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KSHV-induced ligand mediated activation of PDGF receptor-alpha drives Kaposi's sarcomagenesis

Fig 3

KSHV-mediated PDGF upregulation and PDGFRA activation in mECK36 cells and tumors.

(A) Fold-changes in KSHV gene expression and PDGFRA ligands between mECK36 cells and mECK36 tumors determined by RT-qPCR in triplicate and are presented as means ± SD. *P < 0.05. (B) Total and phospho-PDGFRA together with its ligands PDGFA and PDGFB determined by immunoblotting in mECK36 cells (duplicate) and three mECK36 tumors from 3 different mice.(C) Total and phospho-STAT3 together with Total and phospho-AKT were determined by immunoblotting in mECK36 cells (duplicate) and three mECK36 tumors from 3 different mice.(D) Fold-changes in PDGFRA ligands and KSHV gene expression between doxyxcyclin induced and un-induced mECK36 cells stably transfected with a Tet-inducible RTA were measured by RT-qPCR after 24 hours of induction. Data were from three independent experiments carried out in triplicate and are presented as means ± SD. *P < 0.05.

Fig 3

doi: https://doi.org/10.1371/journal.ppat.1007175.g003