 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
(,)Tj
4.6828 0 Td
(a)Tj
0.6577 0 Td
(number)Tj
3.4129 0 Td
(of)Tj
1.0148 0 Td
(other)Tj
2.3527 0 Td
(mos-)Tj
-35.4838 -1.2983 Td
(quito)Tj
2.3243 0 Td
(species)Tj
2.9991 0 Td
(have)Tj
2.0579 0 Td
(natural)Tj
3.0784 0 Td
(infections)Tj
4.1556 0 Td
(including)Tj
4.0422 0 Td
(the)Tj
1.4683 0 Td
(closely)Tj
2.88 0 Td
(related)Tj
2.948 0 Td
(species)Tj
/F10 1 Tf
2.9934 0 Td
[()30()7()4()8()]TJ
2.4888 0 Td
[()11(\r)14()4()11()11(\n)4()-4( )7()-4()]TJ
/F9 1 Tf
-31.436 -1.2983 Td
(\()Tj
/F10 1 Tf
[()]TJ
/F9 1 Tf
(AlbA)Tj
3.3448 0 Td
(and)Tj
/F10 1 Tf
1.7291 0 Td
[()]TJ
/F9 1 Tf
(AlbB\))Tj
3.2428 0 Td
(and)Tj
/F10 1 Tf
1.7292 0 Td
[()30()7()4()8()]TJ
2.4831 0 Td
[()6()12( )11()7()-3()1()-9(\n)10()12( )7()-4()]TJ
/F9 1 Tf
4.9379 0 Td
(\()Tj
/F10 1 Tf
[()]TJ
/F9 1 Tf
(Noto\))Tj
3.6113 0 Td
(as)Tj
1.0148 0 Td
(well)Tj
1.8199 0 Td
(as)Tj
1.0148 0 Td
(more)Tj
2.3414 0 Td
(distantly)Tj
3.6566 0 Td
(related)Tj
2.9424 0 Td
(species)Tj
-33.8681 -1.3039 Td
(such)Tj
2.0579 0 Td
(as)Tj
/F10 1 Tf
1.0148 0 Td
[()13()4(\r)4()1()]TJ
2.4661 0 Td
[()14()12(\n)12()6()14()4()1()9()-3()1(\n)4()22( )7()-4()]TJ
/F9 1 Tf
6.7748 0 Td
(\()Tj
/F10 1 Tf
[()]TJ
/F9 1 Tf
(Pip\).)Tj
/F10 1 Tf
3.1294 0 Td
[()]TJ
/F9 1 Tf
(AlbB)Tj
2.9026 0 Td
(has)Tj
1.5421 0 Td
(previously)Tj
4.3483 0 Td
(been)Tj
2.126 0 Td
(transinfected)Tj
5.4311 0 Td
(into)Tj
/F10 1 Tf
1.8425 0 Td
[()30()]TJ
/F9 1 Tf
(.)Tj
/F10 1 Tf
-33.6356 -1.2982 Td
[()6()9()-21()-13()12( )7(\n)]TJ
/F9 1 Tf
2.9876 0 Td
(and)Tj
1.7292 0 Td
(shown)Tj
2.8459 0 Td
(to)Tj
1.0262 0 Td
(provide)Tj
3.2881 0 Td
(strong)Tj
2.7723 0 Td
(protection)Tj
4.3823 0 Td
(against)Tj
3.0274 0 Td
(DENV)Tj
2.9537 0 Td
(while)Tj
2.3697 0 Td
(showing)Tj
3.583 0 Td
(more)Tj
2.3414 0 Td
(resilience)Tj
-33.3068 -1.2983 Td
(to)Tj
1.0262 0 Td
(heat)Tj
1.8991 0 Td
(stress)Tj
2.3981 0 Td
(but)Tj
1.5533 0 Td
(poorer)Tj
2.9197 0 Td
(larval)Tj
2.4038 0 Td
(competitive)Tj
4.9209 0 Td
(ability)Tj
2.6929 0 Td
(compared)Tj
4.2463 0 Td
(to)Tj
/F10 1 Tf
1.0261 0 Td
[()]TJ
/F9 1 Tf
(Mel)Tj
2.4434 0 Td
([)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(25)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(29)Tj
0 g
(],)Tj
3.2088 0 Td
(indicating)Tj
-32.4734 -1.3039 Td
(the)Tj
1.4683 0 Td
(difficulty)Tj
3.8097 0 Td
(in)Tj
1.0318 0 Td
(assessing)Tj
3.8041 0 Td
(strains)Tj
2.863 0 Td
(for)Tj
1.3833 0 Td
(performance)Tj
5.3517 0 Td
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2.2054 0 Td
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3.8381 0 Td
(crude)Tj
2.4944 0 Td
(experimental)Tj
5.4595 0 Td
(studies)Tj
-33.7093 -1.2983 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(need)Tj
2.143 0 Td
(for)Tj
1.3889 0 Td
(comparative)Tj
5.1704 0 Td
(studies)Tj
2.9877 0 Td
(to)Tj
1.0205 0 Td
(be)Tj
1.1508 0 Td
(undertaken)Tj
4.8189 0 Td
(under)Tj
2.6192 0 Td
(field)Tj
1.9729 0 Td
(release)Tj
2.9027 0 Td
(conditions.)Tj
/F10 1 Tf
4.7054 0 Td
[()]TJ
/F9 1 Tf
(Pip)Tj
-34.0778 -1.2983 Td
(has)Tj
1.542 0 Td
(been)Tj
2.1203 0 Td
(reported)Tj
3.6623 0 Td
(to)Tj
1.0262 0 Td
(provide)Tj
3.2881 0 Td
(protection)Tj
4.3824 0 Td
(against)Tj
3.0273 0 Td
(West)Tj
2.2677 0 Td
(Nile)Tj
1.8936 0 Td
(virus)Tj
2.1996 0 Td
(\(WNV\))Tj
3.2995 0 Td
(in)Tj
1.0319 0 Td
(its)Tj
1.1508 0 Td
(natural)Tj
3.0784 0 Td
(host,)Tj
-33.9701 -1.2982 Td
(identifying)Tj
4.575 0 Td
(this)Tj
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(strain)Tj
2.5001 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.6576 0 Td
(possible)Tj
3.4073 0 Td
(candidate)Tj
4.1045 0 Td
(for)Tj
1.389 0 Td
(transinfection)Tj
5.7996 0 Td
(in)Tj
/F10 1 Tf
1.0375 0 Td
[()30()]TJ
/F9 1 Tf
(.)Tj
/F10 1 Tf
1.491 0 Td
[()6()9()-21()-13()12( )7(\n)]TJ
/F9 1 Tf
2.9877 0 Td
([)Tj
0.83 0.64 0.02 0 k
(30)Tj
0 g
(].)Tj
-29.4404 -1.3039 Td
(Here)Tj
2.211 0 Td
(we)Tj
1.3266 0 Td
(report)Tj
2.7099 0 Td
(the)Tj
1.4683 0 Td
(generation)Tj
4.5071 0 Td
(of)Tj
1.0148 0 Td
(three)Tj
2.262 0 Td
(new)Tj
/F10 1 Tf
1.8765 0 Td
[()30()]TJ
/F9 1 Tf
(.)Tj
/F10 1 Tf
1.491 0 Td
[()6()9()-21()-13()12( )7(\n)]TJ
/F9 1 Tf
2.9877 0 Td
(lines,)Tj
2.2904 0 Td
(transinfected)Tj
5.4312 0 Td
(with)Tj
/F10 1 Tf
2.0012 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
-32.7739 -1.2983 Td
(strains)Tj
/F10 1 Tf
2.8572 0 Td
[()]TJ
/F9 1 Tf
(Ri,)Tj
/F10 1 Tf
2.0013 0 Td
[()]TJ
/F9 1 Tf
(MelCS)Tj
3.583 0 Td
(and)Tj
/F10 1 Tf
1.7291 0 Td
[()]TJ
/F9 1 Tf
(Pip,)Tj
2.4548 0 Td
(and)Tj
1.7291 0 Td
(describe)Tj
3.5263 0 Td
(an)Tj
1.2019 0 Td
(initial)Tj
2.5454 0 Td
(relative)Tj
3.1408 0 Td
(evaluation)Tj
4.3597 0 Td
(of)Tj
1.0148 0 Td
(these)Tj
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(strains,)Tj
-32.3997 -1.2983 Td
(examining)Tj
4.456 0 Td
(the)Tj
1.474 0 Td
(effects)Tj
2.7212 0 Td
(on)Tj
1.2756 0 Td
(mosquito)Tj
4.0138 0 Td
(fitness,)Tj
2.9934 0 Td
(MT,)Tj
1.9502 0 Td
(CI,)Tj
1.4514 0 Td
(tissue)Tj
2.4547 0 Td
(tropism)Tj
3.3619 0 Td
(and)Tj
1.7291 0 Td
(DENV)Tj
2.9594 0 Td
(blocking.)Tj
3.9061 0 Td
(The)Tj
-34.7468 -1.3039 Td
(findings)Tj
3.4922 0 Td
(reveal)Tj
/F10 1 Tf
2.5738 0 Td
[()]TJ
/F9 1 Tf
(MelCS)Tj
3.583 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.652 0 Td
(potential)Tj
3.7644 0 Td
(candidate)Tj
4.1045 0 Td
(for)Tj
1.3833 0 Td
(future)Tj
2.6589 0 Td
(examination)Tj
5.2327 0 Td
(under)Tj
2.6192 0 Td
(more)Tj
2.3414 0 Td
(rigorous)Tj
-33.4202 -1.2982 Td
(field-relevant)Tj
5.5218 0 Td
(testing)Tj
2.88 0 Td
(and)Tj
1.7348 0 Td
(also)Tj
1.7631 0 Td
(indicate)Tj
3.4129 0 Td
(that)Tj
/F10 1 Tf
1.7801 0 Td
[()]TJ
/F9 1 Tf
(Ri)Tj
1.7689 0 Td
(and)Tj
/F10 1 Tf
1.7291 0 Td
[()]TJ
/F9 1 Tf
(Pip)Tj
2.228 0 Td
(can)Tj
1.6271 0 Td
(be)Tj
1.1508 0 Td
(removed)Tj
3.7474 0 Td
(as)Tj
1.0148 0 Td
(candidates)Tj
4.4674 0 Td
(for)Tj
-34.8262 -1.2983 Td
(future)Tj
2.6531 0 Td
(testing.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 247.9748 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Results)Tj
/F0 1 Tf
0 -1.4976 Td
(Maternal)Tj
4.063 0 Td
(transmission)Tj
5.7685 0 Td
(and)Tj
1.8662 0 Td
(cytoplasmic)Tj
5.3858 0 Td
(incompatibility)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 212.9952 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(MT)Tj
1.7234 0 Td
(of)Tj
1.0148 0 Td
(a)Tj
/F10 1 Tf
0.652 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
4.4617 0 Td
(strain)Tj
2.5001 0 Td
(and)Tj
1.7291 0 Td
(its)Tj
1.1452 0 Td
(ability)Tj
2.6929 0 Td
(to)Tj
1.0262 0 Td
(induce)Tj
2.9423 0 Td
(CI)Tj
1.2189 0 Td
(are)Tj
1.4513 0 Td
(key)Tj
1.5931 0 Td
(features)Tj
3.3505 0 Td
(that)Tj
1.7745 0 Td
(must)Tj
2.228 0 Td
(be)Tj
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(conserved)Tj
-32.6549 -1.2982 Td
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2.4037 0 Td
(considering)Tj
4.9323 0 Td
(new)Tj
/F10 1 Tf
1.8708 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
(-infected)Tj
/F10 1 Tf
8.0107 0 Td
[()30()]TJ
/F9 1 Tf
(.)Tj
/F10 1 Tf
1.491 0 Td
[()6()9()-21()-13()12( )7(\n)]TJ
/F9 1 Tf
2.9877 0 Td
(lines)Tj
2.0636 0 Td
(for)Tj
1.389 0 Td
(field)Tj
1.9729 0 Td
(releases.)Tj
3.4866 0 Td
(CI)Tj
1.2245 0 Td
(occurs)Tj
2.8346 0 Td
(when)Tj
-34.6674 -1.3039 Td
(an)Tj
1.2018 0 Td
(uninfected)Tj
4.4958 0 Td
(female)Tj
2.8573 0 Td
(mated)Tj
2.7212 0 Td
(with)Tj
1.9956 0 Td
(a)Tj
/F10 1 Tf
0.6576 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
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4.8982 0 Td
(decreased)Tj
4.1329 0 Td
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1.389 0 Td
(crosses)Tj
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(between)Tj
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(infected)Tj
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(females)Tj
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(and)Tj
-34.4293 -1.2983 Td
(males,)Tj
2.7268 0 Td
(and)Tj
1.7292 0 Td
(infected)Tj
3.4242 0 Td
(females)Tj
3.2145 0 Td
(crossed)Tj
3.1974 0 Td
(with)Tj
1.9956 0 Td
(uninfected)Tj
4.4957 0 Td
(males)Tj
2.5058 0 Td
(\(p)Tj
/F16 1 Tf
0 Tc
(<)Tj
/F9 1 Tf
-0.004 Tc
(0.001,)Tj
4.201 0 Td
(p)Tj
/F16 1 Tf
0 Tc
(<)Tj
/F9 1 Tf
-0.004 Tc
(0.05,)Tj
3.3845 0 Td
(respectively\),)Tj
-30.8747 -1.2982 Td
(suggesting)Tj
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4.3993 0 Td
[()]TJ
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(Pip)Tj
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(reduces)Tj
3.2768 0 Td
(fecundity)Tj
3.9798 0 Td
(for)Tj
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(this)Tj
1.6724 0 Td
(line.)Tj
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(next)Tj
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(examined)Tj
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(the)Tj
1.4683 0 Td
(egg)Tj
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(hatch)Tj
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(rates)Tj
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(of)Tj
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(each)Tj
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(cross.)Tj
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1.4683 0 Td
(significant)Tj
4.3881 0 Td
(reduction)Tj
4.1102 0 Td
(in)Tj
1.0374 0 Td
(hatch)Tj
2.4321 0 Td
(rate)Tj
-35.4214 -1.2982 Td
(was)Tj
1.695 0 Td
(observed)Tj
3.7984 0 Td
(for)Tj
/F10 1 Tf
1.3833 0 Td
[()]TJ
/F9 1 Tf
(Ri)Tj
1.7745 0 Td
(crosses)Tj
3.0274 0 Td
(between)Tj
/F10 1 Tf
3.5319 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
(-infected)Tj
8.005 0 Td
(females)Tj
3.2202 0 Td
(and)Tj
1.7291 0 Td
(males,)Tj
2.7269 0 Td
(when)Tj
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(compared)Tj
-33.2955 -1.2983 Td
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1.0262 0 Td
(Tet-treated)Tj
4.677 0 Td
(male)Tj
2.143 0 Td
(and)Tj
1.7291 0 Td
(female)Tj
2.8573 0 Td
(crosses.)Tj
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3.2542 0 Td
[()]TJ
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(showed)Tj
3.2485 0 Td
(a)Tj
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(slight)Tj
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(although)Tj
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(significant)Tj
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(reduction)Tj
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(in)Tj
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(the)Tj
1.4739 0 Td
(hatch)Tj
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(rate)Tj
1.7461 0 Td
(between)Tj
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3.5263 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
(-infected)Tj
8.0106 0 Td
(females)Tj
3.2145 0 Td
(and)Tj
1.7291 0 Td
(males,)Tj
2.7326 0 Td
(compared)Tj
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(to)Tj
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(the)Tj
1.4684 0 Td
(Tet-treated)Tj
-32.6322 -1.304 Td
(control)Tj
3.118 0 Td
(cross)Tj
2.2451 0 Td
(\(79%)Tj
2.2677 0 Td
(and)Tj
1.7347 0 Td
(90%,)Tj
2.1487 0 Td
(respectively;)Tj
5.1137 0 Td
(p)Tj
/F16 1 Tf
0 Tc
(<)Tj
/F9 1 Tf
-0.004 Tc
(0.05,)Tj
3.3845 0 Td
(Kruskal-Wallis,)Tj
6.4289 0 Td
(Dunn's)Tj
3.1578 0 Td
(test\))Tj
1.9389 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1B)Tj
0 g
(\),)Tj
1.8425 0 Td
(simi-)Tj
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(lar)Tj
1.2699 0 Td
(to)Tj
1.0261 0 Td
(that)Tj
1.7801 0 Td
(reported)Tj
3.6567 0 Td
(for)Tj
/F10 1 Tf
1.389 0 Td
[()]TJ
/F9 1 Tf
(Mel)Tj
2.4434 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(26)Tj
0 g
(].)Tj
1.7405 0 Td
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2.6702 0 Td
(rate)Tj
1.7461 0 Td
(was)Tj
1.7008 0 Td
(more)Tj
2.3414 0 Td
(dramatically)Tj
5.176 0 Td
(impaired)Tj
3.8495 0 Td
(for)Tj
/F10 1 Tf
1.3833 0 Td
[()]TJ
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-33.9078 -1.2983 Td
(with)Tj
1.9955 0 Td
(rates)Tj
2.109 0 Td
(dropping)Tj
3.9401 0 Td
(from)Tj
2.2054 0 Td
(82%)Tj
1.9275 0 Td
(for)Tj
1.389 0 Td
(the)Tj
1.4683 0 Td
(Tet-treated)Tj
4.6828 0 Td
(control)Tj
3.1181 0 Td
(cross,)Tj
2.4718 0 Td
(to)Tj
1.0261 0 Td
(58%)Tj
1.9276 0 Td
(for)Tj
/F10 1 Tf
1.3833 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
(-infected)Tj
-29.6445 -1.3039 Td
(male)Tj
2.1429 0 Td
(and)Tj
1.7291 0 Td
(female)Tj
2.8573 0 Td
(crosses)Tj
3.0274 0 Td
(\(p)Tj
/F16 1 Tf
0 Tc
(<)Tj
/F9 1 Tf
-0.004 Tc
(0.0001\).)Tj
5.0173 0 Td
(For)Tj
/F10 1 Tf
1.6271 0 Td
[()]TJ
/F9 1 Tf
(MelCS)Tj
3.583 0 Td
(and)Tj
/F10 1 Tf
1.7291 0 Td
[()]TJ
/F9 1 Tf
(Pip,)Tj
2.4548 0 Td
(crosses)Tj
3.0274 0 Td
(between)Tj
3.5262 0 Td
(infected)Tj
3.4186 0 Td
(females)Tj
-34.1402 -1.2983 Td
(and)Tj
1.7291 0 Td
(uninfected)Tj
4.4957 0 Td
(males)Tj
2.5058 0 Td
(also)Tj
1.7688 0 Td
(resulted)Tj
3.3959 0 Td
(in)Tj
1.0375 0 Td
(significant)Tj
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(reductions)Tj
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(in)Tj
1.0318 0 Td
(hatch)Tj
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(rate)Tj
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(\(68)Tj
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(and)Tj
1.7291 0 Td
(66%,)Tj
2.1543 0 Td
(com-)Tj
-34.4066 -1.2982 Td
(pared)Tj
2.4944 0 Td
(to)Tj
1.0261 0 Td
(respective)Tj
4.1783 0 Td
(Tet)Tj
1.5534 0 Td
(control)Tj
3.1237 0 Td
(cross)Tj
2.245 0 Td
(hatch)Tj
2.4322 0 Td
(rates)Tj
2.1089 0 Td
(of)Tj
1.0148 0 Td
(90)Tj
1.1679 0 Td
(and)Tj
1.7291 0 Td
(82%,)Tj
2.1543 0 Td
(p)Tj
/F16 1 Tf
0 Tc
(<)Tj
/F9 1 Tf
-0.004 Tc
(0.01\),)Tj
3.7304 0 Td
(perhaps)Tj
3.3675 0 Td
(suggesting)Tj
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(there)Tj
2.262 0 Td
(is)Tj
0.8504 0 Td
(a)Tj
0.6519 0 Td
(small)Tj
2.3301 0 Td
(fitness)Tj
2.7666 0 Td
(cost)Tj
1.8085 0 Td
(for)Tj
1.3833 0 Td
(these)Tj
2.2563 0 Td
(strains)Tj
2.863 0 Td
(of)Tj
/F10 1 Tf
1.0148 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
4.456 0 Td
(in)Tj
1.0375 0 Td
(eggs,)Tj
2.16 0 Td
(although)Tj
3.7531 0 Td
(these)Tj
2.2563 0 Td
(reductions)Tj
-31.8498 -1.2982 Td
(were)Tj
2.1202 0 Td
(similar)Tj
2.9877 0 Td
(to)Tj
1.0262 0 Td
(those)Tj
2.3357 0 Td
(described)Tj
4.0535 0 Td
(previously)Tj
4.3483 0 Td
(for)Tj
/F10 1 Tf
1.389 0 Td
[()]TJ
/F9 1 Tf
(Mel)Tj
2.4435 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(].)Tj
-19.5079 -1.2983 Td
(To)Tj
1.3379 0 Td
(determine)Tj
4.3256 0 Td
(the)Tj
1.4684 0 Td
(effect)Tj
2.3641 0 Td
(of)Tj
/F10 1 Tf
1.0147 0 Td
[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F9 1 Tf
4.4618 0 Td
(on)Tj
1.2755 0 Td
(egg)Tj
1.5761 0 Td
(viability)Tj
3.4129 0 Td
(and)Tj
1.7291 0 Td
(storage,)Tj
3.3052 0 Td
(eggs)Tj
1.9389 0 Td
(were)Tj
2.1203 0 Td
(collected)Tj
3.7417 0 Td
(and)Tj
-35.2684 -1.3039 Td
(stored)Tj
2.7098 0 Td
(for)Tj
1.3833 0 Td
(112)Tj
2.1657 0 Td
(weeks)Tj
2.6079 0 Td
(before)Tj
2.7382 0 Td
(determining)Tj
5.1817 0 Td
(hatch)Tj
2.4321 0 Td
(rates.)Tj
2.3357 0 Td
(The)Tj
1.7802 0 Td
(benchmark)Tj
4.7735 0 Td
(transinfected)Tj
5.4312 0 Td
(line,)Tj
/F10 1 Tf
-33.5393 -1.2983 Td
[()]TJ
/F9 1 Tf
(Mel)Tj
2.4434 0 Td
(had)Tj
1.7121 0 Td
(a)Tj
0.652 0 Td
(slight,)Tj
2.6135 0 Td
(but)Tj
1.5534 0 Td
(significant)Tj
4.388 0 Td
(reduction)Tj
4.1102 0 Td
(in)Tj
1.0375 0 Td
(hatch)Tj
2.4321 0 Td
(rate)Tj
1.7461 0 Td
(following)Tj
3.9798 0 Td
(4,)Tj
0.9184 0 Td
(8,)Tj
0.9241 0 Td
(and)Tj
1.7291 0 Td
(10)Tj
1.1736 0 Td
(weeks)Tj
2.6078 0 Td
(storage)Tj
-34.0211 -1.2982 Td
(\(p)Tj
/F16 1 Tf
0 Tc
(<)Tj
/F9 1 Tf
-0.004 Tc
(0.05,)Tj
3.7246 0 Td
(2way)Tj
2.2677 0 Td
(ANOVA)Tj
3.7644 0 Td
(Sidak's)Tj
2.9991 0 Td
(test\))Tj
1.9388 0 Td
(compared)Tj
4.2463 0 Td
(to)Tj
1.0262 0 Td
(its)Tj
1.1451 0 Td
(Tet)Tj
1.5534 0 Td
(control)Tj
3.1238 0 Td
(line,)Tj
1.9275 0 Td
(with)Tj
2.0013 0 Td
(a)Tj
0.6519 0 Td
(slightly)Tj
3.0841 0 Td
(larger)Tj
-33.4542 -1.2983 Td
(reduction)Tj
4.1158 0 Td
(after)Tj
2.0353 0 Td
(12)Tj
1.1678 0 Td
(weeks)Tj
2.6079 0 Td
(\(p)Tj
/F16 1 Tf
0 Tc
(<)Tj
/F9 1 Tf
-0.004 Tc
(0.0001\))Tj
4.7962 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1C)Tj
0 g
(\).)Tj
/F10 1 Tf
1.9219 0 Td
[()]TJ
/F9 1 Tf
(Ri)Tj
1.7745 0 Td
(and)Tj
/F10 1 Tf
1.7291 0 Td
[()]TJ
/F9 1 Tf
(MelCS)Tj
3.583 0 Td
(lines)Tj
2.0693 0 Td
(displayed)Tj
3.9798 0 Td
(similar)Tj
2.982 0 Td
(hatch)Tj
-34.5824 -1.3039 Td
(rates)Tj
2.1089 0 Td
(\()Tj
/F16 1 Tf
0 Tc
(>)Tj
/F9 1 Tf
-0.004 Tc
(60%\))Tj
3.3789 0 Td
(across)Tj
2.6759 0 Td
(all)Tj
1.1452 0 Td
(ages)Tj
1.9048 0 Td
(until)Tj
2.109 0 Td
(week)Tj
2.245 0 Td
(10)Tj
1.1735 0 Td
(\(no)Tj
1.6158 0 Td
(significant)Tj
4.388 0 Td
(difference)Tj
4.2066 0 Td
(in)Tj
1.0318 0 Td
(hatch)Tj
2.4321 0 Td
(rates)Tj
2.109 0 Td
(compared)Tj
4.2462 0 Td
(to)Tj
-36.7707 -1.2983 Td
(the)Tj
1.4683 0 Td
(respective)Tj
4.1839 0 Td
(Tet)Tj
1.5534 0 Td
(control)Tj
3.1237 0 Td
(lines)Tj
2.0636 0 Td
(until)Tj
2.109 0 Td
(week)Tj
2.245 0 Td
(8\).)Tj
1.2643 0 Td
(By)Tj
1.2529 0 Td
(contrast,)Tj
/F10 1 Tf
3.6907 0 Td
[()]TJ
/F9 1 Tf
(Pip)Tj
2.2223 0 Td
(eggs)Tj
1.9389 0 Td
(appeared)Tj
3.8664 0 Td
(to)Tj
1.0262 0 Td
(deteriorate)Tj
-32.0086 -1.2982 Td
(rapidly,)Tj
3.2598 0 Td
(with)Tj
/F16 1 Tf
0 Tc
2.0012 0 Td
(<)Tj
/F9 1 Tf
-0.004 Tc
0.9921 0 Td
(20%)Tj
1.9276 0 Td
(hatch)Tj
2.4321 0 Td
(rate)Tj
1.7461 0 Td
(observed)Tj
3.7927 0 Td
(after)Tj
2.0353 0 Td
(3)Tj
0.6973 0 Td
(weeks)Tj
2.6079 0 Td
(of)Tj
1.0148 0 Td
(aging)Tj
2.4037 0 Td
(\(significant)Tj
4.7282 0 Td
(reductions)Tj
4.473 0 Td
(at)Tj
0.9525 0 Td
(all)Tj
-35.0643 -1.304 Td
(weeks)Tj
2.6078 0 Td
(tested,)Tj
2.7779 0 Td
(p)Tj
/F16 1 Tf
0 Tc
(<)Tj
/F9 1 Tf
-0.004 Tc
(0.0001,)Tj
4.337 0 Td
(compared)Tj
4.2463 0 Td
(to)Tj
/F10 1 Tf
1.0204 0 Td
[()]TJ
/F9 1 Tf
(Pip.Tet\).)Tj
4.354 0 Td
(Together,)Tj
4.0706 0 Td
(these)Tj
2.2563 0 Td
(data)Tj
1.9106 0 Td
(indicate)Tj
3.4129 0 Td
(a)Tj
0.6519 0 Td
(substantial)Tj
4.5071 0 Td
(fit-)Tj
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(ness)Tj
1.9105 0 Td
(cost)Tj
1.8141 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
/F10 1 Tf
1.474 0 Td
[()]TJ
/F9 1 Tf
(Pip)Tj
2.2224 0 Td
(line.)Tj
ET
Q
q
0.45 0.36 0.35 0.01 k
1 j
1 J
0 w
36 703.8893 m
576 703.8893 l
576 703.3893 l
36 703.3893 l
f*
106.6277 703.3893 m
106.6277 690.8893 l
106.1277 690.8893 l
106.1277 703.3893 l
f*
106.1277 690.8893 m
36 690.8893 l
36 691.3894 l
106.1277 691.3894 l
f*
223.8089 703.3893 m
223.8089 690.8893 l
223.3089 690.8893 l
223.3089 703.3893 l
f*
223.3089 690.8893 m
106.6277 690.8893 l
106.6277 691.3894 l
223.3089 691.3894 l
f*
343.3158 703.3893 m
343.3158 690.8893 l
342.8158 690.8893 l
342.8158 703.3893 l
f*
342.8158 690.8893 m
223.8089 690.8893 l
223.8089 691.3894 l
342.8158 691.3894 l
f*
575.9997 690.8893 m
343.3158 690.8893 l
343.3158 691.3894 l
575.9997 691.3894 l
f*
106.6277 690.8893 m
106.6277 678.3894 l
106.1277 678.3894 l
106.1277 690.8893 l
f*
106.1277 678.3894 m
36 678.3894 l
36 678.8894 l
106.1277 678.8894 l
f*
223.8089 690.8893 m
223.8089 678.3894 l
223.3089 678.3894 l
223.3089 690.8893 l
f*
223.3089 678.3894 m
106.6277 678.3894 l
106.6277 678.8894 l
223.3089 678.8894 l
f*
343.3158 690.8893 m
343.3158 678.3894 l
342.8158 678.3894 l
342.8158 690.8893 l
f*
342.8158 678.3894 m
223.8089 678.3894 l
223.8089 678.8894 l
342.8158 678.8894 l
f*
575.9997 678.3894 m
343.3158 678.3894 l
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575.9997 678.8894 l
f*
106.6277 678.3894 m
106.6277 665.8894 l
106.1277 665.8894 l
106.1277 678.3894 l
f*
106.1277 665.8894 m
36 665.8894 l
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106.1277 666.3895 l
f*
223.8089 678.3894 m
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223.3089 665.8894 l
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f*
223.3089 665.8894 m
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106.6277 666.3895 l
223.3089 666.3895 l
f*
343.3158 678.3894 m
343.3158 665.8894 l
342.8158 665.8894 l
342.8158 678.3894 l
f*
342.8158 665.8894 m
223.8089 665.8894 l
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342.8158 666.3895 l
f*
575.9997 665.8894 m
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575.9997 666.3895 l
f*
106.6277 665.8894 m
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106.1277 653.3895 l
106.1277 665.8894 l
f*
106.1277 653.3895 m
36 653.3895 l
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106.1277 653.8895 l
f*
223.8089 665.8894 m
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223.3089 653.3895 l
223.3089 665.8894 l
f*
223.3089 653.3895 m
106.6277 653.3895 l
106.6277 653.8895 l
223.3089 653.8895 l
f*
343.3158 665.8894 m
343.3158 653.3895 l
342.8158 653.3895 l
342.8158 665.8894 l
f*
342.8158 653.3895 m
223.8089 653.3895 l
223.8089 653.8895 l
342.8158 653.8895 l
f*
575.9997 653.3895 m
343.3158 653.3895 l
343.3158 653.8895 l
575.9997 653.8895 l
f*
0.83 0.64 0.02 0 k
36 640.2898 m
185.0457 640.2898 l
h
f*
0 g
7.9999 0 0 7.9999 36 708.7181 cm
BT
/F3 1 Tf
1 TL
-0.005 Tc
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