A genome-wide screen of Epstein-Barr virus proteins that modulate host SUMOylation identifies a SUMO E3 ligase conserved in herpesviruses
Fig 5
Effect of SM, UL54 and UL69 on global SUMO1 and SUMO2 modifications.
293T (A) and CNE2Z (C) cells were co-transfected with plasmids expressing His6-SUMO1 (left panels) or His6-SUMO2 (right panels) and either ICP0, LMP1, FLAG-SM, FLAG-UL54, FLAG-UL69 or empty vector control. HeLa cells (B) stably expressing His6-SUMO1 (left panels) or His6-SUMO2 (right panels) were transfected with plasmids expressing ICP0, LMP1, FLAG-SM, FLAG-UL54, FLAG-UL69 or empty vector control. His6-tagged proteins were recovered as in Fig 1 and immunoblotted for SUMO1 or SUMO2 as indicated. Samples of the input lysates were also immunoblotted for actin and FLAG.