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A genome-wide screen of Epstein-Barr virus proteins that modulate host SUMOylation identifies a SUMO E3 ligase conserved in herpesviruses

Fig 1

Screens of EBV proteins for global effects on cellular SUMO1 and SUMO2 modifications.

A. 293T cells were co-transfected with plasmids expressing His6-SUMO1 (top panels) or His6-SUMO2 (bottom panels) and the indicated viral protein or empty vector control. B. HeLa cells stably expressing His6-SUMO1 (left panels) or His6-SUMO2 (right panels) were transfected with plasmids expressing the indicated viral protein or empty vector control. C. CNE2Z cells were co-transfected with plasmids expressing His6-SUMO1 (left panels) or His6-SUMO2 (right panels) and the indicated viral protein or empty vector control. In all cases, His6-tagged proteins were recovered from cell lysates on metal chelating resin under denaturing conditions (Pull-down panels) and immunoblotted for SUMO1 or SUMO2 as indicated. Samples of the lysates (Inputs) were also immunoblotted for actin and FLAG, to detect the FLAG-tagged EBV library proteins. Note that LMP1 and ICP0 did not contain FLAG-tags and hence are not seen in the FLAG blots.

Fig 1

doi: https://doi.org/10.1371/journal.ppat.1007176.g001